• Transactions of the Korean Society of Mechanical Engineers B
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• v.21 no.1
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• pp.140-152
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• 1997

A simultaneous measurement of the wall skin friction and near-wall streamwise velocity fluctuations is performed using hot film and hot wire anemometers to investigate the relation between them. Near-wall turbulence statistics measured with a hot-wire probe are in good agreement with previous results. Turbulence properties of the wall skin friction fluctuations measured with a hot film also show fairly good agreements with those measured by others except that rms level is lower in the present study. Long-time averaged space- time correlations show that the wall skin friction is highly correlated with a turbulence structure which is tilted from the wall in the streamwise direction. Tilting angles are obtained from the phase shifts between the wall skin-friction and streamwise velocity fluctuations. The convection velocity of the near-wall streamwise velocity obtained from the space-time correlation is in good agreement with that from the direct numerical simulation database.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.3101-3101
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• 2001

Near Infrared spectroscopy (NIR) used on human skin measurement was explored in the past decade. Many publications in different journals and magazines discussed the feasibility of the NIR technique for cosmetic product property studies. Based upon the results of pioneers, we have pursued some work of the NIR instrument coupled with a probe module for skin measurement in vivo and vitro. In part I of this paper, the specific Near Infrared spectroscopy instrument stability, human subject conditions and other parameters, which could affect the measurements reproducibility are discussed. Second derivative NIR spectra and Principle Components Analysis (PCA) are utilised for data interpretation. In part II of this paper, the relationship of human skin moisture and ageing, the gender information and finally, the discovery of penetration depth of NIR incident light on skin are reported. A theoretical penetration depth calculation equation is proposed. In part III, the study results of a couple of commercial skin care products effect will be described. The skin lotions were applied on human skin (in vivo) in order to exam the NIR feasibility to monitor the changes of moisture level. The results are consistently positive. From our primary study, it can conclude that the NIR is potentially a very powerful instrument for skin condition diagnostics, either for cosmetic and/or for medication purposes.

• Korean Journal of Microbiology
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• v.30 no.3
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• pp.232-238
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• 1992

Canz~~j~lohuc;tc.~jurn i werc studied for their heat shock responses at several elevated temperatures and their heat shock genes were detected by the technique of Southern hybridization. (.. ,jc\ulcorneruni sy~>thesized the major heat shock proteins of hsp90. hsphh. and hsphO at 48$^{\circ}$C . ant1 their w~u.ival rates were maintained as the same level at optimal temperature. '1-hc heat shock genes in chromosome of C ,jc:jutii werc determined to be homologous to the heat shock genes or E. t,oli. by showing strong signals in Southern hybridization analysis using clnaK and groESL- as DNA probe But the restriction sites for thc fragmcnts including heat shock genes were different betueen E. c,oli and C ,jtjuni.

• Biomedical Science Letters
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• v.22 no.4
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• pp.115-126
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• 2016

Audiometric calibration is a prerequisite for securing the reliability of audiometric test results by checking the internal consistency of the relevant instrument. The purpose of this review is to help instrument operators understand the calibration procedure of aural acoustic immittance instrument which is frequently used for objective assessment. By referring to the latest international standards and the national standards relevant to the aural acoustic immittance instrument, the following parameters will be reviewed: 1) introduction of performance characteristics, 2) detailed procedure of the calibration method. According to the newest international and national standards [IEC 60645-5 (2004), ANSI S3.39-1987 (R2012)], the aural acoustic immittance instrument basically includes six components: 1) calibration cavity, 2) acoustic immittance analysis system, 3) probe assembly/unit and signal, 4) pneumatic air-pressure pump system, 5) acoustic reflex activator system and 6) tympanogram and acoustic reflex plotting system, each of these components should meet set standards. The result of behavioral hearing tests is influenced by various complex factors including the examinee's cooperation, background noise of the examination room, measurement method, skill level of the audiologist and calibration status, but the objective hearing tests is more influenced by the calibration status of the instrument than any other factors. The audiologist should take full responsibility for the reliability of the hearing test result, so he/she should carry out the calibration check and adjustments of aural acoustic immittance instrument periodically and maintain the instrument continuously by referring to the newest standards and the manufacturer's instruction manual.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2009.11a
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• pp.395-398
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• 2009

Currently, a new generation of ducted fan UAVs (Unmanned Aerial Vehicles) is under development for a wide range of inspection, investigation and combat missions as well as for a variety of civil roles like traffic monitoring, meteorological studies, hazard mitigation etc. The current study presents extensive results obtained experimentally in order to investigate the tip clearance effects on performance characteristics of a ducted fan for small UAV systems. Three ducted fans having different tip clearance gap and with same rotor size were examined under three different yawed conditions of calibrated slanted hot-wire probe. Three dimensional velocity flow fields were measured from hub to tip at outlet of the ducted fan. The analysis of data were done by PLEAT (Phase locked Ensemble Averaging Technique) and three non-linear differential equations were solved simultaneously by using Newton -Rhapson numerical method. Flow field characteristics such as tip vortex and secondary flow were confirmed through axial, radial and tangential velocity contour plots. At the same time, the effects of tip clearance on axial thrust and input power were also investigated by using wind tunnel measurement system. For enhancing the performance of ducted fan, tip clearance level should be as small as possible.

