• Asian-Australasian Journal of Animal Sciences
• /
• 제13권12호
• /
• pp.1743-1749
• /
• 2000

An experiment was conducted to investigate the effect of supplementation of commercial phytase and ${\beta}-glucanase$ to wheat-soybean meal based layer diets. Control (17% CP) and reduced protein (13.5% CP) diets were compared with and without phytase and/or ${\beta}-glucanase$. Reducing dietary crude protein levels reduced the amount of N excreted by laying hens with no adverse affect on egg production or overall feed conversion ratio. There was, however, a slight reduction in average egg weight. When phytase was added to the control protein diets it was possible to reduce the level of dicalcium phosphate in the diet without a loss in performance and daily P output was reduced significantly. When phytase was added to the reduced protein diets, however, there was a dramatic loss in performance in the last four weeks of the study. Supplementation of ${\beta}-glucanase$ to wheat based layer diet did not appear to have beneficial affects in terms of laying performance and reducing nitrogen or phosphorus excretion. Combination of phytase and ${\beta}-glucanase$ had no positive effects on laying performance or reduction of DM, N and P.

• 한국가금학회지
• /
• 제44권3호
• /
• pp.151-159
• /
• 2017

The experiment was carried out on 648 Ross 308 broilers ($40.4{\pm}0.2$ g; 1 d) chickens. It lasted for 28 days to evaluate the effects of phytogenic feed additive (PFA) on growth performance, nutrient digestibility, cecum microbiota, bone minerals contents, meat quality and internal organ weights. The chickens were haphazardly divided into any 1 of 6 dietary treatments (6 replicate pens/treatment; 18 broilers/pen). The experimental diets were: CON, control; T1, CON + 150 ppm PFA; T2, CP 0.5% reduced from basal diet; T3, T2 + 150 ppm PFA; T4, Ca 0.07% reduced + available P (aP) 0.065% reduced from basal diet; T5, T4 + 150 ppm PFA. Overall, broilers fed with T3 diet tended to have higher (P=0.08) body weight gain (BWG), and lower (P=0.05) feed conversion ratio (FCR) than T2 diet. The apparent total tract digestibility (ATTD) of P was lower (P=0.03) in T2 as compared to CON and it was higher (P=0.02) in T3 as compared to T2. There were no differences in meat quality, relative organ weight, and cecum microbiota (P>0.05). The supplementation of PFA showed trends in improvement in ash content in the bone of birds fed T1, T2 and T4 diets compared with CON. Likewise, the supplementation of PFA tended to increase (P=0.07) Ca content in the bone of birds fed T1 diet compared to CON. In conclusion, dietary supplementation with 150 ppm PFA could improve BWG in birds fed protein reduced diet, improve apparent total tract digestibility (ATTD) of P, and the bone Ca contents in broilers.

• Journal of Nutrition and Health
• /
• 제6권1호
• /
• pp.31-38
• /
• 1973

The effect of dietary condition on the acethylcholine and chotinesterase activity in the brain was observed. Rats weighting $120{\sim}130\;gm$ was fed on diets deprived of protein, carbohydrate and lipid, respectively. Experimental terms was devided into 3, 7 and 15 days. The results are summarized as follows: 1. The lipid free diet makes greater contribution to the gain of body weight than the carbohydrate free and protein free diet. 2. The acethylcholine in the protein free diet group is reduced after 7 days and that in the carbohydrate free diet, after 3 days. 3. The cholinesterase activity shows continuing decrease after 3 days by protein free diet, and the other diet groups appear temporal decrease at 7 days and increase, at 15 days. 4. The relation of acethylcholine, and cholinesterase is similar to each other except that of the carbohydrate diet. From the above results, the conclusions are obtained that the protein free diet makes the acethylcholine and cholinesterase activity lower, and the carbohydrate free diet, acethylcholine lower.

