• 한국식품저장유통학회지
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• 제5권3호
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• pp.305-314
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• 1998

In the drying process, many undesirable physicochemical changes occur that influence dried food product qualities. Pretreatments method is used to reduce the deterioration of dried food product qualities such as color, flavor, texture, rehydration ability and retention of nutrients. The methods of pretreatments are blanching, chemical treatment and osmotic dehydration. Osmotic dehydration is a water removal process which is based on placing foods in a concentrated osmotic solution or in a dry osmotic material. A large number of process variables have a significant effect on process and final product quality. In order to improve final product quality it is necessary to know the role of each process variable and understand the mecanisms throughout the process. Osmotic dehydration is a valuable processing tool with great future in minimal processing of fruits and vegetables.

• 한국식품영양과학회지
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• 제26권5호
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• pp.807-813
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• 1997

Various additives(glucono-$\delta$-lactone, glycine, chitosan, Chinese pepper extract+msutard oil, cinnamon oil+ginger oil+mustard oil, Chinese pepper extract), used in the Korean patents singularly or in combination, were tested for extension of shelf-life of kimchi. Addition of glucono-$\delta$-lactone or chitosan was somewhat effective in delaying the fermentation rate, however no such effect was seen by other additives. Chitosan at the concentrations of 0.5, 1.0, and 1.5% was similarily effective in delaying the fermen-tation rate. Mustard oil or cinnamon oil tend to delay the fermentation rate by singular addition at the concern tration of 0.5% or 1%. Soaking of the salted and washed Chinese cabbage in 0.5% chitosan solution resulted in delay of the fermentation rate of kimchi.

• Journal of Chest Surgery
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• 제21권2호
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• pp.231-239
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• 1988

Currently numerous methods are in use for myocardial hypothermia as a myocardial preservation modality for cardiac operation. During cardiac ischemia after crystalloid cardioplegia[4C GIK solution], topical cold saline[Group I, a=9], topical ice slush[Group II, n=9] and topical ice chip[Group III, a=10] have been compared for myocardial surface cooling in the isolated rat heart model of cardiopulmonary bypass. During postischemic period, hemodynamic functions[aortic flow, coronary flow, peak aortic pressure and heart rate], biochemical enzymatic activities and cellular injuries with electron microscope were evaluated in this isolated rat heart perfusion model. Postischemic aortic flow, cardiac output and peak aortic pressure in Group I and Group II recovered better than Group III.[p< 0.05] Postischemic creatine kinase and lactate dehydrogenase leakages in Group II and Group III increased more than Group l and postischemic mitochondrial swelling in Group III was more severe than Group I, and Group II.[p< 0.05] These results suggest that topical cold saline was the better method than topical ice slush or topical ice chip as a myocardial preservation modality in the isolated rat heart model of cardiopulmonary bypass.

• KIEAE Journal
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• 제2권3호
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• pp.47-54
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• 2002

As the problems of environment have been increasing, the architecture is exploring ways toward development of a idea for future generation. The purpose of this study is design element of environment-friendly architecture, and a general concept and mark of environment-friendly architecture are considered. And then, the design element is analyzed to come true the mark by considering the internal and external examples. The related elements on design of environment-friendly architecture are proposed by considering estimation items: The items are divided into six branches that is placement strategy, outside space furtherance, form of building, room environment furtherance, supply processing system, and preservation administration. Through this process, this study is proposed applicable solution of a concrete plan.

• 원예과학기술지
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• 제30권1호
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• pp.79-84
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• 2012

An efficient protocol for cryopreservation of madder hairy root cultures has been developed using droplet-vitrification. In previous study, combining loading solution C4 (35% PVS3) and vitrification solution B5 (80% PVS3) was the most effective method. In this study, we tried three types of vitrification solution, B5, A3 (90% PVS2, on ice), and A5 (70% PVS2, on ice). Combining loading solution C4 and vitrification solution A5 (on ice) showed the best regeneration rate in this study. Histological changes of the cells within the hairy root of madder were also observed in different steps. The cells from the hairy roots of the control treatment were full and intact with different size of vacuoles and obvious cell nucleus having a dark nucleolus. After the stage of preparing for cryopreservation (after preculturing, loading, followed by dehydration solution A5 or B5), intercellular spaces had become distinct, and within cells, the cytoplasms had become denser and week plasmolyses had appeared. The cell plasmolyses were much more apparent and we measured the degree of plasmolysis by calculating, the area of cell/the area of cytoplasm. The value of plasmolysis degree was the highest in the combination of preculture, loading solution C4, and dehydration solution A5, 1.97. Because the highest regeneration rates appeared in the treatment of A5 for 20 min, we could assume that the optimal degree of plasmolysis for cryopreservation might be around 1.97. The changes in cell structure during cryopreservation might be a useful basis for the development of a proper long-term preservation method for madder germplasms.

