This study was conducted to compare three commercially available egg testing devices for measuring egg quality. The devices used were a Laser-type (automatic), a Ultrasonic-type (automatic), and a Probe-type (manual). Fresh eggs weighing 60~68 grams were obtained from a commercial hen farm. Three trials were conducted. In Trial 1, a total of 50 eggs were successively analyzed by the three egg testing devices. In Trial 2, fresh eggs were successively analyzed by a combination of two egg testing devices. In Trial 3, a total of 600 eggs (weighing 60~68 grams) laid by same flock were selected, further divided into three sub-groups with a total of 200 eggs, and analyzed by an egg testing device. In Trials 1 and 2, no apparent difference was observed in egg weight between egg testing devices. However, albumin height was scored highest in the Ultrasonic-type egg tester followed by the Probe-type and Laser-type (Trials 1 and 2). Consequently, the Haugh unit was similarly altered. Yolk color was highest in the Laser-type egg tester followed by the Ultrasonic-type and Probe-type (Trials 1 and 2). When fresh eggs laid by a single flock were independently analyzed by three devices, egg weight did not differ, but albumin height and Haugh unit were higher (p<0.05) in the Ultrasonic-type egg tester than in the Probe-type or Laser-type testers. However, Laser-type testers produced higher (p<0.05) yolk color values than the Ultrasonic-type or Probe-type egg testers. In conclusion, the commercially available egg testing devices exhibited performance differences in measuring egg qualities, which warrants further consideration as to whether the magnitude of bias and precision between the devices could be acceptable in the egg grading system, especially when assessing eggs stored for certain durations.
Sorafenib is a multikinase inhibitor and an oral anticancer drug approved for the treatment of patients with advanced renal cell carcinoma and those with unresectable hepatocellular carcinoma. The purpose of this study was to develop an efficient method of the determination of sorafenib in human plasma using tandem mass spectrometry coupled with liquid chromatography (LC/MS/MS) and validate the method by the guidelines of the Korean Food and Drug Administration (KFDA). Plasma samples ($100{\mu}l$) were added with chlorantraniliprole as an internal standard and then mixed with the 0.1% formic acid-containing extraction solution composed of isopropyl alcohol and ethyl acetate (1:4, v/v). After centrifugation, the supernatant was concentrated at $45^{\circ}C$ under negative pressure and centrifugal force. The residue was reconstituted with a mobile phase and injected into the HPLC instrument using a reverse phase Waters XTerra$^{TM}$ C18 column (particle size $3.5{\mu}m$). Liquid chromatography was carried out within the run time of 5 min using a mobile phase composed of buffer (0.1% formic acid and 10 mM ammonium formate), methanol, and acetonitrile (1:6:3, v/v/v). The analytes were monitored by tandem mass spectrometry in the multiple reaction monitoring method programmed to detect sorafenib at 'm/z 465.2 ${\rightarrow}$ 252.5' and chlorantraniliprole at 'm/z 484.4 ${\rightarrow}$ 286.2' with positive electrospray ionization mode ($ES^+$). The result showed the proper linearity ($r^2$ > 0.99) over the range of 2,000-5,000 ng/ml with good accuracy (90.7-103.9%) and precision (less than 10%). The newly developed method using LC/MS/MS was validated by the guideline of KFDA and identified as more sensitive compared to the previous methods.
