• Fisheries and Aquatic Sciences
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• 제24권4호
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• pp.163-170
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• 2021

Peptides are naturally occurring biological molecules that are found in all living organisms. These biologically active peptides play a key role in various biological processes. The aim of this study is the extraction and the purification of bioactive materials that induce relaxation of an apical muscle from the pyloric caeca of Patiria pectinifera. The acidified pyloric caeca extract was partially separated by the solid phase extraction using a stepwise gradient on Sep-Pak C18 cartridge. Among the fractions, materials eluted with 60% methanol/0.1% trifluoroacetic acid was put a thorough of a series of high performance liquid chromatography (HPLC) steps to isolate a neuropeptide with relaxation activity. The purified compound was eluted at 28% acetonitrile in 0.1% trifluoroacetic acid with retention time of 25.8 min on the CAPCELL-PAK C18 reversed-phase column. To determine the molecular weight and the amino acid sequence of the purified peptide, LC-MS and Edman degradation method were used, respectively. The primary structure of the peptide was determined to be FGMGGAYDPLSAGFTD which corresponded to the amino acid sequence of a starfish myorelaxant peptide (SMP) isotype (SMPb) found in the cDNA sequence encoding SMPa and its isotypes. In this study, a muscle relaxant neuropeptide (SMPb) has been isolated from pyloric caeca of starfish P. pectinifera. This is the first report of SMPb isolation on the protein level from P. pectinifera.

• 대한조선학회논문집
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• 제57권5호
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• pp.271-277
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• 2020

Recoverability and vulnerability of navy ships under underwater explosion are critical verification factors in the acquisition phase of navy ships. This paper aims to establish numerical analysis techniques for the underwater explosion of navy ships. Doubly Asymptotic Approach (DAA) Equation of Motion (EOM) of primary shock wave and secondary bubble pulse proposed by Geers-Hunter was introduced. Assuming a non-compressive fluid, reference solution of the DAA EOM of Geers-Hunter using Runge-Kutta method was derived for the secondary bubble pulse phase with an assumed charge conditions. Convergence analyses to determine fluid element size were performed, suggesting that the minimum fluid element size for underwater explosion analysis was 0.1 m. The spherical and cylindrical fluid domains were found to be appropriate for the underwater explosion analyses from the fluid domain shape study. Because the element size of 0.1 m was too small to be applied to the actual navy ships, a very slender beam with the square solid section was selected for the study of fluid domain existence effect. The two underwater explosion models with/without fluid domain provided very similar results in terms of the displacement and stress processes.

• Corrosion Science and Technology
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• 제21권3호
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• pp.221-229
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• 2022

To understand effects of cooling rates of coating layer on microstructures and corrosion behaviors of hot-dip alloy coated steel sheets (Zn-5%Al-2%Mg) in a neutral aqueous condition with chloride ion, a range of experimental and analytical methods were used in this study. Results showed that a faster cooling rate during solidification decreased the fraction of primary Zn, and increased the fraction of Zn-Al phase. In addition, interlamellar spacing became refined under a faster cooling rate. These modifications of the coating structure had higher open circuit potentials (OCP) with smaller anodic and cathodic current densities in the electrochemical potentiodynamic polarization. Surface analyses after a salt spray test showed that the increase in the Zn-Al phase in the coating formed under a faster cooling rate might have contributed to the formation of simonkolleite (Zn₅(OH)₈Cl₂·H₂O) and hydrotalcite (ZnAl₂(OH)₆Cl₂·H₂O) with a protective nature on the corroded outer surface, thus delaying the formation of red rust.

• Nuclear Engineering and Technology
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• 제54권9호
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• pp.3567-3579
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• 2022

This paper provides an assessment of fluid transport and mixing processes inside the primary circuit of the test facility ROCOM through the numerical simulation of Test 2.1 with the system code ATHLET. The experiment represents an asymmetric injection of cold and non-borated water into the reactor coolant system (RCS) of a pressurized water reactor (PWR) to restore core cooling, an emergency procedure which may subsequently trigger a core re-criticality. The injection takes place at low velocity under single-phase subcooled conditions and presents a major challenge for the simulation in lumped parameter codes, due to multidimensional effects in horizontal piping and vessel arising from density gradients and gravity forces. Aiming at further validating ATHLET 3-D capabilities against horizontal geometries, the experiment conditions are applied to a ROCOM model, which includes a newly developed horizontal pipe object to enhance code prediction inside coolant loops. The obtained results show code strong simulation capabilities to represent multidimensional flows. Enhanced prediction is observed at the vessel inlet compared to traditional 1-D approach, whereas mixing overprediction from the descending denser plume is observed at the upper-half downcomer region, which leads to eventual deviations at the core inlet.

