• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1997년도 춘계학술대회
• /
• pp.81-81
• /
• 1997

We previously demonstrated an enhancer-like positive regulatory element within a 259-bp sequence (-2352 to-2094 bp) of the human CYP1A2 gene in HepG2 cells. Three protein binding sites were identified by DNase I footprint analyses within the 259-bp sequence: protected region A PRA ( -2283 to-2243 bp), PRB (-2218 to-2187 bp), and PRC (-2124 to-2098 bp) (I. Chung and E. Bresnick, Mol. Pharmacol. 47, 677-685, 1995). In the present study, the functional significance of those protected regions was examined. Transfection experiments with deletion and substitution mutants defined the PRB and PRC as containing positive and negative regulatory elements, respectively. Human breast carcinoma MCF-7 cells were cotransfected with a hepatocyte nuclear factor-1 (HNF-1) expression vector and CYP1A2 promoter-or thymidine kinase promoter-luciferase remoter gene constructs. HNF-1, which contributes to the liver specificity of genes, enhanced reporter gene activity in a PRC sequence-dependent manner. These results suggested that PRC could exist bound to a repressor which was displaceable by other transcription factors such as HNF-1. Results obtained by transfection of HepG2 hepatoma cells with various PRB substitution mutant-luciferase gene fusion constructs indicated that the entire sequence of PRB was necessary for promoter activity. Consequently, the regulation of CYP1A3 expression is very complex, requiring a number of both positive and negative regulatory factors.

• 동아시아경상학회지
• /
• 제10권3호
• /
• pp.63-74
• /
• 2022

Purpose - The purpose of this study is to set up the elements of the airline marketing communication and find out the effect of each element on brand trust. It also aims to figure out how brand trust affects the customer's repurchase intention and WOM intention respectively, and whether repurchase intention has an influence on WOM intention. Eventually, this research intends to provide not only academic but practical application of the research by figuring out the marketing communication factors. Research design, data, and methodology - The survey for this study has been conducted for about 2 months, targeting those who have flown with either domestic or foreign airlines. The number of 249 surveys were distributed and the total number of specimens is 244. Data analysis methods were verifiable factor, discriminant, nomological, and structural equation model analysis. Result - Only advertisement, promotion and WOM have a positive effect on brand trust while publicity has no impact on brand trust. Brand trust was found to have a positive effect on repurchase intention. Also Brand trust also has a positive effect on WOM intention. Finally repurchase intention has a positive effect on WOM intention. Conclusion - There are insufficient existing studies of airline marketing communication which has the direct influence on brand trust. Therefore, the study provided the theoretical foundation of airline marketing communication elements that directly effect on airline brand trust.

• Parasites, Hosts and Diseases
• /
• 제55권6호
• /
• pp.607-611
• /
• 2017

Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA. According to the results, 53.5% of the women under study were positive for anti-Toxoplasma antibodies: 4.0% of them had IgM, 43.0% had IgG, and 6.5% had both IgM and IgG. Subsequently, Nested-PCR analysis was used to detect T. gondii DNA in the placenta of subjects. In 10.5% of the women, the results were positive for 529 bp element of T. gondii. Among them, 5 (23.8%) cases were IgM positive, 1 (4.8%) case was IgG positive, and 11 (52.4%) were both IgM and IgG positive. In 4 (19.0%) patients, none of the antibodies were found to be positive. In total, 16 patients had positive results in both ELISA and PCR methods, and 174 cases had negative results for new infection. The findings of this study revealed that T. gondii might be one of the significant factors leading to abortion, and that the analysis of placenta can be important in order to achieve increased detection sensitivity.

• 한국항공우주학회지
• /
• 제33권11호
• /
• pp.7-14
• /
• 2005

접촉 현상을 포함하는 완전 연계된 열기계학적 문제에 대한 정확하고 효율적인 해석을 위하여 부영역과 공유면에 근거한 유한요소 정식화 기법을 제안하였다. 부영역과 공유면을 결합하기 위한 등식 적합 조건을 벌칙 함수로 처리함으로써 모든 유효 강성 행렬이 양정치화되며, 역행렬과 같은 각종 수치 연산이 매우 간편하다. 또한 전체 구조 형상이 복잡하더라도 대상 영역을 임의의 부영역으로 분할한 후, 공유면에서의 절점 연속성 등을 고려하지 않고 각 부영역을 독립적으로 유한요소 모델링할 수 있다. 컴퓨터 코드의 개발 및 수치 예제의 해석을 통하여 본 기법에 대한 기본적인 특성을 확인하였다.

• 한국조경학회지
• /
• 제32권3호
• /
• pp.91-105
• /
• 2004

Recently the function of a department store has changed to the concept of a multi-functional center because of the alternative stores such as discount stores, home shopping, and internet shopping. This means that the front plaza of a department store is not a personal or private space any more, but a public space. This study focuses on the special character of public space through the classification and preference types of department store front plazas. The major results of this study can be summarized as follows: (1) Components of front plaza of department store are classified by three factors. The first factor, named "space limit", has 14 elements ; the second, named "space decoration" has 16 elements ; third, named "activity", has 2 elements. The first preferred element is easily- used and easily- serviced wide space. The second preferred element is the equipment that is placed linearly along the street. The third preferred element is cultural events. (2) The comparison between the frequency and preference shows that the plazas could not satisfy the user-needs. (3) Preference factors of front plazas were examined to three characters such as familiarity, peculiarity, and openness. Familiarity, peculiarity, openness have a positive correlation in all types. Peculiarity especially influences the other two space - preference factors.

