• Title/Summary/Keyword: preparation technique

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Study on the Establishing a Guideline for Selecting Major Environmental Factors by the Project Type Criteria in EIA (환경영향평가 대상사업별 중점평가항목의 도출에 관한 연구)

  • Sung, Hyun-Chan;Han, Sang-Wook
    • Journal of Environmental Impact Assessment
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    • v.3 no.1
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    • pp.9-30
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    • 1994
  • Environmental Impact Assessment System, ever since its enactment in Korea fourteen years ago, has been steadily improved by introduction and implementation of new concepts and methodologies which include Environmental Impact Statement(EIS) preparation technique, selection of environmental factors, and the like. However, current guideline requires that every EIS, regardless of the specifics of the project type or characteristics of the site, must evaluate and study for all twenty-two environmental factors as set forth in the regulation. The situation causes many undesirable problems like; lack of site specific evaluation in most EISs, over-sized EIS drafts, consuming too much time and costs for EIS preparation, and so on. The objectives of this study are: - To establish a set of criteria to classify the types of the projects in terms of features of the project content and the characteristics of the site area. - To establish a guideline for selecting major environmental factors of the project type criteria, and - To provide basic materials for the preparation of complets draft EIS of the Amendment of Environmental Impact Assessment Act.

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Effect of Caerulein on the Isolated Gall Bladder Strips of Rabbits (토끼 담낭절펼에 대한 Caerulein의 작용)

  • Cho, T.S.;Lee, W.C.;Hong, S.S.
    • The Korean Journal of Pharmacology
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    • v.10 no.1 s.15
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    • pp.47-52
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    • 1974
  • The isolated rabbit gall bladder strips were prepared according to the technique described by Amer and Becvar (1969). The strips were placed in a bath containing 100 ml of Locke-Ringer solution maintained at $38^{\circ}C$. Oxygen was continuously bubbled through the solution. The tension of the muscle strip was initially adjusted to 0.7g. The contractile response was measured isometrically by a force-displacement transducer connected to a polygraph. The effect of a number of autonomic drugs were studied for their interaction with caerulein (Prof. V. Erspamer, F.I. 6934 Caerulein, Farmitalia, Italia), a gastrin or CCK.PZ like peptide, on isolated rabbit gall bladder strips. In this preparation caerulein produced contractions of CCK-PZ type, but the relative potency on a weight basis was 40 times that of CCK-PZ. The response of caerulein was not modified by either cholinergic or alpha or beta adrenergic blockade. However, the response of caerulein and of barium on the strips were prevented by papaverine or aminophylline. Isoproterenol, papaverine or aminophylline alone relaxed the preparation whereas caerulein, CCK-PZ, acetylcholine, serotonin, histamine or barium chloride contracted the preparation. In summary, it is concluded that caerulein on the gall bladder strip seems to act independently of the autonomic nervous system and mediated via mechanisms apparently similar to those involved in the action of barium chloride.

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Evaluation of Amplified-based Target Preparation Strategies for Toxicogenomics Study : cDNA versus cRNA

  • Nam, Suk-Woo;Lee, Jung-Young
    • Molecular & Cellular Toxicology
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    • v.1 no.2
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    • pp.92-98
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    • 2005
  • DNA microarray analysis of gene expression in toxicogenomics typically requires relatively large amounts of total RNA. This limits the use of DNA microarray when the sample available is small. To confront this limitation, different methods of linear RNA amplification that generate antisense RNA (aRNA) have been optimized for microarray use. The target preparation strategy using amplified RNA in DNA microarray protocol can be divided into direct-incorporation labeling which resulted in cDNA targets (Cy-dye labeled cDNA from aRNA) and indirect-labeling which resulted in cRNA targets (i.e. Cy-dye labeled aRNA), respectively. However, despite the common use of amplified targets (cDNA or cRNA) from aRNAs, no systemic assessment for the use of amplified targets and bias in terms of hybridization performance has been reported. In this investigation, we have compared the hybridization performance of cRNA targets with cDNA targets from aRNA on a 10 K cDNA microarrays. Under optimized hybridization conditions, we found that 43% of outliers from cDNA technique and 86% from the outlier genes were reproducibly detected by both targets hybridization onto cDNA microarray. This suggests that the cRNA labeling method may have a reduced capacity for detecting the differential gene expression when compared to the cDNA target preparation. However, further validation of this discordant result should be pursued to determine which techniques possesses better accuracy in identifying truly differential genes.