Ahn Seung Do;Yi Byong Yong;Choi Eun Kyung;Kim Jong Hoo;Nho Young Ju;Shin Kyung Hwan;Kim Kyoung Ju;Chung Won Kyun;Chang Hyesook
Radiation Oncology Journal
/
v.18
no.4
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pp.251-256
/
2000
Purpose : To evaluate efficacy and complication of stereotactic radiosurgery using stereotactic body frame. Methods and Materials :From December 1997 to June 1999, 11 patients with primary and metastatic tumors were treated with stereotactic radiosurgery using stereotactic body frame(Precision TherapyTu). Three patients were treated with primary hepatoma and seven with metastatic tumor from liver, lung, breast, trachea and one with arteriovenous malformation on neck. We used vacuum pillow for immobilization and made skin marker on sternum and tibia area with chest marker and leg marker. Diaphragm control was used for reducing movement by respiration. CT-simulation and treatment planning were peformed. Set-up error was checked by CT-Simulator before each treatment. Dose were calculated on the 80$\~$90$\%$ isodose of isocenter dose and given consecutive 3 fractions for total dose of 30 Gy (10 Gy/fraction). Results :Median follow-up was 12 months. One patient (9$\%$) showed complete response and four Patients (36$\%$) showed partial response and others showed stable disease. Planning target volumes (PTV) ranged from 3 to 111 cc (mean 18.4 n). Set-up error was within 5 mm in all directions (X, Y, Z axis). There was no complication in all patients. Conclusion :In Primary and metastatic tumors, stereotactic body frame is very safe, accurate and effective treatment modality.
Son, Sang Jun;Park, Jang Pil;Kim, Min Jeong;Yoo, Suk Hyun
The Journal of Korean Society for Radiation Therapy
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v.26
no.1
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pp.107-114
/
2014
Purpose : The purpose of this study is evaluation for the applicability of O-MAR(Metal artifact Reduction for Orthopedic Implants)(ver. 3.6.0, Philips, Netherlands) in head & neck radiation treatment planning CT with metal artifact created by dental implant. Materials and Methods : All of the in this study's CT images were scanned by Brilliance Big Bore CT(Philips, Netherlands) at 120kVp, 2mm sliced and Metal artifact reduced by O-MAR. To compare the original and reconstructed CT images worked on RTPS(Eclipse ver 10.0.42, Varian, USA). In order to test the basic performance of the O-MAR, The phantom was made to create metal artifact by dental implant and other phantoms used for without artifact images. To measure a difference of HU in with artifact images and without artifact images, homogeneous phantom and inhomogeneous phantoms were used with cerrobend rods. Each of images were compared a difference of HU in ROIs. And also, 1 case of patient's original CT image applied O-MAR and density corrected CT were evaluated for dose distributions with SNC Patient(Sun Nuclear Co., USA). Results : In cases of head&neck phantom, the difference of dose distibution is appeared 99.8% gamma passing rate(criteria 2 mm / 2%) between original and CT images applied O-MAR. And 98.5% appeared in patient case, among original CT, O-MAR and density corrected CT. The difference of total dose distribution is less than 2% that appeared both phantom and patient case study. Though the dose deviations are little, there are still matters to discuss that the dose deviations are concentrated so locally. In this study, The quality of all images applied O-MAR was improved. Unexpectedly, Increase of max. HU was founded in air cavity of the O-MAR images compare to cavity of the original images and wrong corrections were appeared, too. Conclusion : The result of study assuming restrained case of O-MAR adapted to near skin and low density area, it appeared image distortion and artifact correction simultaneously. In O-MAR CT, air cavity area even turned tissue HU by wrong correction was founded, too. Consequentially, It seems O-MAR algorithm is not perfect to distinguish air cavity and photon starvation artifact. Nevertheless, the differences of HU and dose distribution are not a huge that is not suitable for clinical use. And there are more advantages in clinic for improved quality of CT images and DRRs, precision of contouring OARs or tumors and correcting artifact area. So original and O-MAR CT must be used together in clinic for more accurate treatment plan.
