• Title/Summary/Keyword: rap

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AtERF73/HRE1, an Arabidopsis AP2/ERF Transcription Factor Gene, Contains Hypoxia-responsive Cis-acting Elements in Its Promote (애기장대의 AP2/ERF 전사인자인 AtERF73/HRE1의 프로모터에 있어서 저산소 반응 cis-조절 요소의 분석)

  • Hye-Yeon Seok;Huong Thi Tran;Sun-Young Lee;Yong-Hwan Moon
    • Journal of Life Science
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    • v.33 no.1
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    • pp.34-42
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    • 2023
  • In a signal transduction network, from the perception of stress signals to stress-responsive gene ex- pression, binding of various transcription factors to cis-acting elements in stress-responsive promoters coordinate the adaptation of plants to abiotic stresses. Among the AP2/ERF transcription factor family genes, group VII ERF genes, such as RAP2.12, RAP2.2, RAP2.3, AtERF73/HRE1, and AtERF71/ HRE2, are known to be involved in the response to hypoxia stress in Arabidopsis. In this study, we dissected the HRE1 promoter to identify hypoxia-responsive region(s). The 1,000 bp upstream promoter region of HRE1 showed increased promoter activity in Arabidopsis protoplasts and transgenic plants under hypoxia conditions. Analysis of the promoter deletion series of HRE1, including 1,000 bp, 800 bp, 600 bp, 400 bp, 200 bp, 100 bp, and 50 bp upstream promoter regions, using firefly luciferase and GUS as reporter genes indicated that the -200 to -100 region of the HRE1 promoter is responsible for the transcriptional activation of HRE1 in response to hypoxia. In addition, we identified two putative hypoxia-responsive cis-acting elements, the ERF-binding site and DOF-binding site, in the -200 to -100 region of the HRE1 promoter, suggesting that the expression of HRE1 might be regulated via the ERF transcription factor(s) and/or DOF transcription factor(s). Collectively, our results suggest that HRE1 contains hypoxia-responsive cis-acting elements in the -200 to -100 region of its promoter.

Resistance Consciousness of Korea's Popular Music - Comparative analysis in the Folk music and Rap Music - (한국 대중음악 속의 저항의식 - Folk와 Rap Music 속의 저항의식 비교분석 -)

  • Choi, Yeong-Seon
    • Proceedings of the KAIS Fall Conference
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    • 2011.12a
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    • pp.97-100
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    • 2011
  • 본 고에서는 포크음악과 힙합(랩)음악의 기본정신에 대하여 알아보고, 이에 따른 시대적 호응에 따른 저항의식을 알아보고 그러한 저항의식이 시대적으로 포크와 힙합(랩)음악에 얼마나 그리고 어떻게 적용이 되고 묻어나 있는지에 대하여 비교분석하여 알아보고자 한다.

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Identification of Sex-specific Expression Markers in the Giant Tiger Shrimp (Penaeus monodon)

  • Khamnamtong, Bavornlak;Thumrungtanakit, Supaporn;Klinbunga, Sirawut;Aoki, Takashi;Hirono, Ikuo;Menasveta, Piamsak
    • BMB Reports
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    • v.39 no.1
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    • pp.37-45
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    • 2006
  • Bulked segregant analysis (BSA) and AFLP were used for isolation of genomic sex determination markers in Penaeus monodon. A total of 256 primer combinations were tested against 6-10 bulked genomic DNA of P. monodon. Five and one candidate female- and male-specific AFLP fragments were identified. Female-specific fragments were cloned and further characterized. SCAR markers derived from FE10M9520, FE10M10725.1, FE10M10725.2 and FE14M16340 provided the positive amplification product in both male and female P. monodon. Further analysis of these markers using SSCP and genome walk analysis indicated that they were not sex-linked. In addition, sex-specific (or differential) expression markers in ovaries and testes of P. monodon were analyzed by RAP-PCR (150 primer combinations). Twenty-one and fourteen RAP-PCR fragments specifically/differentially expressed in ovaries and testes of P. monodon were successfully cloned and sequenced. Expression patterns of 25 transcripts were tested against the first stranded cDNA of ovaries and testes of 3-month-old and broodstock-sized P. monodon (N = 5 and N = 7 - 10 for females and N = 4 and N = 5 - 7 for males, respectively). Five (FI-4, FI-44, FIII-4, FIII-39 and FIII-58) and two (M457-A01 and MII-51) derived RAP-PCR markers revealed female- and male-specific expression patterns in P. monodon. Surprisingly, MII-5 originally found in testes showed a higher expression level in ovaries than did testes of juvenile shrimps but a temporal female-specific pattern in P. monodon adults.

