• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2007년도 춘계학술대회
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• pp.69-72
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• 2007

The objective of this work was to study the properties of purification of two liquid phase for exclusion of impurities in each phase. The experiments for process variables were carried out in the temperature range($H_{2}SO_{4}$ phase: $413{\sim}513$ K, $HI_{x}$ phase: $353{\sim}453$ K) and in the $N_{2}$ flow rate range($H_{2}SO_{4}$, $HI_{x}$ phase: $50{\sim}200$ mL/min). As the results, it is appeared that the principles of $H_{2}SO_{4}$ phase purification was due to stripping, evaporation and reverse bunsen reaction and $HI_{x}$ phase purification was due to stripping and reverse bunsen reaction. In purification of $H_{2}SO_{4}$ phase, the concentration rate of $H_{2}SO_{4}$ phase was controled by temperature but the temperature had few effects on yield of $H_{2}SO_{4}$. In purification of $HI_{x}$ phase, we observed products of side reactions($H_{2}S$, S) over 433 K. The purity of $HI_{x}$ phase was increased with increasing $N_{2}$ flow rate because impurites were decreased with increasing conversion of reverse reaction.

• 약학회지
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• 제47권6호
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• pp.410-416
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• 2003

Autotaxin(ATX) was originally purified from conditioned media of A2058 human melanoma cells and shown to be a potent cell motility-stimulating factor, possessing a type II nucleotide pyrophosphatase/phosphodiesterase (NPP2) activity. Recombinant ATX has recently demonstrated that human plasma lysophosholipase D is identical to ATX and uses lysophosphatidylcholine as a substrate to mediate various biological functions including tumor cell growth and motility through G-protein coupled receptor. However, despite pivotal roles of ATX on physiological or pathophysiological states, the production of ATX is solely depends on complicated purification method which employs multiple column steps, but resulted in very poor yield. This limited the use of ATX for extensive analysis. We, therefore, expressed six histidine-tagged recombinant human ATX(His-ATX) in High Five TM insect cells to improve the generation of ATX and to make simple the purification of ATX. The signal sequence of the human ATX gene was truncated and replaced with sequence of insect cell secretion signal within expression vector. In addition, codons for six histidines were added to the C-termini of 120kDa ATX cDNA construct. A simple purification scheme utilizing two-step affinity column chromatography was designed to purify His-ATX to homogeneity from the culture supernatant of transfected insect cells. Homogenous His-ATX was detected and isolated from the concentrated insect cell medium using concanavalin A agarose and nickel affinity chromatography. Purified His-ATX was in full length with ATX capacity. A combination of this expression system and purification scheme would be useful for production and purification of high-quality functional ATX for research and practical application of multiple functional motogen, ATX/NPP-2.

• 한국농공학회논문집
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• 제53권2호
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• pp.75-82
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• 2011

This study was performed to evaluate the water purification properties of bio-composites planting blocks using oyster shell and effective microorganism that have high absorption ability of heavy metals and organics to develop environmentally friendly river embankment technique contained various factors such as oyster shells, effective microorganism, porous concrete and planting embankment block. To maximize greening effect, the seeds were arbitrarily sown. In addition, in order to analyze the effect of water quality purification after the planting, the samples were collected from each designated zone 1, 7 and 30 days after steeping in water. Then, the samples were analyzed in terms of seven test items such as SS, BOD, COD, T-N, T-P, pH, etc. on the basis of the test method for water pollution. The following conclusions were reached from the test result. As a result of analysis for water quality purification for the concrete block containing the effective microorganism, it was found that the values for SS, BOD, T-N and T-P for the sample taken after 30 days were lower than the initial values, which indicated that the water purification effect had been created. The result of the water quality purification analysis for the concrete block containing oyster shell showed that the values for SS, BOD, COD and T-P for the sample taken after 30 days were lower than the initial values which also indicated that it had been effective in water quality purification.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2002년도 학술발표회 논문집(I)
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• pp.449-454
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• 2002

• 한국가스학회:학술대회논문집
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• 한국가스학회 1999년도 추계학술발표회 논문집
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• pp.187-192
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• 1999

• 한국재료학회지
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• 제26권6호
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• pp.306-310
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• 2016

