• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.265-269
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• 1999

Transgenic tobacco plants expressing either a sweet potato anionic peroxidase (POD) (swpal) or neutral POD (swpnl) were irradiated by gamma radiation, and the gamma radiation-induced biochemical changes in antioxidant enzymes and plant growth inhibition were investigated at 30 days after treatment. Gamma radiation significantly inhibited the growth of all plants regardless of transgenic or nontransformed plants, showing a dose-dependent inhibition. In high dosage of 50 and 70 Gy, plant heights were severely retarded and new leaves does not emerged. No significant changes in antioxidant enzymes such as POD, superoxide dismutase and catalase were observed in all plants regardless of irradiation dosages ranging from 10 to 50 Gy. These results suggest that sweet potato PODs may be not involved in the protection against the oxidative stress induced by gamma radiation.

• Korean journal of applied entomology
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• v.16 no.3 s.32
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• pp.145-148
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• 1977

he study was conducted to produce an antiserum of potato virus S for identification and screening of seed-potatoes. Potato virus S was isolated from infected plants and identified by means of indicator plants and electro microscopy. Isolated potato virus S was multiplied in Nicotiana deebneyii and the virus was purified by a modified method that was developed through this study. The purity of potato virus S was 1.18mg/ml. Purified potato virus S was injected into rabbit intravenously once a week for 5 weeks. Antiserum was collected 10 days after the last injection. The produced antiserum was determined to have a titer of, 1/2048 by means of microprecipitin tests.

• Journal of the Korean Society of Tobacco Science
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• v.20 no.1
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• pp.57-65
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• 1998

This study was conducted to develop a resistant tobarro against Potato virus Y (PVY) by transformation of the plants with genetically engineered viral genes. The complimentary DNAs (cDNAS) of potato virus Y-necrosis strain (PVY-Vn) replicase mutant genes (3'-deleted, 5'-deleted and ADD-mutant Nlbs) were synthesized through RT-PCR by using purified PVY-VN RNA and synthesized primers, and cloned in the sense orientation into a plant expression vector (pMBPI), The cDNAS of the genes were transferred into Agrobacterium tumefaciens LBA 4404, and then transformed into tobacco (Nicotiana tabacum cv. Burley 21) plants. Regenerated plants were tested for PVY resistance by inoculation test; 13 transgenic plants including 7 for 3'-deleted Nlb, 3 for 5'-deleted Nlb, and 3 for ADD-mutant Nlb appeared to be resistant at 4 weeks after inoculation with PVY-VN. Among the 13 transgenic tobacco plants, 8 plants had no symptom up to 14 weeks after inoculation. The progenies ($T_1$) from self-fertilization of the transgenic lines varied 0.0% to 81.2% in their resistance (% of resistant plants). The analysis of Nlb-31deleted, -5'deleted and -ADD mutant in the $T_1$ plants by polymerase chain reaction (PCR) showed that Nlb-3'deleted, -5'deleted and -ADD mutants were detected in all of the resistant plants. These results suggest that the PVY resistance was inherited in the $T_1$ generation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.4
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• pp.498-504
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• 2014

Potato virus Y (PVY) and potato leafroll virus (PLRV) are among the most damaging potato viruses and prevalent in most potato growing areas. In this study, cryopreservation was used to eradicate PVY and PLRV using two cryogenic methods. Potato shoot tips proliferated in vitro were cryopreserved through droplet-vitrification and encapsulation-vitrification using plant vitrification solution 2 (PVS2; 30% glycerol + 15% dimethyl sulfoxide + 15.0% ethylene glycol + 13.7% sucrose) and modified PVS2. Both cryogenic procedures produced similar rates of survival and regrowth, which were lower than those from shoot tip culture alone. The health status of plantlets regenerated from shoot tip culture alone and cryopreservation was checked by reverse transcription-polymerase chain reaction. The frequency of virus-free plants regenerated directly from highly proliferating shoot tips reached 42.3% and 48.6% for PVY and PLRV, respectively. In comparison, the frequency of PVY and PLRV eradication after cryopreservation was 91.3~99.7% following shoot-tip culture. The highest cryopreserved shoot tip regeneration rate was observed when shoot tips were 1.0~1.5 mm in length, but virus eradication rates were very similar (96.4~99.7%), regardless of shoot tip size. This efficient cryotherapy protocol developed to eliminate viruses can also be used to prepare potato material for safe long-term preservation and the production of virus-free plants.

