• 대한기계학회논문집B
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• 제21권1호
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• pp.140-152
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• 1997

A simultaneous measurement of the wall skin friction and near-wall streamwise velocity fluctuations is performed using hot film and hot wire anemometers to investigate the relation between them. Near-wall turbulence statistics measured with a hot-wire probe are in good agreement with previous results. Turbulence properties of the wall skin friction fluctuations measured with a hot film also show fairly good agreements with those measured by others except that rms level is lower in the present study. Long-time averaged space- time correlations show that the wall skin friction is highly correlated with a turbulence structure which is tilted from the wall in the streamwise direction. Tilting angles are obtained from the phase shifts between the wall skin-friction and streamwise velocity fluctuations. The convection velocity of the near-wall streamwise velocity obtained from the space-time correlation is in good agreement with that from the direct numerical simulation database.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.3101-3101
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• 2001

Near Infrared spectroscopy (NIR) used on human skin measurement was explored in the past decade. Many publications in different journals and magazines discussed the feasibility of the NIR technique for cosmetic product property studies. Based upon the results of pioneers, we have pursued some work of the NIR instrument coupled with a probe module for skin measurement in vivo and vitro. In part I of this paper, the specific Near Infrared spectroscopy instrument stability, human subject conditions and other parameters, which could affect the measurements reproducibility are discussed. Second derivative NIR spectra and Principle Components Analysis (PCA) are utilised for data interpretation. In part II of this paper, the relationship of human skin moisture and ageing, the gender information and finally, the discovery of penetration depth of NIR incident light on skin are reported. A theoretical penetration depth calculation equation is proposed. In part III, the study results of a couple of commercial skin care products effect will be described. The skin lotions were applied on human skin (in vivo) in order to exam the NIR feasibility to monitor the changes of moisture level. The results are consistently positive. From our primary study, it can conclude that the NIR is potentially a very powerful instrument for skin condition diagnostics, either for cosmetic and/or for medication purposes.

• 미생물학회지
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• 제30권3호
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• pp.232-238
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• 1992

Campylobacter jejuni 에 열처리를 했을 때 그들의 생존성 및 열충격 단백질 합성의 양상과 더불어, dnaK 와 groESL 유전자를 이용하여 C. jejuni 의 열충격 유전자를 검출하여 그 특성을 E. coli 의 열충격 유전자와 비교하였다. C. jejuni 의 열충격 단백질은 48.deg.C 에서 가장 잘 발형되었으며, 48.deg.C 에서 30 분간의 처리중 세포들의 생존율은 떨어지지 않았다. C. jejuni 의 열충격 단백질로서의 Hsp90, Hsp66, Hsp60 이 합성되는 것을 SDS-PAGE 및 방사선사진법을 통해 확인하였다. dnaK 와 groESL 을 DNA 탐침자로 이용하여 Southern hybridization 한 결과, C. jejuni 의 열충격 유전자도 groESL 과 dnaK 유전자와 상동성을 가진 염기서열을 가지고 있었으나, 두 균주사이에는 열충격유전자를 내포하고 있는 DNA 상에서 제한효소의 절단부위에 차이가 있었다.

• 대한의생명과학회지
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• 제22권4호
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• pp.115-126
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• 2016

Audiometric calibration is a prerequisite for securing the reliability of audiometric test results by checking the internal consistency of the relevant instrument. The purpose of this review is to help instrument operators understand the calibration procedure of aural acoustic immittance instrument which is frequently used for objective assessment. By referring to the latest international standards and the national standards relevant to the aural acoustic immittance instrument, the following parameters will be reviewed: 1) introduction of performance characteristics, 2) detailed procedure of the calibration method. According to the newest international and national standards [IEC 60645-5 (2004), ANSI S3.39-1987 (R2012)], the aural acoustic immittance instrument basically includes six components: 1) calibration cavity, 2) acoustic immittance analysis system, 3) probe assembly/unit and signal, 4) pneumatic air-pressure pump system, 5) acoustic reflex activator system and 6) tympanogram and acoustic reflex plotting system, each of these components should meet set standards. The result of behavioral hearing tests is influenced by various complex factors including the examinee's cooperation, background noise of the examination room, measurement method, skill level of the audiologist and calibration status, but the objective hearing tests is more influenced by the calibration status of the instrument than any other factors. The audiologist should take full responsibility for the reliability of the hearing test result, so he/she should carry out the calibration check and adjustments of aural acoustic immittance instrument periodically and maintain the instrument continuously by referring to the newest standards and the manufacturer's instruction manual.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2009년도 제33회 추계학술대회논문집
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• pp.395-398
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• 2009

