• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.337-343
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• 2013

Post-thawed larval rearing in Pacific oyster Crassostrea gigas was performed to investigate the survival rate with time course in three kinds of larvae cryopreserved. The highest survival rate and larval activity index (LAI) of post-thawed larvae were obtained from the permeation in 0.2 M sucrose and 2.0 M ethylene glycol (EG) at $-1^{\circ}C/min$ in freezing speed showing the survival rates just after thawing of 63.8% in trochophore, 84.1% in D-shaped veliger and 56.3% in early umbo veliger. In post-thawed larval rearing with food supply, the larvae lasted their lives until 24 hours in trochophore, 75 hours in D-shaped veliger and 57 hours in early umbo veliger. The results suggested that each larval stage post-thawed revealed no more further development to subsequent respective stage.

• 한국발생생물학회지:발생과생식
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• 제18권4호
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• pp.241-249
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• 2014

Hyphessobrycon eques is a famous fish for ornamental fish market and aquarium. They are inhabit in regions of Amazon and Paraguay River basin. Serpae fishs were investigated 2-3 males are chased to female, and then males attempted to simulate the females abdomen. After fertilization, eggs were kept in incubators at $28^{\circ}C$. The fertilized eggs had adhesive and demesal characteristics and had a mean diameter of $0.92{\pm}0.01mm$. Larvae hatched at 16 hrs post fertilization. The hatched larvae averaged $2.90{\pm}0.16mm$ in total length ($L_T$). Complete yolk sac resorption and mouth opening occurred on the third day post hatching. At 45 days post hatching, the larvae were $12.5{\pm}1.60mm$ $L_T$ and had reached the juvenile stage.

• 한국양봉학회지
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• 제33권4호
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• pp.283-295
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• 2018

As Sacbrood virus (SBV), a causative agent of larval death and colony collapse in Apis cerana honey bee, is prevalent and poses one of the most significant threats to the Korean apiculture, development of methods to counter this viral disease is urgently needed. In this study we tested some SBV controlling methods, such as requeen, shook swam, adding Apis mellifera and spraying yogurt to SBV inoculated colony. Colony size measured by number of sealed brood and adult were evaluated every 15 days until two months while instances of recurrence were recorded up to five months after applying treatment methods. We also test the effects of yogurt on healthy and SBV-infected larvae at both in vitro reared larvae and colony level. Our result showed that all SBV controlling methods had similar success rates with respect to elimination of SBV clinical symptom up to 30 days post treatment. Mix-species and spraying yogurt method had similar pattern of sealed brood and adult number and higher than that of other SBV controlling methods up to 45 days post treatment. These two groups also showed the lower percentage of SBV recurrence (50% and 66.7%) at 120 days post treatment than other group that mostly colony had clinical symptom. Result on in vitro reared larvae challenged with yogurt showed that yogurt have neither harmless on healthy larvae nor remedial effect on SBV infected larvae. However, at colony level, colony in group received yogurt treatment removed significantly more SBV-infected larvae, SBV-killed larvae, and even healthy larvae in comparison to the control, suggested that yogurt could trigger the hygienic behavior of nurse bee. Our results recommended that it is practical in beekeeping by adding A. mellifera and spraying yogurt to control SBV in A. cerana colony.

• International Journal of Industrial Entomology and Biomaterials
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• 제15권1호
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• pp.9-16
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• 2007

The present study was aimed to screen different antibiotics in vivo for their effects against the bacterium Bacillus coagulans in silkworm Bombyx mori in three experimental conditions viz., a) healthy larvae treated with the antibiotics b) larvae which were first inoculated with Bacillus coagulans immediately after the $2^{nd}$ moult and treated with the antibiotics from 24 hour after inoculation up to the end of the $5^{th}$ instar(pre-inoculated larvae) and c) larvae which were treated with the antibiotics right from hatching up to the end of the $5^{th}$ instar and inoculated with Bacillus coagulans immediately after the $2^{nd}$ moult(post-inoculated larvae). All the antibiotics used in this study stimulated better performance in improving the rearing performance and economic characters of the cocoons in healthy, pre-inoculated and post-inoculated larvae, besides reducing the mortality of infected larvae. Cephelexin and Tetracycline were more effective than the others in their overall performance under all the three experimental conditions. Chloramphenical caused greater increase in the length of the reelable silk filament, but was not as good in elevating the other parameters compared to the remaining antibiotics. Of the four concentrations of antibiotics tested, 2.0% performed better under all the three experimental conditions.