• 전력전자학회논문지
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• 제27권5호
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• pp.384-390
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• 2022

In this study, a single-stage, four-phase, interleaved, totem-pole AC-DC converter is proposed for a super-fast charger station that requires high power, a wide voltage range, and bidirectional operation capabilities and adopts various types of electric transport vehicles. The proposed topology is based on current-fed push-pull dual active bridge converter combined with the totem-pole operation. Owing to the four-phase interleaving effect, the bridge on the grid side can switch at 0.25, 0.5, and 0.75 to achieve a ripple-free grid current. The input filter can be removed theoretically. Switching methods for the duty of the secondary-side duty cycle are proposed, and they correspond to the primary duty cycle for reducing the circulating power and handling the total harmonic distortion. Therefore, the converter can operate under a wide voltage range. Experimental results from a 7.5 kW prototype are used to validate the proposed concept.

• Journal of Positioning, Navigation, and Timing
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• 제10권4호
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• pp.307-313
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• 2021

Modernized GNSS signal structures tend to use tiered codes, and all GNSSs use binary codes as secondary codes. However, recently, signals using polyphase codes such as Zadoff-Chu sequence have been proposed, and are expected to be utilized in GNSS. For example, there is Tiered Differential Polyphase Code (TDPC) using polyphase code as secondary code. In TDPC, the phase of secondary code changes every one period of the primary code and a time-variant error is added to the carrier tracking error, so carrier tracking ambiguity exists until the secondary code phase is found. Since the carrier tracking ambiguity cannot be solved using the general GNSS receiver architecture, a new receiver architecture is required. Therefore, in this paper, we describe the carrier tracking ambiguity and its cause in signal tracking, and propose a receiver structure that can solve it. In order to prove the proposed receiver structure, we provide three signal tracking results. The first is the differential decoding result (secondary code sync) using the general GNSS receiver structure and the proposed receiver structure. The second is the IQ diagram before and after multiplying the secondary code demodulation when carrier tracking ambiguity is solved using the proposed receiver structure. The third is the carrier tracking result of the legacy GPS (L1 C/A) signal and the signal using TDPC.

• 한국건축시공학회지
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• 제23권4호
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• pp.497-508
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• 2023

Manual estimation of rebar quantities by contractors often yields discrepancies between projected and actual amounts used in the construction phase, leading to cost inaccuracies and potential logistical challenges. To address these issues, there is a clear need for a method that allows precise estimation of rebar quantities during the design phase. Such a method would enhance contractor competitiveness during project bids, promote accurate cost calculations, and avert superfluous rebar purchases on-site. Given that columns are the primary structural components in reinforced concrete(RC) buildings and necessitate considerable amounts of rebar, this study focuses on creating an automated algorithm for estimating column rebar quantities. An analysis of the accurate quantities obtained via the study and those derived from manual estimation reveals a discrepancy of 0.346 tons or 2.056%. This comparison affirms the proposed algorithm's efficacy in eliminating errors from overestimation or underestimation of rebar quantities. The practical implications of this study are significant for construction companies as it fosters efficient and precise estimation of rebar quantities, ensuring compliance with related specifications and governing regulations.

• 분석과학
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• 제36권5호
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• pp.205-215
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• 2023

In the present study, an analytical method was proposed for detecting trifluralin in aquatic products at trace concentrations. The method employed QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) and gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) as the sample preparation and measurement, respectively. The effect of the aqueous phase volume used in the QuEChERS was demonstrated, and the ratio of 10:10 (mL) between water and acetonitrile phase was used for 5 g of sample. Besides, dSPE using C18 and primary-secondary amine (PSA) was applied to remove the potential interferences from the food matrices, indicating no remarkable analyte loss. The linear range was built up from 0.50 ㎍ L^-1 to 3.0 ㎍ L^-1 (R² = 0.9993). Other criteria, i.e., repeatability (RSD_r = 0.86-1.96 %), reproducibility (RSD_R = 1.09-2.01 %), and recovery (over 90 %), were in accordance with Appendix F of AOAC (2016) for method performance. Although no trifluralin was detected for the commercial samples (fish, shrimp, and breaded shrimp), the spiked samples performed favorable recoveries and precision.

• 대한소아치과학회지
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• 제36권4호
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• pp.556-562
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• 2009

