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A retrospective study of single frozen-thawed blastocyst transfer

  • Hur, Yong Soo;Ryu, Eun Kyung;Song, Seung Hyun;Yoon, San Hyun;Lim, Kyung Sil;Lee, Won Don;Lim, Jin Ho
    • Clinical and Experimental Reproductive Medicine
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    • v.43 no.2
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    • pp.106-111
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    • 2016
  • Objective: To study the clinical outcomes of single frozen-thawed blastocyst transfer cycles according to the hatching status of frozen-thawed blastocysts. Methods: Frozen-thawed blastocysts were divided into three groups according to their hatching status as follows: less-than-expanded blastocyst (${\leq}EdB$), hatching blastocyst (HgB), and hatched blastocyst (HdB). The female age and infertility factors of each group were evaluated. The quality of the single frozen-thawed blastocyst was also graded as grade A, tightly packed inner cell mass (ICM) and many cells organized in the trophectoderm epithelium (TE); grade B, several and loose ICM and TE; and grade C, very few ICM and a few cells in the TE. The clinical pregnancy and implantation rate were compared between each group. The data were analyzed by either t-test or chi-square analysis. Results: There were no statistically significant differences in average female ages, infertility factors, or the distribution of blastocyst grades A, B, and C in each group. There was no significant difference in the clinical pregnancy and implantation rate of each group according to their blastocyst grade. However, there was a significant difference in the clinical pregnancy and implantation rate between each group. In the HdB group, the clinical pregnancy and implantation rate were similar regardless of the blastocyst quality. Conclusion: There was an effect on the clinical outcomes depending on whether the blastocyst hatched during single frozen-thawed blastocyst transfer. When performing single frozen-thawed blastocyst transfer, the hatching status of the frozen-thawed blastocyst may be a more important parameter for clinical outcomes than the quality of the frozen-thawed blastocyst.

Analysis of Carcass Quality Grade Components and ChemicoPhysical and Sensory Traits of M. longissimus dorsi in Hanwoo (한우 도체 육질등급 요인 분석과 육질등급에 따른 이화학 및 관능 특성)

  • Lee, J.M.;Park, B.Y.;Cho, S.H.;Kim, J.H.;Yoo, Y.M.;Chae, H.S.;Choi, Y.I.
    • Journal of Animal Science and Technology
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    • v.46 no.5
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    • pp.833-840
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    • 2004
  • The current study was conducted to analysis the effects of sex and live weight on carcass characteristics using total 773 Hanwoo, and to assess the effects of quality grade on chemico-physical and sensory characteristics in longissimus muscle. Results show that both steer and cow received superior quality grade to bull within the same weight c1ass(P < 0.05). Marbling score, texture, maturity, meat color and fat color were significantly( P< 0.01) affected by sex, whilst only marbling score and maturity were significantly affected by live weight. The ultimate quality grade showed significant relationships with marbling score(r = - $0.81^{**}) meat color (r= 0.21^{**}) fat color(r = 0.10^{**}) and with texture(r = 0.41^{**})$ but that had no such a relationship with maturity. WB-shear force and cooking loss were decreased when carcass grade was increased(P < 0.01), but water-holding capacity was identical between the quality grades. Objective meat color dimensions in lightness, redness and yellowness were increased for higher quality grade(P< 0.01). Meat flavor, juiciness and tenderness received higher scores for higher quality grade(P< 0.01). Intramuscular fat content had significantly relationships with WB-shear force(r = 0.$56^*), water-holding capacity(r = 0.18^{**}), juiciness(r = 0.46^{**}), tenderness(r= 0.49^{**})$, and flavor intensity(r = 0.$34^*$). In addition, a higher WB-shear force was related to a lower flavor, tenderness and juiciness scores.

Altitudes or EMT and Nursing students toward Euthanasia (일부 응급구조과 학생과 간호과 학생의 안락사에 대한 태도조사 연구)

  • Kim, Mi-Seon;Park, Mi-Hwa;Hwang, Seon-Young
    • The Korean Journal of Emergency Medical Services
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    • v.6 no.1
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    • pp.39-53
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    • 2002
  • This descriptive study was conducted to identify the attitude toward euthanasia of EMT and nursing students and to compare their attitude before and after clinical practice. The convenience sample was comprised of 40 first grade and 40 second grade EMT students attended at G college in G-city, and 40 first grade and 40 third grade nursing students attended at C college in C-province. The variable was tested with an euthanasia attitude scale developed by Kim Ae Kyoung(2001). The valid responses were obtained and analyzed by using SPSS PC+ from November 25 to December 6, 2002. The results showed that most of the respondents agreed to have positive attitude toward euthanasia in terms of client's right and client's quality of life. Also, they thought that euthanasia should be legalized and the right to die with dignity should be guaranteed. The EMT respondents having a clinical experience showed significantly higher score than respondents having no experience in the subarea of medical ethics, and nursing respondents who had a clinical practice showed significantly higher score than others no experience in the subarea of quality of life. But, the other sub-areas had no significant difference between the respondents before and after clinical practice. The attitude toward euthanasia composed of sub-areas such as being after the quality of life, having a high regard for a life and recognizing client's right was not affected by the single factor of clinical practice. Therefore, much supportive intervention need to be done for the students about the significant affecting factors found in this study like a religion, values, education and observing experience of someone's death.

