• 한국식물학회:학술대회논문집
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• 한국식물학회 1999년도 제13회 식물생명공학심포지움 New Approaches to Understand Gene Function in Plants and Application to Plant Biotechnology
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• pp.11-15
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• 1999

In order to evlauate feasibility of the gene tagging by the maize transposable element Ac in heterologous plant systems, we have investigated physical distances and directions of transposition of the element in Arabidopsis thaliana and tobacco cultured cell line BY-2. We prepared a T-DNA construct that carried a non-autonomous derivative of Ac with a site for cleavage by endonuclease I-Scel (designated dAc-I-RS element). Another cleavage site was also introduced into the T-DNA region outside dAc-I-RS. A number of transgenic Arabidopsis plants were generated, each of which had a single copy of the T-DNA at a different chromosomal location. To examine the pattern of transposition, three out of these transgenic plants were crossed with the Arabidopsis plant that carried the gene for Ac transposase and progeny in which dAc-I-RS had been transposed were isolated. After digestion of the genomic DNA of these progeny with I-SceI, sizes of segment of DNA were determined byd pulse-field gel electrophoresis. We also performed linkage analysis for the transposed elements and sites of mutations near the elements. Our results with three transgenic lines showed that 50% of all transposition events had occurred within 1,700 kilo-base pairs (kb) on the same chromosome, with 35% within 200 kb, and that the elements transposed in both directions on the chromosome with roughly equal probability. The data thus indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis. In addition, determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites. In the present paper, we report typical examples of such gene isolation studies.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1985년도 워크샵 및 심포지엄 북한산국립공원의 식생
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• pp.51-57
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• 1985

In order to evlauate feasibility of the gene tagging by the maize transposable element Ac in heterologous plant systems, we have investigated physical distances and directions of transposition of the element in Arabidopsis thaliana and tobacco cultured cell line BY-2. We prepared a T-DNA construct that carried a non-autonomous derivative of Ac with a site for cleavage by endonuclease I-Scel (designated dAc-I-RS element). Another cleavage site was also introduced into the T-DNA region outside dAc-I-RS. A number of transgenic Arabidopsis plants were generated, each of which had a single copy of the T-DNA at a different chromosomal location. To examine the pattern of transposition, three out of these transgenic plants were crossed with the Arabidopsis plant that carried the gene for Ac transposase and progeny in which dAc-I-RS had been transposed were isolated. After digestion of the genomic DNA of these progeny with I-SceI, sizes of segment of DNA were determined byd pulse-field gel electrophoresis. We also performed linkage analysis for the transposed elements and sites of mutations near the elements. Our results with three transgenic lines showed that 50% of all transposition events had occurred within 1, 700 kilo-base pairs (kb) on the same chromosome, with 35% within 200 kb, and that the elements transposed in both directions on the chromosome with roughly equal probability. The data thus indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis. In addition, determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites. In the present paper, we report typical examples of such gene isolation studies.

• 대한임상독성학회지
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• 제7권2호
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• pp.77-82
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• 2009

Purpose: In highly doses, endosulfan lowers the seizure threshold and elicits central nervous system stimulation, which can result in seizures, respiratory failure, and death. Management of seizure control is essential for survival and prognosis of intoxicated patients. This study assessed whether seizure time was an independent predictor mortality in patients with endosulfan poisoning. Methods: This retrospective study enrolled patients with endosulfan poisoning presenting to Masan Samsung Hospital and Gyeongsang National University Hospital from January 2003 to December 2008. The data were collected from clinical records and laboratory files. Using a multivariate logistic analysis, data on the total population was retrospectively analyzed for association with mortality. Results: Of the 24 patients with endosulfan poisoning, nineteen (79.1%) experienced seizure. The patients in the seizure group showed significantly lower Glasgow coma scale score, base excess, bicarbonate, and significant existence of mechanical ventilation, as compared to the non seizure group (n=5). Seizure, Glasgow coma scale score, systolic blood pressure, bicarbonate level, need for respiratory support, pulse rate, respiratory rate, pH, base excess, and seizure time were associated with mortality. The fatality rate of endosulfan poisoning was 54.1% with higher mortality among patients experiencing. Longer seizure time was associated with higher mortality. Conclusion: Seizure time can be a significant independent predictor of mortality in patients with acute endosulfan poisoning. Physicians should aggressively treat for seizure control in patients with acute endosulfan poisoning.