이소맹출은 유전적 요인 혹은 여러 가지 국소적 요인에 의해 치아가 비정상적인 위치로 맹출하는 경우를 말한다. 이소맹출이 가장 빈번히 발생하는 치아는 상악 제1대구치로 이를 방치할 경우 인접치의 치근흡수 및 이에 따른 조기탈락으로 영구치 맹출공간 부족을 야기하며, 결과적으로 부정교합을 유발하는 중요한 요인으로 작용한다. 그러나, 치료에 앞서 이소맹출중인 치아가 자발적으로 맹출하기를 기다려볼 것인지 혹은 조기에 치료를 하여 제2유구치의 치근흡수나 탈락을 예방해야 할 것인지에 대한 결정이 선행되어야 한다. 현재 이를 위한 몇몇 진단학적 기준이 제시되고 있으나 학자간 이견이 존재한다. 본 연구에서는 방사선학적 검사를 통해 상악 제1대구치의 이소맹출이 조기 발견된 증례들 중 전방에 위치한 제2유구치의 치근이 흡수되어 비가역적 특징을 보인다고 판단되는 증례에 대해 특별한 처치 없이 주기적인 검진을 통해 자발적으로 해소된 결과를 관찰하였다. 이와 함께 상악 제2유구치 치근흡수의 정도와 상악 제1대구치의 맹출 기울기에 따른 후향적 분석을 통해 상악 제1대구치 이소맹출의 유형을 예측하고자 하였다. 상악 제2유구치의 치근 흡수 정도에 따라 grade I에서 IV로 분류하였고, 파노라마 방사선 사진 상에서 양측 안와의 최하방점을 연결한 수평선과 상악 제1대구치의 근심면을 따라 연결한 수직선을 기준으로 하여 근심각(mesial angle)을 측정하였다. 결과적으로는 상악 제2유구치의 치근 흡수 정도보다 상악 제1대구치의 맹출 기울기가 이소맹출의 유형을 진단하는 데에 있어 더 신뢰할만한 기준이라고 판단할 수 있었다.

• 대한약리학회지
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• 제32권1호
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• pp.13-21
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• 1996

세포내 칼슘농도는 신경세포의 다양한 기능에 매우 중요한 역할을 하고 있다. 본 연구에서는 일차배양한 마우스 소뇌과립세포에서 니코틴성 아세틸콜린 수용체가 특정 발생단계에 발현되고 세포내 칼슘의 농도조절에 관여하는 것을 관찰하였다. 니코틴에 의한 세포내 칼슘농도의 변화는 $^{45}Ca^{2+}$나 fura-2를 사용하여 형광법으로 측정하였다. 니코틴은 마우스 소뇌과립세포내 칼슘의 농도를 최대한 증가시키는 것으로 보인다. 반면에 일차배양한 Glia 세포들에서는 $^{45}Ca^{2+}$ 농도를 증가시키지 않았다. 세포내 칼슘농도에 미치는 니코틴의 효과는 NMDA 수용체에 대한 길항제에 의하여 억제되었다. 또한 Glutamate pyruvate transminase (GPT)를 사용하여 배양액의 글루타민산을 제거하면 니코틴효과가 소실되는 것이 관찰되었다. 이러한 결과는 니코틴에 의한 세포내 칼슘농도의 변화가 세포에서 유리된 글루타민산에 의한 간접적인 효과임을 암시한다. Fura-2를 사용한 형광법으로 실험한 결과 니코틴은 two phase로 세포내 칼슘농도를 증가시키는 것을 보여주었다. NMDA 수용체 길항제와 GPT는 단지 후기 plateau상만 억제하였다. 따라서 본 연구결과는 니코틴이 직접 니코틴성 아세틸콜린 수용체를 자극하여 일시적으로 세포내 칼슘농도를 증가시키고 글루타민산을 유리하여 NMDA 수용체를 활성화시킴으로써 세포내 칼슘농도를 지속적으로 증가시키는 것으로 보여진다. 이러한 결과는 니코틴성 아세틸콜린 수용체가 특정한 발생과정에 발현되어 세포내 칼슘농도 조절에 관여함으로써 신경발생과정에서 중요한 역할을 할 수 있음을 보여주고 있다. state를 나타내는 것을 알 수 있다. 또한 $[^3H]DPCPX$를 이용한 competitive binding assay에서 0.1 mM GTP는 효현제인 PIA의 apparent affinity를 감소시켰으며, DPCPX의 apparent affinity는 증가시키고, CGS-15943에는 아무런 영향을 미치지 않았다. 이것은 상기의 $[^{35}S]GTP_{\gamma}S$ binding의 결과를 뒤받침해 주는 결과라고 생각된다.요한 역할을 할 수 있으리라 사료된다.X>$Ca^{2+}$에 의하여 활성화되는 $K^+$ 통로를 개방시킴으로 세포내 $Ca^{2+}$을 감소시켜 뇌 기저동맥의 이완반응을 매개하는 것으로 사료된다. 함량을 조정하므로, 흉선세포의 apoptosis에 억제적으로 작용할 수 있음을 시사하는 것으로 사료된다. 영양액에 의하여는 회복됨을 볼 수 있었으며 $Mg^{++}$ 증가 영양액에서는 억제, TTX 동시 투여시에는 완전히 소실되었다. 이상의 실험결과로 흰쥐 해마에서 $A_1-adenosine$ 수용체를 통한 adenosine의 NE 유리 감소는 TEA 및 4AP에 예민한 $K^+$-통로가 관여하고 여기에는 세포외액의 Ca^{++}의 농도가 중요한 인자의 하나로 관여 하는 것으로 사료된다. 영상의 질을 크게 향상 시켜 줌으로 비가역 3구획모델에서의 PGA방법을 대체할 새로운 파라메터 영상구성방법으로 적합할 것이다.관계되며, YH439는 중금속으로 유도된 조직독성에 방어효과가 있음을 지지한다.총 아미노산의 순은