• Korean Journal of Ophthalmology
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• 제32권6호
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• pp.470-477
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• 2018

Purpose: To investigate the relationship between the progression of visual field (VF) loss and changes in lamina cribrosa depth (LCD) as determined by spectral-domain optical coherence tomography (SD-OCT) enhanced depth imaging in patients with primary open angle glaucoma (POAG). Methods: Data from 60 POAG patients (mean follow-up, $3.5{\pm}0.7$ years) were included in this retrospective study. The LCD was measured in the optic disc image using SD-OCT enhanced depth imaging scanning at each visit. Change in the LCD was considered to either 'increase' or 'decrease' when the differences between baseline and the latest two consecutive follow-up visits were greater than the corresponding reproducibility coefficient value ($23.08{\mu}m$, as determined in a preliminary reproducibility study). All participants were divided into three groups: increased LCD (ILCD), decreased LCD (DLCD), and no LCD change (NLCD). The Early Manifest Glaucoma Trial criteria were used to define VF deterioration. Kaplan-Meier survival analysis and Cox's proportional hazard models were performed to explore the relationship between VF progression and LCD change. Results: Of the 60 eyes examined, 35.0% (21 eyes), 28.3% (17 eyes), and 36.7% (22 eyes) were classified as the ILCD, DLCD, and NLCD groups, respectively. Kaplan-Meier survival analysis showed a greater cumulative probability of VF progression in the ILCD group than in the NLCD (p < 0.001) or DLCD groups (p = 0.018). Increased LCD was identified as the only risk factor for VF progression in the Cox proportional hazard models (hazard ratio, 1.008; 95% confidence interval, 1.000 to 1.015; p = 0.047). Conclusions: Increased LCD was associated with a greater possibility of VF progression. The quantitative measurement of LCD changes, determined by SD-OCT, is a potential biomarker for the prediction of VF deterioration in patients with POAG.

• 한국해양공학회지
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• 제35권2호
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• pp.141-149
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• 2021

The A60 class deck penetration piece is a fire-resistance apparatus installed on the deck compartment to protect lives and to prevent flame diffusion in the case of a fire accident in a ship or offshore plant. In this study, the sensitivity of the fire-resistance performance and approximation characteristics for the A60 class penetration piece was evaluated by conducting a transient heat-transfer analysis and fire test. The transient heat-transfer analysis was conducted to evaluate the fire-resistance design of the A60 class deck penetration piece, and the analysis results were verified via the fire test. The penetration-piece length, diameter, material type, and insulation density were used as the design factors (DFs), and the output responses were the weight, temperature, cost, and productivity. The quantitative effects of each DF on the output responses were evaluated using the design-of-experiments method. Additionally, an optimum design case was identified to minimize the weight of the A60 class deck penetration piece while satisfying the allowable limits of the output responses. According to the design-of-experiments results, various approximate models, e.g., a Kriging model, the response surface method, and a radial basis function-based neural network (RBFN), were generated. The design-of-experiments results were verified by the approximation results. It was concluded that among the approximate models, the RBFN was able to explore the design space of the A60 class deck penetration piece with the highest accuracy.

• 디지털융복합연구
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• 제17권11호
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• pp.149-156
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• 2019

정보기술의 활용이 보편화되면서 대중과 연구자 모두 정보 프라이버시 문제에 대한 관심이 높아지고 있다. 이러한 문제에 대한 연구가 기하급수적으로 증가하면서 연구결과에 대한 전반적인 이해가 어려워졌다. 이에 따라 과거연구에 대한 체계적인 검토가 요구된다. 본 연구는 정보 프라이버시 연구에 핵심적인 네 가지 연구개념을 두 가지 연구모형으로 설정하고 기존 연구에서 수집된 데이터를 이용하여 실증 분석하였다. TSSEM이라는 정량적 메타분석 기법이 적용되었는데, 이 기법은 MASEM의 한 가지로서 구조방정식모형과 메타분석 기법을 통합하여 분석하는 기능을 제공한다. 분석결과는 위험 중심적 모형이 염려 중심적 모형과 비교하여 보다 높은 모형 적합도를 나타내었다. 본 연구의 결과는 전통적인 염려 중심적 모형의 설명력에 의문을 제시하며, 사용자의 프라이버시 정보 제공의도를 설명하기 위하여 위험 중심적 모형을 고려할 필요가 있다는 점을 시사한다.

