• 제목/요약/키워드: protected culture

검색결과 202건 처리시간 0.032초

열수지 해석에 의한 온실 수경재배 작물의 증산속도 추정에 관한 연구 (The Estimation of Transpiration Rate of Crops in Hydroponic Culture in the Plastic Greenhouse)

  • 남상운;김문기
    • 태양에너지
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    • 제10권3호
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    • pp.27-34
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    • 1990
  • 온실 수경재배 작물의 증산속도와 환경요인과의 관계를 규명할 목적으로 플라스틱 온실 내부 작물의 엽면 열수지식을 해석하여 온실 내부의 환경요인에 따른 엽온 및 증산속도를 추정할 수 있는 컴퓨터 모형을 개발하였다. 여기서 개발된 모형은 실제 플라스틱 온실에서 실시한 상추의 수경재배 실험 결과를 이용하여 검증하였으며, 앞으로 다른 작물에 대한 연구가 보완된다면 이 모형을 이용하여 시설재배 작물의 물관리 연구 및 열환경 분석에 유용하게 적용할 수 있을 것으로 사료된다.

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영지 액체 배양의 Wall Growth에 미치는 Polyacrylic Acid의 첨가 효과 (Effect of Polyacrylic Acid Addition on Wall Growth in Submerged Cultivation of Ganoderma lucidum)

  • 이신영;이학수
    • 산업기술연구
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    • 제21권A호
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    • pp.337-341
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    • 2001
  • This study was carried out to screen the effective polymeric additives preventing wall growth during mycelial submerged cultivation of Ganoderma. lucidum. Effects of additives on mycelial growth and exo-polysaccharide (EPS) production in flask culture and jar fermenter system under 3 different pH processes were investigated, and changes of mycelial morphology were also examined. From flask culture of G. lucidum with additives of different concentrations, 0.1%(w/v) polyacrylic acid was effective for EPS production. As the polyacrylic acid of 0.1%(w/v) was added in medium, wall growth of G. lucidum mycelium grown in jar fermenter system could be protected. The addition of 0.1%(w/v) polyacrylic acid to medium was also improved the mycelial growth and EPS production in the later of submerged culture G. lucidum and no changes of mycelial morphology were observed.

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지경건축여경상공간(地景建筑与景象空間) - 화청지풍경구경관문화유산적개치전시(華淸池風景區景觀文化遺産的价値展示) (Chinese Landscape Architecture and View-Shedspace - Values' Presentation of the Landscape Heritage of the Huaqing Palace Scenery Area -)

  • 유휘;동유철
    • 한국전통조경학회지
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    • 제28권4호
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    • pp.98-104
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    • 2010
  • Chang'an, an ancient historical city with its construction basement remains. Chinese Traditional Landscape Culture comes from the ancient civilization of the Yellow River and the environment of the central Shaanxi plain forms its ideology characteristic. Basing on the fast urbanization and growth of economies, there is a growing emphasis on Urban Landscape Cultural Heritage. The spatial pattern and traditional landscape should be protected and exhibit because it reflects the original characteristic and implied meaning of Chinese traditional landscape culture. This paper presents the case of Huaqing Palace(華淸宮) scenery area as an example; evaluate the value of its original landscape culture, showing the way of the conservation and regulation.

Hepatoprotective Effect of Stamen Extracts of Mesua ferrea L. against Oxidative Stress induced by $CCl_4$ in Liver Slice Culture Model

  • Rajopadhye, Anagha A.;Upadhye, Anuradha S.
    • Natural Product Sciences
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    • 제18권2호
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    • pp.76-82
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    • 2012
  • Stamens of Mesua ferrea L. are a well-known herbal drug used in Indian System of Traditional Medicine to treat various diseases. The claimed activity of this plant part is necessitated to investigate antioxidant and hepatoprotective activity. Authenticated plant sample was extracted with hexane, ethanol (EtOH) and water (aq.) using ASE 100 accelerated solvent extractor. Antioxidant activity was evaluated by means of different in vitro assays. Hepatoprotective effect was investigated on carbon tetrachloride induced oxidative stress in liver slice culture model. Cytotoxic marker lactate dehydrogenase (LDH) released in culture medium and the activity of lipid peroxidation along with antioxidant enzymes (AOEs) namely superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were estimated. Hexane and EtOH extracts were significantly inhibited DPPH, NO, SOD and $ABTS^+$ radical in dose dependent manner. The trade of phenol content was: aq. extract < hexane extract < EtOH extract. A significant correlation was shown by total phenol content and free radical scavenging activity of extracts. The culture system treated with hexane extract, EtOH extract or ascorbic acid exhibited significant depletion in LDH, lipid peroxidation, antioxidative enzymes SOD, CAT and GR. Hexane extract and EtOH extracts of stamen of M. ferrea protected liver slice culture cells by alleviating oxidative stress induced damage to liver cells.