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Development of Autometic Tomato Grade System with Using a Color Image Processing (칼라 영상을 이용한 토마토 자동 선별시스템의 개발)

  • 이지훈;최연호;김우현;윤경섭;권우현
    • Proceedings of the IEEK Conference
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    • 2003.07c
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    • pp.2529-2532
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    • 2003
  • The quality of agricultural products is represented a degree of freshness and a special qualify that has a close relation to commercial value. To grade tomatoes, it used to nondestructive equipment of a charge-coupled device(CCD) camera and near-infrared(NIR) spectrum analysis method. The NIR spectrum analysis method is used to determine internal qualities such as a brix and an acidity. The CCD camera is used to measure external qualities like color and a size of tomatoes. This paper explaines the structure and movement of the automatic grade system and applies the algorithm for deformed tomtatoes and characteristics of tomatoes through image processing to the grade system.

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Color Appearances and Morphological Characteristics of Rice According to the Visual Acceptance (외관 기호도에 의한 쌀의 색택 및 형태관련 특성)

  • Song, Jin;Chun, A-Reum;Kim, Sun-Lim;Kim, Deog-Su;Son, Jong-Rok
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.7
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    • pp.618-623
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    • 2006
  • This study was carried out to select the acceptance test influenced by some quality characteristics of rice, and may provide the basic information on rice grade of appearance quality as distinguished the numerical values. Forty-four Japonica rice varieties mainly cultivated in Korea were evaluated for a consumer acceptance test. Color preferences of rice were highly correlated with appearance quality (r=-0.897**) and redness (r=-0.893**). Especially, appearance quality value resulted from interaction of $redness{\times}yellowness$ values was expected as a specific character used for grade of rice appearance. Shape preferences of rice showed the positive correlation with grain width (r=0.527**) and grain size (r=0.454**). Result of the consumer acceptance test of rice appearance conducted through a cluster analysis revealed five groups. Our study suggests that it may be feasible to be graded by average of quality character among groups, grain width, and grain size in Duncan's multiple range test.

Effects of Addition of Perilla Leaf Powder and Carcass Grade on the Quality and Palatability of Pork Sausage (깻잎 분말 첨가와 도체등급이 돈육소시지의 품질 및 기호성에 미치는 영향)

  • 정인철;강세주;김종기;현재석;김미숙;문윤희
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.3
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    • pp.350-355
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    • 2003
  • This study was carried out to clarify the effect of addition of perilla leaf powder (PLP) and carcass grade on the quality and palatability of pork sausage. The chemical composition, pH, calorie, water holding capacity, surface color, textural properties and amino acid composition of the samples were determined, and sensory characteristics were evaluated. Moisture and crude ash were not different among sausage of four type. Crude fat of grade B sausage was higher than that of grade E sausage, and grade B sausage added PLP was higher than grade B sausage without PLP. Crude Protein of grade E sausage was higher than that of grade B sausage. The pH of grade E sausage added PLP was highest, and calorie of grade B sausage without PLP was highest among sausage of four type. Water holding capacity of grade B added and free PLP was significantly higher than grade I sausage. The residual nitrite of sausage added PLP was significantly lower than sausage without PLP. In case of Hunter's $L^{*}$, grade B sausage and free PLP sausage were significantly higher than grade E sausage and sausage added PLP, respectively. In case of Hunter' $a^{*}$, grade E sausage and free PLP sausage were significantly higher than grade B sausage and sausage added PLP. Hunter's $b^{*}$ of sausage added PLP was higher than that of free PLP sausage. Textural properties, hardness, springiness, cohesiveness and chewiness were significantly different among sausage of four type, but gumminess of grade E added PLP was lowest among sausage of four type. Total amino acid was not significantly different among sausage of four type. Sensory color and texture of grade B sausage were superior to grade I sausage, palatability of free PLP sausage was superior.sage was superior.

