• 동의생리병리학회지
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• 제31권1호
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• pp.52-58
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• 2017

This study was aimed to examine relaxing effects of Acanthopanacis cortex(AC) through nitric oxide(NO) production and phosphodiesterase type 5(PDE-5) inhibition in corpus cavernosum. In order to define the relaxation effects of AC extract, rabbit corpus cavernous tissues were prepared in $2{\times}2{\times}8mm$ sized strip. AC extract ($0.01-3.0mg/m{\ell}$) were treated in contracted strips induced by phenylephrine(PE) and $N{\omega}$-nitro-L-arginine (L-NNA) was treated before AC extract-treated. And calcium chloride($Ca^{2+}$) 1 mM was infused into precontracted strips after pretreatment of AC extract in $Ca^{2+}-free$ krebs-ringer solution. When AC extract was applied to human umbilical vein endothelial cell(HUVEC), cell viability was measured by MTT assay, and NO concentration was measured by Griess reagent system. Ratio of smooth muscles to collagen fibers and eNOS, PDE-5 positive reaction were measured by histochemical and immunohistochemical process on mice corpus cavernosum. AC extract significantly affected relaxion of the cavernous strips, and the pretreatment of L-NNA inhibited AC extract-induced relaxation. Contraction induced by the addition of $Ca^{2+}$ was inhibited by treatment with the AC extract in $Ca^{2+}-free$ solution. In AC group, NO concentration, ratio of smooth muscle to collagen fibers, and eNOS positive reaction were increased, PDE-5 positive reaction was decreased compared to PE group. As a result of the above experiment, it was thought that AC extract inhibits the inflow of extracellular $Ca^{2+}$ by activating cGMP through the increase of eNOS / NO and the decrease of PDE-5 which inhibits cGMP activity, in the corpus cavernosum.

• 한국산학기술학회논문지
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• 제21권4호
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• pp.285-292
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• 2020

건설산업내 상생을 위한 정부의 중소기업 보호 정책에도 불구하고, 위축되어 가는 건설시장으로 인해 대·중·소기업간 수주양극화, 중소기업 및 지역기업의 수주 경쟁력 약화 및 수익성 약화 등의 문제는 여전히 개선되지 못하고 있는 실정이다. 미국 건설산업내 중소기업 및 지역기업에 대한 발주우대제도, 입찰우대제도, 지역기업 활용을 중심으로 건설산업 생태계 내 상대적 약자인 중소기업, 지역기업, 사회적약자소유기업 등을 위한 미국 연방 및 주정부의 정책 및 제도를 고찰하고, 이를 통해 바람직한 중·소기업 보호 및 경쟁력 강화 정책 방향 재고를 위한 국내 중소기업 보호 관련 정책에 시사하는 바를 도출하였다. 결론적으로 물량 배분을 기반으로 하는 국내 중소기업보호 관련 정책과는 달리 미국의 중소기업 및 지역기업 우대 제도는 해당 기업들을 일정비율이내에서 우대를 하되 공정경쟁 원리를 동시에 수용함으로써, 중소기업 및 지역기업 보호와 동시에 해당 기업들의 경쟁력을 강화 할 수 있는 방향에서 정책을 운용하고 있다. 따라서 향후 국내 중소기업 및 지역기업 보호 및 경쟁력 강화를 위해서는 일정부분 보호하는 동시에 중소기업의 경쟁력을 강화할 수 있는 방향으로 관련 제도를 정비할 필요가 있을 것으로 판단된다.

• 대한본초학회지
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• 제30권4호
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• pp.71-79
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• 2015

Objectives : These present study was designed to investigate the relaxation effects of Alpiniae Oxyphyllae Fructus(AOF) on isolated corpus cavernosum smooth muscle.Methods : Rabbit corpus cavernous tissues were prepared in strip. Then relaxation responses of AOF at 0.01-3 ㎎/㎖ in contracted strips induced by phenylephrine(PE) were measured. To evaluate mechanisms, indomethacin(IM) tetraethylammonium chloride(TEA), Nω-nitro-L-arginine(_L-NNA), methylene blue(MB) were treated before AOF extract(0.1-3 ㎎/㎖) infused into precontracted strips induced by PE. And 1 mM Ca²⁺was infused into precontracted strips after pretreatment of AOF extract(3 ㎎/㎖) in Ca²⁺-free krebs-ringer solution. NO concentration was measured by Griess reagent system. Ratio of smooth muscles to collagen fibers and eNOS positive reaction were measured by histocheminal and immunohistochemical process.Results : The cavernous strips were significantly relaxed by AOF extract 0.1, 0.3, 1, 3 ㎎/㎖ and the pretreatment with IM 10 μM,_L-NNA 100 μM, MB 10 μM inhibited relaxation of AOF compared to non-pretreatment, but the pretreatment with TEA 100 μM didn't affect relaxation of AOF. In a Ca²⁺-free solution, pretreatment with AOF reduced increase on contraction of strips by Ca²⁺supply than non-pretreatment. On HUVEC, NO concentration was increased. On corpus cavernosum of penis in Spontaneous Hypertensive Rat, ratio of smooth muscles to collagen fibers and eNOS positive reaction in AOF group were increased compared to PE groupConclusions : Taken this results, we can suggest that AOF extract exerts a relaxation effects on rabbit corpus cavernosum smooth muscle in part by suppressing influx of extracellular Ca²⁺throughout prostacyclin, the NO-cGMP system.