• 한국가금학회지
• /
• 제21권4호
• /
• pp.257-266
• /
• 1994

Three protein feeding systems for egg-type pullets involving conventional step-down protein 18-15-12%), step-up protein(12-15-18%) and single-stage low protein (13-13-13%) with an iso-energy level of 2,900 ME kcal /kg were compared to examine the effect on pullet growth and subsequent laying performance. During the growing period, pullets subjected to the step-up and single-stage low protein feeding systems were lighter in body weight and consumed less feed and netabolizable energy than those on the conventional step-down protein feeding system(P<0.05). 3ut the pullets on the step-up protein diet consumed more protein, and those on the single-stage low protein diet consumed less protein than those on the step-down protein diet(P<0.05). Also, he feed cost was less in pullets on the single-stage low protein diet than in those on the other systems(P<0.05). During the laying period, sexual maturity was later in hens reared on the step-up and single-stage low protein diets than in those on the step-down protein diet(P<0.05), however, average hen-day egg production and egg weight were not significantly affected by the protein feeding systems in the growing period. Daily feed intake and feed required per egg were significantly reduced in hens on the single-stage low protein diet compared to those on conventional protein feeding system(P<0.05). It was concluded that the 13% single-stage low protein feeding system produced smaller pullets with less feed, energy, protein, and feed cost during the growing period, and hens reared on that system consumed less feed during the laying period without any impairment of production compared to the conventional rearing system.

• Animal Bioscience
• /
• 제35권5호
• /
• pp.721-729
• /
• 2022

Objective: An experiment was conducted to determine the effects of supplementing graded concentrations of inorganic sulphur (S) without and with folic acid (FA) in maize-soybean meal diets on performance, slaughter and anti-oxidant variables, immune responses and serum protein fractions in broiler chicken. Methods: Inorganic S was supplemented at 0.05%, 0.10%, 0.15%, and 0.20% alone or in combination with FA (4 mg/kg) in basal diet (BD) containing no supplemental methionine (Met) and FA. A control group was fed with the recommended concentration of Met. Each diet was offered to 10 pens of 5 male broiler chicks (Cobb 400) and fed ad libitum from day 1 to 42. Results: The broilers fed the BD had lower body weight gain (BWG), feed efficiency (FE), higher lipid peroxidation (LP), lower activity of glutathione peroxidase (GSHPx), lower lymphocyte proliferation ratio (LPR), and reduced concentrations of total protein, albumin, and globulin in serum. Supplementation of FA and S to the BD improved the BWG (all concentrations of S) and FE (0.20% S) similar to the control group. Similarly, the combination of S and FA significantly improved the concentrations of total protein, albumin, and globulin in serum, reduced the LP and increased the activity of GSHPx and LPR. However, responses in the above parameters were related to the concentration of S in the diet. The slaughter variables and antibody titres against the Newcastle disease were not affected with the treatments. Conclusion: Based on the results, it is concluded that the combination of S (0.2%) and FA (4 mg/kg) improved the BWG and FE, similarly supplementation of these nutrients improved the concentration of protein fractions and reduced the stress (reduced LP and improved GSHPx) variables in serum and improved the cell mediated immune response (LPR) in broilers fed sub-optimal concentrations of Met in diet.

• 동아시아식생활학회지
• /
• 제25권1호
• /
• pp.111-120
• /
• 2015

This study was conducted to evaluate the effects of Sunsik prepared by steaming -drying cycles- on blood glucose and inflammatory markers in high fat diet-induced obese rats. The experimental animals were divided into five groups and subjected to diets for 8 weeks; normal diet (control), high fat diet (HF), and high fat diet+10% supplementation of Sunsik (1HF, 3HF, 7HF, 9HF). The results showed body weight, food efficiency ratio, blood glucose and insulin levels of high fat diet groups were significantly higher than those of the control group. Increased glucose levels by high fat diet were reduced to normal levels by the four Sunsik supplementations. Insulin resistance (HOMA-IR) of the HF group was significantly higher than those of the control group, whereas supplementation with the four types of Sunsik reduced insulin resistance to similar levels as the control group. Increased insulin secretion and leptin levels of high fat diet-induced obese rats were significantly reduced by supplementation with the four types of Sunsik. C-reactive protein (CRP) of inflammation related components was significantly higher than the control group. However, increased CRP concentration by high fat diet was significantly reduced by supplementation with Sunsik.

