• Proceedings of the Korean Institute of Surface Engineering Conference
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• 1998.11a
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• pp.15-16
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• 1998

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.4
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• pp.596-602
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• 2008

Ovotransferrin (OTf) was phosphorylated by dry-heating in the presence of pyrophosphate at pH 4.0 and $85^{\circ}C$ for 1 and 5 d, and the functional properties of phosphorylated OTf (PP-OTf) were investigated. The phosphorus content of OTf increased to 0.91% as a result of phosphorylation and the electrophoretic mobility of PP-OTf also increased. Although the solubility of dry-heated OTf slightly decreased, the decrease was reduced by phosphorylation. The stability against heat-induced insolubilization of OTf was somewhat improved by phosphorylation, but more than 70% of PP-OTf was insolubilized when it was heated at $70^{\circ}C$ for 10 min at pH 7.0. However, heat-induced insolubilization of PP-OTf was reduced when it was heated in the presence of phosphorylated ovalbumin. This may explain the excellent stability of phosphorylated egg white protein against heat-induced insolubilization which was reported previously. The emulsifying property of OTf was also somewhat improved by phosphorylation. The calcium phosphate-solubilizing ability of PP-OTf was enhanced. Although the degree of phosphorylation of OTf by dry-heating in the presence of pyrophosphate was similar to that of ovalbumin, the improvement of properties of PP-OTf was considerably different from those of phosphorylated ovalbumin.

• The Korean Journal of Nuclear Medicine
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• v.23 no.1
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• pp.13-18
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• 1989

To evaluate diagnostic accuracy of $^{99m}Tc-pyrophosphate$ (PYP) myocardial scan, we analysed 160 $^{99m}Tc-PYP$ scans (acute transmural myocardial infarction 87 cases, acute subendocardial infarction; 20 cases, unstable angina pectoris; 7 cases, other disease; 46 cases). These scans were requested by the physician in Seoul National University Hospital from Sep. 1982 to Oct. 1987. And the diagnosis was confirmed by clinical course and laboratory examinations. 1) The diagnostic sensitivity of $^{99m}Tc-PYP$ scan in acute transmural myocardial infarction was 91.2% (62/68) if scintigraphy was performed within 7 days after infarction, 57.1% (8/14) between 8th and 14th day, 20% (1/5) and after 15 days. 2) The diagnostic sensitivity of $^{99m}Tc-PYP$ scan in acute subendocardial infarction was 75% (12/16) if scintigraphy was performed within 7 days after infarction and 0% after 8 days. 3) The diagnostic specificity of $^{99m}Tc-PYP$ scan in acute myocardial infarction was 94.3% (5/53). Among 5 cases of false positive scans, 1 case was unstable angina pectoris, 2 cases were old myocardial infarction with left ventricular aneurysm, 1 case was old myocardial infarction and the remaining 1 case was cardiomyopathy.

• Molecules and Cells
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• v.27 no.6
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• pp.641-649
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• 2009

Pyrophosphate:fructose-6-phosphate 1-phosphotransferase (PFP) catalyzes the reversible interconversion of fructose-6-phosphate and fructose-1,6-bisphosphate, a key step in the regulation of the metabolic flux toward glycolysis or gluconeogenesis. To examine the role of PFP in plant growth, we have generated transgenic Arabidopsis plants that either overexpress or repress Arabidopsis PFP subunit genes. The overexpressing lines displayed increased PFP activity and slightly faster growth relative to wild type plants, although their photosynthetic activities and the levels of metabolites appeared not to have significantly changed. In contrast, the RNAi lines showed significantly retarded growth in parallel with the reduced PFP activity. Analysis of photosynthetic activity revealed that the growth retardation phenotype of the RNAi lines was accompanied by the reduced rates of $CO_2$ assimilation. Microarray analysis of our transgenic plants further revealed that the altered expression of $AtPFP{\beta}$ affects the expression of several genes involved in diverse physiological processes. Our current data thus suggest that PFP is important in carbohydrate metabolism and other cellular processes.

