• 제목/요약/키워드: ppGpp

검색결과 21건 처리시간 0.026초

Initial Characterization of yliH in Salmonella typhimurium

  • Park, Kyung-Hwa;Song, Mi-Ryung;Choy, Hyon-E.
    • Journal of Microbiology
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    • 제45권6호
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    • pp.558-565
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    • 2007
  • Using microarray analysis, we determined those Salmonella genes induced at the entry of stationary phase, and subsequently discovered that uncharacterized yliH was induced most dramatically. We set out to establish the molecular mechanism underlying the stationary phase induction of yliH under the standard culture condition, LB with vigorous aeration, by analyzing its promoter activity in various mutant backgrounds, lacking stationary phase ${\sigma}$, $RpoS^-$, or stringent signal molecules ppGpp, ${\Delta}relA$ ${\Delta}spoT$. It was found that the stationary phase induction of yliHp was partially dependent on rpoS but entirely dependent on ppGpp. DNA sequence analysis revealed that the Salmonella yliH gene is composed of 381 base-pair nucleotides, with overall amino acid sequence revealing 76.38% amino acid identity and 88.98% similarity with Escherichia coli yliH, although no motif from data base was noted for its possible role. Recently however, it has been reported that yliH in E. coli was implicated in biofilm formation and motility by repressing these activities (Domka et al., 2006). We have constructed a mutant Salmonella deleting yliH gene by allele replacement and examined its phenotype, and found that the yliH in Salmonella more or less affects motility and adherence by enhancing these activities. The effect on biofilm formation in Salmonella was uncertain. Moreover, addition of cloned yliH of E. coli into Salmonella did not reduce motility or adherence. Taken together, it appears that the pathways implicating yliH for biofilm formation and motility in E. coli and in Salmonella are somewhat different.

유청 단백질에서 유도되는 생리활성 펩타이드에 관한 연구 (Isolation of Mitogenic Glycophosphopeptides from Cheese Whey Protein Concentrate)

  • 윤숭섭
    • Journal of Dairy Science and Biotechnology
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    • 제15권1호
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    • pp.33-44
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    • 1997
  • 1. CWPC중의 새로운 생리활성물질의 검색 Mouse 임파세포의 증식효과를 지표로 하는 면역기능을 검토하여 CWPC중의 면역 부활작용을 갖는 새로운 성분의 검색을 실시하였다. CWPC를 여러 가지 분획법으로 분획하여 mouse 임파세포의 증식효과를 지표로 면역 활성성분을 검색하였다. 그 결과 gel filtration, 음이온교환법을 사용하여 분획한 당을 다량 포함한 부분에 강한 면역 부활담당세포에 대하여 증식활성을 나타내는 물질을 발견하였다. 이 물질은 SDS-PAGE상에서 분자량이 약 16kDa에 위치하여 Ca, p및 당쇄를 포함한 물질이며, 이것을 GPP로 하였다. GPP에는 우유케이신의 trypsin분해물이며 Ca와 무기인을 풍부하게 포함하는 ${\beta}$-CPP와 유사한 phosphoserin 영역을 갖는 성분과 갖지 않는 성분의 2종류가 존재하며, 각각의 면역 부활활성이 인정되었다. 각 성분의 아미노산 분석, 당 분석의 결과에서 지금까지 보고된 우유중의 면역담당세포에 대한 증식활성을 갖는 물질과는 상이한 성분인 것으로 밝혀졌다. 더우기 이 활성물질(GPP)은 PP cell에서도 동등한 활성이 있는 것으로 판단되었다. 이러한 결과를 종합하여 보면 CWPC중에는 지금까지 알려지지 않았던 새로운 면역 부활물질이 존재하며, 그 성분에는 CPP와 유사한 phophoserine영역이 존재하는 성분이 포함되어 있고, N-글리코실 결합의 당쇄가 존재하는 것으로 시사되었다. 이 성분은 전신면역의 지표인 비장세포에 대해서만이 아니고, 장관면역계에 중요한 역할을 담당하는 PP Cell에서도 활성이 있는 것으로 보아 전신 및 국부적인 면역기능의 부활성분으로서 응용의 가능성이 시사되었다.

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SPECIAL Edition - [HACCP 기준원 기획연재] 선행요건프로그램의 작성

  • 이경순
    • 오리마을
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    • 통권83호
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    • pp.45-46
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    • 2010
  • 농장에서 효과적인 HACCP시스템의 적용을 위해서는 HACCP을 적용함에 있어서 토대가 되는 "선행요건프로그램(Pre-requisite program, PP)" 즉, 자체위생관리기준(Sanitation Standard Operating Procedures, SSOP)과 우수 농장관리기준(Good Agricuiture / Production Practice, GAP/GPP)이 적절히 운영되어야 한다는 것을 지난호에서 기술한 바 있다. 이번호부터 선행요건프로그램에 대해서 좀 더 자세히 알아보기로 하자.

