• The Korea Genome Conference
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• 2004.07a
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• pp.44.2-44.2
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• 2004

• The Korean Journal of Quaternary Research
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• v.17 no.2
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• pp.39-41
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• 2003

Pollen and spores as well as their morphological characteristics are biological stability materials in which contains historical information about vegetation and climate changes. As noted researcher Neishtadt in 1971, the lake and swamp deposits were developed only in Holocene period. The geological history of Darkhad depression which is situated in the Northern end of Mongolia from ancient time become under scientific interest of many researchers. Our investigation was focused on pollen analysis of lake sediment of Darkhad depression which caused bottom of paleolake. In Mongolia the palynology science is beginning to develop since 1990 and in 2000 the laboratory of palynology was established at the department of Biology, State Pedagogical University of Mongolia. Researchers from this laboratory working on pollen morphology of present flora in order to classify plants taxonomy. Another part of our investigation is mellitopalynology, which is studying of pollen grains in a honey. Although, this research has been extending by pollen analysis of sediment and lake deposits in past few years on the basis of high sensitive microscope and modem technology.

• Genomics & Informatics
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• v.3 no.3
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• pp.53-62
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• 2005

MicroRNAs play an important role in regulating gene expression, but their target identification is a difficult task due to their short length and imperfect complementarity. Burge and coworkers developed a program called TargetScan that allowed imperfect complementarity and established a procedure favoring targets with multiple binding sites conserved in multiple organisms. We improved their algorithm in two major aspects - (i) using well-defined UTR (untranslated region) database, (ii) examining the extent of conservation inside the 3' UTR specifically. Average length in our UTR database, based on the ECgene annotation, is more than twice longer than the Ensembl. Then, TargetScan was used to identify putative binding sites. The extent of conservation varies significantly inside the 3' UTR. We used the 'tight' tracks in the UCSC genome browser to select the conserved binding sites in multiple species. By combining the longer 3' UTR data, TargetScan, and tightly conserved blocks of genomic DNA, we identified 107 putative target genes with multiple binding sites conserved in multiple species, of which 85 putative targets are novel.

• The Korean Journal of Zoology
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• v.36 no.2
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• pp.304-318
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• 1993

1991년 8월부터 그이듬해 7월까지 채취된 무지개 송어(Oncorhvnchus mykiu) 난모세포의발달 단계를 이해하기 위하여 광필 및 전자현미경을 이용하여 본 각구를 실시하였다. 건국 대학교 축산대학 양수.장에서 수온이 14-16$^{\circ}C$이고 용존산소량이 7.6 $\pm$ 0.3ppm. PH 5.8 $\pm$ 0.2인 자연채광 상태인 1개의 10m3 탱크에서 N R.C. 사양표준에 준한 펠렛트 배합사료를 급여하여 연중 사육중인 체중 700-1,2009의 한복새끼인 무지개 송어 암컷 120마리를 공시희.로 이용 하였다. LPO(late perinucleolus oocyte), EMO(early maturing oocyte) 단계에서 과립막 세포와 협막세포와 같은 난포막 세포는 단층구조로 부터 입 방형구조로 변화되었고. 완전한 성장, 성숙 및 과숙기에 방사대(Eons rsdiat3)에 있는 pore canals의 입구가 봉쇄되었다. 방사대는 맨바깥목에 균일하면서 점은 두께의 막과 나선형의 두꺼운 복의 2개의 막으로 구성되어 있으며. 성숙기에 있는 난모세포에는 수초 및 자갈에 붙을 수 있는 온수성 어종 보다 상대적으로 얇은 점액성의 막이 그 주위를 둘러싸고 있다. 난모세포가 성장함에 따라 세포질 주변부 및 임립등 세포내에 막대기 형태의 미토콘드리아와 비대해진 내형질세강의 육가 급증하였다. 전자밀도가 높은 소포는 방사대에 인접한 난세포질 주변부에 많이 존재하며. 이는 성장중인 방사대의 pore Canals을 통해서 간으로 부터 합성된 단백질과 탄수화물을 수송하는 것과 밀접한 관계가 있는 것으로 사료된다. 자연적인 조건하에서 fP포막은 난형성 및 이에 영향을 미치는 성호르몬의 발달에 충분한 대르몬을 계절에 따라 분비시키는 데에 커다란 영향을 미치는 것으로 나타났다.

• Genomics & Informatics
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• v.17 no.3
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• pp.26.1-26.12
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• 2019

Identification of fusion gene is of prominent importance in cancer research field because of their potential as carcinogenic drivers. RNA sequencing (RNA-Seq) data have been the most useful source for identification of fusion transcripts. Although a number of algorithms have been developed thus far, most programs produce too many false-positives, thus making experimental confirmation almost impossible. We still lack a reliable program that achieves high precision with reasonable recall rate. Here, we present FusionScan, a highly optimized tool for predicting fusion transcripts from RNA-Seq data. We specifically search for split reads composed of intact exons at the fusion boundaries. Using 269 known fusion cases as the reference, we have implemented various mapping and filtering strategies to remove false-positives without discarding genuine fusions. In the performance test using three cell line datasets with validated fusion cases (NCI-H660, K562, and MCF-7), FusionScan outperformed other existing programs by a considerable margin, achieving the precision and recall rates of 60% and 79%, respectively. Simulation test also demonstrated that FusionScan recovered most of true positives without producing an overwhelming number of false-positives regardless of sequencing depth and read length. The computation time was comparable to other leading tools. We also provide several curative means to help users investigate the details of fusion candidates easily. We believe that FusionScan would be a reliable, efficient and convenient program for detecting fusion transcripts that meet the requirements in the clinical and experimental community. FusionScan is freely available at http://fusionscan.ewha.ac.kr/.