A Study on the Optimization plan for Standardization of Engineering Drawing (설계도면 작성기준 최적화 방안에 관한 연구)

  • Kim, Suk-Su;Song, Jae-ho
    • Journal of the Korean Society of Industry Convergence
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    • v.23 no.1
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    • pp.57-64
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    • 2020
  • The construction market became generally opened to overseas and the domestic construction industry has competed with the international construction enterprise of which technical knowledge is superior. In order to compete with the international construction companies, domestic construction industry needs more continuous promotions on the research and development for improving the level of technique of the construction engineering to meet the international norm. This study aims to suggest the better standards of engineering output such as drawings and specifications to correspond the changing integration system and bidding system in preparation for growing international competition. The study starts with comparison and analyses of domestic and international methods and standards for preparing design drawings. And it continues to delve into actual drawing preparation practices, especially focusing on the ones for road and subway construction. Finally, the study presents a better way of the standardization of the main drawings such as general plan, longitudinal drawing, cross section, structural drawings and temporary structure for new bidding systems of historical cost estimation and whole specification tender and for promotion of international competitiveness.

ABILITY OF NICKEL-TITANIUM FILES IN MAINTAINING THE ORIGINAL CURVATURE OF A CURVED ROOT CANAL AND ITS ANALYSIS BY FINITE ELEMENT METHODS (만곡 근관 형성시 니켈-티타늄 파일의 만곡 유지 능력과 그에 대한 유한요소법적 분석)

  • Park, Han-Soo;Lim, Sung-Sam;Bae, Kwang-Shik;Yoon, Soo-Han
    • Restorative Dentistry and Endodontics
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    • v.22 no.1
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    • pp.220-227
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    • 1997
  • The purpose of our study is to evaluate the ability of nickel-titanium(NiTi) files in maintaining the original curvature of a curved root canal during canal preparation. Curved canals on translucent resin blocks were prepared with NiTi and stainless steel files, and they were placed at the platform which can reproduce the same position. The unprepared and prepared canal forms were accurately compared by double exposure technique of photography. By finite element methods we also analyzed stress distributions of NiTi and stainless steel files in a curved canal. The results were as follows : 1. NiTi files were excellent in maintaining the original curvature of a curved canal than stainless steel files after canal preparation. 2. The results of canal preparation with these files were well verified by the analysis of stress distributions using finite element methods.

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Superficial and Deep Skin Preparation with Povidone-Iodine for Ventriculoperitoneal Shunt Surgery : A Technical Note

  • Craven, Claudia L.;Thompson, Simon D.;Toma, Ahmed K.;Watkins, Laurence D.
    • Journal of Korean Neurosurgical Society
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    • v.62 no.1
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    • pp.123-129
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    • 2019
  • Objective : Ventriculoperitoneal (VP) shunt surgery is a common and effective treatment for hydrocephalus and cerebrospinal fluid disorders. Infection remains a major cause of morbidity and mortality after a VP shunt. There is evidence that a deep skin flora microbiome may have a role to play in post-operative infections. In this technical note, we present a skin preparation technique that addresses the issue of the skin flora beyond the initial incision. Methods : The patient is initially prepped, as standard, with. a single layer of 2% CHG+70% isopropyl alcohol. The novel stage is the 'double incision' whereby an initial superficial incision receives a further application of povidone-iodine prior to completing the full depth incision. Results : Of the 84 shunts inserted using the double-incision method (September 2015 to September 2016), only one developed a shunt infection. Conclusion : The double incision approach to skin preparation is a unique operative stage in VP shunt surgery that may have a role to play in reducing acute shunt infection.

Restoration after endodontic treatment with Endocrown (임상가를 위한 특집 3 - Endocrown을 이용한 근관치료 후 수복)

  • Park, Jeong-Kil
    • The Journal of the Korean dental association
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    • v.50 no.7
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    • pp.384-393
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    • 2012
  • Successful treatment of a badly broken down tooth with pulpal disease depends not only on good endodontic therapy, but also on good prosthetic reconstruction of the tooth after endodontic therapy is completed. The ideal treatment of endodontically treated teeth has been widely and controversially discussed. Endocrown is a restorative option for endodontically treated teeth. Endocrown design incorporates the core and short post into the crown as a single restoration. The preparation of endocrown consists of a circular equigingival butt-joint margin and central retention cavity of the entire pulp chamber instead of employing intraradicular posts. This design significantly increases the surface area of the preparation available for cementation. It is particularly useful in young patient teeth for long-term provisional restoration and in teeth with short clinical crowns. This technique represents a promising and conservative method for the treatment of endodontically treated teeth that require long-term protection and stability. Endocrown can be considered as a feasible alternative to full crowns or composite overlays for the restoration of non vital teeth.

