• Title/Summary/Keyword: pulsed electric field

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Physiological Changes of Saccharomyces cerevisiae by High Voltage Pulsed Electric Field Treatments (고전압 펄스 전기장 처리에 의한 Saccharomyces cerevisiae의 생리적 변화)

  • Park, Hee Ran;Yoon, So Jung;Park, Han-Sul;Shin, Jung-Kue
    • Korean Journal of Food Science and Technology
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    • v.45 no.5
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    • pp.590-597
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    • 2013
  • High voltage pulsed electric fields (PEF) treatment is a promising non-thermal processing technology that can replace or partially substitute for thermal processes. The aim of this research was to investigate the microbial inactivation mechanisms by PEF treatment in terms of physiological changes to Saccharomyces cerevisiae. PEF was applied at the electric field strength of 50 kV/cm, treatment time of 56 ${\mu}s$ and temperature of $40^{\circ}C$. The microbial cells treated with PEF showed loss of salt tolerance on the cell membrane and collapse of the relative pH gradient on in-out of cells. Cell death or injury resulted from the breakdown of homeostasis, decreased $H^+$-ATPase activity, and loss of glycolysis activity.

Effects of Pulsed Electric Fields on Juice Expression Characteristics of Malus pumila Fruit (고전압 펄스 전기장 처리가 사과의 착즙 특성에 미치는 영향)

  • Lim, Jeong-Ho;Ahn, Seong-Hwan;Lee, Dong-Un;Kim, Young-Ho;Park, Kee-Jai
    • Food Science and Preservation
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    • v.19 no.5
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    • pp.665-671
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    • 2012
  • The effects of pretreatment by pulsed electric fields (PEFs) on the juice expression characteristics of the Malus pumila fruit were investigated. Fresh fruits were divided into quarters, were produced on a laboratory scale (100 g apples per lot) by pretreatment with electric fields at two different field intensities (1, 2 kV/cm; n=50, 100, 200, and 400 pulses), and were then pressed at room temperature. Relative to the control samples, the juice yield increased with increasing field intensities. The total phenolics and antioxidant activity were higher in the juice from the PEF-treated fruit than in the juice from the untreated fruit. There was no significant difference in soluble-solid and reducing sugar contents between the PEF-treated and untreated fruits. These results suggest that PEF pretreatment may be useful for increasing the juice yield, total phenolics, and antioxidant activity of the Malus pumila fruit.

A removal characteristics of NOx at the cylinderical plasma reactor with magnetic field (자계가 인가된 원통형 플라즈마 반응기에서 질소산화물(NOx)의 제거특성)

  • Lee, Dong-Hoon;Lee, Tae-Geun;Oh, Jung-Min;Lee, Doo-Hee
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2004.05b
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    • pp.104-108
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    • 2004
  • The effect of magnetic field was measured on NOx removal for cylinder-wire plasma reactor with magnetic field applied to electric field vertically. Power was supplied to plasma reactor using rotating spark gap switch. Consumption power increased with increasing discharge voltage. When magnetic field was applied to electric field vertically, consumption power was less than that without magnetic field because of lorenz's force. NOx removal rate of plasma reactor with magnetic field were higher, 10-15%, than that of plsama reactor without magnetic field. And NOx removal rate decreased with increasing gas flow rate.

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Changes of Chemical Concentrations during Pulsed Plasma Process of Silane (실란 펄스 플라즈마 공정에서의 화학농도 변화)

  • Kim, Dong-Joo;Kim, Kyo-Seon
    • Journal of Industrial Technology
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    • v.25 no.A
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    • pp.141-149
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    • 2005
  • We investigated numerically the evolutions of several chemical species which are important for film growth and particle generation in the pulsed $SiH_4$ plasmas. During the plasma-on, the $SiH_x$ concentration increases with time mainly by the generation reaction from $SiH_4$, but, during the plasma-off, decreases because of the hydrogen adsorption reaction. During the plasma-on, the concentrations of negative ions increase with time by the polymerization reactions of negative ions and those become almost zero in the sheath regions because of the electrostatic repulsion. During the plasma-off, the concentrations of negative ions decrease with time by the neutralization reactions with positive ions and some negative ions can diffuse toward the sheath regions because there is no electric field inside the reactor. The polymerized negative ions of higher mass can be reduced successfully by using the pulsed plasma process.

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Effect of Magnetic Field on NOx Removal for Wire-Plate Plasma Reactor (선대 평판형 플라즈마 반응기에서 NOx 제거에 미치는 자계의 영향)

  • Park, J.Y.;Son, S.D.;Han, S.B.;Lee, D.H.;Kim, J.D.;Mun, Y.H.
    • Proceedings of the KIEE Conference
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    • 1999.07e
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    • pp.2251-2252
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    • 1999
  • In this paper, the effect of magnetic field was measured on NOx removal characteristics for wire-plate plasma reactor with magnetic field applied to electric field vertically. NOx from simulated diesel engine flue gas are decomposed by the corona discharge of DC, AC and Pulsed voltages in wire-plate reactor. Consumption power increased with increasing discharge voltage. When magnetic field was applied to electric field vertically, consumption power decreased. NOx removal rate and arc transition voltage of plasma reactor with magnetic field were higher than those of plasma reactor without magnetic field.

