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Urease Inhibition and Flocculating Activity of Concentrated Aloe vera Gel by Using Ultrafiltration Process (한외여과 알로에 농축액의 Urease 저해 및 무기물 응집 활성)

  • Baek, Jin-Hong;Kim, Sung-A;Lee, Shin-Young
    • KSBB Journal
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    • v.23 no.3
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    • pp.239-244
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    • 2008
  • For physiological function of aloe concentrate by ultrafiltration (UF) process, jack bean urease inhibitory activity and bentonite flocculating activity of UF aloe concentrate was investigated and compared with fresh aloe gel. Urease inhibitory activity of UF aloe concentrate ranged from 87 to 90% in 1 mL sample. Also, urease inhibitory activity of UF aloe concentrate increased about 10% by heat treatment showing the heat stability. From Lineweaver-Burk plot for UF aloe concentrate, urease inhibition pattern indicated general non-competitive inhibition. From flocculation test of UF aloe concentrate about 1% (w/v) bentonite suspension, maximum flocclulating activity of 97% was obtained at 0.5 mL addition of UF aloe concentrate/ 5 ml bentonite suspension. However, flocculating activity of 81% was obtained at 1 mL addition of UF aloe concentrate/ 5 mL bentonite suspension, which was typical flocculating behavior of polymers with re-dispersion at overdose area. FT-IR spectra of UF aloe concentrate showed the characteristic patterns of $\beta$-binding polysaccharide and less deacetylation indicating higher level of bioactive polysaccharide content.

Studies of the Components of Purple Laver(II) -On Free Fatty Acids- (한국산 "김"의 성분에 관한 연구 (II) -유리 지방산에 대한 연구-)

  • Cook, Chae-Ho;Cho, Youn-Sang;Jew, Sang-Sup
    • Korean Journal of Pharmacognosy
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    • v.5 no.4
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    • pp.211-216
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    • 1974
  • 시판(市販) ${\ulcorner}$${\lrcorner}$의 유리(遊離) 지방산(脂肪酸)을 20% DEGS (diethyleneglycol succinate) 칼람을 장치(裝置)한 가스 크로마토그라피를 사용하여 separation factor 및 oxymereuration-demercuration법(法)을 이용(利用)함으로써 23종(種)의 지방산(脂肪酸)을 확인(確認)하였으며, 가스 크로마로그람의 면적(面積)으로부터 그들을 각각 정량(定量)하였다. 진기(珍奇)한 산(酸)은 존재(存在)하지 않았으며, $C_{22}F{\times}1,\;C_{26}$, oleic acid, palmitic acid가 거의 60%를 차지했다. Oxymercuration-demercuration법(法)에 의(依)하여 분리(分離)된 불포화 지방산(脂肪酸)중에서 $C_{13}F{\times}1,\;C_{18}F{\times}1,\;C_{20}F{\times}1$는 명명(名名) 두 개(個)의 기하이성체(幾何理性體)가 존재하는 것으로 추정(推定)된다. 쇄상 포화 지방산(脂肪酸) 및 $F{\times}1$$F{\times}2 $의 쇄상 불포화 지방산(脂肪酸)은 $R_{USEVA}-A_{TANSONA}$$M_{URRAY}$등이 언급(言及)한 바와 같이 지방산(脂肪酸)의 탄소수(炭素數)에 대해서 $t_R\;(retention time)$을 semi-log plot를 하였을때 직선(直線)을 주었다.

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Transition State Characterization of the Low- to Physiological-Temperature Nondenaturational Conformational Change in Bovine Adenosine Deaminase by Slow Scan Rate Differential Scanning Calorimetry

  • Bodnar, Melissa A.;Britt, B. Mark
    • BMB Reports
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    • v.39 no.2
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    • pp.167-170
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    • 2006
  • Bovine adenosine deaminase undergoes a nondenaturational conformational change at $29^{\circ}C$ upon heating which is characterized by a large increase in heat capacity. We have determined the transition state thermodynamics of the conformational change using a novel application of differential scanning calorimetry (DSC) which employs very slow scan rates. DSC scans at the conventional, and arbitrary, scan rate of $1^{\circ}C/min$ show no evidence of the transition. Scan rates from 0.030 to $0.20^{\circ}C/min$ reveal the transition indicating it is under kinetic control. The transition temperature $T_t$ and the transition temperature interval ${\Delta}T$ increase with scan rate. A first order rate constant $k_1$ is calculated at each $T_t$ from $k_1\;=\;r_{scan}/{\Delta}T$, where $r_{scan}$ is the scan rate, and an Arrhenius plot is constructed. Standard transition state analysis reveals an activation free energy ${\Delta}G^{\neq}$ of 88.1 kJ/mole and suggests that the conformational change has an unfolding quality that appears to be on the direct path to the physiological-temperature conformer.