• 한국식품저장유통학회지
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• 제12권6호
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• pp.534-539
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• 2005

항균효과가 있는 자몽종자추출물과 은이온 용액을 이용하여 숙주나물의 유통 중 신선도 연장을 위하여 $30\;{\mu}m$ polypropylene(PP)필름에 숙주나물 200 g을 침지하지 않은 처리를 대조구로 하여 은이온 용액, GFSE 50 ppm 및 100 ppm 처리, 은이온 용액에 GFSE 50 ppm 및 100 ppm 처리를 하여 밀봉 포장한 후 냉장 온도인 $5^{\circ}C$에 저장하면서 실험한 결과이다. 저장 6일째 포장내 이산화탄소와 산소농도를 보면, 이산화탄소농도는 $4.2\~5.3\%$의 농도를 나타내었고, 산소농도는 $1.0\~1.1\%$농도를 나타내었다. 중량감소율은 모든 처리에서 $1.0\%$ 내외의 감소율을 보여, 중량 감소율에서는 큰 차이를 보이지 않았다. 경도는 모든 처리에서 저장 4일 이후에 낮아지는 경향이었고, 색도변화는 자엽과 배축 부분의 경우 저장4일째까지 모두 큰 차이를 보이지 않았고, 저장 6일째의 경우 자엽부분에서 갈변이 심하였다. 저장 중 숙주나물의 비타민C함량의 경우 침지를 하지 않은 처리(대조구)에서 비타민C 함량이 가장 낮은 반면, 다른 모든 처리에서 저장 기간동안 비타민C함량이 서서히 낮아져서 지연되는 효과를 나타내었다. 미생물변화에서는 총균수의 경우 대조구에 비해 자몽종자추출물과 은이온 용액에서 미생물의 증식이 억제됨을 알 수 있었고, 황색포도상구균의 경우 은이온 용액과 은이온 용액에 GFSE 100 ppm을 첨가한 처리에서는 저장 기간동안 전혀 검출이 되지 않았으며, 효모수는 저장기간에 따라 점차 증가하는 대조구와 달리 자몽종자추출물과 은이온 용액에서 효모의 증식이 억제됨을 알 수 있었다. 따라서 대조구의 상품성 유지기간이 2일인데 반해, GFSE 100 ppm처리와 은이온 용액에 GFSE 100 ppm 농도로 처리한 것이 6일로 4일정도의 신선도 연장과 함께 상품성이 있으므로 유통 시 미생물을 억제시킬 수 있고, 항균효과가 있는 세척수를 이용하는 것도 저장기간 연장과 함께 신선도 유지에 있어서 도움이 될 수 있다.

• Journal of Chest Surgery
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• 제12권4호
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• pp.383-394
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• 1979

