• Title/Summary/Keyword: pure lines

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Selection of Ginseng Superior Lines Tolerant to Salt Stress Through Zygotic Embryo Culture (배배양에 의한 인삼우수계통으로부터 염류 Stress 내성 계통의 선발)

  • 양덕춘;윤영상;김무성
    • Korean Journal of Plant Resources
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    • v.17 no.3
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    • pp.257-264
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    • 2004
  • Selection of stress-tolerant ginseng lines in fields is very difficult because it is almost impossible to control properly the environmental conditions of soil. On the contrary, it can be studied with ease to search for stress-tolerant ginseng lines through in vitro culture because of easy manipulation of stress conditions. This study was conducted for the selection of ginseng pure lines tolerant to salt stress. Murashige & Skoog(MS) media with 2.5 folds of KNO$_3$, NH$_4$NO$_3$, MgSO$_4$.7$H_2O$, KH$_2$PO$_4$, and CaC1$_2$.2$H_2O$ was established for the selection of ginseng pure lines tolerant to salt stress in vitro. Among 88 ginseng pure lines bred by Korea Ginseng and Tobacco Research Institute, Punggi Hwangsuk, 78093, 82886, 78135, 86024 and KG104 lines was tolerant to salt stress. For the stable production of quality Korean ginseng, genetic tolerance to salt stress is one of important factors since relatively high salt concentrations in the ginseng nursery soil environment of Korea. Ginseng inbred pure lines were tested for their tolerance to salt stress through in vitro culture technique.

Genetic characterization and population structure of six brown layer pure lines using microsatellite markers

  • Karsli, Taki;Balcioglu, Murat Soner
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.1
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    • pp.49-57
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    • 2019
  • Objective: The first stage in both breeding and programs for the conservation of genetic resources are the identification of genetic diversity in the relevant population. The aim of the present study is to identify genetic diversity of six brown layer pure chicken lines (Rhode Island Red [RIRI, RIRII], Barred Rock [BARI, BARII], Columbian Rock [COL], and line 54 [L-54]) with microsatellite markers. Furthermore, the study aims to employ its findings to discuss the possibilities for the conservation and sustainable use of these lines that have been bred as closed populations for a long time. Methods: In the present study, a total number of 180 samples belonging to RIRI (n = 30), RIRII (n = 30), BARI (n = 30), BARII (n = 30), L-54 (n = 30), and COL (n = 30) lines were genotyped using 22 microsatellite loci. Microsatellite markers are extremely useful tools in the identification of genetic diversity since they are distributed throughout the eukaryotic genome in multitudes, demonstrate co-dominant inheritance and they feature a high rate of polymorphism and repeatability. Results: In this study, we found all loci to be polymorphic and identified the average number of alleles per locus to be in the range between 4.41 (BARI) and 5.45 (RIRI); the observed heterozygosity to be in the range between 0.31 (RIRII) and 0.50 (BARII); and $F_{IS}$ (inbreeding coefficient) values in the range between 0.16 (L-54) and 0.46 (RIRII). The $F_{IS}$ values obtained in this context points out to a deviation from Hardy-Weinberg equilibrium due to heterozygote deficiency in six different populations. The Neighbour-Joining tree, Factorial Correspondence Analysis and STRUCTURE clustering analyzes showed that six brown layer lines were separated according to their genetic origins. Conclusion: The results obtained from the study indicate a medium level of genetic diversity, high level inbreeding in chicken lines and high level genetic differentiation between chicken lines.

Effect of Hybridization on Carcass Traits and Meat Quality of Erlang Mountainous Chickens

  • Yin, H.D.;Gilbert, E.R.;Chen, S.Y.;Wang, Y.;Zhang, Z.C.;Zhao, X.L.;Zhang, Yao;Zhu, Q.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.10
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    • pp.1504-1510
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    • 2013
  • Native chickens hold a significant share of the market in China. In response to the huge demand from the market, the productivity of Chinese native chickens needs to be improved. Cross breeding is an effective method to increase productivity, although it might affect meat quality. In this study, two pure lines (SD02 and SD03) of Erlang mountainous chickens were hybridized with a yellow feather and faster growing line (SD01). The effect of hybridization on carcass and meat quality (physiochemical and textural traits) was measured in the $F_1$ population at d 91 of age. The hybrids exhibited higher body weight and dressed weight, and amount of semi-eviscerated, eviscerated, breast muscle and abdominal fat (p<0.05). Abdominal fat yield also increased (p<0.05) compared to the offspring of the two pure-lines. Meanwhile, there was no significant difference in meat quality traits except for the myofiber diameter and density and the shear force of the breast muscle. Overall, the offspring of cross-lines were similar to pure lines in meat color, pH value, inosinic acid, crude protein, crude fat, dry matter, moisture content and amino acid composition in the breast muscle. These results suggest that productivity can be improved via cross-breeding while maintaining meat quality of the Erlang mountainous chicken.