• 한국수산과학회지
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• 제7권2호
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• pp.52-62
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• 1974

1. 수조내에서 순화된 친가에서 부화시킨 유생을 실험실내에서 수온 $27.5\~28.7^{\circ}C$, 염분 $6.58-7.05\%_{\circ}$Cl. pH $8.0\~8.2$, 조도 3000lux, 여과수양 $0.3{\ell}/min$의 조건하에서 먹이로는 Artemia salina nauplius를 참여하여 사육하였다. 2. 갓 부화된 zoea 유생의 체장은 1.92mm이며 제1 post-larva는 7.69mm로써 11영기의 zoea기에서 제1 post-larva로 변태하며 상기 조건하에서는 35일이 소요되며 제11 영기에서 제1 post-larva로 변태할 때 복절의 길이가 줄어들고 굵어진다. 3. 갓 부화된 유생의 제3 복절의 배면에 출현하는 3개의 색소포와 미절의 기부에 나타나는 1개의 색소포는 Palaemonidae속 새로운 분류지표로 중요하다. 4. 유생의 제6 영기 부터 개체변이체인 "숙성이"가 출현하며 동일 영기개체군에서 형태적으로 뛰어나게 크다. 5. 유생의 전발생영기를 통하여 특히 "숙성이"가 출현하기 쉬운 단계가 있는 것 같으며 이것은 제6영기와 제10영기인 것 같다. 이 현상은 체장의 변동폭의 넓이와 관계가 크며 이것은 섭이요인이 가장 크게 작용하는 것같다. 6. 유생의 발생도중 완전한 skipping 현상이 나타나는 것처럼 여겨졌으나, 영기별 각 부속기관의 형태적인 특징을 비교관찰한 결과, 어느 영기가 완전히 skipping하는 것은 아닌 것 같다. 7. "숙성이"의 부속기관을 비교검토한 결과, 한영기에서 다음 영기로 분화발생할 때 각 기관의 분화가 동시에 이루어지는 것이 아니고, 어떤 연관성을 가지며 각 기관마다 개별적인 분화발생속도를 유지하며 분화의 동시성은 볼 수 없는 것 같다.

• 한국발생생물학회지:발생과생식
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• 제21권1호
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• pp.63-69
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• 2017

On the 15 days after hatching, the larvae was 4.24-5.10 mm (mean $4.66{\pm}2.18mm$) in total length, and the fins of the membrane started to develop into a fan shape and the melanophore was deposited upper the alimentary canal of the abdomen and on the bladder. At 35 days after hatching, the post-larvae formed a branch-shaped melanophore on the head part with a total length of 6.98-12.5 (mean $9.35{\pm}1.71$) mm, formed on the upper and lower parts of the caudal part, formed on the upper and lower parts of the caudal part, and deposited under the head part and abdomen. At 40 days after hatching, the juvenile was 11.3-18.1 (mean $14.9{\pm}1.53$) mm in total length.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.76-77
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• 2003

