• Entomological Research
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• v.48 no.5
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• pp.390-399
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• 2018

In order to precisely assess gene expression levels, the suitable internal reference genes must be served to quantify real-time reverse transcription polymerase chain reaction (RT-qPCR) data. For armyworm, Mythimna separata, which reference genes are suitable for assessing the level of transcriptional expression of target genes have yet to be explored. In this study, eight common reference genes, including ${\beta}$-actin (${\beta}$-ACT), 18 s ribosomal (18S), 28S ribosomal (28S), glyceraldehyde-3-phosphate (GAPDH), elongation fator-alpha ($EF1{\alpha}$), TATA box binding protein (TBP), ribosomal protein L7 (RPL7), and alpha-tubulin (${\alpha}$-TUB) that in different developmental stages, tissues and insecticide treatments of M. separata were evaluated. To further explore whether these genes were suitable to serve as endogenous controls, three software-based approaches (geNorm, BestKeeper, and NormFinder), the delta Ct method, and one web-based comprehensive tool (RefFinder) were employed to analyze and rank the tested genes. The optimal number of reference genes was determined using the geNorm program, and the suitability of particular reference genes was empirically validated according to normalized HSP70, and MsepCYP321A10 gene expression data. We found that the most suitable reference genes for the different experimental conditions. For developmental stages, 28S/RPL7 were the optimal reference genes, both $RPL7/EF1{\alpha}$ were suitable for experiments of different tissues, whereas for insecticide treatments, $28S/{\alpha}-TUB$ were suitable for normalizations of expression data. In addition, $28S/{\alpha}-TUB$ were the suitable reference genes because they have the most stable expression among different developmental stages, tissues and insecticide treatments. Our work is the first report on reference gene selection in M. separata, and might serve as a precedent for future gene expression studies.

• Journal of Korea Water Resources Association
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• v.53 no.12
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• pp.1097-1107
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• 2020

A methodology has been proposed to understand the spatiotemporal changes of the river topography through the longitudinal change of the geometric characteristics of the cross-sections and the properties related thereto. Three-dimensional spatial information of the riverbed was obtained through the detailed bathymetry survey using an acoustic echo sounder for the reach from Gumi Weir to Chilgok Weir in the Nakdong river. Geometric informations for the reference sections were extracted using the acquired bathymetry survey data. By comparing the geometric properties for the reference sections, it was possible to catch the topographic characteristics and its changes over a reach of the channel. Through comparison with past survey data, it was also possible to quantitatively grasp the amount of change in cross-sectional area and volumetric change of riverbed. It is expected that a quantitative evaluation of river topography changes will be possible by applying the method proposed in this study.

• Journal of Ocean Engineering and Technology
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• v.36 no.1
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• pp.32-40
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• 2022

Underwater optical images face various limitations that degrade the image quality compared with optical images taken in our atmosphere. Attenuation according to the wavelength of light and reflection by very small floating objects cause low contrast, blurry clarity, and color degradation in underwater images. We constructed an image data of the Korean sea and enhanced it by learning the characteristics of underwater images using the deep learning techniques of CycleGAN (cycle-consistent adversarial network), UGAN (underwater GAN), FUnIE-GAN (fast underwater image enhancement GAN). In addition, the underwater optical image was enhanced using the image processing technique of Image Fusion. For a quantitative performance comparison, UIQM (underwater image quality measure), which evaluates the performance of the enhancement in terms of colorfulness, sharpness, and contrast, and UCIQE (underwater color image quality evaluation), which evaluates the performance in terms of chroma, luminance, and saturation were calculated. For 100 underwater images taken in Korean seas, the average UIQMs of CycleGAN, UGAN, and FUnIE-GAN were 3.91, 3.42, and 2.66, respectively, and the average UCIQEs were measured to be 29.9, 26.77, and 22.88, respectively. The average UIQM and UCIQE of Image Fusion were 3.63 and 23.59, respectively. CycleGAN and UGAN qualitatively and quantitatively improved the image quality in various underwater environments, and FUnIE-GAN had performance differences depending on the underwater environment. Image Fusion showed good performance in terms of color correction and sharpness enhancement. It is expected that this method can be used for monitoring underwater works and the autonomous operation of unmanned vehicles by improving the visibility of underwater situations more accurately.