Isolation and Characterization of Pure lines of Pigmentation and Morphological Mutants in Porphyra tenera Kjellman (Bangiales, Rhodophyta) (참김 (Porphyra tenera Kjellman) 색소 및 형태변이체의 순계주 분리 및 특성)

  • Hwang, Mi-Sook;Kim, Seung-Oh;Lee, Young-Soon;Park, Eun-Jeong;Kim, Seong-Cheol;Ha, Dong-Soo;Gong, Yong-Gun;Baek, Jae-Min;Choi, Han-Gu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.5
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    • pp.495-502
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    • 2010
  • Pure lines were isolated from young gametophytic blades of pigmentation and morphological mutants in Porphyra tenera. Growth, blade-shape and photosynthetic pigment content of pure lines were compared with the wild type. Growth of blade length in the wild type (W, R-B), with round shape and brown color, was fastest at $5{\sim}10^{\circ}C$ and became slower as temperature increased. The blade-shape of the wild type changed from linear to round as temperature increased. The green type (R-G), with round shape and green color, showed slower growth, and the red type (R-R) 'with round shape and red color' showed faster growth than the wild type. The blade-shapes of the green and red types changed from elliptical or linear to round as temperature increased. The phycoerythrin (PE) / phycocyanin (PC) ratio of the green type was markedly lower and the PE/PC ratio of the red type was markedly higher than that of the wild type. The linear type (L-B), with liner shape and brown color, showed faster growth in blade length than the wild type at $10{\sim}20^{\circ}C$ and maintained its linear shape at $5{\sim}15^{\circ}C$. The content of photosynthetic pigments of the linear type was similar to that of the wild type. Each of the pure lines of pigmentation and morphological mutants that were isolated in the present study showed particular patterns in growth, blade-shape and photosynthetic pigment composition. Therefore, they are expected to be useful as new varieties by themselves and to be available for breeding and biotechnological studies.

A Study on the natural Convection and Radiation in a Rectangular Enclosure with Ceiling Vent (천장개구부를 갖는 정사각형 밀폐공간내의 자연대류-복사 열전달에 관한 연구)

  • Park Chan-kuk;Chu Byeong-gil;Kim chol;Jung Jai-hwan
    • Journal of the Korean Institute of Gas
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    • v.2 no.1
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    • pp.28-39
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    • 1998
  • This study investigated the natural convection and radiation in a rectangular enclosure with ceiling vent experimentally and numerically. A heat source is located on the center of the bottom surface. The analysis was peformed a pure convection and is combination of natural convection and radiation. The shape of the considered two dimensional model is a square whose center of ceiling($30\%$) is opened. The numerical simulations are carried out for the pure natural convection case and the combined heat transfer case by using the SIMPLE algorithm. For the turbulent flow, Reynolds stresses are closed by the standard $k-{\epsilon}$ model and the wall function is used to determine the wall boundary conditions. The experiment was performed on the same geometrical shape as the computations. The radiative heat transfer is analized by the S-N discrete ordinates method. The results of pure natural convection are compared with those of combined heat transfer by the velocity vectors, stream lines, isothermal lines. The results obtained are as follows 1. Comparing the results of pure convection with those of the combined convection-radiation through the shape of stream lines, isothermal lines are similar to each other. 2. The temperature fields obtained by numerical method are compared to those obtained by experimental one, and it is found that they are showed mean relative error $8.5\%$. 3. Visualization bt smoke is similar to computational results.

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Spitzer and Herschel observations of protostellar outflows in L1251B

  • Choi, Yunhee;Lee, Jeong-Eun;Green, Joel D.;Maret, Sebastien;Yang, Yau-Lun;Bergin, Edwin A.;Blake, Geoffrey A.;Boogert, Abraham. C.A.;Di Francesco, James;Evans, Neal J. II;Pontoppidan, Klaus M.;Sargent, Annelia I.
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.2
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    • pp.81.1-81.1
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    • 2017
  • L1251B is an excellent example of a small group of pre- and protostellar objects in low-mass star-forming region. Previous interferometer data with a high angular resolution resolved the outflows associated with L1251B into a few components. To understand the physical conditions of the protostellar outflows in L1251B, we mapped this region spectroscopically with Spitzer/IRS and obtained spectral line data from Herschel/PACS. Spitzer/IRS provides the S(0)-S(7) pure rotational lines of H2 as well as fine-structure emissions produced in shocks such as S, [Si II], and [Fe II] and it is a powerful tool for studying shocked interstellar gas. In addition, [O I] lines observed with Herschel/PACS are described well by J-type shock models expected in the outflows from protostars. We will present an analysis of the L1251B protostellar outflow in the H2 pure rotational lines and fine-structure emissions.

