• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.2
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• pp.275-280
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• 1998

The possibility of utilizing Porphyra yezoensis, Undaria pinnatifida and Laminaria religionsa into algae-tea having biological functionality was investigated by analysis of functional component, experimentation for low viscosity and various pretreatments. In water soluble fraction of powdered algae extracted for 3 minutes with 30 times of hot wate, major functinal components were composed of 1.53% porphyran and 170.04mg% taurine in P. yezoensis, 1.09% fucoidan and 1.18% sodium sodium alginate in U. pinnatifida, and 1.28% fucoidan, 1.99% soidum laginate and 371.25mg% iodine in L.religiosa, on dry basis. For lowering viscosity and masking off-flavor in each water soluble fraction, it was desirable that P. yezoensis was washed for 12 hours in sea water and 30 mimutes in fresh water, dried at 3 to 5$^{\circ}C$, powdered to size of 30 mesh nd then roasted for 3 minutes at 12$0^{\circ}C$, and that both U. pinnatifida and L. religiosa were washed, semidried to 40~50% moisture content, heated for 40 minutes at 12$0^{\circ}C$ by autoclave, dried, powdered to size of 30 mesh and then roasted for 5 minutes at 11$0^{\circ}C$.

• Journal of fish pathology
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• v.26 no.3
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• pp.275-282
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• 2013

The use of activating treatment agent (formerly acid treatment agent) has been an effective strategy to remove deleterious epibiont organisms such as diatoms and green seaweeds, and it has greatly contributed to increase in Porphyra production. Although many manufacturers supply many kinds of activating treatment agent with different components in these days, no report about their effects on Porphyra culture was found. In this paper, effects of a commercial activating treatment agent were evaluated for practical use in Porphyra culture. No difference was found in dead cell ratios(%) of Porphyra yezoensis thalli between treated and control groups. However, dead cell ratios of Monostroma nitidum thalli were increased from 0~4.6% to 99.0~100% after the treatment. Bathing Porphyra thalli in activating treatment agent resulted in a great decrease in epiphytic bacterial number attached to the thalli from $10^2{\sim}10^{11}$ cells/g to $0{\sim}10^5$ cells/g but did not change the colour of the thalli. These results suggest that bathing Porphyra thalli in activating treatment agent could be a promising strategy to remove green algae, diatoms and bacteria.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.16 no.4
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• pp.335-340
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• 1983

The effects of water temperature, salinity, and silt and clay on the photosynthetic activity of Porphyra yezoensis were measured. The rate of photosynthesis of P. yezoensis increases as the water temperature rises in the range of $8{\sim}16^{\circ}C$ and begin to decrease at $18^{\circ}C$. In the salinity range of $21.5{\sim}33.5\%0$, the rate of photosythesis of P. yezoensis was increased in the sea water of $29.5\%0$ salinity and decreased in $21.5\%0$ salinity. The rates of photosynthesis of P. yezoensis were significantly decreased with increase of the concentration of silt and clay and the time of exposure to suspended silt and clay. Of the combined effects of salinity, and silt and clay on the photosynthetic activity of P. yezoensis, the effects of silt and clay were higher in the low salinity of $21.5\%0$ and $33.5\%0$. The wet weight of P. yezoensis showed a remarkable loss with increase of the concentration of silt and clay and the time of exposure to silt and clay.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.6
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• pp.868-877
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• 2013

To understand the effects of marine environmental and meteorological parameters on laver Porphyra yezoensis production at Nakdong River Estuary, we analyzed marine environmental (water temperature, salinity, nutrients, etc.) and meteorological properties (air temperature, wind speed, precipitation, sunshine hours) with yearly and monthly variations in laver production over 10 years (2003-2013). Air and water temperature, wind speed, sunshine hours and precipitation were major factors affecting yearly variability in laver production at the Nakdong River Estuary. Lower air and water temperatures together with higher levels of nutrients and sunshine and stronger wind speeds resulted in higher laver harvests. Salinity and nitrogen did not show clear correlations with laver production, mainly due to the plentiful supply of nitrogen from river discharge and the low frequency of environmental measurements, which resulted in low statistical confidence. However, environmental factors affecting monthly laver production were related to the life cycle (culturing stage) of Porphyra yezoensis and were somewhat different from factors affecting annual laver production. In November, a young laver needs lower water temperatures for rapid growth, while a mature laver needs much stronger winds and more sunshine, as well as lower temperatures for massive production and effective photosynthesis, mostly in December and January. However, in spring (March), more stable environments with fewer fluctuations in air temperature are needed to sustain the production of newly deployed culture-nets ($2^{nd}$ time culture). These results indicate that rapid changes in weather and marine environments caused by global climate change will negatively affect laver production and, thus, to sustain the yield of and predict future variability in laver production at the Nakdong River estuary, environmental variation around laver culturing farms needs to be monitored with high resolution in space and time.