• Journal of Veterinary Science
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• 제19권6호
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• pp.735-743
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• 2018

We investigated the effect of transforming growth factor beta 1 ($TGF-{\beta}1$) on equine hyaluronan synthase 2 (HAS2) gene expression and hyaluronan (HA) synthesis in culture models of articular chondrocytes. Equine chondrocytes were treated with $TGF-{\beta}1$ at different concentrations and times in monolayer cultures. In three-dimensional cultures, chondrocyte-seeded gelatin scaffolds were cultured in chondrogenic media containing 10 ng/mL of $TGF-{\beta}1$. The amounts of HA in conditioned media and in scaffolds were determined by enzyme-linked immunosorbent assays. HAS2 mRNA expression was analyzed by semi-quantitative reverse transcription polymerase chain reaction. The uronic acid content and DNA content of the scaffolds were measured by using colorimetric and Hoechst 33258 assays, respectively. Cell proliferation was evaluated by using the alamarBlue assay. Scanning electron microscopy (SEM), histology, and immunohistochemistry were used for microscopic analysis of the samples. The upregulation of HAS2 mRNA levels by $TGF-{\beta}1$ stimulation was dose and time dependent. $TGF-{\beta}1$ was shown to enhance HA and uronic acid content in the scaffolds. Cell proliferation and DNA content were significantly lower in $TGF-{\beta}1$ treatments. SEM and histological results revealed the formation of a cartilaginous-like extracellular matrix in the $TGF-{\beta}1$-treated scaffolds. Together, our results suggest that $TGF-{\beta}1$ has a stimulatory effect on equine chondrocytes, enhancing HA synthesis and promoting cartilage matrix generation.

• International Journal of Computer Science & Network Security
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• 제22권6호
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• pp.346-356
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• 2022

The jurisprudential legal rules govern the way Muslims react and interact to daily life. This creates a huge stream of questions, that require highly qualified and well-educated individuals, called Muftis. With Muslims representing almost 25% of the planet population, and the scarcity of qualified Muftis, this creates a demand supply problem calling for Automation solutions. This motivates the application of Artificial Intelligence (AI) to solve this problem, which requires a well-designed Question-Answering (QA) system to solve it. In this work, we propose a QA system, based on retrieval augmented generative transformer model for jurisprudential legal question. The main idea in the proposed architecture is the leverage of both state-of-the art transformer models, and the existing knowledge base of legal sources and question-answers. With the sensitivity of the domain in mind, due to its importance in Muslims daily lives, our design balances between exploitation of knowledge bases, and exploration provided by the generative transformer models. We collect a custom data set of 850,000 entries, that includes the question, answer, and category of the question. Our evaluation methodology is based on both quantitative and qualitative methods. We use metrics like BERTScore and METEOR to evaluate the precision and recall of the system. We also provide many qualitative results that show the quality of the generated answers, and how relevant they are to the asked questions.

• The Korean Journal of Physiology and Pharmacology
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• 제27권3호
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• pp.197-208
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• 2023

Dysregulation of certain long non-coding RNAs may facilitate tumor initiation and progression. However, numerous carcinogenesis-related long noncoding RNAs have not been characterized. The goal of this study was to elucidate the role of LINC00562 in gastric cancer (GC). The expression of LINC00562 was analyzed using real-time quantitative PCR and Western blotting. The proliferative capacity of GC cells was determined using Cell Counting Kit-8 and colony-formation assays. The migration of GC cells were evaluated using wound-healing assays. The apoptosis of GC cells was assessed by measuring the expression levels of apoptosis-related proteins (Bax and Bcl-2). Xenograft models in nude mice were constructed for in vivo functional analysis of LINC00562. The binding relationship between miR-4636 and LINC00562 or adaptor protein complex 1 sigma 3 (AP1S3), obtained from public databases, was confirmed using dual-luciferase and RNA-binding protein immunoprecipitation experiments. LINC00562 was expressed in GC cells at high levels. Knockdown of LINC00562 repressed GC cell growth and migration, promoted apoptosis in vitro, and inhibited tumor growth in nude mouse models. LINC00562 directly targeted miR-4636, and miR-4636 depletion restored the GC cell behavior inhibited by LINC00562 absence. AP1S3, an oncogene, binds to miR-4636. MiR-4636 downregulation increased AP1S3 level, restoring GC cell malignant behaviors inhibited by AP1S3 downregulation. Thus, LINC00562 exerts carcinogenic effects on GC development by targeting miR-4636-mediated AP1S3 signaling.