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Breeding of Korean Ginseng (Panax ginseng C. A. Meyer)

  • Kwang-Tae Choi;Woo
    • Proceedings of the Ginseng society Conference
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    • 1998.06a
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    • pp.96-102
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    • 1998
  • A lot of individual ginseng plants were selected in the farmer's fields to develop new ginseng varieties with desirable traits. Among them, a promising line has been developed through comparative cultivation of several lines selected with pure line separation of local raced. It was then designated as "KC (denotes Korean Ginseng)" and tested in the regional yield and adaptation trials for 10 years. KG lines grew vigorously after 4 years of age. Especially, KG 102 line arson운 them showed traits of multiple and short stems. As for the root characters, the length of taproot of KG 101 line was longer than that of local race, Jakyung-jong, or other lines ailed the root weight of KG 102 line waIn 15 % higher than that of local race. In general, KG 101, KG 103, KG 104, and Hwangsauk-jong had good root shape. Total amount of ginsenosides of ginseng taproot was the highest in KG 103 line than in local race and other lines. In these studies, we, elected three superior lines, KG 101, KG 102, and KG 103, having characteristic of good root shape, high yield, and large amount of ginsenoside, respectively.

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Improvement of Cooling Technology through Atmosphere Gas Management

  • Renard, Michel;Dosogne, Edgar;Crutzen, Jean-Pierre;Raick, Jean-Marc;Ma, Jia Ji;Lv, Jun;Ma, Bing Zhi
    • Corrosion Science and Technology
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    • v.8 no.6
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    • pp.217-222
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    • 2009
  • The production of advanced high strength steels requires the improvement of cooling technology. The use of high cooling rates allows relatively low levels of expensive alloying additions to ensure sufficient hardenability. In classical annealing and hot-dip galvanizing lines a mixing station is used to provide atmosphere gas containing 3-5% hydrogen and 97-95% nitrogen in the various sections of the furnace, including the rapid cooling section. Heat exchange enhancement in this cooling section can be insured by the increased hydrogen concentration. Drever International developed a patented improvement of cooling technology based on the following features: pure hydrogen gas is injected only in the rapid cooling section whereas the different sections of the furnace are supplied with pure nitrogen gas; the control of flows through atmosphere gas management allows to get high hydrogen concentration in cooling section and low hydrogen content in the other furnace zones. This cooling technology development insures higher cooling rates without additional expensive hydrogen gas consumption and without the use of complex sealing equipments between zones. In addition reduction in electrical energy consumption is obtained. This atmosphere control development can be combined with geometrical design improvements in order to get optimised cooling technology providing high cooling rates as well as reduced strip vibration amplitudes. Extensive validation of theoretical research has been conducted on industrial lines. New lines as well as existing lines, with limited modifications, can be equipped with this new development. Up to now this technology has successfully been implemented on 6 existing and 7 new lines in Europe and Asia.

Design of Structured Surfaces for Directional Mobility of Droplets

  • Osada, Takehito;Kaneko, Arata;Moronuki, Nobuyuki;Kawaguchi, Tomoyo
    • International Journal of Precision Engineering and Manufacturing
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    • v.9 no.3
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    • pp.13-17
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    • 2008
  • This paper deals with the directional mobility of droplets on structured surfaces. Structured surfaces were micro-patterned with rectangular lines and spaces of varying pitch and height in the sub-millimeter range. The material used was polydimethylsiloxane, which is hydrophobic and wettable by oil. First, we studied the effect of the structural design on the sliding angle of pure water or oil through experiments. For pure water droplets, we found that a wider pitch enhanced the directionality. On the other hand, oil droplets spread along the groove because of their low surface tension and strong capillary force. The directionality of the sliding angle of oil droplets was larger than that of pure water, especially when the groove was narrower and deeper. Second, we poured a large amount of liquid on the structure and evaluated the removal rate on the tilted surface. We found that a parallel structure enhanced the liquid mobility for both pure water and oil.

A JONCKHEERE TYPE TEST FOR THE PARALLELISM OF REGRESSION LINES

  • Jee, Eunsook
    • The Pure and Applied Mathematics
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    • v.20 no.2
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    • pp.109-116
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    • 2013
  • In this paper, we propose a Jonckheere type test statistic for testing the parallelism of k regression lines against ordered alternatives. The order restriction problems could arise in various settings such as location, scale, and regression problems. But most of theory about the statistical inferences under order restrictions has been developed to deal with location parameters. The proposed test is an application of Jonckheere's procedure to regression problem. Asymptotic normality and asymptotic distribution-free properties of the test statistic are obtained under some regularity conditions.