Effects of Fertilization Methods on Ethylene Evolution and Shattering in Rice Grains (시비법의 벼의 탈입과 Ethylene 생성에 미치는 영향)

  • 박광호;강양순;이재생;정연태
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.4
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    • pp.341-344
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    • 1989
  • The relationship between the rates of rice grain shattering caused by typhoon and characteristics considered to be related with grain shattering such as heading date, amount of grain production and ethylene evolution of rice plant grown under the different methods of fertilization was analyzed. The grain shattering rates which was traced by the fallen grains after typhoon 'Dinah' occured in August 28 to 29, 1987, in the plots with nitrogen such as nitrogen alone, NP, NK, NPK, NPK + SiO$_2$ and NPK+compost plot etc. was lower than that in without nitrogen plots such as no fertilizer, compost alone, PK, P and K alone etc. and the amount of ethylene evolved from the leaf blades also showed the same trends. The correlation between the grain shattering rate and grain yield was negative but ethylene evolution was positively correlated with grain shattering rate of rice plant.ice plant.

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Quantitative parameters of primary roughness for describing the morphology of surface discontinuities at various scales

  • Belem, Tikou
    • Geomechanics and Engineering
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    • v.11 no.4
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    • pp.515-530
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    • 2016
  • In this paper, five different quantitative parameters were proposed for the characterization of the primary roughness which is the component of surface morphology that prevails during large strike-slip faults of more than 50 m. These parameters are mostly the anisotropic properties of rock surface morphology at various scales: (i) coefficient ($k_a$) and degree (${\delta}_a$) of apparent structural anisotropy of surface; (ii) coefficient ($k_r$) and degree (${\delta}_r$) of real structural anisotropy of surface; (iii) surface anisotropy function P(${\varphi}$); and (iv) degree of surface waviness ($W_s$). The coefficient and degree of apparent structural anisotropy allow qualifying the anisotropy/isotropy of a discontinuity according to a classification into four classes: anisotropic, moderately anisotropic/isotropic and isotropic. The coefficient and degree of real structural anisotropy of surface captures directly the actual surface anisotropy using geostatistical method. The anisotropy function predicts directional geometric properties of a surface of discontinuity from measurements in two orthogonal directions. These predicted data may subsequently be used to highlight the anisotropy/isotropy of the surface (radar plot). The degree of surface waviness allows qualifying the undulation of anisotropic surfaces. The proposed quantitative parameters allows their application at both lab and field scales.

Measurement of Hydrogenation Characteristics of Pd Film by Using Electrical Resistivity Measurement Technique (전기저항 측정법을 이용한 Pd박막의 수소화 특성 측정)

  • Cho, Young-Sin;Lee, Ho-Jae;Lee, Jong-Suk
    • Transactions of the Korean hydrogen and new energy society
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    • v.7 no.2
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    • pp.173-180
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    • 1996
  • 4-probe resistivity measurement technique was used to study kinetics of hydrogen absorption-desorption on Pd film($520{\AA}$ thick) at room temperature upto 1 bar. Kinetics data are fitted well to 1st order kinetics equation in ${\alpha}$ and ${\alpha}^{\prime}$ phases. In ${\alpha}+{\alpha}^{\prime}$ phase, absorption kinetics was very complicated, but it could be explained partially with nucleation and growth process. Ln(dR/dt) vs. time plot gives rate constant k value(R is resistance of sample, t is time). k value for absorption is $4^{-6}{\times}10^{-4}/sec$ in ${\alpha}$ phase. k is increasing upto $4^{\times}10^{-2}/sec$ as hydrogen pressure increasing in ${\alpha}^{\prime}$ phase. k is proportional to ln(Pop/Peq), where Peq is equilibrium plateau pressure and Pop is the opposing pressure. In contrast to bulk sample k value was decreasing with increasing number of A-D cycling in ${\alpha}^{\prime}$ phase absorption.