Changes of Triglyceride Composition in Adlay Powder during Storage (율무가루 저장 중 Triglyceride 조성의 변화)

  • Han, Ji-Sook;Rhee, Sook-Hee;Cheigh, Hong-Sik
    • Korean Journal of Food Science and Technology
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    • v.23 no.1
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    • pp.102-108
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    • 1991
  • Raw adlay powder(RAP)was prepared and the changes of triglyceride composition in RAP lipid during storage at $35^{\circ}C$ for six months were studied. The RAP lipid consisted of 28 kinds of triglycerides and the major triglyceride in RAP lipid were those of OOL(24.14%), OLL(24.06%), OOO(12.58%), POL(9.01%), POO(8.87%), LLL(7.91%)and PLL(5.80%). During the storage at $35^{\circ}C$ for six months, the relative amounts of the triglyceride containing linoleic acid(OLL : 15.25%, LLL : 2.93%)considerably decreased, but those containing oleic acid(OOO : 23.77%. POO : 12.62%) increased. The triglycerides of LLA(0.44%)and PPLn(0.12%)disappeared during the storage.

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The Behavior of Rammed Aggregate Piers (RAP) in Soft Ground (I) (연악지반의 쇄석다짐말뚝에 대한 거동 분석 (I))

  • Bae, Kyung-Tae;Lee, Chong-Kyu
    • Journal of the Korean Geotechnical Society
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    • v.23 no.4
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    • pp.169-183
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    • 2007
  • Numerical analysis was performed to investigate the behavior of rammed aggregate piers (RAP) in soft ground with various interface conditions, area replacement ratio, aspect ratio and surcharge loads of pile and soil. And field modulus load test was carried out to predict the input parameters. Field prototype (unit cell) tests are in progress to compare the result of numerical analysis. Also a modified load transfer equation of RAP on soft foundation was proposed. According to the results, the behavior of RAP depended on such as interface conditions, settlement characteristics (free strain) and stress concentration ratio. On the other hand, maximun stress concentration ratio increased as area replacement ratio and aspect ratio increased, and it was remarkably affected by surcharge loads.

A PSYCHIATRIC STUDY ON THE CHILDREN WITH RECURRENT ABDOMINAL PAIN (반복적 복통 환아의 정신과적 연구(反復的 腹痛 患兒의 精神科的 硏究))

  • Choi, Jin-Sook;Hong, Kang-E;Seo, Jeong-Kee
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.1 no.1
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    • pp.117-129
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    • 1990
  • 35 children(17 boys, 18 girls) with Recurrent Abdominal pain(RAP) without apparent organic causes, referrd from pediatric department of Seoul National University Children's Hospital were studied during 1988. 7. 1 ${\sim}$ 1989. 2. 28. The aims of this study are to examine clinical characteristics of the patients with RAP, to find possible factors influencing the onset and the course of the disorder, to evaluate the psychological status and the relationship with psychiatric diagnoses. The study subjects were compared with the age and sex matched control subjects on measures of social and school functioning, family-environment, behavior traits by CBCL and parent-environment rating scale. All patients and their mothers were interviewed by a child psychiatrist. The results were as follows ; 1) Abdominal pain was found to be more frequent when with a care taker, and related with traumatic life stresses such as separation from parents, death of family members. 2) Family members of the patients with RAP also had a high rate of a history of gastrointestinal dysfunctions with could not be attributed to organic etiologies. 3) Among 35 patients with RAP, 16 were diagnosed as psychiatric disorders. They were adjustment disorders (4), overanxious disorders (4), depression (2), tic disorders (2), attention deficit disorders (2), separation anxiety disorders (1), enuresis (1). 4) On CBCL, the patients with RAP were more internalized, socially less competent and less active compared with the control subjects. 5) According to the parent-environment rating scale, the patients with RAP had more conflicts with their parents about control issues. Family members of the patients with RAP were socially less competent and less abjustable. The parents of the patients with RAP were more affectionate and spent more time with their children but they were unfair and vague on instruction and discipline.

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Analysis of Changes in Hemodynamic Values after Modified Fontan Procedure (변형 Fontan 술후 혈류역학치 변화추이 분석)

