• Journal of Pharmaceutical Investigation
• /
• 제5권4호
• /
• pp.24-31
• /
• 1975

This paper attempts to investigate the effect of proteolytic enzyme on the absorption and excretion of pyrazinamide. The rat small intestinal absorption of pyrazinamide in the presence of proteolytic enzyme such as chymotrypsin and compounding enzyme (chymotrypsin and trypsin) are increasingly absorbed, but in the trypsin are similar to that of control. Blood levels of pyrazinamide after rabbit's duodenum injection are significantly enhenced to correspond to 112-120% by proteolytic enzymes concentration respectively, but both on the high concentration of chymotrypsin and the low concentration of trypsin are insignificantly enhenced. Proteolytic enzymes do not give the effect on clearance of pyrazinamide. Proteolytic enzymes give the effect on absorption of pyrazinamide, but do not give the effect on excretion of pyrazinamide.

• 한국수산과학회지
• /
• 제32권4호
• /
• pp.458-465
• /
• 1999

Distribution of the proteolytic activities of crude pretense extracted from the viscera of ten kinds of fish was examined. Their proteolytic activities on proteinous substrates (azocasein, hemoglobin, and casein) from the viscera of anchovy, bastard flatfish, mackerel and red sea bream were higher than those of other fishes, and the crude pretenses were further fractoinated with acetone or ammonium sulfate. Optimum concentrations for pretenses fractionation were $0\~55\%$ for acetone and $30\~70\%$ for ammonium sulfate. The fractionated viscera pretense of mackerel showed the highest proteolytic activity among four kinds of fishes. Activities of cathepsin D- and pepsin-like enzymes at pH 3.0, cathepsin L-, B-, H- and G-like enzyme at pH 6,0, and Hypsin- and chymotrypsin- like enzymes (pH 8.0) were detected in the fractionated viscera pretense, whereas activities of cathepsin L- and chymoeypsin-like enzyme were observed in commercial pretenses. Proteolytic activities of Alcalase, Protamex, and Aroase AP-10 for azocasein were slightly higher than the fractionated viscera pretenses, but their amidolytic activities at pH 6.0 and 8.0 toward synthetic substrates were lower than counterpart. The fractionated pretenses from fish viscera would be utilized as commercial pretenses.

• 한국식품영양학회지
• /
• 제6권2호
• /
• pp.89-95
• /
• 1993

A Nuluk, a Korean traditional Koji for brewing, was made with wheat flour and Aspergillus oryzae L2 which had a good aroma and strong abilities In producing saccharogenic and dextrogenic enzymes. The cultural conditions for the production of saccharogenic and proteolytic enzymes were tested. The productivity of dextrogenic enzyme was improved when Nuluk was made with unsteamed wheat flour as compared with steamed one, but that of proteolytic enzyme was reduced. The addition of water containing 0.5% hydrochloric acid was unfavorable for the production of those two enzymes. The optimum ratio of water added to wheat flour for the production of those two enzymes was 28$^{\circ}C$ on the basis of wheat flour, The productivity of saccharogenic enzyme was enhanced when the Nuluk was molded after 20 hours of precultivation, but that of proteolytic enzyme was reduced as compared with no molding. The optimum temperatures for the production of saccharogenic enzyme and proteolytic enzyme were 36$^{\circ}C$ and 28$^{\circ}C$, respectively.

• 한국식품영양학회지
• /
• 제7권1호
• /
• pp.51-57
• /
• 1994

A nuluk, a Korean traditional koji for brewing, was made with wheat flour and Aspergillus usamii mot. shirousamii S1 which had strong abilities in producing amylase and protease. The cultural conditions for the production of saccharogenic and proteolytic enzymes were tested. The productivities of saccharogenic and dextrogenic enzymes were improved when nuluk was made with unsteamed wheat flour as compared with steamed one, but those of proteolytic enzyme and organic acid were reduced. The addition of water containing 0.5% of hydrochloric acid was unfavorable for the production of saccharogenic, dextrogenic and proteolytic enzymes. The optimum ratios of water added to wheat flour for the production of saccharogenic enzyme and proteolytic enzyme were 32% and 28%, respectively on the basis of wheat flour. The optimum temperatures for the production of saccharogenic enzyme and proteolytic enzyme were 36$^{\circ}C$ and 28$^{\circ}C$, respectively. The activity of saccharogenic enzyme reached its maximum after 120 hours of cultivation at 36$^{\circ}C$, but that of proteolytic enzyme 96 hours. The productivity of saccharogenic enzyme was enhanced when the nuluk was molded after 24 hours of precultivation but that of proteolytic enzyme was reduced as compared with no molding.

• 한국축산식품학회지
• /
• 제38권1호
• /
• pp.143-151
• /
• 2018

The effects of proteolytic enzymes (bromelain and bromelain+papain) and a ginger extract were assessed on collagen content and solubility, thermal shrinkage temperature of connective tissue, pH, cooking loss, drip loss, and Warner-Bratzler shear force (WBSF) of M. pectoralis profundus isolated from the beef brisket cut. Both proteolytic enzymes and ginger extract led to a significant increase in cooking loss and collagen solubility compared with untreated controls. On the other hand, the peak ($T_p$) thermal shrinkage temperature markedly decreased in all treatments compared with those in controls. Samples treated with bromelain, bromelain + papain, and ginger extract showed a significant decrease in WBSF by 36%, 40%, and 37%, respectively, compared with untreated controls. Our findings suggest that ginger extract are useful for post-mortem tenderization of meat containing high levels of collagen, compared to control even though, bromelain and bromelain + papain treatments have higher collagen solubility than ginger extract.