Olive flounder Paralichthys olivaceus larvae and early juveniles were reared for 43 days after hatching in order to observe the effects of starvation during development and metamorphosis. Morphological, histological and biochemical measurements were made to assess the nutritional condition during growth and starvation from pre-matamorphic through post-metamorphic phases. Two groups of fish were compared ; one with sufficient food supply and one under continuous starvation until death. Among morphometric analyses, both ratios of body height at anus/head height and pre-/post-anal lengths appeared to be sensitive to starvation during which substantial reduction was observed within a day of food deprivation. Histological variables as intestinal and rectal epithelial heights and gall bladder volume changed significantly with onset of starvation. The gut epithelial heights of starving fish decreased with advances in starvation, although they fluctuated during mid-metamorphic phase. In contrast, gall bladder volume increased remarkably soon after starvation. Ontogenetic changes in both gut epithelial height and gall bladder volume were evident, those associated with settlement and/or completion of metamorphosis. Abrupt decrease in the RNA/DNA ratios of starving fish were found right after onset of starvation. Even in the fed fish marked fluctuations in its ratios during metamorphosis were observed, evident by decreasing from late-metamorphic to post-metamorphic stages. These findings suggest that a combination of morphologically and histologically sensitive characteristics, and biochemical measurement could be utilized as a measure to evaluate nutritional condition related to starvation in wild olive flounder larvae and juveniles.

• Parasites, Hosts and Diseases
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• 제37권2호
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• pp.101-107
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• 1999

Rats were immunized through an initial infection with 1,000 filariform larvae (L3) of Nippostrongylus brasiliensis and after complete expulsion of worms they were challenged with 1,000 L3 of Strongyloides venezuelensis to investigate whether cross-resistance developed against a heterologous parasite. Nippostrongylus brasiliensis immunized rats developed a partial cross-resistance against S.venezuelensis migrating larvae (MSL3) in the lungs and adult worms in the small intestine. The population of MSL3 in the lungs were significantly lower (p<0.05) in immunized rats($22.0{\;}{\pm}{\;}7.4$) compared with controls ($105.0{\;}{\pm}{\;}27.6$). The populations of adult worms, egg output and fecundity were initially decreased but from day 14 post-challenge they did not show any significant difference between immunized and control rats. However, the length of worm in immunized rat was revealed as retardation. Peripheral blood eosinophilia was significantly decreased (P<0.05) on day 7 post-challenge and then gradually increased which peaked on da 42 post-challenge when most of the worms were expelled. these results suggest that peripheral blood eosinophilia is strongly involved in the worm establishment and expulsion mechanisms.

• BMB Reports
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• 제41권8호
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• pp.587-592
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• 2008

Cotesia vestalis is an endoparasitoid of Plutella xylostella larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we characterize the gene, CvBV3307, and its products. CvBV3307 is located on segment S33 of the CvBV genome, is 517 bp, and encodes a putative protein of 122 amino acids, including a serine-rich region. The expression pattern of CvBV3307 in parasitized larvae and the subcellular localization of CvBV3307 only in granulocytes indicated that it might be involved in early protection of parasitoid eggs from host cellular encapsulation and in manipulating the hormone titer and developmental rhythm of host larvae. Western blot analysis showed that the size of the immunoreactive protein (about 55 kDa) in parasitized hosts at 48 hours post parasitization (h p.p.) is much larger than the predicted molecular weight of 13.6 kDa, which suggests that CvBV3307 undergoes extensive post-translational modification in hosts.

• 한국잠사곤충학회지
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• 제35권1호
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• pp.69-72
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• 1993

Testosterone Propionate가 가잠의 실용형질에 미치는 영향 다화성계통의 1종인 Pure Mysore 품종에 대한 testosterone propionate(TP)의 농도별 (1, 5, 10$\mu$g/ml) 투여효과를 조사하였다. TP의 처리는 유충 3, 4, 5령의 36시간째 투여구(처리-1), 4, 5령의 48시간째 투여구(처리-2) 및 5령 72시간째 투여구(처리-3)의 3개 시험구를 설정하여 농도별로 피부에 침투하였다. 실험 결과 모든 시험구에서 TP 처리는 산란성에 증대효과를 보였으나, 화용비율 및 발아비율은 대조구에 비해 떨어지고 있었다.