• Bulletin of the Korean Chemical Society
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• 제32권8호
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• pp.2732-2736
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• 2011

In pH 4.2 HAc-NaAc buffer solution, horseradish peroxidase (HRP) catalyzed $H_2O_2$ oxidation of nanosilver to form $Ag^+$. After centrifugation, $Ag^+$ in the supernatant can be measured by graphite furnace atomic absorption spectrophotometry (GFAAS) at the silver absorption wavelength of 328.1 nm. When HRP concentration increased, the $Ag^+$ concentration in the supernatant increased, and the absorption value enhanced. The HRP concentration in the range of 0.84-50 $ng{\cdot}mL^{-1}$ was linear to the enhanced absorption value (${\Delta}A$), with a regression equation of ${\Delta}A$=0.012C+0.11, correlation coefficient of 0.9988, and detection limit of 0.41 $ng{\cdot}mL^{-1}$ HRP. The proposed GFAAS method was used to detect HRP in waste water samples, with satisfactory results.

• The Plant Pathology Journal
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• 제33권1호
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• pp.21-33
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• 2017

Citrus blast caused by bacterium Pseudomonas syringae is a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. Considering the above fact, this study reports genetic characterization of 43 P. syringae isolates obtained from plant tissue displaying citrus blast symptoms on mandarin (Citrus reticulata) in Montenegro, using multilocus sequence analysis of gyrB, rpoD, and gap1 gene sequences. Gene sequences from a collection of 54 reference pathotype strains of P. syringae from the Plant Associated and Environmental Microbes Database (PAMDB) was used to establish a genetic relationship with our isolates obtained from mandarin. Phylogenetic analyses of gyrB, rpoD, and gap1 gene sequences showed that P. syringae pv. syringae causes citrus blast in mandarin in Montenegro, and belongs to genomospecies 1. Genetic homogeneity of isolates suggested that the Montenegrian population might be clonal which indicates a possible common source of infection. These findings may assist in further epidemiological studies of this pathogen and for determining mandarin breeding strategies for P. syringae control.

• The Plant Pathology Journal
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• 제37권6호
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• pp.512-520
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• 2021

In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.

• 한국물환경학회지
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• 제24권5호
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• pp.592-597
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• 2008

Current water quality standard (WQS) in Korea is based on the protection of human health, not considering the protection of aquatic organisms. Most of chemicals can be toxic to ecological biota as well as human. Health of aquatic biota is closely related to the human health via food chain, therefore ecological risk based-WQS needs to be developed to protect the aquatic ecosystem. In this study, we selected the 31 chemicals in the Project entitled 'Development of integrated methodology for evaluation of water environment'. The methodology for calculating water quality criteria was derived from the Australian and New Zealand processes for deriving guideline trigger value for aquatic ecosystem. The available ecotoxicity data were collected from US EPA's ECOTOXicology Database (ECOTOX), TOX-2000 Database, European Chemicals Bureau (ECB)'s International Uniform Chemical Information Database (IUCLID) and Environmental Protection Agency (US EPA)'s report 'Ambient Water Quality Criteria (AWQC)'. The aquatic toxicity data for the Korean species were selected for risk assessment to reflect the Korean water environment. The monitoring values were calculated from the water quality monitoring data four main Korean rivers. We suggested the order of priorities of chemicals based on ecological risk assessment. We expect that these results can be useful information for establishing the WQS for the protection of aquatic ecosystem.

• Journal of Electrochemical Science and Technology
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• 제14권3호
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• pp.205-214
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• 2023

Soil salinity is becoming one of the most devastating environmental hazards over the years. Soil investigation involves fast, low cost and non disturbing methods to measure soil characteristics for both construction projects as well as for agricultural use. The electrical resistivity of saline soils is greatly governed by salt concentration and the presence of moisture in soil matrix. Experimental results of this investigation highlight that there is a significant relationship between the electrical resistivity of soil samples mixed with chloride solutions (NaCl, KCl, and MgCl₂) at various concentrations, and soil physical properties. Correlations represented by quadratic functions were obtained between electrical resistivity and soil characteristics, namely, water content, degree of saturation and salt concentration. This research reveals that the obtained correlations between electrical resistivity, salt concentration, water content and degree of saturation are effective for predicting the characteristics of salt affected soils in practice, which constitute a governing element in the assessment of saline lands sustaining infrastructure.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2002년도 총회 및 춘계학술발표회
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• pp.159-162
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• 2002

Innovative and nondestructive characterization techniques have been developed to locate and quantify nonaqueous phase liquids (NAPLs) in the vadose and saturated zones in the subsurface environment. One such technique is the partitioning interwell tracer test (PITT). The PITT is a simultaneous displacement of partitioning and non-partitioning tracers through a subsurface formation. Partitioning tracers will partition into the NAPL during their transport through NAPL-contaminated formations. Mean travel times of partitioning and non-partitioning tracers are used to estimate the quantity of NAPL encountered by the displaced tracer pulse. Travel times are directly proportional to the partitioning coefficient and the volume of NAPL contacted in the subsurface environment. This paper discusses the conceptual background, design and implementation of PITTs. (This document has not been subjected to Agency review and therefore does not necessarily reflect the views of the Agency, and no official endorsement should be inferred.)