• KSBB Journal
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• v.14 no.6
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• pp.702-706
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• 1999

The genetic responses of aquaculturable seaweed Prophyra yezoensis tissue by acid shock have been compared using differential display technique. The tissue has been challenged in seawater containing 0.05% hydrogen chloride(pH 3.0) for 5 min, then rehabilitated in normal seawater for 10 min, 30 min, 60 min and 4 hrs, respectively. Total RNA was extracted by LiCl-guanidinium method. The cDNA was synthesized by reverse transcription with random hexamers and amplified by PCR with arbitrary primers. The genetic fragment disappeared by acid shock was selectively isolated from agarose gel and sequenced with a DNA auto sequencer. One of the acid-labile gene(605 bp) was identified as a dethiobiotin synthetase gene according to sequence alignment analysis by the NCBI BLAST search program.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 1996.06a
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• pp.230-232
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• 1996

• Proceedings of the Korean Nutrition Society Conference
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• 2001.05a
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• pp.275.1-275
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• 2001

• ALGAE
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• v.20 no.2
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• pp.121-125
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• 2005

The modern integrated fish-seaweed mariculture has been tested to reduce the environmental impacts of an intensive fed culture. To obtain the best seaweed bioremediation performance, the effects of therapeutants used for fish disease control on the selected seaweed species should be considered. As a selected seaweed, Porphyra yezoensis was tested with six commercial antibiotics including erythromycin thiocyanate_A, erythromycin thiocyanate_B, oxytetracycline, doxycycline, pefloxacin, and amoxicillin trihydrate under the batch incubation at a photon flux density of 10 $\mu$mol ${\cdot}m^{-2}\;{\cdot}\;s^{-1}$ at 15$^{\circ}C$. Among the tested commercial antibiotics, erythromycin thiocyanate_A, erythromycin thiocyanate_B, oxytetracycline, and doxycycline showed decreases in Fv/Fm, the photochemical efficiency of photosystem II, with a dose-dependant and time-dependant manner. From the quenching analysis of chlorophyll fluorescence, three differential patterns were observed in the antibiotics-treated Porphyra: (1) high nonphotochemical quenching (NPQ) and low photochemical quenching (qP) in the cases of Erythromycin thiocyanate_B and amoxicillin trihydrate, (2) high NPQ and high qP in the case of pefloxacin and (3) low NPQ and low qP in the case of oxytetracycline. These results indicated that antibiotics affected in various ways on the photosynthetic apparatus, reflecting differential lesion sites of antibiotics. In addition, the rates of ammonium uptake also decreased with a decrease of Fv/Fm in P. yezoensis thalli treated with erythromycin thiocyanate_B and oxytetracycline. Therefore, the four antibiotics mentioned could affect the bioremediation capacity of the selected seaweed species in the integrated fish-seaweed mariculture system due to the decrease of photosynthetic activity and the simultaneous decrease of ammonium uptake.

• Korean Journal of Environmental Biology
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• v.32 no.4
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• pp.297-305
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• 2014

In order to understand the contribution of seaweed aquaculture to nutrients and carbon cycles in coastal environments, we measured the nutrients & carbon uptake rates of Porphyra yezoensis Ueda sampled at Nakdong-River Estuary using a chamber incubation method from November 2011 to April 2012. It was observed that the production rate of dissolved oxygen by P. yezoensis (n=30~40) was about $68.8{\pm}46.0{\mu}mol\;{g_{FW}}^{-1}h^{-1}$ and uptake rate of nitrate, phosphate and dissolved inorganic carbon (DIC) was found to be $2.5{\pm}1.8{\mu}mol\;{g_{FW}}^{-1}h^{-1}$, $0.18{\pm}0.11{\mu}mol\;{g_{FW}}^{-1}h^{-1}$ and $87.1{\pm}57.3{\mu}mol\;{g_{FW}}^{-1}h^{-1}$, respectively. There was a positive linear correlation existed between the production rate of dissolved oxygen and the consumption rates of nitrate, phosphate and DIC, respectively, suggesting that these factors may serve as good indicators of P. yezoensis photosynthesis. Further, there was a negative logarithmic relationship between fresh weight of thallus and uptake rates of nutrients and $CO_2$, which suggested that younger specimens (0.1~0.3 g) were much more efficient at nutrients and $CO_2$ uptake than old specimens. It means that the early culturing stage than harvesting season might have more possibilities to be developed chlorosis by high rates of nitrogen uptake. However, N & C demanding rates of Busan and Jeollabuk-do, calculated by monthly mass production and culturing area, were much higher than those of Jeollanam-do, the highest harvesting area in Korea. Chlorosis events at Jeollabuk-do recently might have developed by the reason that heavily culture in narrow area and insufficient nutrients in maximum yield season (Dec.~Jan.) due mostly to shortage of land discharge and weak water circulation. The annual DIC uptake by P. yezoensis in Nakdong-River Estuary was estimated about $5.6{\times}10^3\;CO_2$ ton, which was about 0.03% of annual carbon dioxide emission of Busan City. Taken together, we suggest more research would be helpful to gain deep insight to evaluate the roles of seaweed aquaculture to the coastal nutrients cycles and global carbon cycle.

• Journal of the Korea Convergence Society
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• v.9 no.12
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• pp.81-88
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• 2018

Human gingival fibroblast (HGF) is the main cell type existed in periodontium and produces a variety of inflammatory mediators by external stimuli. In this study, the anti-inflammatory activity of Porphyra yezoensis ethanol extract (PYEE) on LPS-PG lipopolysaccharide from Porphyromonas gingivalis activated HGF-1 cell. Up-regulated iNOS and COX-2 expressions by LPS-PG were significantly attenuated by PYEE treatment in a dose-dependent manner. In addition, activated nuclear factor $(NF)-{\kappa}B$ was also dose-dependently inhibited by PYEE treatment. Among upstream signaling molecules, PYEE treatment inhibited phosphorylation of c-Jun $NH_2$-terminal kinase (JNK) but did not give any effect on other molecules. On the other hand, one of phase II enzymes, NAD(P)H:quinone dehydrogenase (NQO)-1, was analyzed due to its anti-inflammatory activity, which was upregulated by PYEE treatment. Consequently, PYEE could be candidates for the prevention and treatment of periodontal diseases.