• Animal cells and systems
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• v.3 no.1
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• pp.73-78
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• 1999

Although there are several methods for the preparation of mitochondrial DNA (mtDNA) from mammalian tissues, most are relatively long ultracentrifugation or manipulations by a small-scale method. We escribed a rapid method for large-scale extraction of mtDNA from human placental and horse liver tissues. The method is based on the preparation and homogenization of tissues, urification of crude mitochondria by differential centrifugations and isolation of mtDNA by alkaline Iysis. It was improved from Pre-existing methods by replacing some steps with simpler ones and discarding many others. This method gives a high yield of pure mtDNA(approximately 1-5mg from one placenta; ca. 400-600 g wet weight), depending on its sources (fresh tissue gave better results than frozen one). The resulting mtDNA indicated that this method can yield mtDNA in sufficient purity and quantity to identify the direct restriction analysis on agarose gel, random-primed labeling as a probe, and end labeling. Therefore, the method is ideal for obtaining good mtDNA samples to conduct routine restriction fragment length polymorphism (RFLP) analyses of natural populations for genetic studies.

• Journal of Institute of Control, Robotics and Systems
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• v.7 no.12
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• pp.1015-1023
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• 2001

error behaviour can be considered as a linear combination of low amplitude random noise and abrupt jumps. The reason of jump appearance can be explained by the semi-shading effects(buildings, trees), jamming, high dynamic of vehicle and so on. This study describes the stand-alone GPS error jump smoothing algorithm which is developed based on the scalar adaptive filter. The algorithm consists of the coarse jump smoothing and the fine jump smoothing. On the coarse smoothing step, GPS velocities or position differences are used as the measurement for the scalar adaptive filter. The purpose of adaptive filter is to smooth the jump errors. The coarse positions are detennined by the integration of smoothed velocities. On the fine smoothing step, the differences between GPS positions and the coarse positions are smoothed by another scalar adaptive filter. The reason of fine smoothing is based on the facts that smoothing accuracy depends on the variance ofusefuJ signa\. The coarse smoothing which deal with the difference of positions provides the rough error removing. So the coarse smoothed velocities can have much more low amplitude than the raw ones. The fine smoothing procedure provides high quality of filtering process. Simulation results show the efficiency of proposed scheme.

• Journal of the Earthquake Engineering Society of Korea
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• v.13 no.4
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• pp.37-45
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• 2009

The identification of significant structural components under seismic loading through a probabilistic approach is of interest to many structural engineers. The First-Order Second Moment method can be used to achieve this goal by estimating uncertainty in the seismic demand of a structural system induced by the capacity uncertainties of each structural component. Significant structural components are those to which the seismic demand of the structure is more sensitive than it is to other ones. The developed procedure demonstrated by a ductile reinforced concrete frame shows that it is computationally effective and robust in terms of identifying significant structural components.

• Structural Engineering and Mechanics
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• v.54 no.6
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• pp.1217-1244
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• 2015

In this research a theoretical and numerical study on a bridge damage detection procedure is presented based on vibration measurements collected from a set of accelerometers. This method, referred to as "Adjoint Variable Method", is a sensitivity-based finite element model updating method. The approach relies on minimizing a penalty function, which usually consists of the errors between the measured quantities and the corresponding predictions attained from the model. Moving mass is an interactive model and includes inertia effects between the model and mass. This interactive model is a time varying system and the proposed method is capable of detecting damage in this variable system. Robustness of the proposed method is illustrated by correct detection of the location and extension of predetermined single, multiple and random damages in all ranges of speed and mass ratio of moving vehicle. A comparative study on common sensitivity and the proposed method confirms its efficiency and performance improvement in sensitivity-based damage detection methods. In addition various possible sources of error, including the effects of measurement noise and initial assumption error in stability of method are also discussed.