Treatment of valvular heart disease with valve replacement has been one of the most popular procedures in cardiac surgery recently. Although, first effort was directed toward the prosthetic valve, it soon became popular that bioprosthesis, the valvular xenograft, was prefered in the majority cases. Valvular xenograft has some superiority to the artificial prosthetic valve in some points of thromboembolism and hemolytic anemia, and it also has some inferiority of durability, immunologic reaction and resistance to Infection. Tremendous efforts were made to cover the inferiority with several methods of collection, preservation, and valve mounting of the porcine valve or pericardium of the calf, and also with surgical technique of the valvular xenograft replacement. Auther has collected 320 porcine aortic valves immediately after slaughter, and aortic cusps were coapted with cotton balls in the Valsalva sinuses to protect valve deformity after immersion in the Hanks' solution, and oxidation, cross-linking and reduction procedures were completed after the proposal of Carpentier in 1972. Well preserved aortic valves were suture mounted in the hand-made tissue valve frame of 19, 21, and 23 mm J.d., and also in the prosthetic vascular segment of 19 mm Ld. with 4-0 nylon sutures after careful trimming of the aortic valves. Completed valves were evaluated with bacteriologic culture, pressure tolerance test with tolerane gauge, valve durability test in the saline glycerine mixed solution with tolerance test machine in the speed of 300 rpm, and again with pathologic changes to obtain following results: 1. Bacteriologic culture of the valve tissue in five different preservation method for two weeks revealed excellent and satisfactory result in view of sterilization including 0.65% glutaraldehyde preservation group for one week bacteriologic culture except one tissue with Citobacter freundii in 75% ethanol preserved group. 2. Pressure tolerance test was done with an apparatus composed of V-connected manometer and pressure applicator. Tolerable limit of pressure was recorded when central leaking jet of saline was observed. Average pressure tolerated in each group was 168 mmHg in glutaraldehyde, 128 mmHg in formaldehyde, 92 mmHg in Dakin's solution, 48 mmHg in ethylene oxide gas, and 26 mmHg in ethanol preserved group in relation to the control group of Ringer's 90 mmHg respectively. 3. Prolonged durability test was performed in the group of frame mounted xenograft tissue valve with 300 up-and-down motion tolerance test machine/min. There were no specific valve deformity or wearing in both 19, 21, and 23 mm valves at the end of 3 months (actually 15 months), and another 3 months durability test revealed minimal valve leakage during pressure tolerance test due to contraction deformity of the non-coronary cusp at the end of 6 months (actually 30 months) in the largest 23 mm group. 4. Histopathologic observation was focussed in three view points, endothelial cell lining, collagen and elastic fiber destructions in each preservation methods and long durable valvular tolerance test group. Endothel ial cell lining and collagen fiber were well preserved in the glutaraldehyde and formaldehyde treated group with minimal destruction of elastic fiber. In long durable tolerance test group revealed complete destruction of the endothelial cell lining with minimal destruction of the collagen and elastic fiber in 3 month and 6 month group in relation to the time and severity. In conclusion, porcine xenograft treated after the proposal of Carpentier in 1972 and preserved in the glutaraldehyde solution was the best method of collection, preservation and valve mounting. Pressure tolerance and valve motion tolerance test, also, revealed most satisfactory results in the glutaraldehyde preserved group.

• 정보교육학회논문지
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• 제7권1호
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• pp.27-36
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• 2003

환경문제의 해결을 위해서는 공학, 행정, 법률 등 여러 분야에서의 노력이 필요하지만 가장 근본적인 해결책은 국민 각자가 환경 보전에 대한 실천의지를 갖도록 하는 교육에서 찾아야 한다. 특히 유년기 즉 초등학생 시절부터 환경교육을 통하여 환경 보전 습관을 형성하는 것이 바로 환경을 지키는 최선의 방법이다. 본 논문에서는 초등학교 제7차 교육과정에 포함된 환경 관련 내용을 추출하여 글쓰기, 그리기, 자연의 소리듣기, 음악 듣기, 보고서 작성, 퀴즈 풀기 등의 다양한 활동을 통하여 환경에 관련된 지식, 기능을 습득하고 환경을 지키는 생활 습관과 태도를 형성하도록 하는 시스템을 설계하였다. 특히 최신의 적절한 학습자료를 제공하고 학생들의 창의성과 자율성이 충분히 발휘될 수 있도록 하기 위해 웹 기반 시스템으로 설계하였고, 구성주의 학습원리를 기반으로 학습자가 자기주도적으로 활동함으로써 환경을 지키려는 실천의지를 기르도록 하는 학습 모형을 설계하고 적용하였다. 또한 학습실, 환경 자료실, 환경 퀴즈실, 작품 전시실 등을 통하여 다양한 학습 활동과 동기유발을 지원할 수 있도록 하였고 교사는 학생들의 학습 상황을 쉽게 파악하고 평가할 수 있도록 구현하였다. 따라서 본 시스템은 학생들에게 환경과 관련된 지식, 기능, 태도, 참여 등의 환경교육의 목표를 달성하는데 있어서 그 효과가 기대된다.