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Bioelectrical Impedance Analysis at Inner Forearms of the Human Body using Bioelectrical Impedance Measurement System

  • Kim, Jae-Hyung;Kim, Soo-Hong;Baik, Sung-Wan;Jeon, Gye-Rok
    • Journal of Korea Multimedia Society
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    • v.19 no.7
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    • pp.1146-1153
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    • 2016
  • The bioelectrical impedance (BI) at the inner forearms was measured using bioelectrical impedance measurement system (BIMS), which employs the multi-frequency and the two-electrode method. Experiments were performed as follows. First, while applying a constant alternating current of 800A to the inner region of the forearms, BI (Z) was measured at nineteen frequencies ranging from 5 to 500 kHz. The prediction marker (PM) was calculated for right and left forearm. The resistance (R) and the reactance (Xc) were simultaneously measured during impedance measurement. Second, a Cole-Cole plot (relationship between reactance and resistance) was obtained for left and right forearm, indicating the different characteristic frequencies (fc). Third, the phase angle was obtained, indicating strong dependence on the applied frequency.

Monitoring Characteristics of Protease Isolated from Squid Viscera (오징어 내장에서 분리한 Protease 특성의 모니터링)

  • 서지형;정용진;이기동;이명희
    • Journal of the East Asian Society of Dietary Life
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    • v.9 no.2
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    • pp.195-199
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    • 1999
  • The characteristics of protease from squid viscera was investigated by response surface methodology(RSM) programmed with reaction temperature and pH. The optimal temperature and pH for the protease were 41.75$^{\circ}C$ and pH 6.02 respectively. Also its activity was 78.65 unit at the optimal condition and $R^2$ of the model was 0.8461 (P<0.1). The protease activity was decreased by N $a^{+}$ and increased by $Mg^{2+}$ But $K^{+}$ did not affect the protease. The Km value against casein was determined to be 0.12 mM by Line-weaver-Burk plot.lot.

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Viscosity and Diffusion Constants Calculation of n-Alkanes by Molecular Dynamics Simulations

  • Lee, Song-Hi;Chang, Tai-Hyun
    • Bulletin of the Korean Chemical Society
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    • v.24 no.11
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    • pp.1590-1598
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    • 2003
  • In this paper we have presented the results for viscosity and self-diffusion constants of model systems for four liquid n-alkanes ($C_{12}, C_{20}, C_{32}, and C_{44}$) in a canonical ensemble at several temperatures using molecular dynamics (MD) simulations. The small chains of these n-alkanes are clearly $<{R_{ee}}^2>/6<{R_g}^2>>1$, which leads to the conclusion that the liquid n-alkanes over the whole temperatures considered are far away from the Rouse regime. Calculated viscosity ${\eta}$ and self-diffusion constants D are comparable with experimental results and the temperature dependence of both ${\eta}$ and D is suitably described by the Arrhenius plot. The behavior of both activation energies, $E_{\eta}$ and $E_D$, with increasing chain length indicates that the activation energies approach asymptotic values as n increases to the higher value, which is experimentally observed. Two calculated monomeric friction constants ${\zeta}$ and ${\zeta}_D$ give a correct qualitative trend: decrease with increasing temperature and increase with increasing chain length n. Comparison of the time auto-correlation functions of the end-to-end vector calculated from the Rouse model for n-dodecane ($C_{12}$) at 273 K and for n-tetratetracontane ($C_{44}$) at 473 K with those extracted directly from our MD simulations confirms that the short chain n-alkanes considered in this study are far away from the Rouse regime.

Studies on Biomass for Young Abies koreana Wilson

  • Lee, Do-Hyung;Yoon, Jun-Hyuck;Woo, Kwan-Soo
    • Journal of Korean Society of Forest Science
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    • v.96 no.2
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    • pp.138-144
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    • 2007
  • This study was undertaken to compare the biomass of Abies koreana growing at two sites. A $10{\times}10m$ plot was established in each site of a natural stand in Mt. Jiri and a plantation in Gyeongsan nursery. Five trees of A. koreana were randomly selected in each site. The following traits were investigated from each tree : height, basal diameter, age, weight of stem, branches, and needles as above-ground traits and weight of total roots, horizontal roots, and vertical roots as below-ground traits. In Gyeongsan nursery, age of sample trees was negatively correlated with both height and weight of total stem, while height was highly correlated with weight of horizontal roots. There was high correlation between the basal diameter and weight of total stem, and between the basal diameter and weight of roots. In Mt. Jiri stand, most of the above-ground traits except age were significantly correlated with the below-ground traits. The linear regression equation between the cross section area of base (X) and the weight of total stem (Y) in Gyeongsan nursery was Y=12.66X-12.92, and correlation was significant ($R^2=0.89$). The linear regression equation between the cross section area of base(X) and the weight of total branches (Y) in Mt. Jiri stand was Y=25.51X+6.00, and correlation was highly significant ($R^2=1.0$).