• Journal of Nutrition and Health
• /
• 제34권8호
• /
• pp.833-842
• /
• 2001

The purpose of this study is to determine the effect of dietary proteins and fats on the hepatic histological changes, membrane stability, and drug-metabolizing enzyme activities during chemically induced rat hepatocarcinogenesis. Weanling Sprague-Dawley rats were fed the diet containing 20% casein or soy protein isolate and 15% perilla or corn oil for 10 weeks. Hepatocarcinogensis was initiated with diethylnitrosamine(DEN), and the rats were fed diets containing 0.02% 2-acetylaminofluorene(AAF) followed by 0.05% phenobarbital (PB). The scores of histological changes were decreased in treated rats fed soy protein diet compared to those find casein diet. Liver weights were significantly increased by AAF and PB treatment in rats fed casein diets in both oil groups. Glucose 6-phosphatase(G6Pase) activities, an index of membrane stability, were significantly reduced by AAF and PB treatment in rats find casein diets, and were lower in casein diet compared to soy protein diet groups. Especially, the activities were the highest in the rats fed soy protein-perilla oil diet. Lipid peroxide values also were increased by AAF and PB treatment in rats fed casein diet. Aniline hydroxylase activities were not influenced by protein and fat sources. Glutathione-dependent enzyme activities were increased by AAF and PB treatment. Linoleic and arachidonic acid content were increased in rats fed corn oil diet, and linolenic and eicosapentaenoic acid contents were increased in rats fed perilla oil diet. Our results suggest that soy protein isolate inhibit the abnormal histological changes in liver, possibly by maintaining the membrane stability during chemically induced rat hepatocarcinogenesis. Soy protein may be protective against the hepatocarcinogenesis induced by chemical carcinogen.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권8호
• /
• pp.1147-1152
• /
• 2005

Two experiments were conducted to evaluate the effects of dietary Biotite V (BV) supplementation on growth performance, nutrients digestibility and fecal noxious gas content in finishing pigs. In Exp. 1, a total of eighty pigs (initial body weight 88.0${\pm}$1.35 kg) were used in a 35-d growth trial. Pigs were blocked by weight and allotted to five dietary treatments in a randomized complete block design. There were four pigs per pen and four pens per treatment. Dietary treatments included: 1) Control (CON; basal diet), 2) 200 mesh BV1.0 (basal diet+200 mesh Biotite V 1.0%), 3) 325 mesh BV1.0 (basal diet+325 mesh Biotite V 1.0%), 4) 200 mesh BV2.0 (basal diet+200 mesh Biotite V 2.0%) and 5) 325 mesh BV2.0 (basal diet+325 mesh Biotite V 2.0%). Through the entire experimental period, there were no significant differences in ADG, ADFI and gain/feed among the treatments (p>0.05). With the addition of Biotite V in diet, DM and N digestibilities were increased significantly (p<0.01). Also, Ca and P digestibilities tended to increase in pigs fed Biotite V supplemented diet (p<0.01) compared to pigs fed control diet. Supplementation of Biotite V in diet reduced the fecal $NH_3-N$ and volatile fatty acid (VFA) compared to CON treatment (p<0.01). In Exp. 2, a total of sixty four pigs (initial body weight 84.0${\pm}$1.05 kg) were used in a 35-d growth trial. Pigs were blocked by weight and allotted to four dietary treatments in a randomized complete block design. There were four pigs per pen and four pens per treatment. Dietary treatments included: 1) LP (low protein diet), 2) HP (high protein diet), 3) LP+BV (low protein diet+325 mesh Biotite V 1.0%) and 4) HP+BV (high protein diet+325 mesh Biotite V 1.0%). Through the entire experimental period, ADG and gain/feed tended to increase in HP and HP+BV treatments, however, there were no significant differences (p>0.05) among the treatments. With the addition of Biotite V in diets, digestibilities of nutrients (DM, N, Ca and P) were increased significantly (p<0.01). The addition of Biotite V in diets reduced the ammonia emissions in feces (p<0.01). Supplementation of Biotite V in diets also reduced the fecal propionic acid, butyric acid and acetic acid (p<0.01) compared to pigs fed diets without Biotite V. In conclusion, supplementation of Biotite V can increase nutrients digestibility and reduce fecal $NH_3-N$ and volatile fatty acid (VFA) concentrations in finishing pigs.