• 대한한방내과학회지
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• 제29권1호
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• pp.231-242
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• 2008

Objectives : We can find out the experimental reports of Yanggyuksanhwa-tang, which has the function of regulating blood pressure related with cerebral disease, and increasing local cerebral blood stream volume, also has the recoveries for the damage of vessel endothelium, and endothelium hypertrophy caused by angiospasm after subarachnoid hemorrhage, and reduces the contraction of smooth muscle, so simultaneously improves necrosis. The aim of this study is to investigate effect of Yanggyuksanhwa-tang protecting neuronal cells from being damaged by brain ischemia through using organotypic hippocampal slice cultures. Methods : We caused ischemic damage to organotypic hippocampal slice cultures by oxygen and glucose deprivation, and Yanggyuksanhwa-tang extract was added to cultures. Thereafter we measured area percentage of propidium iodide (PI)-stained neuronal cell, lactate dehydrogenase (LDH) levels in culture media and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)-positive cells. Results : Area percentage of PI-stained neuronal cells and count of TUNEL-positive cells in CA1 and DG area of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Yanggyuksanhwa-tang extract. LDH levels in culture media of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Yanggyuksanhwa-tang extract. Conclusions : Within pertinent density level, Yanggyuksanhwa-tang has cell protection effect that prevents brain ischemia damaging neuronal cells and apoptosis increasing.

• 대한정형도수물리치료학회지
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• 제20권1호
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• pp.1-7
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• 2014

Background: As the increase of forward head position, we studied the change of thickness of deep cervical flexor (DCF) compared of with sternocleidomastoid (SCM). we measured and analysed the change of thickness of the neck flexor for forward head position and cranio-cervical flexion exercise (CCFE). Methods: Using a cross-sectional design, we conducted the study selecting 35 healthy adults (12 males, 23 females). We measured the craniovertebral angle (CVA), instructed them to perform the CCFE, and measured the DCF and SCM using ultrasonography during the contraction and relaxation period. Results: Intra-reliability of SCM is .96, longus capitis is .92 and longus coli is .97. we compared according to the change of forward head position, Correlation of DCF is .841, and DCF/SCM is .754 by significant positive correlation. At the comparison of CCFE and Resting muscle thickness, SCM and DCF is .00, DCF/SCM is .68. Conclusion: There is a strong positive correlation between the change amount of DCF and DCF/SCM as the increase of CVA.

• 대한한방내과학회지
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• 제37권4호
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• pp.591-600
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• 2016

Objective: This study aimed to examine the relaxation effects and underlying mechanisms of Cynomorii herba (CH) extract in isolated rabbit corpus cavernous tissues.Methods: We experimented with CH extract (0.01-3.0 mg/mL). Nω-nitro-L-arginine (L-NNA) was experimented before the CH extract to contracted strips induced by phenylephrine (PE, 1 μM)and compared with nonexperimented. In addition, we experimented with calcium chloride (Ca²⁺, 1 mM) after pretreatment of the CH extract in Ca²⁺-free Krebs-Ringer solution to contracted strips induced by PE. The cell viability and nitric oxide (NO) concentration of human umbilical vein endothelial cells (HUVECs) were measured by an methylthiazol-2-yl-2, 5-diphenyl tetrazoliumbromide (MTT) assay and Griess reagent system. The ratio of smooth muscles to collagen fibers, in addition to eNOS- and PDE-5-positive reactions, was examined by histochemical and immunohistochemical staining.Results: The CH extract significantly induced the relaxation of the cavernous strips, and the pretreatment with L-NNA inhibited CH extract-induced relaxation. The L-NNA pretreatment reduced the increased contraction induced by the addition of Ca²⁺in Ca²⁺-free solution. Furthermore, the NO concentration of the HUVECs increased. When the CH extract was applied to the corpus cavernosum of the penis (CCP) of Sprague Dawley rats, the ratio of smooth muscles to collagen fibers by PE and the formation of eNOS around the helicine artery increased. However, the CH extract treatment decreased PDE-5 positive reactions.Conclusions: These results show that the relaxation effects induced by the CH extract are associated with the suppression of the influx of extracellular Ca²⁺ via the production of NO and eNOS and inhibition of PDE-5.