• Journal of Pharmaceutical Investigation
• /
• 제4권1_2호
• /
• pp.31-38
• /
• 1974

The effort of proteolytic enzyme on the absorption and excretion of sulfadimethoxine are investigated in this paper. The rat small intestinal absortion of sulfadimethoxine in the presence of proteolytic enzymes such as chymotrypsin and trypsin are increasingly absorbed more than 20% during 3 hours. Blood levels of sulfadimethoxine following oral administration are not effected by proteolytic enzyme, but both on the high concentration of chymotrypsin and the low concentration of trypsin at only 3 and 4 hours are increased(P<0.05) from that of the control . Blood levels of sulfadimethoxine after duodenum injection are remakably enhensed to correspand to 18.5-25.0% (P<0.01) by the proteolytic enzyme concentration respectively. Proteolytic enzymes did not give influence on excretions of sulfadimethoxine after duodenum and oral administration.

• 미생물학회지
• /
• 제18권1호
• /
• pp.15-19
• /
• 1980

In order to study on the pigment and protease of Serratia marcescens, the correlation between protease activity and pigment formation was investigated. The results are as follows ; (1) The protease activity exhibitied two pH optima 6.0 and 7.5, respectively. (2) The optimal temeprature of proteolytic activity was $45^{\circ}C$. With these-results, it is suggested that the proteolytic enzymes of Serratia masrecescens is stable at neutral pH range and more active at the high temeprature than lthat of otehr proteolytic enzymes.

• 한국식품과학회지
• /
• 제5권3호
• /
• pp.174-177
• /
• 1973

한우(韓牛)의 사태근육(筋肉)에 단백질분해효소(蛋白質分解酵素)를 첨가(添加)해서 total tryptophan 과 free tryptophan을 spectrophotometric method로 분석(分析)한 결과(結果)는 다음과 같다. 1. 단백질분해효소(蛋白質分解酵素)를 0.01%, 0.05%, 0.1%로 첨가(添加)함에 따라 total tryptophan 과 free tryptophan은 계속 증가(增加)하였다. 2. Total tryptophan은 단백질분해효소(蛋白質分解酵素)를 육중량(肉重量)의 0.05% 첨가시(添加時) 그 증가율(增加率)이 가장 높았다. 3. 단백질분해효소(蛋白質分解酵素) 첨가(添加)에 따른 free tryptophan의 함량변화(含量變化)는 그다지 심하지 않았다.

• Ocean and Polar Research
• /
• 제30권4호
• /
• pp.467-472
• /
• 2008

Antarctic krill has a strong proteolytic enzyme system, which comes from a combination of several proteases. This powerful activity can be easily detected by krill's superior post mortem autolysis. Mammalian skin consists of epidermis and dermal connective tissue, and functions as a barrier against threatening environments. A clot in a wound site of the skin should be removed for successful skin regeneration. Epithelial cells secrete proteases to dissolve the clot. In previous studies Antarctic krill proteases were purified and characterized. The proteolytic enzymes from Antarctic krill showed higher activity than mammalian enzymes. It has been suggested that these krill clean up the necrotic skin wound to induce a natural healing ability. The enzymes exhibited additional possibilities for several other biomedical applications, including dental plaque controlling agent and healing agent for corneal alkali burn. Considering that these versatile activities come from a mixture of several enzymes, discovering other proteolytic enzymes could be another feasible way to enhance the activity if they can be used together with krill enzymes. Molecular cloning of the krill proteases should be carried out to study and develop the applications. This review introduces possible roles of the unique Antarctic krill proteases, with basic information and suggestion for the development of an application to skin regeneration.

• 한국식품영양과학회지
• /
• 제22권6호
• /
• pp.803-806
• /
• 1993

우리나라 전역의 산간지방에서 야생 또는 재배되는 닥나무의 열매는 옛날 가정에서 질긴 쇠고기를 조리할 때 사용하였다고 하므로 본 연구에서는 닥나무 열매를 육류조리에 이용하였을 때의 연화효과를 보고자하여 1차적으로 닥나무열매에서 추출한 단백질 분해효소의 활성도를 측정하였다. 시료의 효소를 조제하였을 때 시료추출물 100ml중의 회수된 조효소 분말량은 1.01g으로 회수율 6.2%를 나타내었고 단백질 분해효소 활성은 1.6 unit의 활성을 나타냈으며 닥나무 열매추출물의 단백질함량은 건물량으로 9.6%였다. 또한 효소의 기질에 대한 특이성은 casein 100에 egg white 50, hemoglobin 79, collagen 53, gelatin 42의 비율로 가수분해 하는 것으로 나타나 육류에 함유된 단백질인 hemoglobin과 collagen에 대한 효소의 가수분해율이 높은 것으로 나타났따. 효소의 최적온도는 $60^{\circ}C$로 관찰되었으며 열에 비교적 안정하였다. 효소의 최적 pH는 70이었으며, 전반적으로 넓은 pH영역에서 활성이 있는 것으로 나타났다.