• 대한의용생체공학회:의공학회지
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• 제35권4호
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• pp.75-80
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• 2014

This paper proposed a method that can automatically classify sleep apnea by using features extracted from pulse rate variability(PRV) signals induced from piezo snoring sensor for patients with obstructive sleep apnea(OSA). We have extracted eight features(NN, SDNN, RMSSD, NN10, NN50, LF, HF and LF/HF ratio) based on time and frequency analyses of PRV. Sleep apnea was classified by a linear discriminant analysis(LDA). A performance was evaluated using snore recordings from 13 patients with OSA (ages: $54.5{\pm}10.5$ years, body mass index: $26.3{\pm}2.5kg/m^2$, apnea-hypopnea index: $19.2{\pm}6.0/h$). The sensitivity and specificity were $78.9{\pm}0.9%$ and $78.9{\pm}0.9%$ for training set and $77.7{\pm}10.9%$ and $79.0{\pm}2.8%$ for test set, respectively. Our study demonstrated the feasibility of implementing a piezo snoring sensor based on a portable device as a simple and cost-effective solution for contributing to the OSA screening.

• 대한치과마취과학회지
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• 제6권2호
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• pp.89-97
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• 2006

Background: The aim of this study was to examine the difference of $SpO_2$, PR, $EtCO_2$, RR with submucosal injection of midazolam to oral chloral hydrate and hydroxyzine for pediatric patients Methods: Thirty two sedation cases were performed in this study. Patients were randomly classified into one group taking oral CH (60 mg/kg). hydroxyzine (1 mg/kg) and submucosal injection of midazolam (0.1 mg/kg) and the other group recieving oral CH (50 mg/kg), hydroxyzine (1 mg/kg) and submucosal injection of midazolam (0.2 mg/kg). For evaluating the depth of sedation. data including saturation percentage of oxygen ($SpO_2$), pulse rate (PR), end-tidal carbon dioxide ($EtCO_2$), respiratory rate (RR) and the behavior scale were checked every 2 minutes and were collected for only 40 minutes from the beginning of treatment and were analyzed using Two independent sample T-test. Results: Analysis showed no significant difference in the mean $SpO_2$, PR, $EtCO_2$, RR during sedation between two groups (P > 0.05). The values of $SpO_2$, PR, $EtCO_2$ and RR for both groups remained within the normal values. Conclusions: The results of this present study indicate that combination of oral CH, hydroxyzine, nitrous oxide gas inhalation and submucosal injection of midazolam improved the sedation quality without compromising safety.

• 한국전자파학회논문지
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• 제24권1호
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• pp.82-90
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• 2013

본 논문에서는 고주파 회로 모델링을 이용하여 전기자동차의 모터 구동 시스템으로부터 방출되는 전도성 전자파 노이즈를 시스템-레벨에서 분석하였다. 관련 전도 방출의 주요 원인은 모터 구동 시스템에서 사용하는 펄스폭 변조방식의 스위칭 동작에 기인하며, 이러한 전도 방출은 공통-임피던스 결합 및 유도성 결합을 통해 AM/FM 주파수 대역에서의 무선주파수 간섭을 유발한다. 이러한 문제를 분석하기 위해 모터 구동 시스템을 구성하고 있는 IGBT와 고압 커패시터, 인버터, 모터 및 고전압 케이블과 버스 바에 대한 기본 회로는 물론, 각 부분에서 존재하는 기생 성분 및 비선형 특성을 해석하여 모터 구동 시스템 전체를 포함한 시스템-레벨의 고주파 등가회로 모델을 제안하였다. 이러한 모델을 이용한 모터 구동시스템의 전도 방출 특성을 시뮬레이션하고, 측정하였으며, 비교적 큰 해석구조임에도 불구하고 두 결과가 비교적 잘 일치함을 확인하였다. 향후 이러한 접근방법이 전기자동차의 전자파 적합성 설계에 효과적으로 사용될 수 있을 것으로 기대한다.

