• Journal of Microbiology and Biotechnology
• /
• v.5 no.3
• /
• pp.167-171
• /
• 1995

A bacterial strain C-02 using methanol as a carbon source was isolated from Gumi Industrial Estate and selected based on its rapid growth and capability of poly-$\beta$-hydroxybutyrate accumulation. Characteristics of strain C-02 showed that it belongs to the Methylococcaceae family, Type II subgroup. Strain C-02 could incorporate valerate into the PHB chain to form 3-hydroxybutyrate and 3-hydroxyvalerate (P(3HB-co-3HV)). Among various nutrient limitation tests, the nitrogen limitation test resulted in the highest content of P(3HB-co-3HV) per dry cell weight, 50$%$. Under the nitrogen limited condition, the average molecular weight of P(3HB-co-3HV) obtained was determined to be approximately $2.8\times 10^5$ daltons.

• Microbiology and Biotechnology Letters
• /
• v.24 no.4
• /
• pp.439-444
• /
• 1996

Microorganisms were collected to isolate the microbes producing Kochujang flavor from 3 kinds of traditional Kochujang. The strain B7 and Y20 which scored the highest sensory evaluation value among 400 microorganisms were finally selected. Also, they showed the most similar flavor pattern to the traditional Kochujang's by comparison of GC chromatogram after sniffing test. As the result of the result of the morphological and physiological experiments, the bacterial strain B7 could be identified Bacillus licheniformis and the yeast strain Y20 Saccaromyces dairensis. Especially, the bacterial strain B7 could grow in anaerobic condition and was able to hydrolysis the starch that was the major component in Kochujang.

• Natural Product Sciences
• /
• v.2 no.2
• /
• pp.90-95
• /
• 1996

When reserpine-producing cell strains of Rauwolfia serpentina were transferred from the dark to the light irradiation, the production of reserpine was extremely enhanced whereas the cell growth was suppressed. In an incubation period of 20 days, the most effective culture condition for reserpine production was the combination of 8 days of dark culture and following 12 days of light culture. The time courses of both cell growth and reserpine production were measured in vitro in order to clarify the effect of wave length range of light on the biosynthesis of reserpine. Although the growth of cultured cells which had been incubated under continuous red, yellow, and green lights, respectively, was similar to that of the cultured cells subcultured in the dark. The cells cultured under red light irradiation produced less reserpine than dark-grown cultures. Both blue and near-ultraviolet light inhibited the growth of cultured cells. The production of reserpine was strikingly enhanced by blue light, but was strongly inhibited by near-ultraviolet light.

• International Journal of Korean Welding Society
• /
• v.5 no.1
• /
• pp.29-34
• /
• 2005

Finite element analysis (FEA) and experimental studies on an elastic bending method have been performed in order to control the angular distortion at the fillet weldment for curved panel. Process parameters for the elastic bending method such as clamping span and release time were analyzed with reference to welding condition and geometric effect of the curved panel, which can minimize or prevent the angular distortion by producing a proper skin stress to the fillet weldment. The amounts of the angular distortion decrease almost in a linear manner with an increase in the skin stress. The skin stress required for non-angular distortion at the fillet weldment is strongly dependent on the plate thickness, not the heat intensity applied. The clamping span for obtaining uniform skin stress was defined as functions of the plate thickness and length of the free edge. Clamp should be removed after the fillet weldment is cooled down to room temperature for non-angular distortion. Effectiveness of the elastic bending method established was verified by its application to an actual curved panel.

• Korean Journal of Microbiology
• /
• v.20 no.3
• /
• pp.105-112
• /
• 1982

The strain of Aspergillus, 6368A, producing acid protease showing high activity was isolated from soil, as a result of wide research about mold group. This strain was identified as a species of Aspergillus tubingensis by the investigation of morphological characteristics. The change of the enzyme production under the various media and culture condition was also studied. The optimum pH and stability of crude acid protease are 2.5, 2.0~4.5 and the optimum temeprature and thermal inactivation waas shown $50^{\circ}C,\;55^{\circ}C$, respectively. From the result of the study on the effects of metal ions, it was found that $MnCl_2,\;CoCl_2,\;CuCl_2,\;SrCl_2,\;and\;NiCl_2$ slightly increased the enzyme activity, on the other hand $ZnCl_2,\;CaCl_2,\;MgCl_2,\;SLS,\;and\;KMnO_4$ decreased it.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.22 no.1
• /
• pp.40-48
• /
• 2010

