• 미생물학회지
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• 제22권4호
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• pp.249-255
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• 1984

To develop the host-vector system for industrial Coryneform bacteria that seemed to be the most suitable microorganisms for molecular breeding of genes involved in the production of amion acids, nucleotides, and other products of industrial interest, broad host range E. coli plasmid R 1162 DNA was transformed into Brevibacterium ammoniagenes and the plasmids pKU6 isolated from a transformant was physically characterized. All other plasmids from the transformed cells except pKU6 exsisted as multimeric forms in Brevibacterium ammoniagenes. The plasmid DNA was retransformed into Corynebacterium glutamicum with a high frequency ($1.32{\times}10^{-1}$ per cell) and maintained stably both in Brevibacterium ammoniagenes and Corynebacterium glutamicum after 100 generations of cultures with 25-30 copy number per cell. The size of both plasmid pKU6 and plasmid R1162 were the same and restriction maps by EcoR I, Ava I, Pst I, Pvu II and Hinc II were also similar.

• 미생물학회지
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• 제26권3호
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• pp.167-172
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• 1988

A genetic map of the IncP-1 group plasmid pKU10 has been prepared through the construction of recombinant plasmids containing various fragments of pKU10. Phenotypic analysis of these derivatives has identified the location of genes encoding resistance to ampicillin, tetracyclin, and chloramphenicol. The region involved in conferring resistance to ampicillin was located around two PstI sites that are 1.0Kb apart. The tetracyclin resistance gene was mapped on the region of HindIII E fragment and a part of HindIII D fragment, and the determinant for chloramphenicol resistance gene was localized on HindIII D fragment.

• 동의신경정신과학회지
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• 제25권1호
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• pp.55-62
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• 2014

Objectives: The purpose of this study is to investigate the relationship of nicotine dependence and mental health. Methods: The subjects of this study were 153 male smokers in their 20s, 30s, 40s and 50s who visited Wonmi Public Health Center. They were evaluated with Fagerstorm's test for nicotine dependence (FTND) and Symptom Checklist-90-Revision (SCL-90-R). Using the FTND score, the participants were divided into three groups; mild nicotine dependent group, moderate group and severe group. Results: In the SCL-90-R score, there were statistically significant differences in the characteristics of Somatization, Obsessive-Compulsive, Interpersonal Sensitivity, Depression, Global Severity Index (GSI) and Positive Symptom Test (PST) among the three groups. Comparing the values of FTND and the amounts of Daily Smoking to the values of Somatization, Obsessive-Compulsive, Interpersonal Sensitivity, Depression and GSI in this study resulted in positive correlations, thereby demonstrating a statistical significance. Conclusions: The above results suggest that there are significant relations between nicotine dependence and the level of mental health. These results can be used for planning the smoking cessation program and mental health promoting program based on Korean traditional medicine.

• 한국미생물·생명공학회지
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• 제14권3호
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• pp.245-250
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• 1986

청주시 무심천의 하천수에서 항생물질에 내성을 나타내는 Gram 음성 세균을 분리하여 수질환경에서 일어나는 R 플라스미드의 전이를 연구하였다. 분리된 균주사이에서 접합에 의한 R 플라스미드의 전이는 실험실 환경에서 1.1$\times$$10^{-6}$-1.2$\times$$10^{-7}$, 하천의 수질환경에서 1.2$\times$$10^{-7}$-1.0$\times$$10^{-9}$으로 나타나, 자연의 수질환경에서도 R 플라스미드의 전이가 일어남을 확인하였다. 또 T-44 균주의 Ap$^{r}$Cm$^{r}$Tc$^{r}$ 플라스미드는 형질전환에 의하여 E. coli HB 101에 1.7$\times$$10^{-6}$의 비율로 전이되었다. 분자의 크기가 약 9.01kb로 측정된 Ap$^{r}$Cm$^{r}$Tc$^{r}$플라스미드 DNA를 제한효소로 처리한 결과 이 플라스비드에는 EcoRI과 BamHI의 절단부위가 각각 하나씩 존재하고 P-stI의 절단부위는 3개가 있었다.