Currently, a new generation of ducted fan UAVs (Unmanned Aerial Vehicles) is under development for a wide range of inspection, investigation and combat missions as well as for a variety of civil roles like traffic monitoring, meteorological studies, hazard mitigation etc. The current study presents extensive results obtained experimentally in order to investigate the tip clearance effects on performance characteristics of a ducted fan for small UAV systems. Three ducted fans having different tip clearance gap and with same rotor size were examined under three different yawed conditions of calibrated slanted hot-wire probe. Three dimensional velocity flow fields were measured from hub to tip at outlet of the ducted fan. The analysis of data were done by PLEAT (Phase locked Ensemble Averaging Technique) and three non-linear differential equations were solved simultaneously by using Newton -Rhapson numerical method. Flow field characteristics such as tip vortex and secondary flow were confirmed through axial, radial and tangential velocity contour plots. At the same time, the effects of tip clearance on axial thrust and input power were also investigated by using wind tunnel measurement system. For enhancing the performance of ducted fan, tip clearance level should be as small as possible.

• 한국과학교육학회지
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• 제23권4호
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• pp.401-418
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• 2003

The purpose of this study was to examine philosophical views on science of two influential curriculum documents, AAAS' s Benchmarks for Scientific Literacy (Benchmark) and NRC's National Science Education Standards (Standard), and to get educational implications about a desired philosophical view on science at a school science level. In order to determine the philosophical views on science explicitly suggested in the documents, Soh's Philosophical Perspectives Probe (PPP) was used as a framework for analysis. Forty preservice teachers reviewed the documents, extracting paragraphs with which statements of the PPP' s questions would agree. The results of the study were as follows: First, the Benchmark's philosophical view on science corresponds to the borderline between inductivism and eclecticism, or eclecticism close to falsificationism. The philosophical positions by the PPP' s themes are very different. Second, the Standard's philosophical position on science corresponds to inductivism close to eclecticism. Its philosophical position by the themes of the PPP is very different like the Benchmark. These results indicate that philosophical positions of the documents are more complex than popular conceptions would have it. That is to say, the results suggest that the science curriculum documents hold not only a contemporary philosophical view on science but also a traditional view on science, and that the philosophical positions on science are different from each other by documents and even by the PPP's themes in the same document. The results suggest that the philosophical views on science in school science contexts need to be adjusted and presented to K-12 students according to topics related to philosophy of science.

• 한국압력기기공학회 논문집
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• 제11권2호
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• pp.13-19
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• 2015

Steam generator is one of the most important component of nuclear power plant, and it's integrity and reliability are to be assured to high level by pre-service inspection and in-service inspection. To improve the reliability of steam generator heat exchanger tubes and to secure the management of nuclear power plant safely, KHNP CRI recently has developed eddy current testing system for steam generator. KHNP CRI have performed a series of experimental verification and field application to confirm the performance of the developed ECT system in accordance with ASME Code requirements. The ECT system consists of a remote data acquisition unit, an ECT signal acquisition and analysis software, a water chamber robot controller and a probe push-puller. In this paper, we will details of the developed ECT system and the software and their experimental performance. And also we will report the field applying performance and the issues for further steps.

• 대한치과보존학회:학술대회논문집
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• 대한치과보존학회 2003년도 제120회 추계학술대회 제 5차 한ㆍ일 치과보존학회 공동학술대회
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• pp.546.1-546
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• 2003

Laser doppler flowmeter (LDF) has been applied to the measurement of pulpal blood flow (PBF) in human teeth. As far as we searched, the detection area of the pulp in the blood flow measurement has not been clarified, yet. Therefore, the purpose of this study was to obtain information of the detection area in PBF measurement using LDF. The experiments were performed on the artificial blood circulation in extracted human upper central incisors. The apical portions of examined teeth (n=6) were severed and root canals were enlarged from the apical end to the 2mm incisal to the level of enamel-cement junction. An individual resin cap of each tooth was prepared and a hole was drilled 2mm incisal to enamel-cement junction of the labial side of the cap. The measurement probe of LDF (MBF3D, Moor Instrument, UK) was plugged into the hole of the cap. Heparinized human peripheral blood, which was in advance collected and diluted 3 times with physiological saline, was pumped through the apical foramen of the teeth via a silicone tube and a disposable needle (o.d. 0.7mm) and blood flow signals were monitored. The flux signal significantly increased with the enlargement of the root canal to incisal direction (p<0.01, Friedman analysis). The result indicates that the performance of LDF in PBF with human teeth is limited.