• 중환자간호학회지
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• 제13권1호
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• pp.15-26
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• 2020

Purpose : This study aimed to examine factors affecting radiation protective behaviors among emergency room nurses by assessing knowledge, attitude, and environment for radiation protection. Methods : The study employed a cross-sectional design. Subjects were a convenience sample of 129 nurses working in emergency rooms of three general hospitals. Data were collected using self-report questionnaires and analyzed using t-test, ANOVA, Pearson correlation coefficients, and multiple regression. Results : The more the nurses received radiation safety education (t=2.26, p=.026), used protective gear (t=4.40, p<.001), and took health screenings (t=2.65, p=.009), the higher their levels of radiation protection behavior. There were significant relations between radiation protective behaviors and attitude (r=.27, p=.002), and radiation protective behaviors and environment for radiation protection (r=.55, p<.001). The factors affecting radiation protective behaviors were protective environment (β=.53, p<.001), protective attitude (β=.32, p<.001), and the use of protective gear (β=.24, p=.002). Conclusions : The government, hospital administrators, and radiation protection-related organization should adopt the following measures to protect emergency room nurses from radiation: research and development of shield instrument, medical examination for emergency room nurses, protocol development of radiation protection behaviors, extension of education chances of radiation protection, and encouraging the use of protective equipment.

• 환경위생공학
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• 제1권1호
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• pp.47-58
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• 1986

The underground living spaces have become one of the major environment of this decade in urban area. This study was carried out to examine contamination level for purpose of preservation of the pleasant underground environment. Three subway stations and three underground shopping centers in Seoul and two underground shopping centers in Busan were selected and surveyed by measuring gaseous pollutants $(SO_2,\; NO_2,\;CO,\;HC,\; HCHO,\;CO_2)$, dust, airborne microbes, and the other air condtions (temperature, humidity, air pressure, air flow, kata cooling power). These examined data were compared with four kinds of standard (building sanitation management, room air quality standards, occupational safety and health standards, ventilation equipment) as environmental hygiene.

• 한국산림과학회지
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• 제111권1호
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• pp.72-80
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• 2022

본 연구는 백두대간보호구역에 대한 합리적인 보전 및 이용을 위한 권역별 지향성 설정을 위하여 실시하였다. 지향성 설정을 위해 첫 번째 단계로 자연환경보호, 자연경관보호, 농림업 진흥, 생활환경보호, 교육·문화·예술 진흥, 휴양·관광 진흥, 재난·재해관리 유형을 백두대간의 설악산, 태백산, 속리산 권역을 포함하는 유역별 지향성의 점유비율을 비교하여 결정하였다. 그러나, 7가지 지향성별로 각각의 유형이 대상지역에서 동일한 역할을 하지 않기 때문에 가중치를 적용하였다. 그 결과 백두대간 지역은 산림 및 환경보호에 대한 강력한 조치로 규제되고 있으며, 자연보호와 관련된 가중치가 다른 지역보다 높기 때문에 대부분의 지역이 자연환경보호 구역으로 분류되었다. 이상의 결과를 보완하기 위해서는 권역별 지향성과 가중치를 결정하기 위한 추가 연구 수행과 사회문화 요소 및 현장 조사 자료의 통합이 필요할 것으로 판단된다.

• Journal of Radiation Protection and Research
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• 제23권3호
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• pp.197-210
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• 1998

IAEA는 1996년 방사성물질 안전 운송규정을 개정하였다. 이 규정은 방사성물질의 우송이나 포장에 관하여 우리나라를 비롯한 각국의 운송관련 규정의 기준이 되는 것으로 전반부에서는 IAEA가 1991년에 출간된 국제방사선방호위원회 (ICRP)의 신권고(Publication 60)를 받아들여 개정한 Safety Series No.115(전리방사선에 대한 방호 또는 방사선원의 안전을 위한 기본 안전기준)의 내용 등을 개정의 배경으로 하여 요약하였다. 후반부에서는 이들 개정된 기본 안전 기준들에 기초하여 IAEA의 새로운 운송규정에서 방사선방호의 목적으로 고찰된 요건들에 관한 주요 검토 개정사항을 방사선방어체계, 운송물등의 방사선준위, 방사선방호계획의 규정화, Q 시스템의 개념, 규제면제 등의 측면에서 Safety Series No.6 1985년판과 비교 검토하였다.