• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.3
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• pp.215-222
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• 2006

The Lowry method was used in this study to measure protein in Haemophilus influenzae type b (Hib) conjugate vaccines (polyribosylibitol phosphate-tetanus toxoid; PRP-TT) using deoxycholic acid (DOC) to induce protein precipitation. Trichloroacetic acid (TCA) did not induce precipitation adequately from the Hib conjugate bulk and the freeze-dried Hib conjugate product. Its yield was approximately 50%. The matrix structure of Hib conjugate inhibits precipitation by TCA. Although the Lowry method can be carried out without precipitation in Hib conjugate bulk when no residual impurities (adipic acid dihydrazide [ADH], 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide-HCI [EDAC], phenol and cyanogens bromide [CNBr], etc.) are present, it cannot be used for Hib conjugate products that contain sucrose 8.5%, because 8.5% concentration of sucrose enhanced the protein concentration. DOC- and HCl-induced precipitation is an alternative method for evaluating the protein content of the Hib conjugate bulk and the Hib conjugate product. The precipitation was optimal with a final concentrate of 0.1% for DOC at $4^{\circ}C$ and pH 2. This Lowry method, using DOC/HCI precipitation to induce protein precipitation, was confirmed a consistent, reproducible, and valid test for proteins in Hib conjugate bulk and its freeze-dried product.

• Journal of Environmental Science International
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• v.27 no.12
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• pp.1227-1239
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• 2018

In this study, daily precipitation data and daily average temperature data of meteorological observatories in Daegu, Busan, Daejeon, Seoul, Mokpo, and Gwangju cities inland and offshore were analyzed by using moving average method. Were compared. Overall, summarizing changes in precipitation and temperature over the 24 seasons, precipitation and temperature in all six stations increased compared to the past 1960s. In the case of precipitation, precipitation increased at the end of July and early August, whereas precipitation in April, September and early October decreased. In the case of temperature, especially in February, the temperature increased, and in Mokpo, the temperature from August to December showed a general decline. Changes in precipitation and temperature due to seasons in the 24 seasons affect agriculture and our everyday life, and further research is needed to determine how these changes will affect agricultural water supply, crop growth and daily life. The results of this study can be useful.

• Korean Journal of Chemical Engineering
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• v.39
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• pp.3067-3073
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• 2022

This study presents the ultrasonic cavitation bubble- and gas bubble-assisted fractional precipitation that dramatically improves the precipitation efficiency in existing precipitation method for purifying (+)-dihydromyricetin. Compared to the conventional method, the time required for precipitation was reduced by 40 times. The particle size was reduced by 4.0-7.4 times and 3.7-4.4 times for cavitation bubbles and gas bubbles, respectively, and the diffusion coefficient was increased by 5.1-9.2 times (cavitation bubble) and 3.7-4.4 times (gas bubble). Meanwhile, the precipitation rate constant was increased by 11.0-65.0 times and 17.0-24.6 times and the activation energy was decreased by -5,543~-9,655 J/mol and -6,546~-7,404 J/mol, which resulted in an improved precipitation rate. The results of the thermodynamic analysis showed that the precipitation was exothermic and non-spontaneous.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.40 no.3
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• pp.265-271
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• 2020

Automotive radar that is one of the most important equipment in high-tech vehicles, is commonly used to detect the speed and range of objects such as cars. In this paper, in addition to objects detection, a method of retrieving precipitation information using the automotive radar data is proposed. The proposed method is based on the fact that the degree of attenuation of the returned radar signal differs depending on the precipitation intensity and the assumption that the distribution of precipitation is constant in short spatial and temporal observation. The purpose of this paper is to assesses the possibility of retrieving precipitation information using a vehicle radar. To verify the feasibility of the proposed method during actual driving, a method of estimating precipitation information for each time segment of various precipitation events was applied. From the results of driving field experiments, it was found that the proposed method is suitable for estimating precipitation information in various rainfall types.

• Journal of Korea Water Resources Association
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• v.55 no.12
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• pp.1053-1064
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• 2022

The Thiessen method, which is the current area average precipitation method, has serious structural limitations in accurately calculating the average precipitation in the watershed. In addition to the observation accuracy of the precipitation meter, errors may occur in the area average precipitation calculation depending on the arrangement of the precipitation meter and the direction of the heavy rain. When the watershed is small and the station density is sparse, in both simulation and observation history, the Thiessen method showed a peculiar tendency that the average precipitation in the watershed continues to increase and decrease rapidly for 10 minutes before and after the peak. And the average precipitation in the Thiessen basin was different from the rainfall radar at the peak time. In the case where the watershed is small but the station density is relatively high, overall, the Thiessen method did not show a trend of sawtooth-shaped over-peak, and the time-dependent fluctuations were similar. However, there was a continuous time lag of about 10 minutes between the rainfall radar observations and the ground precipitation meter observations and the average precipitation in the basin. As a result of examining the ground correction effect of the rainfall radar watershed average precipitation, the correlation between the area average precipitation after correction is rather low compared to the area average precipitation before correction, indicating that the correction effect of the current rainfall radar ground correction algorithm is not high.