  • 안재호
    • Journal of Chest Surgery
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    • v.21 no.5
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    • pp.816-827
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    • 1988
  • Since 1978, We have experienced 87 cases of Fontan operations and the candidates of that increased in numbers recently with the improvement of the diagnostic and operative technique. We studied the prerequisite factors and hemodynamics of 22 cases of Fontan operations, done during the last one year period, which were 3 tricuspid atresia, 16 functional single ventricle and 3 anatomic single ventricle. The mean age was 68 months and the mortality rate 24%, and 9 patients of under 4 years of age were operated with 22.2% mortality rate, but the youngest, 16 months of age, patient survived well without problems. The preoperative pulmonary artery pressure[PAP], pulmonary vascular resistance[PVR] and postoperative right atrial pressure[RAP], left atrial pressure[LAP] value influenced the mortality, but age, preoperative Hb, preoperative PaO2 and pulmonary artery index[PAI] did not. There were favorable survival tendency in under 15mmHg of preop. PAP, 2a of preop. PVR and under 25cmHyO of postop. RAP, under 15cmHyO of postop LAP. The younger, the more pleural effusion and the longer postoperative admission days. The higher preop. Hb related to the higher postop. transpulmonary pressure gradient and the lower preop. PaO2 and PAI. The higher preop. PaO2, the less pleural effusion and postop. admission days. Preop. PAP closely related to preop. PVR and postop. LAP and high PVR increased the pleural effusion and postop. admission days. The larger PAI, the larger CI. We concluded that there were so many factors influencing the postoperative condition, but preop. PAP, PVR, Hb, postop. RAP and LAP were the most ones.

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The Preload-Responsive Regulation of Cardiac Output in Total Artificial Heart Using Dual Adaptive Controller (2중 적응제어방식에 의한 전치환 인공심장의 전부하에 민감한 심박출량 조절)

  • Lee, Sang-Hoon;Kim, In-Young;Ahn, Hyuk;Min, Byoung-Goo
    • Journal of Biomedical Engineering Research
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    • v.14 no.1
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    • pp.41-50
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    • 1993
  • This paper proposes an adaptive technique for the cardiac output regulation of a pendulum type electromechanical TAH. This techinque, which consists of two RLSE's and two PASTC' 5, performs Its controllability over the TAIB so that the m(RAP) and m(LAP) values re- main close to their desired values under she assumption that the variation of m(RAP) and m (LAP) are dominated by the variation of C.0. and the difference between the left and right ventricular output, respectively. To evaluate the performance of the proposed control system, a simulation is performed by using a human model which contains physiologic, drug and treatment, artificial heart and noise models. As a result, dual adaptive controller showed that abnormal m(LAP) and m(RAP) could be recovered to the normal range within 10minute and maintained desired value in steady state. The operation of this controller prored to be robust in spite of the rapid variation of human status.

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Propellant Characteristics used for a Rocket-Assisted Projectile with Aluminium Contents (알루미늄 함량에 따른 로켓보조추진탄용 추진제 특성)

  • Jeong, Jae-Yun;Choi, Sung-Han
    • Journal of the Korean Society of Propulsion Engineers
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    • v.23 no.5
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    • pp.60-66
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    • 2019
  • In this report, the process characteristic(viscosity), mechanical properties, combustion characteristics, ground and flight test results of propellants used for a rocket-assisted projectile are described according to several aluminum contents. As the aluminum content increased, initial viscosity decreased, viscosity build-up accelerated, and combustion rate and pressure exponent decreased. In the ground fire test, the total impulse of the rocket-assisted projectiles containing 10 wt% of aluminum were 5% higher than that of the rocket-assisted projectiles containing 2 wt% and 18 wt% of aluminum. The motor efficiency compared to the theoretical performance was 85.6% with 18 wt% of aluminum, the lowest value among the propellant compositions.

Correlation of Binder GPC Characteristics and Mechanical Properties of Hot-Mix Recycled Asphalt Mixtures (재생혼합물의 바인더 GPC특성과 역학적 특성과의 상관성 연구)

  • Kim, Kwang-Woo;Hong, Sang-Ki;Cho, Mun-Jin;Doh, Young-Soo
    • International Journal of Highway Engineering
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    • v.7 no.1 s.23
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    • pp.11-20
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    • 2005
  • This study examined the correlation between mechanical properties and LMS(Large molecular size) of binders in hot-recycled asphalt mixtures. Hot-recycled asphalt mixtures were manufactured by various mixing methods. Laboratory tests including indirect tensile strength, wheel tracking test and Kim test were performed for each recycled mixture. Gel-permeation chromatography (GPC) analysis was performed for the binders coated on virgin and old (RAP) aggregates separately. For the purpose of binder aging analysis, a round-shaped virgin coarse aggregates (13mm gravel) were introduced in recycled mixtures. This makes possible to distinguish the virgin aggregates from RAP aggregates in recycled mixtures for GPC sampling. Results of GPC showed that there was significant difference in aging level between the binder coated on RAP and that of virgin aggregates in the same recycled mixture. Regression analysis was performed to correlate mechanical properties to LMS ratio. Results showed that most of the mechanical properties had relatively good correlation with LMS. This trend agree with LMS increase up to some degree, but fails for further LMS increase.

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