• 대한약리학회지
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• 제22권1호
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• pp.34-44
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• 1986

본 실험실에서는 한국 특산인 매자나무 (Berberis koreana Palibin)의 뿌리에서 majarine(isoquinoline alkaloid)을 분리하여 여러 가지의 약리작용을 검토한 바 있다. 현재는 majarine의 화학적 구조를 변화시켜 그 유도체의 약리작용을 검색하고 있으며, 그 중 majarine의 isoquinoline ring에 치환되어 있는 두 methoxy group 중 하나를 hydroxy기로 치환된 majarol을 합성하였다. Majarol을 흰쥐 정맥내로 투여하여 혈압 및 심박동수의 변화와 epinephrine, phentolamine, isoproterenol, propranolol, atropine, diphenhydramine과 hexamethonium 등에 대한 약물 상호작용을 관찰하였으며 또 적출 개구리 심장에 대한 작용을 관찰하여 다음과 같은 성적을 얻었다. Majarine을 저용량(0.5-2 mg/kg)에서 용량의존적으로 현저하고 일시적인 혈압 강하와 약간의 심박동수 감소를 나타냈으며, 고용량(>5mg/kg)에서는 이차적으로 지속적인 혈압강하와 심박동수 감소를 나타냈다. 일시적인 혈압강하작용은 atropine sulfate, diphenhydramine과 hexamethonium에 의해 억제되지 않았고, epinephrine의 혈압 상승작용은 majarol의 전처치로 현저하게 차단되었으며, majarol 투여시 나타나는 지속적인 혈압 강하는 심박동수 감소에 수반되어 나타났고 이는 propranolol에 의해 억제되지 않았으며, isoproterenol의 positive chronotropic effect는 majarol 전처치로 차단되지 않았다. majarol은 저용량에서 적출개구리 심장의 수축기 장력을 증가시켰으며 고용량에서는 개구리 적출심장에 대해 억제적으로 작용하였다. 이러한 결과로 보아 majarol의 일시적이고 현저한 혈압강하작용은 ${\alpha}$-adrenoceptor 차단작용에 의한 것으로 사료되고, 이차적으로 나타나는 지속적인 혈압강하작용은 심박동수의 감소와 동시에 나타나는 것으로 보아 심장에 대한 작용인 것으로 추정되며, majarol의 심장에 대한 작용을 ${\beta}$-adrenoceptor에 대한 작용보다는 심근에 대한 직접적인 작용인 것으로 추정된다.

• 대한물리의학회지
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• 제8권4호
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• pp.593-600
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• 2013

PURPOSE: We investigated the relationship between personality, music type and MVIC, power for the knee extensor on inducing muscle fatigue. METHODS: The subjects of this study were 22 healthy students. MVIC(Maximum Voluntary Isometric Contraction) and power in knee extension was measured by PRIMUS RS. Personality types was measured by MBTI(Myers-Briggs Type Indicator) that is an instrument designed to provide descriptive profiles of personality types and preferences. The collected data were analyzed using the independent t-test, MANOVA and Pearson-test. RESULTS: There was no significant differences both MVIC and power of the knee extensor according to personality and music type. The power was significantly increased by increasing the MVIC during a knee extension on inducing muscle fatigue without music type(r= .786~.896). There was no significant correlation both MVIC and power of the knee extensor according to personality. CONCLUSION: There was a positive correlation between power and MVIC during a knee extension on inducing muscle fatigue without music type. There was no significant correlation both MVIC and power of the knee extensor according to personality.

• 한국포장학회지
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• 제10권1호
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• pp.55-62
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• 2004

Synthesis of poly[N-(formyloxyphenyl)maleimide](PFOMI) as photopolymer were investigated with various kinds of photosensitive groups. Generally, photopolyimide have some deficiencies in solubility, sensitivity, reserve stability of the photosensitive solution, and the precision of image pattern. The study has been required on those polymers which have high glass transition temperature and photo efficiency, and low dielectricity. The existing condensation resins require high curing temperature and perfect elimination of subreacted materials that are produced during the process after irradiation and various membrane damages such as the deformation and contraction in image pattern cure. In this study poly[N-(hydroxyphenyl)maleimide](PHPMI) was synthesized. The PHPMI were analyzed by H-NMR and FT-IR. The measured number average molecular weight of PHPMI was produced was $1.06{\times}10^4$. Poly[N-(formyloxyphenyl)maleimide](PFOMI) as a type of photo-Fries rearrangement was synthesized by NHPMI and formic acid followed by radical polymerization. PFOMI was analyzed by FT-IR, and photocharacteristics was investgated by UV spectra and FT-IR before and after UV irradiation. Based on the image characteristics of PFOMI measured from optical micrographs, it was formed that the resolution of positive type PFOMI was $0.5{\mu}m$.

• 대한임상전기생리학회지
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• 제9권2호
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• pp.7-12
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• 2011

Purpose : We studied the correlation between reference voluntary contraction (% RVC) of vastus lateralis (VL) and vastus medialis (VM), Berg balance scale (BBS), and timed-up & go (TUG) test. Methods : We recruited 30 stroke patients from a rehabilitation center at a hospital.? All subjects could walk with or without an assisting device. Subjects were evaluated with % RVC of VL and VM, BBS, and TUG. The data were analyzed using a Pearson correlation coefficient. Results : The % RVC of VL and VM and BBS (p<0.01) showed a significant positive correlation. TUG negatively correlated with % RVC of VL and VM and BBS (p<0.01). Conclusion : Lower extremity muscle activity increases balance and walking ability. We recommend the implementation of lower extremity strength exercises in the rehabilitation of stroke patient.