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Stringent Factor Regulates Antibiotics Production and Morphological Differentiation of Streptomyces clavuligerus

  • RYU , YONG-GU;JIN, WOOK;KIM, JIN-YOUNG;KIM, JAE-YOUNG;LEE, SANG-HEE;LEE, KYE-JOON
    • Journal of Microbiology and Biotechnology
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    • 제14권6호
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    • pp.1170-1175
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    • 2004
  • The involvement of the relA and rsh genes in the morphological and physiological differentiation of Streptomyces clavuligerus was evaluated with the relA and rsh genes mutants. The morphological differentiation of S. clavuligerus was greatly affected by the disruption of the relA gene, but not very much by the disruption of the rsh gene. The altered morphological characteristics were completely restored by the complementation of the corresponding disrupted genes. Thus, it was apparent that the mycelial morphology and clavulanic acid production were severely affected by the disruption of the relA gene. Production of clavulanic acid in the submerged batch culture and glycerol-limited chemostat showed that production was inversely related to the specific growth rate in the wild-type strain. However, the production of clavulanic acid in the ${\Delta}relA$ and ${\Delta}rsh$ null mutants was completely abolished. Therefore, it seems plausible that the stringent response of S. clavuligerus to starvation for amino acids is governed mainly by ReIA, rather than Rsh, and that the (p)ppGpp synthesized immediately after the depletion of amino acids triggers the initiation of pathways for both morphological and physiological differentiation in this species.

국제표준화기구의 표준화활동 정책동향 (ITU-PP 활동을 중심으로) (ITU Plenipotentiary Conference)

  • 이동철
    • 한국정보통신학회:학술대회논문집
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    • 한국해양정보통신학회 2008년도 추계종합학술대회 B
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    • pp.917-918
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    • 2008
  • 국제표준화기구의 각 국의 표준화활동 정책동향 연구를 통하여 각 국이 왜 의장단에 진출하여 활동할려고 하는지, 이로 인한 자국의 이익이 무엇인지, 기술 반영은 어느 정도 되고 있는지, 표준화의 주도적 역할을 과연 의장단 진출로 인하여 반영은 할 수 있는지 등에 대해 논의하고자 한다. 우리나라는 지난 1987년이후 의장단 진출을 위해 국제표준화기구의 연구위원회 회의 등을 한국에서 수차례 유치하는 등 정부의 많은 노력 끝에 국내에서 그동안 각 분야별 전문가들이 국제기구에의 의장단에 진출하여 표준화 활동을 하고 있다. 지난 터키 회의에서 ITU-T 사무총장 진출을 하였으나, 좌절되었고 최근 소련에서 개최된 3GPP에서 한국이 부의장에 당선되는 등 최근의 의장단 활동이 두드러지게 나타나고 있다. 이번 WTSA에서는 25명의 의장단이 진출할 예정이다. 이로 인하여 향후 표준화활동의 지속적인 활동으로 정보통신 분야에서의 기술적 이익이 반영되었으면 한다.

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Hfq and ArcA Are Involved in the Stationary Phase-Dependent Activation of Salmonella Pathogenicity Island 1 (SPI1) Under Shaking Culture Conditions

  • Lim, Sangyong;Yoon, Hyunjin;Kim, Minjeong;Han, Ahreum;Choi, Jihae;Choi, Jeongjoon;Ryu, Sangryeol
    • Journal of Microbiology and Biotechnology
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    • 제23권12호
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    • pp.1664-1672
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    • 2013
  • In Salmonella enterica serovar Typhimurium, many genes encoded within Salmonella pathogenicity island 1 (SPI1) are required to induce intestinal/diarrheal disease. In this study, we compared the expression of four SPI1 genes (hilA, invF, prgH, and sipC) under shaking and standing culture conditions and found that the expression of these genes was highest during the transition from the exponential to stationary phase under shaking conditions. To identify regulators associated with the stationary phase-dependent activation of SPI1, the effects of selected regulatory genes, including relA/spoT (ppGpp), luxS, ihfB, hfq, and arcA, on the expression of hilA and invF were compared under shaking conditions. Mutations in the hfq and arcA genes caused a reduction in hilA and invF expression (more than 2-fold) in the early stationary phase only, whereas the lack of ppGpp and IHF decreased hilA and invF gene expression during the entire stationary phase. We also found that hfq and arcA mutations caused a reduction of hilD expression upon entry into the stationary phase under shaking culture conditions. Taken together, these results suggest that Hfq and ArcA regulate the hilD promoter, causing an accumulation of HilD, which can trigger a stationary phase-dependent activation of SPI1 genes under shaking culture conditions.

WLAN-3GPP Integration Architectures for Packet Based Data Services

  • Raktale Swapnil K.;Kumar Ashok
    • 정보통신설비학회논문지
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    • 제2권3호
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    • pp.48-60
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    • 2003
  • With the rapid successful deployment of WLANs worldwide in numerous hotspots for high data rate, wireless access for data services has created the need to integrate the Cellular Networks with WLAN Networks. The integrated wireless environment will provide the end user a much better service experience along with a single subscription and a single bill. In this paper we focus on inter-working approaches, which combine WLANs and Cellular Networks into an integrated wireless environment capable of ubiquitous access to data services and very high data rates in hotspots areas. We first list the key requirements which are currently being standardized within the 30PP for integration with WLANs networks. We discuss two inter-working architectures namely loosely coupled and tightly coupled This paper will detail the loosely coupled inter-working approach while briefly discussing the tightly coupled inter-working. Finally, we will conclude that the loosely coupled approach is evolutionary and less intrusive than the tightly coupled approach.

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