To produce 4N grade high-purity silica powder from natural ore, the mineralogical characteristics of natural silica ore were investigated and their effects on the purification process were revealed. The Chinese silica mineral ore used was composed of iron and aluminum as main impurities and calcium, magnesium, potassium, sodium, and titanium as trace impurities; these trace impurities generally exist as either single oxides or complex oxides. It was confirmed that liberation and acidic washing of the impurities were highly dependent on the particle size of the ground silica ore and on its mineralogical characteristics such as the distribution and phases of existing impurities. It is suggested that appropriate size reduction of silica ore should be realized for optimized purification according to the origin of the natural silica ore. A single step purification process, the mechano-chemical washing (MCW) process, was proposed and verified in comparison with the conventional multi step washing process.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
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• pp.317-320
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• 1997

In the water purification plant, chemicals are injected for quick purification of raw water. It is clear that the amount of chemicals intrinsically depends on the water quality such as turbidity, temperature, pH and alkalinity etc. However, the process of chemical reaction to improve water quality by the chemicals is not yet fully clarified nor quantified. The feedback signal in the process of coagulant dosage, which should be measured (through the sensor of the plant) to compute the appropriate amount of chemicals, is also not available. Most traditional methods focus on judging the conditions of purifying reaction and determine the amounts of chemicals through manual operation of field experts or jar-test results. This paper presents the method of deriving the optimum dosing rate of coagulant, PAC(Polymerized Aluminium Chloride) for coagulant dosing process in water purification system. A neural network model is developed for coagulant dosing and purifying process. The optimum coagulant dosing rate can be derived the neural network model. Conventionally, four input variables (turbidity, temperature, pH, alkalinity of raw water) are known to be related to the process, while considering the relationships to the reaction of coagulation and flocculation. Also, the turbidity in flocculator is regarded as a new input variable. And the genetic algorithm is utilized to identify the neural network structure. The ability of the proposed scheme validated through the field test is proved to be of considerable practical value.

• 한국해양바이오학회지
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• 제12권1호
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• pp.66-74
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• 2020

Marine algae contain a variety of substances, such as mycosporine-like amino acids, which can defend against UV irradiation. Among them, Porphyra-334 derived from Porphyra yezoensis is attracting attention as a novel active ingredient for anti-aging cosmetics because of its excellent anti-oxidative, anti-inflammatory, and wound-healing properties through promoting skin cell migration. In this study, a process using direct current (DC) for increasing the yield of large-scale purification of Porphyra-334 was developed. When DC was applied to obtain Porphyra-334 efficiently, the purification time was shortened by approximately 1/4 compared with the process wherein DC was not applied; moreover, the yield of purification was improved.

• Journal of Microbiology and Biotechnology
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• 제9권4호
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• pp.386-392
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• 1999

A large-scale culture of hepatitis A virus in human diploid MRC-5 cells was conducted. In a roller bottle culture, the virus was grown to a maximum titer in 3 weeks after infection. Over 95％ of the cell-associated virus was excreted after culturing the infected cells in suspension media without fetal bovine serum for 3 days. The cultured virus was inactivated with formalin, concentrated by ultrafiltration, and partially purified by ultracentrifugation in a non-ionic gradient medium of Renocal. Two separate peak fractions showing high anti-HAY ELISA titer were pooled and about 40％ of HAV antigen was recovered by this purification procedure. Of the partially purified vaccine, the protein pattern in SDS-PAGE and immunogenicity in mice were compared with a commercial HAV vaccine. In SDS-PAGE, the purified vaccine in this study and the commercial vaccine showed almost the same protein pattern. The seroconversion rate of the purified vaccine in mice was not different from that of the commercial vaccine. Therefore, we could prepare a good grade of HAV vaccine by a simple purification procedure although the purification itself was not completed.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2002년도 봄 학술발표회 논문집
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• pp.717-722
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• 2002

The results of an experiment on the water purification of the porous concrete and its influence on the compressive strength are reported in this paper. Two different sizes of coarse aggregate of 5-10, and 10-20mm, and three absolute volume ratios of paste to aggregate of 30, 40 and 50 percent for a given size of aggregate were used. For the compressive strength, the concrete with the aggregate size of 5-10mm showed much higher strength than that with the aggregate size of 10-20mm. But, the compressive strength is higher when the ratio of paste to aggregate is smaller. In the water purification experiment, the amount of attached an organism on the porous concrete surface indirectly is examined by measuring the consumption of the Dissolved Oxygen (DO). The ability of the water purification is evaluated by the removal amount of the Total Phosphorus(T-P) and Total Nitrogen(T-N). The ability of the removal of the T-N and T-P in the test water is superior to a porous concrete with a smaller size of aggregate and a higher void content. These results are owing to a large specific surface area of the specimen. As a result, porous concrete using by-products has sufficient performance of water purification.