Thinking multiculturality in the age of hybrid threats: Converging cyber and physical security in Akkuyu nuclear power plant

  • Bicakci, A. Salih;Evren, Ayhan Gucuyener
    • Nuclear Engineering and Technology
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    • 제54권7호
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    • pp.2467-2474
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    • 2022
  • Nuclear Power Plants (NPPs) are the most protected facilities among all critical infrastructures (CIs). In addition to physical security, cyber security becomes a significant concern for NPPs since swift digitalization and overreliance on computer-based systems in the facility operations transformed NPPs into targets for cyber/physical attacks. Despite technical competencies, humans are still the central component of a resilient NPP to develop an effective nuclear security culture. Turkey is one of the newcomers in the nuclear energy industry, and Turkish Akkuyu NPP has a unique model owned by an international consortium. Since Turkey has limited experience in nuclear energy industry, specific multinational and multicultural characteristics of Turkish Akkuyu NPP also requires further research in terms of the Facility's prospective nuclear security. Yet, the link between "national cultures" and "nuclear security" is underestimated in nuclear security studies. By relying on Hofstede's national culture framework, our research aims to address this gap and explore possible implications of cross-national cultural differences on nuclear security. To cope with security challenges in the age of hybrid threats, we propose a security management model which addresses the need for cyber-physical security integration to cultivate a robust nuclear security culture in a multicultural working environment.

보호수 지정 및 관리를 위한 남해안 지역 산림문화자산 실태조사 (A Survey on the Forest Cultural Assets in the Southern Coastal Areas for the Designation and Maintenance of Law-protected Trees)

  • 강기래
    • 한국전통조경학회지
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    • 제30권2호
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    • pp.28-36
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    • 2012
  • 산림은 우리 생활 가까이에서 의복과 문화, 생존의 기반을 제공해 주고 있는 기본적인 자산이다. 이러한 자산은 문화와 생활로서 지역 주민들의 삶 속에 녹아들어 있으며, 후세로 이어져 나갈 것이다. 본 연구는 산림문화자산에 대한 조사가 부족한 지역인 남해안지역의 노거수 및 군락지와 숲에 대한 현지조사를 실시하여, 보호수로서의 지정가능성을 검토하기 위해 수행되었다. 현장 조사는 남해안에 접해 있는 경남지방 전남지방의 11개 시, 군에 위치한 노거수 및 군락지와 숲 등 80개소를 조사하였다. 노거수 및 군락지와 숲의 건강성과 관리상태를 비교적 객관적으로 측정하기 위한 판단기준을 전문가 표적토론(brainstorming)을 통하여 설정하였다. 현장 조사 결과, 현재 보호수로 미지정된 노거수 및 군락지와 숲 중에서 산림청 예규 제 574호의 기준에 의한 법정보호수 지정 기준을 통과하고, 관리상태도 좋은 노거수 및 군락지와 숲을 선정하였다. 군락지는 남해 난음리 비자나무 숲이 선정가능성이 높으며, 노거수는 하동 평사리 부부소나무를 포함한 13개소의 나무들이 지정 가능성이 높은 것으로 판단하였다. 노거수의 수종은 느티나무 3주, 팽나무 3주, 소나무 2주, 모밀잣밤나무, 왕버들, 후박나무 각 1그루씩이었다. 이러한 노거수와 군락지는 보호수로 지정하여서 국가의 체계적인 관리를 필요로 하며, 우리의 산림문화자산으로서 그 가치를 이어가야할 것으로 판단한다.

Production of ${\gamma}-Aminobutyric$ Acid (GABA) by Lactobacillus buchneri Isolated from Kimchi and its Neuroprotective Effect on Neuronal Cells

  • Cho, Yu-Ran;Chang, Ji-Yoon;Chang, Hae-Choon
    • Journal of Microbiology and Biotechnology
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    • 제17권1호
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    • pp.104-109
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    • 2007
  • Lactic acid bacteria that accumulated ${\gamma}-aminobutyric$ acid (GABA) in culture medium were screened to identify strains with high GAB A-producing ability. One strain, MS, which was isolated from kimchi, showed the highest GABA-producing ability among the screened strains. MS was identified as Lactobacillus buchneri based on Gram-staining, metabolic characteristics, and 16S rDNA sequence determination, Optimum culture conditions for GABA production were determined: MRS broth containing 5% MSG, 1% NaCl, and 1% glucose, at an initial pH of 5.0, the incubation temperature at $30^{\circ}C$ for 36 h. Under these conditions, MS produced GABA at a concentration of 251 mM with a 94% GABA conversion rate. Moreover, culture extracts of Lb. buchneri MS partially or completely protected neuronal cells against neurotoxicantinduced cell death.

Development of a toxA Gene Knock-out Mutant of Pasteurella multocida and Evaluation of its Protective Effects

  • Kim Tae-Jung;Lee Jae-Il;Lee Bong-Joo
    • Journal of Microbiology
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    • 제44권3호
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    • pp.320-326
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    • 2006
  • Pasteurella multocida is an important veterinary and opportunistic human pathogen. In particular, strains of P. multocida serogroup D cause progressive atrophic rhinitis, and produce a potent, intracellular, mitogenic toxin known as P. multocida toxin (PMT), which is encoded by the toxA gene. To further investigate the toxigenic and pathogenic effects of PMT, a toxA-deleted mutant was developed by homologous gene recombination. When administrated to mice, the toxigenicity of the toxA mutant P. multocida was drastically reduced, suggesting that the PMT constributes the major part of the toxigenicity of P, multocida. Similar results were obtained in a subsequent experiment, while high mortalities were observed when toxA(+) P. multocida bacterial culture or culture Iysate were administrated. Mice immunized with toxA(-) P. multocida were not protected (none survived) following challenge with toxA(+) P. multocida or bacterial culture Iysate (toxin). These results suggest that the toxigenicity of P. multocida is mainly derived from PMT.

