• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.1
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• pp.21-26
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• 1990

This field experiment was carried out to determine the effects of nitrogen(N) application time (March 30, April 9 and April 19) and N fertilizer kind (urea and ammonium sulfate) in early spring on the grass growth, dry matter (DM) yield, crude protein (CP) content, CP yield (CPY), acid detergent fiber (ADF) content, DM digestibility (DMD) and N recovery in orchardgrass (Dactylis glomerata L.) meadow for soiling, 1989. The amount of N applied in early spring was 70 kg $ha^{-1}$, and non-fertilized plot was involved as control. In all fertilized plots the growth, DM yield, CP, CPY and regrowth yield of grasses were significantly increased compared with control. The best grass growth and DM yield were observed on March 30, and the best CP, CPY and N recovery were observed on April 9 among all fertilized plots. Also there were not significant differences between urea and ammonium sulfate fertilizer in grass growth, DM yield, CP, CPY, ADF, DMD and N recovery. In Suwon area, therefore, the optimum time of N application for soiling in early spring may be recommended early April, regardless of fertilizer kind of N.

• KSBB Journal
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• v.13 no.2
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• pp.133-140
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• 1998

Using rlFN-$\alpha$ and rhGH as the model proteins, the refolding performances of the published processes were evaluated and compared. Key engineering parameters such as the type of denaturant and this concentration, protein concentration in the refolding buffer, and pH and ionic strength of the buffer were experimentally investigated. Furthermore, the role of a co-solvent of surfactant type in aggregation reduction was also studied. Of the denaturants tested (8M urea, 6M guanidine HCI, 0.5% SDS), SDS at alkaline pH (9.5) and ambient temperature gave the highest recovery yield. The SDS process was effective in the refolding of observed where dissolution proceeded better under lower strength (10 mM) but aggregation was suppressed under higher strength (>50 mM.) When PEG-4000 and/or Tween were added as co-solvent or refolding-enhancing additive, 1.6-2 times higher yield was realized. The‘masking’of the hyrophobic patches located on the surface of the protein with the surfactant molecules was believed to be responsible for the considerable reduction in aggregation during refolding.

• Journal of Microbiology and Biotechnology
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• v.21 no.8
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• pp.869-873
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• 2011

Most medium formulations for improving culture of entomopathogenic nematodes (EPN) based on protein sources have used enriched media like animal feed such as dried egg yolk, lactalbumin, and liver extract, among other ingredients. Most results, however, showed unstable yields and longer production time. Many of the results do not show the detailed parameters of fermentation. Soy flour, cotton seed flour, corn gluten meal, casein powder, soytone, peptone, casein hydrolysates, and lactalbumin hydrolysate as protein sources were tested to determine the source to support optimal symbiotic bacteria and nematode growth. The protein hydrolysates selected did not improve bacterial cell mass compared with the yeast extract control, but soy flour was the best, showing 75.1% recovery and producing more bacterial cell number ($1.4{\times}10^9$/ml) than all other sources. The highest yield ($1.85{\times}10^5$ IJs/ml), yield coefficient ($1.67{\times}10^6$ IJs/g medium), and productivity ($1.32{\times}10^7$ IJs/l/day) were also achieved at enriched medium with soybean protein.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.719-722
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• 2001

Silk proteins from Nephila clavipes are fibrous proteins containing repetitive sequences with both crystalline and amorphous domains. In order to obtain high-level production of silk protein, the synthetic genes had 16 contiguous units of the consensus repeat sequence of the silk protein were expressed in Escherichia coli BL21(DE3) under the strong inducible T7 promoter. For production of recombinant silk protein in large amounts, pH-stat fed-batch cultures were carried out. The recombinant silk protein was produced as soluble forms in E. coli, and the recombinant silk protein content was as high as 11% of the total protein. When cells were induced at $OD_{600}$ of 60, the amount of silk protein produced was 6.49 g/L. After simple purification steps, 9.2 mg of silk protein that was more than 80% pure was obtained from a 50 mL culture, and the recovery yield was 26.3%.

• Journal of Microbiology
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• v.43 no.3
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• pp.295-300
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• 2005

In the present study, the performances of conventional purification methods, packed bed adsorption (PBA), and expanded bed adsorption (EBA) for the purification of the nucleocapsid protein (NP) of Newcastle disease virus (NDV) from Escherichia coli homogenates were evaluated. The conventional methods for the recovery of NP proteins involved multiple steps, such as centrifugation, precipitation, dialysis, and sucrose gradient ultracentrifugation. For the PBA, clarified feedstock was used for column loading, while in EBA, unclarified feedstock was used. Streamline chelating immobilized with $Ni^{2+}$ ion was used as an affinity ligand for both PBA and EBA. The final protein yield obtained in conventional and PBA methods was $1.26\%$ and $5.56\%$, respectively. It was demonstrated that EBA achieved the highest final protein yield of $9.6\%$ with a purification factor of 7. Additionally, the total processing time of the EBA process has been shortened by 8 times compared to that of the conventional method.