• 한국항공우주학회지
• /
• 제35권5호
• /
• pp.404-411
• /
• 2007

접촉 문제의 효율적인 유한요소 해석을 위하여 부영역, 공유면 및 접촉 공유면의 개념에 근거한 영역/경계 분할법을 제시하였다. 부영역과 공유면 또는 접촉 공유면을 결합하기 위한 등식 적합 조건을 벌칙 함수로 처리함으로써 모든 유효 강성 행렬이 양 정치화되므로, 역행렬과 같은 각종 행렬의 연산이 매우 간편해진다. 또한 전체 영역 형상이 복잡하더라도, 임의의 부영역으로 분할한 후 공유면에서의 절점 연속성을 고려하지 않고 각각의 부영역을 독립적으로 이산화할 수 있다. 간단한 수치 예제 해석을 통하여 본 기법의 기본적인 특성을 고찰하였다.

• Steel and Composite Structures
• /
• 제13권1호
• /
• pp.71-89
• /
• 2012

The paper investigates beam lateral buckling stability according to linear and non-linear models. Closed form solutions for single-symmetric cross sections are first derived according to a non-linear model considering flexural-torsional coupling and pre-buckling deformation effects. The closed form solutions are compared to a beam finite element developed in large torsion. Effects of pre-buckling deflection and gradient moment on beam stability are not well known in the literature. The strength of singly symmetric I-beams under gradient moments is particularly investigated. Beams with T and I cross-sections are considered in the study. It is concluded that pre-buckling deflections effects are important for I-section with large flanges and analytical solutions are possible. For beams with T-sections, lateral buckling resistance depends not only on pre-buckling deflection but also on cross section shape, load distribution and buckling modes. Effects of pre-buckling deflections are important only when the largest flange is under compressive stresses and positive gradient moments. For negative gradient moments, all available solutions fail and overestimate the beam strength. Numerical solutions are more powerful. Other load cases are investigated as the stability of continuous beams. Under arbitrary loads, all available solutions fail, and recourse to finite element simulation is more efficient.

• 한국전기전자재료학회논문지
• /
• 제23권5호
• /
• pp.408-412
• /
• 2010

The single-phase switched reluctance motor(SRM) generates the positive torque in the restricted section. So, it can not started by itself and the torque ripple is heavier than poly-phase. For self-starting and fixing rotating direction, the rotor should be placed at the rising inductance slope when stationary. The parking permanent magnet locates the rotor in the fixed position, which can be started by it-self. It is very simple and cost effective but has some drawbacks. It affects the rotor during the operation, so the characteristics of motor, such as a torque, speed, and ripple are changed to go bad. This paper presents the detent torque of parking magnet starting device through the finite element analysis and experiments. The finite element analysis is performed at incremental rotor positions over one detent torque cycle for any one pole. The prototype, fabricated in the previous research, is used for the experiments. The inductance, instant torque, and detent torque are calculated using the terminal voltage and phase current. Finally, the finite element analysis result and the experiment result are compared for analysis and validity.

• 한국전산구조공학회:학술대회논문집
• /
• 한국전산구조공학회 2006년도 정기 학술대회 논문집
• /
• pp.421-426
• /
• 2006

To examine the behaviors of traditional wooden structural frame in Korea in direction of beam, an experimental study was performed. The interior frame of Daewoongjeon of Bongjeongsa was selected as a model, which has two short exterior columns and one high inside column. The experimental frame has 1/2 scale and lateral forces are applied at high inside column by using drift control. The vertical gravity loads are applied on the frame. From the results of experiment it was shown that the stiffness and lateral capacity of the frame was increased when vertical loads are applied and the force-drift relationship in positive load direction was not same as in negative load direction. And push-over analysis are performed by using macro model in which the rotational and shear springs which were derived from the another experiments of subassemblies were used. The numerical analysis with macro model showed a good correspondence with the experiment within 2% story drift.

• 한국컴퓨터정보학회논문지
• /
• 제16권1호
• /
• pp.81-87
• /
• 2011

최근게임 산업에서 순기능적 역할로 이슈가 되고 있는 기능성 게임에 대하여 기술한다. 기능성 게임은 게임기술과 게임이론을 바탕으로 개발된 하드웨어 및 소프트웨어 애플리케이션으로 즐거움 외에 교육 및 특정 분야의 문제 해결 등의 다른 목적을 가지는 게임을 말한다. 이러한 기능성 게임은 게임적인 재미뿐만 아니라 교육적인 효과때문에 과학기술 분야 및 산업기술 분야의 교육 및 훈련에 유용하다. 본 논문에서는 기능성 게임에 대한 분류 및 동향에 대하여 기술하고, 장애인을 위한 디자인 고려 요소를 제안하고, 설문 조사를 통하여 그 결과를 입증하였다.