• 대한가정학회지
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• 제36권10호
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• pp.179-196
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• 1998

The purpose of this study was to analyze the financial status of Korean elderly households to make a financial plan for retirement preparation. The sample obtained from 1997 KHPS, consisted of 326 households of 55-64 aged and 210 households of 65 and over aged. Statistics employed for the analysis were frequencies, percentile, univariate analysis and logistic analysis. And Lorenz curve and Gini coefficient were used to compare the financial status. The results could be summarized as follows. First, total income, total expenditure, total debt and net worth of 55-64 aged group were higher than those of 65 and over group, while total asset of 55-64 aged group was similar to 65 and over group's Second, total expediture to total imcome ratio(R1) and total asset to total debt ratio(R4) of 55-64 aged group were higher than 65 and over group's, but net worth to minimum cost of living ratio(R2) and financial asset to monthly expenditure ratio(R3) of 65 and over group were higher than 55-64 aged group's . Third, R4's Gini coefficient was the highest and R1's Gini coefficient was the lowest among all Gini coefficients. Also, R1's and R4's Gini coefficients of 55-64 aged group were greater than 65 and over group's. ourth, variables affecting R1's stability of 55-64 aged group were household head's age, educatonal attainment and family type, while those of 65 and over group were household head's age, past economic experience and the number of children. There was no significant variable affecting R2's stability of 55-64 aged group, but there was only household head's pst economic experience affecting those of 65 and over group. The variable contributing to R3's stability of 55-64 aged group was residence while the variables of 65 and over group were household head's educational attainment, past economic experience and the number of children. The variables contributing to R4's stability of 55-64 aged group were the number of children and residence, and the number of children was one variable of contributing to R4's stability of 65 and over aged group.

• 대한물리의학회지
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• 제16권1호
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• pp.1-7
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• 2021

PURPOSE: The purpose of this study is to conduct inter-rater and intra-rater reliability tests in patients with low back pain (LBP) using the prone instability test (PIT) and side-lying instability test (SIT). We have analyzed the Korean version Oswestry disability index (K-ODI) correlations and radiograph finding (RF) for validity. METHODS: Individuals (n = 51) (mean age of 40.27 ± 13.28) with LBP for at least over a week were recruited, together with two participating physical therapist examiners. The measurement consisted of PIT, PST, K-ODI, and RF. Sensitivity (Sn), specificity (Sp), positive predictive value, negative predictive value, prevalence index, agreement %, Cohen's kappa, and prevalence-adjusted bias-adjusted kappa (PABAK) were calculated. The PIT and SIT were compared with RF for validity analysis, while PIT, SIT, K-ODI, and RF were calculated for the correlation analysis. RESULTS: The intra-rater reliability test measured for the PIT (kappa = .79, PABAK = .88) and SIT (kappa = .73, PABAK = .84), and inter-rater reliability test measured for the SIT (kappa = .80, PABAK = .88) showed good agreements. The PIT (Sn = .65, Sp = .63) and SIT validities (Sn = .68, Sp = .70) were compared with RF, showing a significant correlation in PIT and RF (r = .69), SIT and RF (r = .73), and PIT and K-ODI (r = .53). CONCLUSION: The SIT is a more comfortable position test than the PIT in patients. Both PIT and SIT have acceptable reliability and validity.

• Journal of Periodontal and Implant Science
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• 제29권4호
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• pp.963-979
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• 1999

This study examined ribotypes of 36 P. gingivalis strains isolated from 10 rapidly progressive periodontitis patients in Korean and revealed the presence of genetic heterogeneity among the patients. Ribotyping was performed by using a oligonucleotide probes based on 16S rRNA after whole genomic DNA had been digested with the restriction endonuclease enzyme Kpn I and Pst I. In addition, the antigenic heterogeneity of fimbrillin and protease activity was analysed to observe the virulency of P. gingivalis. The results were as follows. 1. Using KpnI, 6 ribotypes were detected, whereas 7 ribotypes were identified by using PstI. When combined two enzymes, a total of 8 ribotypes was subgrouped. 2. Ribotype I/e was the most common and detected in 4 among 10 patients. 3. The fimbrillin expressed from P. gingivalis isolates had the molecular size of 41kDa, 43kDa, 49kDa. It was observed that the size of fimbrillin with the same ribotypes could be identical. 4. All the P. gingivalis strains showed strong proteolytic activity and had the molecular size more than 120kDa. In summary, total 8 ribotypes were observed for isolates from rapidly progressive periodontitis patients. Forty percent of the patients harbored isolates exhibiting the same ribotype I/e, and it was observed that more than one ribotype can coexist in an individual patient.