Clinical considerations for successful results in FOS(Functional Orthognathic Surgery): Surgery first orthodontic treatment later (임상가를 위한 특집 1 - 성공적인 선 수술 교정 치료를 위한 고려사항)

  • Oh, Chang-Ok
    • The Journal of the Korean dental association
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    • v.50 no.2
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    • pp.58-63
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    • 2012
  • The fundamental principles and the role of surgeons and orthodontists to produce successful results in orthodontic treatment combined with orthognathic surgery is not different from those of conventional procedures and FOS: surgery-first-orthodontic-treatment-later approach. The communication and cooperation between surgeon and orthodontist is of crucial importance. In FOS, the pre-surgical orthodontic preparation is not carried out in the patient's mouth, but in the mounted stone model and in addition to the simulation of tooth movement, to get a precise surgical occlusion, the entire steps of treatment should be simulated on the articulator as well. Right after the surgery, due to the instability of the occlusion, appropriate post operational care should be given according to the surgical technique applied to the mandible by use of final surgical wafer about 8 weeks.

Effect of Sperm Preparation Technique on Subsequent In Vitro Development of Bovine Embryos (소 수정란 체외발달에 대한 정자분리방법의 효과)

  • 노규진
    • Journal of Embryo Transfer
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    • v.13 no.2
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    • pp.117-125
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    • 1998
  • 본 연구는 Percoll,Swim-up 그리고 Glass-wool 여과법의 세가지 정자분리 방법에 대한 정자 회수율, 생존율과 첨체반응율, 그리고 체외수정 후 배양시간에 따른 전핵형성율,수정란의 발달율과 세포할구수에 대한 효과를 조사하고자 실시하였다. 도축된 암소로 부터 채취한 난자를 22시간 체외배양 후 성숙된 난자를 체회수정시켰다. 수정 후 배양시간에 따라 존핵율을 조사하였으며 48시간에 분할율,192시간에 배반포기 발달율 및 세포할구수를 각각 비교 조사하였다. 정자의 첨체반응과 생존율은 처리군간에 차이가 없으나 회수율에 있어서 percoll 처리군이 다른 두 처리군보다 유의적으로 높았다(p<0.001).수정 후 배양시간별 전핵형성에 있어서도 percoll 처리군이 다른 두 처리군보다 빨리 진행됨을 볼 수 있다. 분할율에 있어서는 처리군간에 유의적 차이가 없으나.배반포기 발달율과 세포할구수에 있어서는 pecoll 처리군이 다른 두 처리군보다 유의적으로 높았다(P<0.05). 이상의 결과로 보아 percoll 처리에 대한 정자분리 방법은 정자 회수율이 높고 수정시 전핵형성 시간이 단축되어, 그 결과로 배반포기 발달율과 수정란의 세포할구수에 효과적임을 알 수 있었다.

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Functional Layer-by-Layer Assembled Multilayers Based on Nucleophilic Substitution reaction

  • Jo, Jin-Han
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2011.05a
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    • pp.9.2-9.2
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    • 2011
  • Ultrathin polyelectrolyte (PE) multilayer films prepared by the versatile layer-by layer (LbL) assembly method have been utilized for the preparation of light-emitting diodes, electrochromic, membrane, and drug delivery system, as well as for selective area patterning and particle surface modification because the various materials with specific properties can be inserted into the film with nano-level thickness irrespective of the size or the shape of substrate. Since the introduction of the LbL technique in 1991 by Decher and Hong, various hydrophilic materials can be inserted within LbL films through complementary interactions (i.e., electrostatic, hydrogen-bonding or covalent interaction). In this study, it is demonstrated that LbL SA multilayer films based on nucleophilic substitution reaction can allow the preparation of the highly efficient magnetic and/or optical films and nonvolatile memory devices.

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