Journal of the Society of Cosmetic Scientists of Korea
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v.33
no.2
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pp.111-115
/
2007
It is inevitable to use germicidal agents like parabens, imidazolidinyl urea, phenoxyethanol and chlorphenesin to preserve the cosmetics. Although effective in reducing microblological contamination, chemical preservatives are irritative, allergenic and even toxic to human skin. So it is needed to decrease or eliminate usage of preservatives in cosmetic products Glycerin, butylene glycol (BG), prorylene glycol (PG), and dipropylene glycol (DPG) are widely used in cosmetics as skin conditioning agent or solvents. At high concentrations, they have antimicrobial activities, but deteriorate product quality like sensory feeling or safety. The purpose of study is to evaluate the effects of polyols on antimicrobial and preservative efficacy and confirm whether using adjusted polyols can decrease the contents of preservatives without deterioration of the quality of cosmetics. Effects of common polyols on antimicrobial activities of general preservatives were measured. BG and PG significantly (p < 0.05) increased activities of preservatives, but glycerin influenced little. It was inferred from the regression analysis of the results with S. aureus that adding 1% of PG increased activities of preservatives up to $2.1{\sim}8.4 %$ and BG improved activities of preservatives up to $1.8{\sim}8.4 %$. The challenge test results for oil in water lotions and creams showed that BG and PG improved the efficacy of preservative systems up to 40 % at a range of $5.5{\sim}9.9 %$, but glycerin had little effect on it. The measured rates of improvement were analogous to the inferences from regression analysis. It can be concluded that is possible to reduce total chemical preservatives up to 40 %, consequently improve the safety and sensory quality of cosmetics with the precision control of polyols. Added to that, using this paradigm, low preservative contents, praraben-free system, and even preservative-free systems can be expected in the near future.
Journal of the Korean Association of Geographic Information Studies
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v.20
no.4
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pp.126-138
/
2017
Recently, the national interest in environment is increasing and for dealing with water environment-related issues swiftly and accurately, the demand to facilitate the analysis of water environment data using a GIS is growing. To meet such growing demands, a spatial network data-based stream network analysis map(Korean Reach File; KRF) supporting spatial analysis of water environment data was developed and is being provided. However, there is a difficulty in delineating catchment areas, which are the basis of supplying spatial data including relevant information frequently required by the users such as establishing remediation measures against water pollution accidents. Therefore, in this study, the development of a computer program was made. The development process included steps such as designing a delineation method, and developing an algorithm and modules. DEM(Digital Elevation Model) and FDR(Flow Direction) were used as the major data to automatically delineate catchment areas. The algorithm for the delineation of catchment areas was developed through three stages; catchment area grid extraction, boundary point extraction, and boundary line division. Also, an add-in catchment area delineation module, based on ArcGIS from ESRI, was developed in the consideration of productivity and utility of the program. Using the developed program, the catchment areas were delineated and they were compared to the catchment areas currently used by the government. The results showed that the catchment areas were delineated efficiently using the digital elevation data. Especially, in the regions with clear topographical slopes, they were delineated accurately and swiftly. Although in some regions with flat fields of paddles and downtowns or well-organized drainage facilities, the catchment areas were not segmented accurately, the program definitely reduce the processing time to delineate existing catchment areas. In the future, more efforts should be made to enhance current algorithm to facilitate the use of the higher precision of digital elevation data, and furthermore reducing the calculation time for processing large data volume.
This study was performed for developing upscaling Environmental Conservation Value Assessment Map(ECVAM) of National Land in South Korea and presenting the application method of ECVAM. This ECVAM adopted the least indicator method and uses a Geographic Information System(GIS). This map is made through evaluation of 67 items. As a result, the construction of ECVAM was defined as a process of identifying land use to scientifically assess the physical and environmental value of land and classify conservation value into several grades for the sustainable management of environmental resources. After applying ECVAM criteria of five degrees to the whole of study area, Grade I, showing the highest conservation value, accounted for 29.3% by land area of ECVAM. Grades II, III, IV and V likewise accounted for, respectively, 21.7%, 17.2%, 7.1% and the lowest conservation value of 24.7%. other result, ECVAM and land suitability assessment agreement rate is Grade I 33.05%, Grades II, III, IV and V likewise accounted for 12.92%, 15.05%, 36.93% and last value of 53.28% This study set up "the realization of the improvement ECVAM" as the vision of the advancing strategy. In order to accomplish the vision, this study established the purpose as follow; constructing strategic assessment value relation to ECVAM based on knowledge, arranging the foundation to upscaling assessment value And this study devised preparatory plans to achieve the vision and the purpose as next; construction on base theme map by 1:5,000 scalie, base on land register theme map and precision land cover map. Therefore, for applying the result of this study to the upscaling Environmental Conservation Value Assessment Map(ECVAM), it considers regularly the systematic categorization of preceding item, consideration issue of national environmental geographic information using the ECVAM.