• 한국수정란이식학회지
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• 제18권3호
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• pp.227-235
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• 2003

한우의 mt DNA cytochrome oxidase subunit I, II, 및 III complex지역의 유전적 다형현상을 제한효소를 이용하여 검출하였다. PCR primer 6종에 대하여 20가지 제한효소를 처리하였으며, Pst I, Pvu II, Rsa I, Eco RI, Bgl II, and Msp I 제한효소를 사용하여 유전적 변이를 검출하였다. 검출된 변이체와 한우의 성장과의 관련성을 조사한 결과 cytochrome oxidase subunit III complex 지역의 유전염기서열을 근거로 제작한 primer Mt9 좌위에서 제한효소 PvuII를 이용한 절단형과 체중형질 인 WT15(P<0.05) 및 WT18(P<0.01)에서 고도의 유의성이 관찰되었다. 아울러 , Mt9-Pvu II(P=0.07), Mt6-Bgl II(P=0.05), and Mt8-Rsa I(P=0.05) 좌위 또한 WT9, WTl5, and WT15에서 각각 통계적 유의성이 관찰되었다. 따라서 본 결과는 cytochrome oxidase subunit III complex segments가 candidate gene으로서 기초적 유전정 보 제공은 물론 유전적 개량을 위해 사용될 수 있을 것으로 사료된다.

• 정신신체의학
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• 제10권1호
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• pp.37-47
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• 2002

연구목적 : 수면관련 호흡장애 (sleep-related breathing disorder, SRBD)는 생명을 위협하는 중요한 질환으로 유병율이 매우 높다. 본 연구의 목적은 일차성 코골이 (PS) 환자와 폐쇄성 수면무호흡증 (OSA) 환자들이 보이는 정신의학적 증상들을 조사하는데 있다. 방법 : 동아대학교병원 수연장애 클리닉에 내원, SRBD가 의심되어 수면다원검사를 시행한 환자 중, SRBD로 확진받은 80명 (PS 29명 OSA 51 명)을 연구대상으로 하였다. 다면적 인성검사 (MMPI)와 간이정신진단검사 (SCL-90-R)를 실시하여 PS군과 OSA군의 정신의학적 증상들을 조사 분석하였다. 결과 : 전체 SRBD 환자군은 MMPI의 건강염려증(Hs), 우울증(D), 히스테리(Hy) 척도와 SCL-90-R의 선체화(SOM) 척도의 증가율이 타 척도보다 더 높았다. 이런 현상은 PS와 OSA로 구분하였을 때도 두군 모두에서 3가지 신경증 척도들의 증가율이 높았다 두군간의 비교에서, SCL-90-R의 강박증 (O-C, t=2.14), 우울증 (DEP, t=2.44), 편집증 (PAR, t=2.56), 전체 심도지수 (GSI, t=2.32) 척도(이상 p<0.05) 및 신체화(SOM, t=2.46), 표출 증상합계 (PST, t=2.75) 척도(이상 p<0.01)의 평균점수, 그리고 MMPI의 건강염려증(Hs, t=3.39) 척도와 SCL-90-R 신체화 (SOM, t=6.52) 척도의 증가율이 PS군보다 OSA군에서 유의하게 높았다(모두 p<0.05). 반응 척도와 수면 변인들 간의 상관관계 조사에서, 몇몇 심리 반응 척도들 즉, OSA군의 MMPI(D, Pt, Si) 및 SCL-90-R(ANX, PAR, PSDI) 척도와 PS군의 MMPI(Hy, Pt, Si) 및 SCL-90-R(I-S, PAR. PSDI) 척도, 그리고 PS군의 MMPI 척도 증가율(E')이 수면효율과 서파수면시간 등의 다양한 수면변인들과 상관관계를 보였다. 결론 : 본 연구의 결과는 SRBD 환자들이 신체화 경향을 주로 하는 신경증적 특정을 보이고 있음을 시사한다. 또한 비록 OSA군이 PS군에 비하여 정신의학적 증상을 더 심하게 호소하고 있지만, PS군도 일반인구보다 정신의학적 증상을 더 많이 호소하고 있었다. 이 결과는 OSA군뿐만 아니라 PS군에 대한 정신의학적 관심이 필요함을 시사한다.

• 한국연초학회지
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• 제13권2호
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• pp.34-41
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• 1991

To explore the possibility of introducing Zea mays transposable element Ac(activator) which can be used as a mutagen and gene tag in tobacco plants other than maitre, we tried to introduce a cloned Ac element into tobacco cells by an Agrobacterium tumefaciens binary vector system. Transformation of N. babacum cv. Burley 21 tissues and regeneration to whole plant were carried out. The frequency of the transformed callus induced in shoot induction media was higher than that of transformed callus induced in callus induction media. However, the calli were not grown in the second selection media, and became yellow senescent calli. Regenerated tobacco plantlets with foreign gene were also obtained in shoot induction media containing 100 $\mu\textrm{g}$/ml kanamycin and 100$\mu\textrm{g}$/ml carbenicillin. The leaf tissues of transformant was also resistant to 1000 $\mu\textrm{g}$/ml kanamycin. The chromosomal DNAs of transformant and normal plant of N. tabacum were digested by EcoR I and Hind III but not by Pst I.