• Journal of The Korean Association For Science Education
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• v.23 no.4
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• pp.401-418
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• 2003

The purpose of this study was to examine philosophical views on science of two influential curriculum documents, AAAS' s Benchmarks for Scientific Literacy (Benchmark) and NRC's National Science Education Standards (Standard), and to get educational implications about a desired philosophical view on science at a school science level. In order to determine the philosophical views on science explicitly suggested in the documents, Soh's Philosophical Perspectives Probe (PPP) was used as a framework for analysis. Forty preservice teachers reviewed the documents, extracting paragraphs with which statements of the PPP' s questions would agree. The results of the study were as follows: First, the Benchmark's philosophical view on science corresponds to the borderline between inductivism and eclecticism, or eclecticism close to falsificationism. The philosophical positions by the PPP' s themes are very different. Second, the Standard's philosophical position on science corresponds to inductivism close to eclecticism. Its philosophical position by the themes of the PPP is very different like the Benchmark. These results indicate that philosophical positions of the documents are more complex than popular conceptions would have it. That is to say, the results suggest that the science curriculum documents hold not only a contemporary philosophical view on science but also a traditional view on science, and that the philosophical positions on science are different from each other by documents and even by the PPP's themes in the same document. The results suggest that the philosophical views on science in school science contexts need to be adjusted and presented to K-12 students according to topics related to philosophy of science.

• Transactions of the Korean Society of Pressure Vessels and Piping
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• v.11 no.2
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• pp.13-19
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• 2015

Steam generator is one of the most important component of nuclear power plant, and it's integrity and reliability are to be assured to high level by pre-service inspection and in-service inspection. To improve the reliability of steam generator heat exchanger tubes and to secure the management of nuclear power plant safely, KHNP CRI recently has developed eddy current testing system for steam generator. KHNP CRI have performed a series of experimental verification and field application to confirm the performance of the developed ECT system in accordance with ASME Code requirements. The ECT system consists of a remote data acquisition unit, an ECT signal acquisition and analysis software, a water chamber robot controller and a probe push-puller. In this paper, we will details of the developed ECT system and the software and their experimental performance. And also we will report the field applying performance and the issues for further steps.

• Proceedings of the KACD Conference
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• 2003.11a
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• pp.546.1-546
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• 2003

Laser doppler flowmeter (LDF) has been applied to the measurement of pulpal blood flow (PBF) in human teeth. As far as we searched, the detection area of the pulp in the blood flow measurement has not been clarified, yet. Therefore, the purpose of this study was to obtain information of the detection area in PBF measurement using LDF. The experiments were performed on the artificial blood circulation in extracted human upper central incisors. The apical portions of examined teeth (n=6) were severed and root canals were enlarged from the apical end to the 2mm incisal to the level of enamel-cement junction. An individual resin cap of each tooth was prepared and a hole was drilled 2mm incisal to enamel-cement junction of the labial side of the cap. The measurement probe of LDF (MBF3D, Moor Instrument, UK) was plugged into the hole of the cap. Heparinized human peripheral blood, which was in advance collected and diluted 3 times with physiological saline, was pumped through the apical foramen of the teeth via a silicone tube and a disposable needle (o.d. 0.7mm) and blood flow signals were monitored. The flux signal significantly increased with the enlargement of the root canal to incisal direction (p<0.01, Friedman analysis). The result indicates that the performance of LDF in PBF with human teeth is limited.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.186-195
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• 2015

Anthranilate synthase (AS) is a key enzyme in the biosynthesis of tryptophan (Trp), which is the precursor of bioactive metabolites like indole-3-acetic acid and other indole alkaloids. Alpha anthranilate synthase 2 (OsASA2) plays a critical role in the feedback inhibition of tryptophan biosynthesis. In this study, two vectors with single (F124V) and double (S126F/L530D) point mutations of the OsASA2 gene for feedback-insensitive ${\alpha}$ subunit of rice anthranilate synthase were constructed and transformed into wildtype Dongjinbyeo by Agrobacterium-mediated transformation. Transgenic single and double mutant lines were selected as a single copy using TaqMan PCR utilized nos gene probe. To select intergenic lines, the flanking sequence of RB or LB was digested with a BfaI enzyme. Four intergenic lines were selected using a flanking sequence tagged (FST) analysis. Expression in rice (Oryza sativa L.) of the transgenes resulted in the accumulation of tryptophan (Trp), indole-3-acetonitrile (IAN), and indole-3-acetic acid (IAA) in leaves and tryptophan content as a free amino acid in seeds also increased up to 30 times relative to the wildtype. Two homozygous event lines, S-TG1 and D-TG1, were selected for characterization of agronomic traits and metabolite profiling of seeds. Differentially expressed genes (DEGs), related to ion transfer and nutrient supply, were upregulated and DEGs related to co-enzymes that work as functional genes were down regulated. These results suggest that two homozygous event lines may prove effective for the breeding of crops with an increased level of free tryptophan content.

• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.341-346
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• 1998

In this paper we describe the cloning and expression of the genes encoding the flavonoid-biosynthetic enzyme dihydroflavonol 4-reductase (DFR) in Matthiola incana R. Br. A heterologous cDNA probe from Zea mays was used to isolate full-size DFR cDNA clone from a corolla-specific cDNA library. Comparison of the coding region of this DFR cDNA sequence including the sequences of Zea mays, Anthirrinum majus, Petunia hybrida, Callistephus chinensis, Dianthus caryophyllus and Rosa hybrida reveals a identity higher than 61% at the nucleotide level. The DFR transcript is G/C rich in monocotyledonous plants show a strong codon bias preferring codons with a G or C in the third position. The function of this nucleotide sequences were verified by comparison with amino acid sequences of the amino-terminus and tryptic peptides from purified plant enzyme, by northern blotting with mRNA from wild type and mutant plants and by in vitro expression yielding an enzymatically active reductase. Genomic southern blot analysis showed the presence of one gene for DFR in Matthiola incana. Northern blot analysis of the DFR wild type and mutant lines showed that the lack of DFR activity in the stable acyanic mutant k17b is clearly by a transcriptional block of the DFR gene.