• Journal of the Korean Home Economics Association
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• v.42 no.4
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• pp.187-194
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• 2004

The concentration of potato(Solanum tuberosum L.) glycoalkaloids(PCA) (i.e., ${\alpha}$-chaconine and ${\alpha}$-solanine) in Korean cultivated potato plant, and in the different varieties, parts and weights of the potato tubers, were determined by high-performance liquid chromatography(HPLC). The highest concentrations of PGA in potato plants were found in the roots, followed by the stems and leaves. A large quantity of PGA existed in the periderm of 'Atlantic' potatoes, whereas 'Irish Cobbler' contained the lowest amount in five cultivated potato tubers.'Irish Cobbler' potatoes were divided into three parts and the PGA content in each part was determined. It was found that both of the end parts of the potatoes contained higher PGA than the middle part. The PGA contents in four different sizes of potatoes increased toward the smaller size. Thesignificantly high level of PGA was contained in the smallest size potato tubers, which have been used widely for Korean cooking.

• Journal of Plant Biology
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• v.15 no.1
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• pp.23-27
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• 1972

A total of 163 virus infected pepper plants(Capsicum annuum L.) collected from various pepper growing regions in Korea were investigated on the presence of tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), potato virus X(PVX), potato virus Y(PVY) and alfalfa mosaic virus (AMV) by serological methods. Van Slogteren's microprecipitin test was applied for the testing of TMV, PVX and PVY from infected plants, and Ouchterlony agar double diffusion test was used for CMV and AMV. Results obtained are as follows: 1. TMV, CMV, PVX, PVY and AMV were found to occur on the pepper plants growing in Korea. 2. The prevalence of each of these viruses among the 163 pepper plants investigated was in the order of CMV: 93 plants(57.0%)>TMV: 91 plants (55.8%)>AMV: 58 plants (35.6%)>PVY: 40 plants (24.5%)> PVX:6 plants(3.7%). 3. Among the 163 plants investigated, 72 plants (44%) showed infection with one kind of virus and 91 plants (56%) showed mixed infection with more than two different viruses. In general, heavier damage of the plants was observed from mixed infection. 4. The results of serological identification of pepper viruses coincided with those results obtained by sap inoculation experiment conducted at the Horticultural Experiment Station along with present investigation. Thus the serological techniques applied in this experiment proved to be very reliable for the identification of TMV, CMV, PVX, PVY and AMV from pepper plants infected with these viruses.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.425-425
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• 2005

• Journal of Plant Biology
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• v.30 no.4
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• pp.287-298
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• 1987

The regenerative capacities of protoplasts isolated from potato (Solamum tuberosum L.) tubers and tobacco (Nicotiana tabacum L.) mesophyll tissues were examined, and then their intergeneric protoplast fusion was carried out. The potato tuber-derived protoplasts proliferated into the calli some of which showed rudimentary shoot-like structures, which had not been attempted before from tubers, while the tobacco protoplasts were regenerated into the whole plants. Intergeneric protoplast fusion between potato and tobacco was carried out and the heteroplasmic fusion products were formed. The first cell division of some of them was observed after 5 days of culture.

• Mycobiology
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• v.35 no.2
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• pp.97-99
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• 2007

Specimens collected from sweet potato plants with slime mold symptoms in fields in Daejeon, Korea were examined. Two species of Myxomycetes, Fuligo septica and Stemonitis herbatica were identified based on their morphological characteristics. This is the first report that the two species of Myxomycetes cause slime mold of sweet potato in Korea.

• Korean Journal of Environmental Agriculture
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• v.9 no.2
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• pp.121-131
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• 1990

Four crop plants, such as green onion(Allium ascalonicum L.), radish(Raphanus sativus L.), potato(Solanum tubersum L.) and chinese cabbage(Brassica pekinensis Rupr), were grown in greenhouses to determine the accumulations of Cd and Zn absorbed by the plants grown on soils treated with municipal sewage sludge of different levels of 0, 22.5, 45.0 and 90.0 ton $ha^{-1}$ as soil dry weight. The result was analyzed to be comparable to the heavy metal content in the plant tissues among the crop species and the statistical characteristics were investigated to determin a possible relationship between causes of increased contents of Cd and Zn in plants and in soils. The results were as follows : 1. The Cd and Zn content in experimental crop plants increased with increase in quantity of treatment sludge. 2. The Cd and Zn content in roots of all experimental plants increased with sludge treatment amount in soil, in the following order : green onion>potato>chinese cabbage>radish. 3. The Cd content in leaves of crop plants were high in this order : green onion>radish>potato>chinese cabbage, and the Zn content in leaves of crop plants was high in this order. : potato>radish>chinese cabbage>green onion. 4. Concentrations of Cd in potato tubers, radish roots and chinese cabbage leaves ranged from 0.03 to 0.12 ppm. 5. Concentrations of Cd and Zn in plant tissues grown in sludge treated soil of 90.0 ton $ha^{-1}$ were 1.2 to 10 times and 0.4 to 2.3 times higher as compared with those absorbed from sludge-free soil, respectively. 6. The accumulation rates of Cd and Zn in sludge-treated plant tissue were almost constant except for green onion roots.