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Study of Structure Change by Temperature Effect in Spin Label of Myosin Head (Myosin Head의 Spin Label이 온도 영향에 따른 구조 변화 연구)

  • Kim, Duck-Sool;Park, Keun-Ho
    • Journal of the Korean Applied Science and Technology
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    • v.20 no.3
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    • pp.268-273
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    • 2003
  • IASL(iodo acetamide spin label) and MSL(maleimide spin label) disordered the orderly helix arrangement of myosin in the rest state of spin level. Especially the effect of IASL was great. The muscle was isometrically tetanized with three trains of 3ms pulses every 50ms between $5^{\circ}C$ with $25^{\circ}C$. Equatorial reflection change inferred that myosin head was moved to the vicinity of actin filament by spin level. The intensity change of $143{\AA}$ and $72{\AA}$ could offer information of the mass projection of population of myosin head along the filament axis. The slope of intensity profile of the mass projection of $143{\AA}$ and reflection of IASL is appeared and that of MSL is appeared sharply. The decrease of $215{\AA}$ reflection intensity the periodical character of $143{\AA}$ reflection by spin label. The raise of MSL actin reflection at $51{\AA}$ and $59{\AA}$ in the actin reflection change refers that the shifted myosin head binds a certain actin or changes an actin structure by spin label effect. Because iodo acetamide has a tendency to decease the actin reflection, actin dose not bind myosin head. From this result, we can conclude that IASL and MSL are spin labeled on SH of myosin head and disordered the helix arrangement of actin.

Studies on the Ecological Change of the Plant Community in the Erosion-Controlled and Rehabilitated Areas - During 9~26 Years After Erosion Control Works - (사방시공지 식물사회의 생태학적 변화에 관한 연구(V) - 사방시공 후 9~26년 간의 변화 -)

  • Lee, Hyun-Kyu
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.6 no.5
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    • pp.59-69
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    • 2003
  • Most denuded mountain areas in Korea were completely stabilized by the successful work of the 1st and 2nd 10-year Forest Development Plans which targeted the reforestation of denuded forest lands. The objectives of this study are (1) to estimate the depth of organic horizon in the soil profile, (2) to investigate the change of vegetation structure, (3) to estimate the change of biomass in the erosion controlled and rehabilitated mountain areas with the passage of time. This study was carried out as the 5th times. The first study began in the year of 1985, the second study was in the year of 1988, the third study was in the year of 1992, 4th was in the year of 1998 and 5th was in the year of 2002. The first study started in the study sites which elapsed 9 years after erosion control works. The results of the study were as follows : The increase rate of soil thickness was estimated to $Y_{(cm)}=2.906log_{(yr)}-3.2476(r^2=0.917)$ during 26 years after erosion control works. The important value of pines decreased to 14.7% on upper layer. But, the important value of alders. which did not plant on erosion control work increased to 27.1%. The decrease of whole crown projection indicates that pines. and alders were heavily injured by pine leaf gall midge in the year of 1993, 1995 years and Agelastica coerulea Baly in the year of 1986, 1987 years at Yoju-gun. The young growth of pines and alders not appeared on the soil surface which elapsed 26 years after erosion control works. On the lower layer, oaks occupied over 50% in I.V, RD, RC, RF. In process of years, the increase of biomass estimated to be $Y_{(t/ha)}={0.7505X_{(yr)}}^{1.6335}\;(r^2=0.9712)$ for 26 years after erosion control works.

Quality Characteristics of Frozen Stored Mungbean Starch Gels Added with Sucrose Fatty Acid Ester

  • Choi, Eun-Jung;Oh, Myung-Suk
    • Food Quality and Culture
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    • v.3 no.2
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    • pp.53-58
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    • 2009
  • This study was conducted to investigate the quality characteristics of frozen stored mungbean starch gels added with sucrose fatty acid ester (SE). The study showed a delay of gelatinization of mungbean starch by SE addition through the measurements conducted by using Rapid Visco Analyzer (RVA) and Differential Scanning Calorimeter (DSC). In the color of SE added frozen stored gels, lightness (L) and yellowness (b) values were increased compared to those of values measured from freshly prepared gel, whereas redness (a) value was decreased. The addition of 1% SE on mungbean starch gel prevented the color change during frozen storage. Rupture stress and rupture energy of frozen stored gel was higher than those of freshly prepared gel, whereas rupture strain of frozen stored gel was lower than that of freshly prepared gel. The addition of 1% SE on mungbean starch gel prevented the change of rupture characteristics during frozen storage. Texture profile analysis(TPA) characteristics revealed a significant change of the gel texture during frozen storage by showing an increase of hardness of the frozen stored gels compared to the freshly prepared gels with newly discovered fracturability, which resulted to show a large difference of gel texture by showing the disappearance of adhesiveness and large reduction of cohesivenes. The addition of 1% SE on mungbean starch gel prevented the change of TPA characteristics during frozen storage. Scanning electron micrographs showed that network structure of frozen stored gel was more rough than that of freshly prepared gel, and the addition of 1% SE on mungbean starch gel could suppress the breakdown of network structure. Thus the addition of 1.0% SE on mungbean starch gel was appropriate method for remaining gel characteristics during frozen storage.