The purpose of the research is to develop the high performance solar chemical reactor for producing hydrogen using steam reforming reaction of methane. A specific shape chemical reactor is suggested : spiral type reactor. The reactor is installed on the dish-type solar thermal system of Inha University. The temperatures, $CH_4$ conversion rates, and Hz proportion are measured. At specific condition, $CH_4$ conversion rates of the spiral type reactor are about 91%, and Hz proportion are about 66%. The spiral type reactor gives reasonably good performance without any problems caused by highly concentrated solar radiation.

• Korean Journal of Microbiology
• /
• v.12 no.1
• /
• pp.37-51
• /
• 1974

Three strains which accumulated large quantities of L-hlutamic acid as the chief product of metabolism in the presence of glucose and inorhanic ammonium salt under the aerobic condition sere newly isolated. These strains have the general characters as follows: Gram-positive, short fod-shaped, non-sporulating, non-motile, and facultative anaerobe. A change of cell shates was not almost observed in their life cycles and neither a phenomenon of cell-elongation nor a pleomorphism was recognized in any cases. Together with rigid stability in Gram-stain, these characters as above indicated evidences that newly isolates would belong to genus Brevibacterium clearly differentiated from genus Corynebacterium. At the same time the newly isolates, in addition to nutritional requirement of biotin and thiamine, showed the distinctive character of requirement of special amino acid such as histidine or cysteine for their considerable growth. These physiological characters including nitrates reduction, reaction on milk, and slow formation of acid from sugar also were useful in confirming that these bacteria would be Brevibacterium ammoniagenes.

• Proceedings of the Korea Concrete Institute Conference
• /
• 1995.04a
• /
• pp.183-188
• /
• 1995

Up to date, high-strength concrete pile which is producing in factory sells in the market. But according to the site and the construction conditions, the system to produce high-strength concrete pile directly in site is utilized in advanced country. Such the production system is the technique phenomenon very disirable in the side of quality control in site and the construction schedule, the time and the cost saving. This study is a fundamental experiment including concrete mixing design, non-autoclave curing method and the optimum condition to produce high-strengh concrete pile in site. As results of this study, High-strength concrete pile in site which target strength is 400kg/ $\textrm{cm}^2$ is able to produce it with optimum curing ciondition(75$^{\circ}C$, 9hr)and mixing design.

• 한국정보통신설비학회:학술대회논문집
• /
• 2007.08a
• /
• pp.151-154
• /
• 2007

This paper describes the project for constructing a traceability system for producing using RFID antenna in 900MHz frequency band and the consideration. In this project we reviewed analysis of properties classified by production process and RFID system through each frequency band, and designed a single unit RFID reader including antenna. The developed antenna is optimized to a field condition and this paper will show the test results. Also, we designed a single unit RFID reader by adding a reader to a designed antenna and explained the rule of tag memory allocation. Lastly, after running the system we could calculate visible and invisible effects of operation results and check up a wide possibility of application for RFID.

• 한국신재생에너지학회:학술대회논문집
• /
• 2009.06a
• /
• pp.787-790
• /
• 2009

There are several types of compressors which are appropriate for hydrogen gas station. Metal diaphragm type of hydrogen compressor is the one of them, a use in which satisfies the requirements of maintaining gas purity and producing high pressure over 700 bar. The objective of this study is to investigate an characteristics of compression as bulk modulus of oil varies. Three cases of bulk modulus ranging from $2{\times}10^9$, $4.52{\times}10^9$ and $7{\times}10^9$ were studied through FSI (Fluid Structure Interaction) analysis. Gas pressure, oil pressure and deflection degree of diaphragm were analysed during a certain period of compression process. Highest pressure and deflection were found in the condition of high bulk modulus of $7{\times}10^9$.