• Journal of Digital Policy
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• v.1 no.2
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• pp.79-87
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• 2022

This study is a participatory action research conducted to develop a program to prevent musculoskeletal disorders among kitchen workers in a general hospital. This study was conducted from March to December 2018 with 15 kitchen workers, 3 nutritionists, 2 nurses, and 1 physical therapist working at C general hospital located in G city. Based on the conceptual model of participatory practice research, quantitative and qualitative data were collected and analyzed comprehensively. As a result of developing a program for preventing musculoskeletal disorders and applying it 13 times, the satisfaction level was high in the composition, operation time, and expertise of the program, and it was found that they wanted to operate the program continuously in the future. Therefore, based on the results of this study, it is necessary to develop and apply a musculoskeletal disease prevention program that considers the characteristics of the work department and work targeting other workers through participatory action research.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.16 no.8
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• pp.2831-2845
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• 2022

Compared to the significant approach of project-based learning research, a data-driven design project-based learning has not reached a meaningful consensus regarding the most valid and reliable method for assessing design creativity. This article proposes an advanced information data-interactive learning system for creative design using a service design process that combines a design thinking. We propose a service framework to improve the convergence design process between students and advanced information data analysis, allowing students to participate actively in the data visualization and research using patent data. Solving a design problem by discovery and interpretation process, the Advanced information-interactive learning framework allows the students to verify the creative idea values or to ideate new factors and the associated various feasible solutions. The student can perform the patent data according to a business intelligence platform. Most of the new ideas for solving design projects are evaluated through complete patent data analysis and visualization in the beginning of the service design process. In this article, we propose to adapt advanced information data to educate the service design process, allowing the students to evaluate their own idea and define the problems iteratively until satisfaction. Quantitative evaluation results have shown that the advanced information data-driven learning system approach can improve the design project - based learning results in terms of design creativity. Our findings can contribute to data-driven project-based learning for advanced information data that play a crucial role in convergence design in related standards and other smart educational fields that are linked.

• Korean Journal of Veterinary Service
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• v.45 no.2
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• pp.87-99
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• 2022

A novel porcine circovirus 4 (PCV4) was recently emerged in Chinese and Korean pig herds, which provided epidemiological situation where three pathogenic PCVs, PCV2, PCV3, and newly emerged PCV4, could co-infect pig herds in these countries. In this study, a new triplex quantitative real-time polymerase chain reaction (tqPCR) method was developed for the rapid and differential detection of these viruses. The assay specifically amplified each viral capsid gene, whereas no other porcine pathogenic genes were detected. The detection limit of the assay was below 10 copies/µL and the assay showed high repeatability and reproducibility. In the clinical evaluation using 1476 clinical samples from 198 Korean pig farms, the detection rates of PCV2, PCV3 and PCV4 by the tqPCR assay were 13.8%, 25.4%, and 3.8%, respectively, which were 100% agreement with those of previously reported monoplex qPCR assays for PCV2, PCV3, and PCV4, with a κ value (95% CI) of 1 (1.00~1.00). The prevalence of PCV2, PCV3, and PCV4 at the farm levels were 46.5%, 63.6%, and 19.7%, respectively. The co-infection analysis for tested pig farms showed that single infection rates for PCV2, PCV3, and PCV4 were 28.8%, 44.4%, and 9.6%, respectively, the dual infection rates of PCV2 and PCV3, PCV2 and PCV4, and PCV3 and PCV4 were 12.6%, 3.5%, and 5.1%, respectively, and the triple infection rate for PCV2, PCV3, and PCV4 was 1.5%. These results demonstrate that three pathogenic PCVs are widely spread, and their co-infections are common in Korean pig herds, and the newly developed tqPCR assay will be useful for etiological and epidemiological studies of these pathogenic PCVs.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.241-241
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• 2023

최근 수십 년 동안 전 세계의 거의 모든 하천 유역은 기후변화와 인간 활동에 의해 직간접적으로 영향을 받았다. 특히, 유역 유출량은 기후변화와 인간 활동의 상호작용으로 수자원 분야에서 문제가 되고 있다. 기후와 인간 활동에 의해 변화하는 환경에서 수자원을 효율적으로 관리하기 위해서 유출량의 변화를 이해하는 것은 매우 중요하다. 따라서, 본 연구에서는 낙동강 유역의 22개 중권역을 대상으로 과거 관측자료 및 미래 기후변화 시나리오의 유출량, 강수량, 기온, 잠재 증발산량 및 실제 증발산량 자료를 이용하여 기후변화와 인간 활동이 유출량에 미치는 영향을 정량적으로 분석하였다. 과거 관측자료를 분석할 경우, 기초자료인 유출량의 경향성 및 유의성을 검증하기 위해 비모수적 방법인 Mann-Kendall 검증을 수행하였으며, Pettitt method를 이용하여 변화 지점을 결정하여 기준기간과 사후기간으로 구분하였다. 또한 Budyko 가설 기반 기후 탄력성 접근법을 이용하여 기후변화와 인간 활동이 하천 유출량에 미치는 영향을 정량적으로 분리하였다. 미래 RCP 시나리오 자료를 분석할 경우에도 기간을 나누고 기후 탄력성 접근법을 이용하여 유출량의 영향을 평가하였다. 분석 결과, 기후변화와 인간 활동의 상대적 기여도는 중권역 간에도 다양하다는 것을 확인할 수 있었다. 미래의 유출량을 분석한 결과, 대부분의 유역에서 기후변화의 기여도는 RCP 4.5일 때에 비해 RCP 8.5에서 더 크게 상승한 것으로 나타났다. 과거 기간(1966~2020)에 대하여 미래 기간 (2062~2099)에 대한 분석에서 중권역 중 금호강(#2012)은 RCP 4.5에서는 22.4%, RCP 8.5에서는 39.8%로 RCP 8.5가 큰 것으로 나타났다.