The Relationship Between Carcass Color Grade and Instrumental Values in Pork Loin (돼지도체 등급판정기준 육색등급과 기계적 측정값간의 상관관계)

  • Kim, Dong-Jun;Lee, Do-Heon;Lee, Yong-Gi;Park, Dong-Woen;Kim, Gap-Don;Jung, Eun-Young;Seo, Hyun-Woo;Jeong, Jin-Yeon;Joo, Seon-Tea;Yang, Han-Sul
    • Journal of agriculture & life science
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    • v.46 no.1
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    • pp.133-139
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    • 2012
  • The objective of this study was to investigate the relationship between color grade in Korea Institute for Animal Products Quality Evaluation (KAPE) and instrumental color values ($L^*$, $a^*$, $b^*$, ${\Delta}C$ and H) in porcine longissimus dorsi muscle. A total of 538 pigs were selected to classify and to measure the instrumental color values from commercial public slaughter house. All of the instrumental color traits were decreased with increase of color grade (p<0.05). The color grade 1 group had the highest $L^*$ value (65.23) and the color grade 6 group had the lowest $L^*$ value (45.29) among the color grade groups (p<0.05). The color grade had the negative correlation with all the instrumental color traits (p<0.001) and the correlation coefficient between color grade and $L^*$ value was -0.83. In conclusion, instrumental color traits is highly related to color grade, and especially $L^*$ value is an important indicator for both porcine carcass grading and color grading systems in KAPE.

A Fundamental Study on Manufacturing Condition the High Quality Recycled Fine Aggregate by Low Speed Wet Rotary Mill (저속습식마쇄기를 이용한 고품질 순환잔골재 제조에 관한 기초적 연구)

  • Kim, Ha-Seog;Lee, Gyung-Hyun;Ra, Jeong-Min;Park, Hyo-Jin;Lim, Dae-Bin;Kim, Jin-Man
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2009.11a
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    • pp.65-68
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    • 2009
  • Recycled aggregate by the recycling construction waste has a lot of advantage such as the developing the alternative resource and protecting of environment. However, recycled aggregate is used as the low quality grade, because the technic to remove old mortar from aggregate is low level. To use the recycled aggregate as high quality grade, it is important to develop the technic to produce the high quality recycled aggregate. To manufacture the high quality recycled aggregate, old mortar attached on the aggregates should remove efficiently. Therefore, in this study, we suggested the optimum condition to remove old mortar effectively using sulfuric acid and low speed wet rotary mill for high quality recycled fine aggregate. The results shows that the recycled aggregate satisfy on the standards of KS F 2573 in density, absorption and solid volume when, adequate condition of sulfuric mole ratio and aggregate ratio are make.

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Effectiveness of Creative Drama in Promoting Social Competence in Peer Abused/At-Risk Children (교육연극을 적용한 사회적 유능성 중재 프로그램의 집단따돌림/위험 아동의 사회적 유능성 증진 효과 연구)

  • Chung, Kai Sook
    • Korean Journal of Child Studies
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    • v.23 no.4
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    • pp.171-183
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    • 2002
  • This study examined the applicability of creative drama to a social competence program for poorly accepted(socially at-risk) elementary school children. The subjects were 40 $2^{nd}$(n=20) and $5^{th}$(n=20) grade children. The experimental group(n=20) participated in a social competence program that included 18 sessions of the creative drama activities during winter vacation. The Peer Rating Scale, the Peer Competence Scale, and the Quality of Friendship Scale were administered as pre- and post-test. Covariance analyses showed no differences between experimental and control groups on peer competence and quality of friendship, but the children who participated in the program showed some differences in total scores and sub-areas on quality of friendship by grade and level of peer pre-acceptance.

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Quality improvement and aging effect of beef by low-temperature treatment of non-preferred parts of beef (비선호 부위 소고기의 저온처리에 의한 품질향상 및 소고기의 숙성효과)

  • Hyun Kyoung Kim;Soon Cheol Kim;Hyeon Jin Kim;Yeong Mi Kim
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.5
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    • pp.753-760
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    • 2023
  • In this study, quality improvement of beef was attempted according to the low temperature treatment and aging period of grade 1 compared to grade 1++ beef. The fat content and shear force of beef grade 1++ were 13.03% and 114.26N, but beef grade 1 was 3.21% and 149.67N. Meanwhile, after low-temperature treatment of grade 1 beef at -26℃ for 12 hours and low-temperature aging at 0 ℃ for 14 days, the shear force was greatly reduced to 87.85N, improving overall preference, softness, dripping gravy, flavor, and chewing texture. The essential free amino acid content was as low as 22.17mg/100g in grade 1++ beef, but the contents were high at 41.31~45.11mg/100g in three samples of grade 1, and there was no change in content according to cold treatment conditions. As a specific component of beef, Taurine was 30.94~34.41mg/100g, and the difference in content was small according to beef grade, but Anserine and Creatine were low at 19.68mg/100g and 70.01mg/100g in beef grade 1++ and high at 26.38~31.23mg/100g and 154.09~167.26mg/10g in beef grade 1. The content ratio of oleic acid (c18:1)/stearic acid (c18:0) as an monounsaturated fatty acid/saturated fatty acid ratio was low at 5.29 for grade 1++ beef, but high at 6.13~6.78 for grade 1 beef. In addition, there was no trend in the content ratio of these fatty acids according to the low-temperature treatment conditions and aging period in beef grade 1. As a result of this study, it was possible to improve the quality of beef grade 1 by low-temperature treatment at -26 ℃ for 12 hours and then aging at 0 ℃ for 14 days.