• Journal of Plant Biotechnology
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• 제42권3호
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• pp.186-195
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• 2015

Anthranilate synthase (AS)는 트립토판(Trp)과 indole-3-acetic acid, indole alkaloids의 생합성 경로에서 중요한 효소로 작용한다. 트립토판 생합성 상에서 feedback inhibition에 민감하게 반응하는 AS alpha-subunit 관련 OASA2 유전자 영역의 single (F124V) 및 double (S126F/L530D) 점돌연변이로 변형된 유전자의 재조합운반체를 작성하고 이들 유전자들을 Agrobacterium 방법으로 동진벼에 도입하여 형질전환체를 육성하였다. Single 및 double 돌연변이 OsASA2 유전자가 도입된 형질전환 벼 계통들은 nos gene probe를 이용한 TaqMan PCR 방법으로 single copy를 선발하였고, intergenic 계통을 선발하기 위해서 Bfa I 제한효소를 이용하여 RB와 LB 인접서열로부터 IPCR을 통한 FST 분석을 수행하여 4 개의 intergenic 계통을 선발하였다. 도입된 유전자의 발현으로 형질전환 벼는 Trp, IAN 및 IAA가 잎에 가장 많이 축적되었고, 종자의 트립토판 함량도 증가되었다. 후대에서 tryptophan 함량이 높은 S-TG와 D-TG의 두 호모 이벤트 계통을 육성하여 트립토판 함량을 분석한 결과 대조구에 비하여 13~30배 이상 높게 나타났으며, 유리아미노산의 함량도 증가하였다. 이벤트 계통을 이용하여 microarray 분석을 수행한 결과 세포 내 이온 수송, 영양분 공급 등에 영향을 주는 유전자군들이 up-regulation 되었고, 세포 내 기능유전자의 역할을 담당하는 조효소 등이 down-regulation 된 것을 확인 할 수 있었다. 이러한 결과는 선발된 두개의 상동성 이벤트 계통들이 고함량의 유리 트립토판 생산 벼의 육종에 효과적으로 이용될 수 있음을 보여준 결과로 생각된다.

• 식물조직배양학회지
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• 제25권5호
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• pp.341-346
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• 1998

색소유전자의 전이를 통하여 새로운 색소발현체계를 가진 품종을 육종하기 위한 기초연구로 stock (Matthiola incana R. Br.)의 꽃봉오리로부터 cDNA-library를 합성하였고 screening을 통하여 anthocyanin 합성경로의 중요효소의 하나인 DFR (dihydroflavonol 4-reductase) 유전자를 분리하였다. 염기서열분석을 수행하여 분리유전자의 크기가 1450bp 이며 이중 coding region은 1029 bp 임을 확인하였다. 이미 밝혀진 다른 식물체의 DFR 유전자와 서로 염기서열의 일치성을 비교해 본 결과 외자엽식물인 옥수수와 보리와는 각각 61%를 보였으며, 쌍자엽식물인 페튜니아, 금어초, 거베라, 과꽃 그리고 카네이션 등 과는 66%-67%의 일치성을 나타내었다. 아울러 염기서열의 G/C 함량분석을 통하여 쌍자엽식물의 G/C 함량은 외자엽식물의 그것에 비해 매우 낮은 수치를 나타내었다. 분리유전자의 발현을 확인하기 위하여 인위적으로 기내에서의 전사와 해석을 수행한 결과 42-44 kd 크기의 단백질을 확인하였다. Southern blot 분석의 결과 DFR 유전자는 stock의 genome에 다른 대부분의 식물체와 유사하게 한 개가 존재하며 야생종과 돌연변이종의 stock을 분리 DFR 유전자를 probe 로 Northern blot 분석을 수행하여 돌연변이종인 lineK17b가 DFR 돌연변이임을 확인하였다.