• Journal of the Korean Society for Precision Engineering
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• v.12 no.9
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• pp.74-85
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• 1995

The purpose of this paper is to evaluate the grade of WA vitrified and resinoid bond grinding wheels by the sue of AE measuring system. When the manufactured 48 kinds of specimens were scratched by the method of OKOSHI'S grade test, the relationship between the amount of bit scratch depth of grinding wheel specimens and the character- istics of AE signals, and the relationship of AE counts and grade were considered as fololws; (1) The higher the grades are AE cumlulative event counts N and AE event count rate n, the smaller the values tend to be. But A $E_{rms}$is in reverse. (2) In the case of same grade, the smaller the grain size is, the higher the value of AE cumulative event counts N and A $E_{rms}$is results of comparison and observation. The grinding wheel with lower elasticity and with higher percentage of pore detected higher value of AE cumula- tive event counts N than with higher elasticity and lower percentage of pore. But A $E_{rms}$ is in reverse. (3) AE cumulative event counts N and bit scratch depth h have normally one to one correspondence. (4) It can be expected that quantitative evaluations of grade by using AE have been carried out by the wave observation of AE signal in line with the relationship between load speed of bit and AE cumulative event counts N & AE event count rate n.' AE event count rate n.ate n.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2021.05a
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• pp.52-54
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• 2021

With the advent of the era of artificial intelligence, robot education and teaching under its empowerment have been widely concerned and applied worldwide. The purpose of this study: systematically evaluate the application effect of AI educational robots in student education and teaching; the method of this study: use the computer to search for relevant education in the search tools such as "Web of Science", "CNKI", "ERIC", "IEEE" A comparative study of the effects of robot teaching and traditional teaching. The retrieval time is from January 2000 to January 2020. Comprehensive MetaAnalysis 2.0 was used for Meta analysis. The results of this study: A quantitative analysis of the 31 valid research literatures included, and an objective evaluation of the effect of the meta-analysis on AI educational robots. The analysis results show that the combined effect of AI educational robots on student learning effects is 0.465 This indicates that educational robots have a moderately positive effect on students 'learning effectiveness. The conclusion of this study: The application effect of AI educational robots in student education and teaching is better than traditional education methods, which can better promote student learning.

• Journal of the Korea Fashion and Costume Design Association
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• v.25 no.2
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• pp.1-16
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• 2023

Due to the current seriousness of environmental pollution and the eco-friendly movement of the fashion industry, research on sustainable fashion design is being actively conducted. In this study, consumer perception of upcycling products, are divided into image, function, and meaning; and image is further divided into shape, color, and material. It was redefined as pattern, and image recognition was evaluated among men and women in their 20s and 30s, and men and women in their 40s and 50s used as subjects. First, factors that determine each image were extracted based on qualitative analysis of the precedent cases of upcycling bags, and quantitative analysis of the subjects was induced through a questionnaire. As a result of the analysis of evaluation items related to image association, the average frequency analysis of all subjects for each stimuli and the cognitive variance of the frequency analysis by generation by gender were found to be similar. However, awareness of some stimuli by generation showed a significant difference. Overall, in the three stimuli with high overall preference, common features, such as the basic box-shaped symmetrical structure, the monochromatic color of the Munsell system, solid and practical texture, and appropriate use of patterns were identified. In addition, it was confirmed that there was a difference with factors such as femininity, simplicity, touch, and splendor in the measurement factors. In conclusion, it is considered that the main significance of this study is that it excluded the recognition and meaning of upcycling products and explored the original design and image elements of products. Therefore, it is expected that this study will be used as a basic data for responding to the gender image of each generation as an alternative method of sustainable fashion design, and it will be an opportunity to expand the scope of the study to a detailed study beyond the biased topic.