• Journal of Korea Water Resources Association
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• v.31 no.6
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• pp.657-665
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• 1998

New diagnosing method o flood possibility was proposed. The method can be processed by following steps: first, decide if current available water resources are above normal or not; second, compute a consecutive period above normal; third, precipitation is accumulate through the period; fourth, daily depletion (runoff and evapotranspiration) amount is subtracted from the accumulated precipitation and remains are translated to one day's precipitation, which is called effective precipitation; and finally, effective precipitation index. the larger effective precipitation index means the higher flood possibility. This method has been applied to the flood event occurred in the central region of Korea at late July 1996 and compared with the study by Korea Water resources Association (1996). The new method is proven to be much faster in computation, and therefore much better in practical use for emergency situation than current rainfall-runoff models. It is because the new method simplifies some steps of currently used method such as parameter estimation and water level observation. It is also known that new method is more scientific than any other methods that use accumulated precipitation only as it considers the runoff depletion in time

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.180-180
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• 2023

To improve global risk management, understanding the characteristics and distribution of precipitation is crucial. However, obtaining spatially and temporally resolved climatic data remains challenging due to sparse gauge observations and limited data availability, despite the use of satellite and reanalysis products. To address this challenge, merging available precipitation products has been introduced to generate spatially and temporally reliable data by taking advantage of the strength of the individual products. However, most of the existing studies utilize all the available products without considering the varying performances of each dataset in different regions. Comprehensively considering the relative contributions of each parent dataset is necessary since their contributions may vary significantly and utilizing all the available datasets for data merging may lead to significant data redundancy issues. Hence, for this study, we introduce a site-specific precipitation merging method that utilizes the Quadruple Collocation (QC) approach, which acknowledges the existence of error-cross correlation between the parent datasets, to create a high-resolution global daily precipitation data from 2001-2020. The performance of multiple gridded precipitation products are first evaluated per region to determine the best combination of quadruplets to be utilized in estimating the error variances through the QC approach and computation of merging weights. The merged precipitation is then computed by adding the precipitation from each dataset in the quadruplet multiplied by each respective merging weight. Our results show that our approach holds promise for generating reliable global precipitation data for data-scarce regions lacking spatially and temporally resolved precipitation data.

• Magazine of the Korean Society of Agricultural Engineers
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• v.23 no.2
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• pp.45-53
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• 1981

Long term precipitation gaging station record (58 years) was analyzed by progressive mean method to compare the estimated effective period of records for computing mean and probable values. Obtained results are as follows: 1. Fifty-eight years precipitation records at Jinju, Gyeong Sang Nam Do was analyzed by double mass analysis method. Result was appeared that the record was consistent with time. 2. The effective period of records for estimating mean values with the departure of 5% or less from the true mean are up to 33 years for annual precipitation, 20 years for annual maximum daily precipitation and 45 years for maximum successive dry days in summer season. 3. To estimate the probable values by Gumbel-Chow method within the departure of 5% level from true value, periods of 51 years, 38 years and 45 years were required for annual precipitation, annual maximum daily precipitation and maximum successive dry days in summer season, respectively.

• Korean Journal of Environmental Biology
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• v.23 no.4
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• pp.374-378
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• 2005

A diffusion - precipitation method was developed to determine acid volatile sulfide (AVS) concentrations in freshwater sediments. This method uses silver nitrate as a sulfide trap solution and the concentration of trapped sulfide is determined gravimetrically. The proposed diffusion - precipitation method is more rapid and less expensive than previously developed purge- and - trap methods. Spiked sodium sulfide recoveries using this method $(97\~120\%)$ were similar with a previously developed diffusion - absorption method $(93.8\~115\%)$ and about $20\%$ greater than a previously developed purge-and-trap method $(74.6\~105\%)$. Detection limit of this method $(0.1\;{\mu}mole\;S\;g^{-l})$ was comparable with that of diffusion-absorption method $(0.06\;{\mu}mole\;S\;g^{-l})$ and purge-and-trap method $(0.05\~0.5\;{\mu}mole\;S\;g^{-l})$.

• Journal of Environmental Science International
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• v.28 no.10
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• pp.841-849
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• 2019

The purpose of this study was to evaluate the method of estimating the areal precipitation reflecting the altitude of the mountainous terrain on Jeju Island by comparing and analyzing the areal precipitation using the Thiessen polygon method and the isohyetal method in mountainous streams. In terms of constructing the Thiessen polygon network, rainfall errors occurred in 94.5% and 45.8% of the Thiessen area ratio of the Jeju and Ara stations, respectively. This resulted in large areal precipitation and errors using the isohyetal method at altitudes below 600 m in the target watershed. In contrast, there were small errors in the highlands. Rainfall errors occurred in 18.91% of the Thiessen area ratio of Eorimok, 2.41% of Witseoreum, and 2.84% of Azalea Field because of the altitudinal influence of stations located in the highlands at altitudes above 600 m. Based on the areal precipitation estimation using the Thiessen polygon method, it was considered to be partially applicable to streams on Jeju Island depending on the altitude. However, the method is not suitable for mountainous streams such as the streams on Jeju Island because errors occur with altitude. Therefore, the isohyetal method is considered to be more suitable as it considers the locations of the rainfall stations and the orographic effect and because there are no errors with altitude.