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Physiological Properties of Microbial Cells Treated by Pulsed Electric Field(PEF) (고전압 펄스 전기장 처리된 미생물 세포의 생리특성)

  • Kim, Kyung-Tack;Kim, Sung-Soo;Choi, Hee-Don;Hong, Hee-Doo;Ha, Sang-Do;Lee, Young-Chun
    • Korean Journal of Food Science and Technology
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    • v.31 no.2
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    • pp.368-374
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    • 1999
  • This study was designed to investigate effects of pulsed electric field (PEF) treatment on physiological changes of microbial cells, using domestically fabricated pilot scale PEF device. The effect of non-thermal PEF treatment on physiological characteristics of microorganisms was determined by salt resistance, the amount of UV absorbents, cell staining, recovery rate of defected cells, and changes in structure of cell membrane. Salt resistance of Escherichia coli, Bacillus subtilis and Rhodotorula minuta was examined after PEF treatment at 40 kV/cm, 84 pulse, $10{\mu}s$ pulse duration. Approximately $1\;log_{10}$ cell number of viable microorganisms was decreased by addition of salt. PEF treatment significantly increased the amount of UV absorbents at 260 and 280 nm because of leakage from damaged cell membrane by PEF treatment. Although three kinds of microorganisms treated by PEF were difficult to be observed due to their cell membrane damage, untreated cells were clearly observed by a microscope. PEF-treated R. minuta was not stained by methylene blue due to cell membrane defect. When E. coli, B. subtilis and R. minuta were cultured after PEF treatment, they showed 5, 4, and 8 hr longer lag phase, respectively, compared to control, but growth rates were not affected.

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Effect of Magnetic Field on NOX Removal for Wire-Plate Plasma Reactor (선대 평판형 플라즈마 반응기에서 NOX 제거에 미치는 자계의 영향)

  • Park, Jae-Yun;Go, Hui-Seok;Son, Seong-Do;Lee, Dong-Hun;Kim, Jong-Dal
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.49 no.2
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    • pp.120-124
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    • 2000
  • In this paper, the effect of magnetic field was measured on NOx(NO+NO2) removal and consumption power for wire-plate plasma reactor with magnetic field applied to electric field vertically. NOx of the simulated diesel engine flue gas were removed by the corona discharge generated by DC, AC and Pulsed voltages in wire-plate reactor. Consumption power increased with discharge voltage. However, when magnetic field was applied to electric field vertically, consumption power slightly decreased. NOx removal rate and arc transition voltage for plasma reactor with magnetic field were higher than those for plasma reactor without magnetic field. Consumption power decreased, however NOx removal significantly increased, when water vapour bubbled by dry air was put into simulated flue gas.

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Sterilization Experiment by High-Voltage Pulsed Electric Fields (고전압 전기장에 의한 살균실험)

  • Kang, Sung-Mi;Park, Su-Ji;Kim, Woo-Jin;Chang, In-Sung;Lee, June-Ho
    • Proceedings of the KIEE Conference
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    • 2005.07c
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    • pp.2211-2213
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    • 2005
  • Recently, the membrane technology has actively been applied to make generating water because the convention; chemical treatment technology for purifying water has caused second environmental pollution. In spite of its ecological advantages, this membrane technology has some drawbacks such as ease of membrane contamination, efficiency and running cost. The purpose of this research is reduction of membrane contamination by applying high voltage pulsed electric field to the water prior to its penetrating membrane.

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Discharge characteristics of FFL as applied voltage variation (인가 전압의 변화에 따른 FFL(Flat Fluorescent Lamp)의 방전특성)

  • 윤성현;박철현;조민정;임민수;권순석;임기조
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2000.11a
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    • pp.379-382
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    • 2000
  • The characteristics of Xe discharge lamp(Mercuryless lamp) are described in this paper. In this paper, FFL is operated by sine wave and pulsed source. We apply V-Q Lissajous' figure for the discharge measurements of FFL which has the electrodes covered with dielectric. When FFL is operated by sine wave source, the characteristics are similar to DBD(Dielectric Barrier Discharge) and SD(Silent Discharge). And we compared the characteristics of FFL which is operated with sine wave and pulsed discharge by using V-Q Lissajous' figure method. When FFL is operated with pulsed, the discharge current flows after the applied voltage is risen. As the duty ratio increases the electric field becomes strong and much more xenon ions are produced. And the number of metastable xenon atoms seem to increase, therefore, the phosphor radiation after the cut off of voltage increases compared with the first peak of radiation. Consequently, the 172㎚ radiation becomes strong as the duty ratio increases.

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