• Nuclear Engineering and Technology
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• v.12 no.1
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• pp.19-28
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• 1980

An instant labelling technique for lyophilized pyrophosphate with $^{99m}$Tc is described labelling yield of about 90% is obtained at the pH range 3.5-5.5 on reconstitution with sodium pertechnetate-$^{99m}$ Tc solution. The final product is controlled by a modified two dimensional paper chromatography using 85% methanol and 0.85% saline, and biodynamic investigations are performed on white mice. Generally, the less amount of stannous chloride is used. the higher labelling yield is obtained. The molar ratio of pyrophosphate to stannous chloride of 10 : 1 ~ 50 : 1 is sufficient. The more amount of reduced unbound $^{99m}$Tc is injected, the more radioactivity is incorporated in the liver. Thus. the cause of the false bone-imaging is attributable to the presence of reduced unbound $^{99m}$Tc which is known to be well adsorbed to oxidized tin colloidals. The maximum uptake ratio of bone: liver in mice, in weight basis, 35 : 1 is achieved in lime of 60 min. or so. Tile preparation is suitable for clinical investigations on patients with bone diseases.iseases.

• Clinics in Shoulder and Elbow
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• v.12 no.1
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• pp.84-88
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• 2009

Purpose: Calcium pyrophosphate dihydrate crystal deposition disease(CPPD) is a disease of the elderly and extremely rare in young individuals. If young people develop CPPD crystal deposition disease, it may be associated with metabolic diseases, such as hemochromatosis, hyperparathyroidism, hypophosphatasia, hypomagnesemia, Wilson's disease, hypothyroidism, and gout. Materials and Methods: Therefore, in young-onset CPPD crystal deposition disease, an investigation of any predisposing metabolic conditions is warranted. Conclusion: We report a case of a young female patient who presented with idiopathic CPPD crystal deposition disease at 25 years of age.

• Korean Chemical Engineering Research
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• v.52 no.5
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• pp.638-646
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• 2014

The effect of in-organic chelating agents with Fe(II) and Fe(III) in modified Fenton was evaluated to degradation BTEX (benzene, toluene, ethylbenzene, xylene). Citric acid and pyrophosphate were used in experimentals and an optimum chelating agent for BTEX degradation was determined. In $H_2O_2$/Fe(III)/citric acid, degradation of BTEX was decreased when concentration of citric acid was increased. In $H_2O_2$/Fe(III)/pyrophosphate, degradation of BTEX was increased when concentration of pyrophosphate was increased and degradation for BTEX was relatively high compared with $H_2O_2$/Fe(III)/citric acid. In $H_2O_2$/Fe(II)/chelating agents, degradation for BTEX was high and pH variation was minimized when molar ratio of Fe(II) and citric acid was 1:1. Optimum molar concentration of Fe(II), citric acid and $H_2O_2$ were 7 mM, 7mM and 500 mM for degradation of 100 mg/L of benzene to obtain best efficiency of $H_2O_2$, least precipitation of iron and best degradation.

• Development and Reproduction
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• v.11 no.2
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• pp.59-66
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• 2007

Understanding molecular mechanisms that control embryonic stem cell (ESC) self-renewal and differentiation is important for the development of ESC-based therapies. Statins, inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase), potently reduce cholesterol level. As well as inhibiting cholesterol synthesis, statins inhibit other intermediates in the mevalonate pathway such as farnesyl pyrophosphate (FPP) and geranylgeranyl pyrophosphate (GGPP), major substrates for protein isoprenylation. Studies showed that pleiotropic effects of statins beyond cholesterol lowering property arise from inhibition of protein isoprenylation that is involved in various cellular functions including proliferation and differentiation. It has been determined that statins have inhibitory effect on ESC self-renewal and stimulatory effect on ESC differentiation into adipogenic/osteogenic lineages. Importantly, statins mediate downregulation of ESC self-renewal by inhibiting RhoA-dependent signaling, independently of their choresterol-lowering properties. Understanding statin's actions on ESCs may provide important insights into the molecular mechanisms that regulate self-renewal or differentiation of ESCs.

• Bulletin of the Korean Chemical Society
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• v.31 no.3
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• pp.716-719
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• 2010

• Korean Journal of Pharmacognosy
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• v.1 no.2
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• pp.1-10
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• 1970

The cyclization of geranylgeranyl pyrophosphate followed by ionization, proton elimination or cation center migration produces dicyclic, tricyclic and tetracyclic diterpenoids. The role of some of diterpenoids in animals is well known but their precise functions in plants remain to be explained.