Study on texture change of cooked rice within a short time after cooking (취반 후 단시간 경과에 의한 쌀밥의 조직감 변화 연구)

  • Shin, Sun-Hwa;Choi, Won-Seok
    • Korean Journal of Food Science and Technology
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    • v.52 no.3
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    • pp.310-315
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    • 2020
  • The aim of this study was to investigate the texture change of cooked rice within a short time after cooking. Using four instant rice brands, the texture change within 30 min after cooking was measured by the texture profile analysis (TPA) method for hardness, adhesiveness, cohesiveness, chewiness, and springiness with different compression ratios (30, 70%) and cross-head speeds (0.5, 1.0 mm/s). In the case of cohesiveness, adhesiveness, and chewiness, there were significant differences in the rice textures at 20 to 30 min after cooking compared to that in the sample immediately after cooking. In particular, adhesiveness showed significant differences at 10 min after cooking. However, there were little significant differences within 30 min for springiness. In conclusion, when measuring cooked rice texture, it is desirable to measure it, if possible, within 10 to 20 min after cooking.

Long-Term Trend of Surface Wind Speed in Korea: Physical and Statistical Homogenizations (한반도 지상 풍속의 장기 추세 추정: 관측 자료의 물리적 및 통계적 보정)

  • Choi, Yeong-Ju;Park, Chang-Hyun;Son, Seok-Woo;Kim, Hye-Jin
    • Atmosphere
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    • v.31 no.5
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    • pp.553-562
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    • 2021
  • The long-term trend of surface wind speed in Korea is estimated by correcting wind measurements at 29 KMA weather stations from 1985 to 2019 with physical and statistical homogenization. The anemometer height changes at each station are first adjusted by applying physical homogenization using the power-law wind profile. The statistical homogenization is then applied to the adjusted data. A standard normal homogeneity test (SNHT) is particularly utilized. Approximately 40% of inhomogeneities detected by the SNHT match with the sea-level-height change of each station, indicating that an SNHT is an effective technique for reconciling data inhomogeneity. The long-term trends are compared with homogenized data. Statistically significant negative trends are observed along the coast, while insignificant trends are dominant inland. The mean trend, averaged over all stations, is -0.03 ± 0.07 m s-1 decade-1. This insignificant trend is due to a trend change across 2001. A decreasing trend of -0.10 m s-1 decade-1 reverses to an increasing trend of 0.03 m s-1 decade-1 from 2001. This trend change is consistent with mid-latitude wind change in the Northern hemisphere, indicating that the long-term trend of surface wind speed in Korea is partly determined by large-scale atmospheric circulation.

A CEPHALOMETRIC STUDY OF PROFILE CHANGES FOLLOWING ORTHOGNATHIC SURGERY IN PATIENTS WITH MANDIBULAR PROGNATHISM (하악전돌증환자의 악교정수술후 안면측모 변화에 관한 두부방사선 계측학적 연구)

  • Lee, Hyung Sik;Park, Yung Chael
    • The korean journal of orthodontics
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    • v.17 no.2
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    • pp.299-310
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    • 1987
  • The purpose of this study was to examine soft tissue and hardtissue changes following orthognathic surgery in patients with mandibular prognathism lateral cephalometric films were obtained immediate before surgery, 48 hours following surgery, and 6 months following surgery. 18 patients were selected (10 men, and 6 women) for this study, who had received orthognathic surgery. Statistical analysis for the each time interval differences were performed with the SPSS package The results were as follows, *In the cases of mandibular sagittal split osteotomy 1 LI point was moved backward (average 7.55mm) 48 hours following surgery. 6 months later, it was returned forward (average 1.1mm) Relapse rate was 14.6% 2 Pog was moved backward (average 8.3mm) 48 hours following surgery The ratio of horizontal change of soft tissue to hard tissue at pog is 0.95 1 *In the cases of maxillary Le-Fort I osteotomy & mandibular sagittal split osteotomy. 3. A point was moved forward (average 3.31mm) 48 hours following surgery. 6 months later, it was returned backward (average 0.31) Relapse rate was 9 4% 4 6 months later, the ratio of facial convexity angle change of soft tissue to hard tissue is 0.63 1.