• Coupled systems mechanics
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• v.3 no.2
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• pp.147-170
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• 2014

In the recent times, dimensions of heavy load carrying vehicle have changed significantly incorporating structural flexibility in vehicle body. The present paper outlines a procedure for the estimation of bridge response statistics considering structural bending modes of the vehicle. Bridge deck roughness has been considered to be non homogeneous random process in space. Influence of pre cambering of bridge surface and settlement of approach slab on the dynamic behavior of the bridge has been studied. A parametric study considering vehicle axle spacing, mass, speed, vehicle flexibility, deck unevenness and eccentricity of vehicle path have been conducted. Dynamic amplification factor (DAF) of the bridge response has been obtained for several of combination of bridge-vehicle parameters. The present study reveals that flexible modes of vehicle can reduce dynamic response of the bridge to the extent of 30-37% of that caused by rigid vehicle model. However, sudden change in the bridge surface profile leads to significant amount of increment in the bridge dynamic response even if flexible bending modes remain active. The eccentricity of vehicle path and flexural/torsional rigidity ratios plays a significant role in dynamic amplification of bridge response.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.42 no.3
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• pp.562-565
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• 2017

In this letter, we consider a 7-step transmission procedure of a large number of machine nodes when they simultaneously request random access to transmit uplink data. We model the radio resource utilization of LTE systems, and analyze the overloaded resources. From the simulation results, we show that the resource of PDCCH becomes significantly overloaded as the number of machine nodes increases in a cell. To alleviate the overload of PDCCH, we allocate radio resource of PDSCH to PDCCH. The result shows that the resource utilization of PDCCH is improved.

• The Korean Journal of Applied Statistics
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• v.11 no.2
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• pp.461-475
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• 1998

In panel survey in which the sample is selected by stratified random sampling, if the sampling units shift from a stratum to others in time, then the movement should be incorporated in the estimation procedures. Dealing with the problem caused by the movement of units across stratum in the updated stratification sample, the bias of the conventional estimator neglecting the movement is investigated, arid the bias-adjusted estimators are proposed. The variance estimator of the suggested estimators is also derived. It is illustrated via a simulation study that the proposed estimators beat the conventional estimator in the sense of bias and mean squared error In particular, when the Neyman allocation is applied in stratified sampling, the proposed estimator is shown much more effective to this end.

• The Korean Journal of Applied Statistics
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• v.20 no.3
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• pp.605-618
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• 2007

Adaptive sampling design is the selection procedure which depends on observed values of the variable of interest. It is the method which could be applied to the rare and unapproachable population. Adaptive cluster sampling strategies are more efficient than simple random sampling on equivalent sample size. Adaptive sampling with new estimators through the Rao-blackwell method have lower variance than Horvitz-Thompson (HT) and Hansen-Hurwitz (HH). Also, to determine suitable sample size, it was used expected sample and the method finding appropriate sample size by changing initial sample size were studied.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.6
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• pp.2838-2858
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• 2019

Traditional channel models for vehicle-to-vehicle (V2V) communication usually assume fixed velocity in static scattering environment. In the realistic scenarios, however, time-variant velocity for V2V results in non-stationary statistical properties of wireless channels. Dynamic scatterers with random velocities and directions have been always utilized to depict the non-stationary statistical properties of the channel. In this paper, a non-stationary geometry-based cooperative scattering channel model is proposed for multiple-input multiple-output (MIMO) V2V communication systems, where a birth-death process is used to capture the appearance and disappearance dynamic properties of moving scatterers that reflect the time-variant time correlation and Doppler spectrum characteristics. Moreover, our model has more straight and concise to study the impact of the vehicular traffic density on channel characteristics and thus avoid complicated procedure in deriving the analytical expressions of the channel parameters and functions. The numerical results validate our analysis and demonstrate that setting important parameters of our model can appropriately build up more purposeful measurement campaigns in the future.

• Journal of the Korea Institute of Military Science and Technology
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• v.24 no.3
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• pp.302-308
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• 2021

Developers generally use test standards suggested by military standards such as MIL-STD-810G when performing vibration tests in the materiel development. However, according to MIL-STD-810G, it is recommended to test by tailoring the test standard suitable for the developed materiel, and it is specified to apply the suggested test standard only when there is difficulty in tailoring. In addition, the test standards presented by MIL-STD-810G are standards created under operating conditions different from the actual operating environment of each developed materiel, so the test according to this standard may be excessive or understated. Therefore, the developer must create an appropriate vibration test standard for the developed materiel as similar to the operating conditions as possible. In this paper, the procedure for creating the functional test standard and durability test standard suitable for the operating environment of the equipment to be mounted on the propeller aircraft under development is described, and the created standard is introduced.