• Preventive Nutrition and Food Science
• /
• 제3권4호
• /
• pp.356-361
• /
• 1998

An experiment was performed to determine if buckwheat intake would improve insulin sensitivity in in normal healthy ras and steptozoticin-induced diabetic Sprague-Dauley rats. For four weeks, rats were fed either corn starch as a cotnrol diet or buckwheat as an experimental diet. As a result, the insulin sensitivity and plasma glucose levels in normal rats were not significantly affected by buckwheat fedding. The insulin sensitivity was lower in diabetic rats than in normal rats(p<0.05). Buckwheat tends to decrease the final plasma glucose level and increase insulin sensitivity in diabetic rats, but there was no sifnificant difference. Another five-week experiment was conducted to determine protein digestibility and protein utility in normal healty rats ad streptozotocin-induced diabetic rats on a control diet or buckwheat diet. The diet composition in this experiment was the same as the preceeding experiment. In the cotnrol diet groups, the protein digestibility in diabetic rats was significantly lower than that in normal rats(p<0.05). Buckwheat reduced protein digestibility in both normal and disbetic rats(p<0.05). Interestingly, in buckwheat diet groups, protei digestibility in diabetic rats was similar to that in normal rats. Protein utility was significantly lower indiabetic rats than in normal rats. This phenomenon was observed as early as the first week of the feeding period. However, protein utility was not sifnificanlty altered in both normal and diabetic rats by buckwheat feeding. It follows that decreased protein digestibility and utility in diabetic rts are not further aggravated by buckwheat feeding, suggesting that buckwheat can be a feasible supplement food for the diabetic therapeutic diet.

• Journal of Nutrition and Health
• /
• 제25권7호
• /
• pp.569-577
• /
• 1992

To study the effects of the age and the dietary protein content on Ca metabolism male rats of 1 month 6 month 12 month of age were fed experimental diets containing 5%, 15% or 50% casein for 4 weeks. Food and ca intake were higher in old rats and in high protein groups. The weight ash and Ca contents of femur and tibia were higher in old rats. The higher dietary protein level resulted in higher skeletal weigh ash and Ca contents. But high protein diet(50% casein) lead to reduced bone mineral density(ash/dry bone weight) and Ca density(Ca/dry bone weight) in 1 month old rats. Low protein diet(5% casein) on the other hand reduced the bone growth even though the bone density was higher in this group. The ill effect of low protein diet was not evident in 12 month old rats. Glomerular filteration rate(GFR) and urinary Ca excretionincreased with age and with dietary protein level especially in 12 month old rats. Serum immunoreactive parathyroid hormone(iPTH) level tended to be higher in aged rats but was not affected by dietary protein level except 1 month old rats where 50% protein group showed significantly higher value. This study showed that the dietary protein level seemed to have different effect on Ca metabo-lism in rats of different age., The low bone density in the high protein group of growing rats may be due to the higher iPTH level and increased urinary Ca. The dietary protein level however had no effects on the bone composition in aged rats even though the higher urinary Ca excretion. In conclusion this study suggests that high protein intake from young may lead to less peak bone mass and to increase the bone loss in later years, which would increase the risk for osteporosis.