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Application of Constant Rate of Velocity or Pressure Change Method to Improve Annular Jet Pump Performance

  • Yang, Xuelong;Long, Xinping;Kang, Yong;Xiao, Longzhou
    • International Journal of Fluid Machinery and Systems
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    • v.6 no.3
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    • pp.137-143
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    • 2013
  • To improve annular jet pump (AJP) performance, new ways named constant rate of velocity/pressure change method (CRVC/CRPC) were adopted to design its diffuser. The design formulas were derived according to the assumption of linear velocity/pressure variation in the diffuser. Based on the two-dimensional numerical simulations, the effect of the diffuser profile and the included angle on the pump performance and the internal flow details has been analyzed. The predicted results of the RNG k-epsilon turbulence model show a better agreement with the experiment data than that of the standard and the realizable k-epsilon turbulence models. The AJP with the CRPC diffuser produces a linear pressure increase in the CRPC diffuser as expected. The AJP with CRPC/CRVC diffuser has better performance when the diffuser included angle is greater or the diffuser length is shorter. Therefore, the AJP with CRPC/CRVC diffuser is suitable for applications requiring space limitation and weight restriction.

Effects of Acceleration and Deceleration Parameters on the Machining Error for Large Area Laser Processing (대면적 레이저 가공을 위한 가감속 파라미터가 가공오차에 미치는 영향)

  • Lee, Jae Hoon;Yoon, Kwang Ho;Kim, Kyung Han
    • Journal of the Korean Society for Precision Engineering
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    • v.31 no.8
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    • pp.721-728
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    • 2014
  • In this paper, it is proposed a method of optimizing path parameters for large-area laser processing. On-the-fly system is necessary for large-area laser processing of uniform quality. It is developed a MOTF(Marking On-The-Fly) board for synchronizing the stage and scanner. And it is introduced the change of the error due to the change of parameters and algorithm for large-area laser processing. This algorithm automatically generates stage path and a velocity profile using acceleration and deceleration parameters. Since this method doesn't use a G-code, even if without expert knowledge, it has an advantage that can be accessed easily. Angle of one of the square of $350{\times}350mm$ was changed from $50^{\circ}$ to $80^{\circ}$ and analyzed the error corresponding to the value of Ta. It is calculated the value of Ta of the best with a precision of 20um through measurement of accuracy according to the Ta of each angle near the edge.

Effect of Vibrational Amplitude on Friction and Wear Properties of Magnetorheological Elastomer (진폭에 따른 자기유변탄성체의 마찰 특성 연구)

  • Lian, Chenglong;Lee, Kwang-Hee;Lee, Chul-Hee
    • Tribology and Lubricants
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    • v.32 no.2
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    • pp.39-43
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    • 2016
  • Magnetorheological elastomers (MREs) are a type of “smart” material, and their properties can be controlled rapidly and reversibly under the influence of an external stimulus. The application of an external magnetic field can change the shear modulus, hardness, and friction coefficient of MREs. The friction can cause vibration; moreover, the vibration can affect friction. The change of friction depends on the relative motion, normal force, roughness of the rubbing surfaces, material type, temperature, lubrication, relative humidity, and vibration condition. As MREs are a type of “smart material,” their friction coefficient can be reduced by applying an external magnetic field—the applications of this feature in engineering have been widely studied. However, the friction properties of MREs under vibration have not been tested to date. In this study, MRE samples and a reciprocating friction tester were fabricated. The friction coefficient was measured to evaluate the friction properties under various vibration conditions; subsequently, the wear depth and wear surface profile of the MRE were observed in order to evaluate the wear properties. The results show that the friction coefficient of the MREs decreased when a magnetic field was applied. Moreover, the friction coefficient decreased when the vibrational amplitudes increased. The wear depth of the MRE also decreased as the vibrational amplitudes increased.

Site-Directed Mutagenesis Studies with Restriction Endonuclease EcoRV to Identify the Role of Ile91 in Recognition and Catalysis

  • Moon, Byung-Jo;Vipond, I. Barry;Halford, Stephen E.
    • BMB Reports
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    • v.29 no.2
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    • pp.99-104
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    • 1996
  • Site-directed substitutions were made to change the Ile91 of restriction endonuclease EcoRV to either Val, Ala or Gly to identify the role of Ile91 in recognition and catalysis, since substitution of Ile91 with Leu afforded dramatic effects on the activity and properties of restriction endonuclease EcoRV. These changes alter the size of the hydrophobic side chain at position 91 and thus might have revealed the reason for the altered phenotype of Ile91Leu. However, the properties of Ile91Val and Ile91Ala mutants were much like wild type EcoRV, in both activity and metal ion preference. Ile91Gly had very little activity with either $Mg^{2+}$ or $Mn^{2+}$ as cofactors. To try to understand the unusual $Mn^{2+}$ profile of the Ile91Leu mutant, two double mutants, Ile91Leu;Asp90Asn and Ile91Leu;Glu45Met were created. Both double mutants were seriously disabled by the second amino acid change. Ile91Leu;Glu45Met had some residual activity in the $Mn^{2+}$ reaction buffer, whereas the Ile91Leu;Asp90Asn displayed no detectable activity.

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