EFFECT OF DITHIOL MALONATE DERIVATIVES (DMDs) ON CARBON TETRACHLORIDE-INDUCED HEPATOTOXICITY IN PRIMARY CULTURES OF ADULT RAT HEPATOCYTES

  • Jung, Hyun-Ho;Jeong, Tae-Cheon;Yang, Kyu-Hwan;Chun, Young-Jin
    • Toxicological Research
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    • 제9권2호
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    • pp.167-175
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    • 1993
  • Protective effects of dithiol malonate derivatives (DMDs), YH-100, YH-150 and YH-439 on carbon tetrachloride-induced hepatotoxicity were investigated in primary rat hepatocytes culture. Treatment of DMDs to hepatocytes culture did not affect total cytochrome P-450 content and ECOD and AHH activities. Protein and RNA synthesis was also similar to control. Meanwhile, DMDs significantly decreased LDH release and in vitro lipid peroxidation induced by $CCI_4$. Accumulation of cellular triglyceride and decreased secretion of VLDL from liver cells by $CCI_4$ treatment were also significantly protected.

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기관 삽관 중인 환자에서 Blind Protected Specimen Brushing의 역할 (The Role of Blind Protected Specimen Brushing (PSB) in Intubated Patients)

  • 유희승;홍지현;윤장욱;엄광석;이재명;김철홍;장승훈;김동규;이명구;현인규;정기석
    • Tuberculosis and Respiratory Diseases
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    • 제55권1호
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    • pp.59-68
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    • 2003
  • 연구배경 : 기관 삽관을 한 환자에서 정확한 폐렴의 진단과 원인균을 파악하는 것이 쉽지 않다. 경기관 흡인물(endotracheal aspirate) 배양검사는 간편하지만, 객담채취시 오염의 기회가 많고, 부적절한 객담채취의 기회가 높아서 그 원인균을 알아내는데는 어려움이 많다. 따라서 본 연구에서는 폐렴 진단 방법의 일환으로 좀 더 간편하고 비침습적인 방법인 blind PSB를 시행하여 진단의 유용성과 치료 및 예후에 미치는 영향을 알아보고자 하였다. 방 법 : 기관 삽관을 한 환자중에서 감염성 폐령이 의심되었던 51명의 환자를 대상으로 blind PSB(protected specimen brushing)를 시행하였고, 경기관 흡인물(endotracheal aspirate) 배양 및 혈액 배양검사를 통한 진단 결과와 비교하였다. 대상환자에서 기관 삽관 튜브를 통하여 폐렴이 생긴 폐구역에 해당되는 기관지에 접근하여 PSB 카테터로 기관지내의 화농성 분비물을 채취하여 15분 이내에 정량적 배양검사를 의뢰하여 $10^3/ml$ 이상의 colony forming units를 보일 때 양성으로 판정하였다. 결 과 : 51명의 환자 중 병원내 감염 폐렴은 27명(52.9%)이었고, 이 중에서 VAP가 19명(37.3%)이었고, 지역사회 감염 폐렴은 15명(29.4%)이었다. 비 폐렴성 폐 침윤으로 확인된 9명(17.6%)의 환자 중 울혈성 심부전이 5명(98%), 폐결핵이 3명(5.9%), 폐출혈이 1명(2.0%) 이었다. 원인균 규명을 위한 배양검사의 민감도와 특이도는 객담에서 각각 83.3%(35/42), 77.8%(7/9), 혈액에서 각각 14.3%(6/42), 100%(9/9)였고, blind PSB에서는 각각 548%(23142), 88.9%(8/9)였다. 유사한 민감도와 특이도를 고려하면, blind PSB는 객담배양검사에 비하여 우월하다고 할 수 있다. 초기 항생제 치료는 blind PSB 배양결과에 따라서 항생제를 교체하였는데, 항생제를 교체한 환자군의 생존율은 교체하지 않았던 환자군의 생존율과 통계적으로 유의한 차이가 없었다. 결 론 : Blind PSB는 기관 삽관된 환자에서 폐렴이 의심되는 경우 비감염성 폐침윤과의 감별에 도움을 주고 폐렴의 원인균을 간편하고 빠르게 확인하는데 유용하다. 또한 경기관 흡인물 배양검사에 비해 신뢰도는 낮지 않으면서 정량적 배양이 가능하여 불필요한 항균제의 사용을 감소시킬 수 있을 것으로 사료되며, 기관지 내시경검사를 할 수 없는 환자에서도 이용이 가능하다. 또한 기관지 내시경이 필요없어 검사비용에 비한 효과면에서 유용하고 안전한 검사이다.