Jung, Tae-Dong;Kim, Jae-Min;Choi, Sun-Il;Choi, Seung-Hyun;Cho, Bong-Yeon;Lee, Jin-Ha;Lee, Sang Jong;Park, Seon Ju;Heo, In Young;Lee, Ok-Hwan
Journal of the Korean Society of Food Science and Nutrition
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v.46
no.5
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pp.646-652
/
2017
The aim of this study was to investigate method validation for determination of sesamol, sesamin, and sesamolin in non-fermented sesame and fermented sesame by bioconversion. For validation, the specificity, linearity, precision, accuracy, limits of detection (LOD), and quantification (LOQ) of sesamol, sesamin, and sesamolin were measured by HPLC. Linearity tests showed that the coefficients of calibration correlation ($R^2$) for sesamol, sesamin, and sesamolin were 0.9999. Recovery rates of lignan contents in non-fermented and fermented sesame were high in the ranges of 100.27~115.10% and 98.43~114.90%, respectively. The inter-day and intra-day precisions of sesamin and sesamolin analyses for non-fermented and fermented sesame were 0.27~1.94% and 0.25~0.69%, respectively. The LOD and LOQ were $0.23{\sim}0.34{\mu}g/g$ and $0.70{\sim}1.03{\mu}g/g$, respectively. These results indicate that the validated method is appropriate for the determination of sesamol, sesamin, and sesamolin.
This study describes the development of a method for monitoring Aphis gossypii in greenhouse cucumber fields that was used during 2013 and 2014. The dispersion pattern of A. gossypii was determined by commonly used methods: Taylor's power law (TPL) and Iwao's patchiness regression (IPR). The sample unit was determined by linear regression analysis between mean density of sample unit versus whole plant. The optimum sample unit for different plant growth stages was two leaves (median and the lowest + 1 leaf) when the total number of leaves was less than nine, and three leaves (4th, 7th from canopy, and the lowest +1 leaf) when the total number of leaves was greater than nine. A. gossypii showed an aggregated distribution pattern, as the slopes of both TPL and IPR lines were greater than 1. TPL provided a better description of the mean-variance relationship than did IPR. The slopes and intercepts of TPL and IPR from leaf samples did not differ between the surveyed years. Fixed precision levels (D) for a sequential sampling plan were developed using Green's and Kuno's equations based on the number of aphid in a leaf sample. Green's method was more efficient than Kuno's to stop sampling. The number of samples needed to estimate the density of A. gossypii increased at higher D levels and lower mean densities. The cumulative number of aphids needed to stop sampling increased at higher D levels and with fewer plants sampled. Thus to estimate 10 aphids per leaf, 13 plants needed to be sampled, and the cumulative number of aphids to stop sampling was 131.
There is systematic spatial variations in environmental properties due to sensitive reaction to external conditions at plastic greenhouse occupied 99.2% of domestic agricultural facilities. In order to construct 3 dimensional distribution of temperature, relative humidity, CO2 and illuminance, measurement matrix as 3 by 3 by 5 in direction of width, height and length, respectively, dividing indoor space of greenhouse was designed and tested at experimental site. Linear regression analysis was conducted to evaluate optimal estimation method in terms with horizontal and vertical variations. Even though sole measurement point for temperature and relative humidity could be feasible to assess indoor condition, multiple measurement matrix is inevitably required to improve spatial precision at certain time domain such as period of sunrise and sunset. In case with CO2, multiple measurement matrix could not successfully improve the spatial predictability during a whole experimental period. In case with illuminance, prediction performance was getting smaller after a time period of sunrise due to systematic interference such as indoor structure. Thus, multiple sensing methodology was proposed in direction of length at higher height than growing bed, which could compensate estimation error in spatial domain. Appropriate measurement matrix could be constructed considering the transition of stability in indoor environmental properties due to external variations. As a result, optimal measurement matrix should be carefully designed considering flexibility of construction relevant with the type of property, indoor structure, the purpose of crop and the period of growth. For an instance, partial cooling and heating system to save a consumption of energy supplement could be successfully accomplished by the deployment of multiple measurement matrix.
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