• 조명전기설비학회논문지
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• 제21권6호
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• pp.55-64
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• 2007

본 논문은 부하직렬 고주파 공진 DC/DC 컨버터에 가변 가능한 공진커패시터를 병렬로 삽입한 LCC형 고주파 공진 DC/DC 컨버터의 특성과 설계 예를 나타내고 있으며, 또한 턴-오프시 스위칭 손실을 줄이기 위해 ZVS를 가지는 소프트 스위칭을 사용하였다. 제안 컨버터는 PFM(Pulse Frequency Modulation) 스위칭 패턴을 사용하여 동작되며, PFM 제어에 의해 제안 회로의 출력전압을 제어하였다. 병렬커패시터의 커패시턴스의 변화에 따라, DC/DC 컨버터의 분석은 일반적으로 무차원화 파라미터를 사용하여 나타내었고, 회로 동작 특성은 스위칭 주파수와 파라미터로 행하였다. 또한 본 논문은 특성평가에 의해 제안 DC/DC 컨버터의 동작 특성과 회로 설계 방법을 제시하였다. 더욱이 본 논문은 실험을 통해 이론 분석의 정당성을 입증하였다. 향후 제안한 DC/DC 컨버터는 반도체 및 FPD의 클린룸에 선형이동 시스템의 비접촉 전원장치에 적용 가능하다고 생각된다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2008년도 하계학술대회 논문집 Vol.9
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• pp.76-76
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• 2008

The resonance properties due to the surface plasmon(SP) excitation of metal nanoparticles make the nanocomposite films promising for various applications such as optical switching devices. In spite of the well-known ultra-sensitive operation of optical switches based on a guided wave, the application of nanocomposite film(NC) has inherent limitation originating from the excessive optical loss related with the surface plasmon resonance(SPR). In this study, we addressed this problem and present the experimental and theoretical analysis on the pump-probe optical switching in prism-coupled Au(1 vol.%):$SiO_2$ nanocomposite waveguide film. The guided mode was successfully generated using a near infrared probe beam of 1550 nm and modulated with an external pump beam of 532 nm close to the SPR wavelength. We extend our approach to ultra-fast operation using a pulsed laser with 5 ns pulse width. To improve the switching speed through the reduction in thermal loading effect accompanied by the resonant absorption of pump beam light, we adopted a metallic film as a coupling layer instead of low-index dielectric layer between the high-index SF10 prism and NC slab waveguide. We observed great enhancement in switching speed for the case of using metallic coupling layer, and founded a distinct difference in origin of optical nonlinearities induced during switching operation using cw and ns laser.

• 한국기계가공학회지
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• 제16권5호
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• pp.69-77
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• 2017

This paper introduces an optical encoder using the reflection in the slit. The digital optical encoder is a sensor to generate a pulse according to the displacement. An optical encoder is composed of 3 parts: light source, slit plate and light-receiving element. In a conventional encoder, one slit produces one signal. The resolution of the digital optical encoder is determined by the number of slits in the encoder plate. The small slit size is most important among the factors that determine the resolution in a generic-type optical encoder. However, a small slit has low productivity and technical difficulties, so analog optical encoders have emerged as an alternative. Nonetheless, this alternative requires additional circuitry and equipment because of the noise and drafts in the analog signals. A new sensor is presented in this paper with a high resolution and a slit of the same size using the reflection in the slit. Then, the path of the light that passes through the slit ccording to the shape was analyzed, and some paths were expressed in the mathematical expressions. In addition, the optical paths were analyzed in the rectangular, octagonal, and circular encoders, and shown the obtained number of signals per slit by using them. Thus, we confirm that this method has the best performance in circle-shaped slits.

• 대한전자공학회논문지TC
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• 제45권12호
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• pp.152-158
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• 2008

천해 환경에서 정합필터와 LFM(linear frequency modulation) 펄스를 사용하는 능동소나의 탐지성능은 비백색잡음인 잔향에 의해 크게 저하될 수 있다. 이 경우 잔향의 영향을 줄이기 위해 일반적으로 정합필터에 선행하여 백색화 필터를 사용한다. 기존에는 탐지 블록과 선행 블록의 잔향이 통계적으로 정상성을 유지한다고 가정하고 선행 블록의 잔향 특성을 이용하여 탐지 블록의 잔향을 추정하고 백색화 하였다. 잔향의 정상성은 인접 블록 사이뿐만 아니라 좀더 넓은 범위에서 유지될 수 있다. 이 경우 더 많은 인접 블록을 이용하면 탐지 블록의 잔향을 더 정밀하게 추정할 수 있을 것이다. 본 논문에서는 실제 해안에서 획득한 잔향 신호를 분석하여 적절한 추정 블록의 범위를 구한다. 그리고 추정 블록의 범위에 따른 선-백색화 정합필터의 성능을 비교, 분석한다.