• Journal of Veterinary Science
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• 제25권2호
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• pp.29.1-29.11
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• 2024

Background: Preservation of biological tissues has been used since ancient times. Regardless of the method employed, tissue preservation is thought to be a vital step in veterinary surgery teaching and learning. Objectives: This study was designed to determine the usability of chemically preserved cadaveric equine heads for surgical teaching in veterinary medicine. Methods: Six cadaveric equine heads were collected immediately after death or euthanasia and frozen until fixation. Fixation was achieved by using a hypertonic solution consisting of sodium chloride, sodium nitrite and sodium nitrate, and an alcoholic solution containing ethanol and glycerin. Chemically preserved specimens were stored at low temperatures (2℃ to 6℃) in a conventional refrigerator. The specimens were submitted to gross and organoleptic assessment right after fixative solution injection (D0) and within 10, 20, and 30 days of fixation (D10, D20, and D30, respectively). Samples of tissue from skin, tongue, oral vestibule, and masseter muscle were collected for histological evaluation at the same time points. Results: Physical and organoleptic assessments revealed excellent specimen quality (mean scores higher than 4 on a 5-point scale) in most cases. In some specimens, lower scores (3) were assigned to the range of mouth opening, particularly on D0 and D10. A reduced the range of mouth opening may be a limiting factor in teaching activities involving structures located in the oral cavity. Conclusions: The excellent physical, histologic, and organoleptic characteristics of the specimens in this sample support their usability in teaching within the time frame considered. Appropriate physical and organoleptic characteristics (color, texture, odor, and flexibility) of the specimens in this study support the use of the method described for preparation of reusable anatomical specimens.

• Journal of Chest Surgery
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• 제33권8호
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• pp.605-612
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• 2000

Background: Ischemic preconditioning enhances the tolerance of myocardium against ischemia/reperfusion injury, with the enhancement of the recovery of post-ischemic myocardial function. This study was disigned to assess whether the protective effect of ischemic preconditioning could provide one additional hour of myocardial preservation in four hour myocardial ischemia in a rate heart. Material and method: Fourty four Spargue-Dawley rats, weighing 300~450gm, were divided into four groups. Group 1(n=7) and group 3(n=12) were subjected to 30 minutes of aerobic Langendorff perfusion without ischemic preconditioning and then preserved in saline solution at 2~4$^{\circ}C$ for 4 hours and 5 respectively. Group 2(n=7) and group 4(n=18) were perfused in the same way for 20 minutes, followed by 3 minutes of global mormothermic ischemia and 10 minutes of perfusion and then preserved in the same cold saline solution for 4 hours and 5 hours respectively. Heart rate, left ventricular developed pressure(LVDP), and coronary flow were measured at 15 minutes during perfusion as baseline. Spontaneous defibrillation time was measured after reperfusion. Heart rate, LVDP, and coronary flow were also recorded at 15 minutes, 30 minutes, and 45 minutes during reperfusion. Samples of the apical left ventricular wall were studied using a transmission electron microscope. Result: Time of spontaneous defibrillation(TSD) was significantly longer in group 4 than in group 1(p<0.001), and TSD in group 1 was significantly longer in comparision to that of group 2(p<0.05). Heart rate at 45 minutes was significantly higher in group 1 than in group 4(p<0.05). Heart rate at 15 min was significantly higher in group 2 than in group 1(p<0.001) and in group 4 than in group 3(p<0.05). Left ventricular developed pressure(LVDP) at 30 minutes and 45 minutes was higher in group 1 than in group 4(p<0.01), LVDP at 45 minutes was higher in group 4 than in group 3(p<0.05). Rate-pressure product(RPP) at 30 minutes and 45 minutes was higher in group 1 than in group 4(p<0.05). RPP at 15 minutes was higher in group 2 than in group 1(p<0.01). RPP at 30 minutes and 45 minutes was higher in group 4 than in group 3(p<0.05). Group 2 showed relatively less sarcoplasmic edema and less nuclear chromatin clearance than group 1. Group 4 showed less myocardial cell damage than group 3, group 4 showed less myocardial cell damage than group 3, group 4 showed more myocardial cell edema than group 1. Conclusion: Ischemic preconditioning enhanced the recovery of postischemic myocardial function after 4 hours and 5 hours preservation. However, it was not demonstrated that ischemic preconditioning could definitely provide one additional hour of myocardial preservation in four hour myocardial ischemia in a rat heart.