• 스마트미디어저널
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• 제12권7호
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• pp.76-83
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• 2023

본 연구에서는 웹툰 작가의 웹툰 제작 과정을 보조하기 위해 사전학습된 Text-to-Image 모델을 미세조정하여 텍스트에서 웹툰을 생성하는 방법을 제안한다. 제안하는 방법은 웹툰 화풍으로 변환된 웹툰 데이터셋을 사용하여 사전학습된 Stable Diffusion 모델에 LoRA 기법을 활용하여 미세조정한다. 실험 결과 3만 스텝의 학습으로 약 4시간 반이 소요되어 빠르게 학습하는 것을 확인하였으며, 생성된 이미지에서는 입력한 텍스트에 표현된 형상이나 배경이 반영되어 웹툰 이미지가 생성되는 것을 확인하였다. 또한, Inception score를 통해 정량적인 평가를 수행하였을 때, DCGAN 기반의 Text-to-Image 모델보다 더 높은 성능을 나타냈다. 본 연구에서 제안된 웹툰 생성을 위한 Text-to-Image 모델을 웹툰 작가가 사용한다면, 웹툰 저작에 시간을 단축시킬 수 있을 것으로 기대된다.

• Journal of Information Science Theory and Practice
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• 제11권2호
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• pp.82-103
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• 2023

The Zimbabwean healthcare sector faces huge challenges due to increased demands for improved services for a growing number of patients with fewer resources. The use of information and communications technologies, prevalent in many industries, but lacking in Zimbabwean healthcare, could increase productivity and innovation. The adoption of health management information systems (HMISs) can lead to improved patient safety and high-level patient care. These technologies can change delivery methods to be more patient focused by utilising integrated models and allowing for a continuum of care across healthcare providers. However, implementation of these technologies in the health care sector remains low. The purpose of this study is to demonstrate the advantages to be attained by using HMISs in healthcare delivery and to ascertain the factors that influence the uptake of such systems in the public healthcare sector. A conceptual model, extending the technology, organization, and environment framework by means of other adoption models, underpins the study of adoption behavior. A mixed method methodology was used to conduct the study. For the quantitative approach, questionnaires were used to allow for regression analysis. For the qualitative approach, thematic analysis was used to analyse interview data. The results showed that the critical success factors (namely, relative advantage, availability, complexity, compatibility, trialability, observability, management support, information and communication technology expertise, communication processes, government regulation, infrastructure support, organizational readiness, industry and competitive support, external support, perceived ease of use, perceived usefulness, attitude, and intention to use) influenced adoption of HMISs in public hospitals in Zimbabwe.

• Journal of Veterinary Science
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• 제24권4호
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• pp.53.1-53.12
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• 2023

Background: Mammalian orthoreovirus type 3 (MRV3), which is responsible for gastroenteritis in many mammalian species including pigs, has been isolated from piglets with severe diarrhea. However, the use of pig-derived cells as an infection model for swine-MRV3 has rarely been studied. Objectives: This study aims to establish porcine intestinal organoids (PIOs) and examine their susceptibility as an in vitro model for intestinal MRV3 infection. Methods: PIOs were isolated and established from the jejunum of a miniature pig. Established PIOs were characterized using polymerase chain reaction (PCR) and immunofluorescence assays (IFAs) to confirm the expression of small intestine-specific genes and proteins, such as Lgr5, LYZI, Mucin-2, ChgA, and Villin. The monolayered PIOs and three-dimensional (3D) PIOs, obtained through their distribution to expose the apical surface, were infected with MRV3 for 2 h, washed with Dulbecco's phosphate-buffered saline, and observed. Viral infection was confirmed using PCR and IFA. We performed quantitative real-time reverse transcription-PCR to assess changes in viral copy numbers and gene expressions linked to intestinal epithelial genes and antiviral activity. Results: The established PIOs have molecular characteristics of intestinal organoids. Infected PIOs showed delayed proliferation with disruption of structures. In addition, infection with MRV3 altered the gene expression linked to intestinal epithelial cells and antiviral activity, and these effects were observed in both 2D and 3D models. Furthermore, viral copy numbers in the supernatant of both models increased in a time-dependent manner. Conclusions: We suggest that PIOs can be an in vitro model to study the infection mechanism of MRV3 in detail, facilitating pharmaceutical development.

• 한국터널지하공간학회 논문집
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• 제25권6호
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• pp.447-458
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• 2023

본 연구에서는 쉴드터널 세그먼트 라이닝의 하중과 부재저항의 확률적 특성뿐만 아니라 경계조건의 변동성을 고려한 비교모델을 선정하고 신뢰성해석을 수행하였으며, 파괴확률 산정 및 구조안전성 검토를 통해 한계상태설계의 적정성에 대해 분석을 수행하였다. 이러한 지반 정수의 확률특성치를 고려한 해석을 위해 지반스프링계수는 Muirwood식을 적용하여 정량적 값을 산정하여 Mean값으로 고려하였고, 변동계수는 기존 연구자료를 토대로 지반 경계조건 변화에 따른 검토대상 모델들을 선정하였다. 이러한 모델들에 대한 구조해석과 MCS기법을 적용한 신뢰성분석을 통해 파괴확률과 신뢰성지수를 산정하여 지반경계조건 변화에 따른 파괴확률의 변화를 검토하였다.