• Journal of the Korean Society of Food Culture
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• v.34 no.6
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• pp.779-784
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• 2019

The purpose of this study was to optimize the rice protein extracted using a response surface methodology. The experiment was designed based on a CCD (Central Composite Design), and the independent variables were the high pressure (X₁, 0-400 MPa) and processing time (X₂, 0-10 minutes). The results of the extraction content (Y₁), residue content (Y₂), and recovery yield (Y₃) were fitted to a response surface methodology model (R²= 0.92, 0.92, and 0.93, respectively). Increasing the pressure and processing time has a positive effect on the extraction content (Y₁), residue content (Y₂), and recovery yield (Y₃). Therefore, these high-pressure conditions (independent variables) can significantly affect the improvement in rice protein extraction efficiency. Thus, the optimal conditions of X₁ and X₂ were 400 MPa and 10 min., respectively. Under these optimal conditions, the predicted values of Y₁, Y₂, and Y₃ were 62.93, 57.53 mg/g, and 91.76%, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.1
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• pp.1-6
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• 1989

This field experiment was carried out to determine the effects of fertilizer application time in spring on the growth, crude protein(CP) content and dry matter(DM) yield of grasses for soiling. Application times of fertilizer were March 10, 20, 30, April 9 and 19, and control (non-fertilized) plot was involved. All fertilized plots were also treated with single- and compound fertilizer. The first harvesting date for soiling was May 12, and the regrowth soiling yield was investigated on June 9, 1988. In all fertilized plots, the growth, DM yield, CP, CP yield and regrowth yield of gasses were significantly increased compared with control, and the best grass growth, DM yield, CP, CP yield and N recovery were observed on March 30 and April 9 among all fertilized plots. And there were not significant differences between singleand compound fertilizer treatment in grass growth, DM yield and CP content. On March 30 and April 9 in this experiment, the accumulated temperature was 120 and 200^{\circ}C.$ and the subsoil temperature at 10 cm depth was 8 and 10^{\circ}C.$. Considering the average meteorological condition in Suwon area, the optimum application time of spring fertilizer for soiling may be recommended from April 3 to April 10.

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.3
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• pp.148-152
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• 1989

This field experiment was carried out to determine the effects of fertilizer application time in early spring on growth, dry matter (DM) yield, crude protein (CP) content, crude protein yield (CPY), N recovery, acid detergent fibej (ADF) content and DM digestibility (DMD) of grasses for hay. Application times of fertilizer were March 10, 20, 30, April 9. 19, and control (non-fertilized) plot was involved. The fust harvesting data for hay was May 9 (early heading stage) and the regrowth yields were investigated for soiling, 1989. In all fertilized plots, the growth, DM yield, CP, CPY and N recovery of grasses were significantly increased compared with control, and the best grass growth, DM yield, CP, CPY, N recovery and DMD were observed on March 20 and 30 among all fertilized plots. On March 20 and 30 in this experiment, the accumulated temperature was 200 and 280^{\circ}C.$, and the subsoil temperature at 10 cm depth was 8 and 10^{\circ}C.$. Considering the average meteorological condition in Suwon area, the optimum application time of early spring for hay may be recommended from April 10 to 15. i\ulcornerfW3U% (L. westock Experiment Station, RDA. Suwon 440 - 350. Korea)

• Journal of The Korean Society of Grassland and Forage Science
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• v.8 no.3
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• pp.141-146
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• 1988

This field experiment was carried out to determine the effects of fertilizer application time in spring on the growth, crude protein (CP) content and dry matter (DM) yield of gasses for grazing Application times of fertilizer were on March 10, March 20, March 30, April 9 and April 19, and control (non-fertilized) plot was involved. All fertilized plots were also treated with single and compound fertilizer. The first harvesting date for grazing was on April 30, and the regrowth soiling yield was investigated on June 9, 1988. In all fertilized plots, the growth, DM yield, CP, CP yield and nigrogen recovery of grasses were significantly increased compared with control, especially on the plot of March 30. The regrowth yield on March 30, April 9 and April 19 were shghtly hig!!a than those of the others. And there were not significant differences of grass growth, CP content, and DM yield between single-and compound fertilizer treatment. On March 30 which was the best time of fertilizer application in spring, the accumulated temperature was 116.6^{\circ}C.$ and the subsoil temperature at 10 cm depth was 8.1 - 8.6^{\circ}C.$. Considering the average meteorological condition in Suwon area, the optimum application time of spring fertilizer for grazing may be recommended on March 30-April 3 (accumulated temp.; 100-125^{\circ}C.$, subsoil temp.; 8-9^{\circ}C.$).

• Food Science of Animal Resources
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• v.39 no.3
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• pp.503-509
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• 2019

The aim of this study was to compare the effects of number of washes and salt addition on the meat quality in protein recovered from Alaska Pollock compared with pork leg. Various properties of protein recovered from Alaska Pollock (C, washed twice, no salt) and pork leg (T1, washed twice, no salt; T2, two washes, salt added; T3, washed four times, no salt; and T4, washed four times, salt added) were assessed in this study. Pork leg samples exhibited better color (more whiteness, less yellowness) than Alaska Pollock samples. In pork leg samples, four washes (T3, T4) during processing yielded whiter, less yellow protein than two washes (T1, T2). Overall, the textural property measures were higher in pork leg samples (T2, T3, and T4) than in other samples. Breaking force, jelly strength, and folding resistance were significantly higher in salt-treated pork leg samples (T2, T4) than in the other samples. Our findings demonstrate that protein recovered from pork leg has better color parameters, and physical strength compared with Alaska Pollock-derived protein. A higher number of wash steps and treatment with salt during processing were furthermore found to yield better color, and physical strength in the protein samples.