• Horticultural Science & Technology
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• v.19 no.3
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• pp.373-377
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• 2001

This study was conducted to find optimum pollination methods for overcoming pre-fertilization incompatibility in interspecific crosses between L. longiflorum 'Gelria' (LG) and L. cernuum (LC). In cross combination of $LC{\times}LG$, the survival rate of the ovaries was higher in the cut style pollinations than in normal stigmatic pollination. Among the various cut style pollination methods, pollination after cutting the styles 2 3mm length above the ovary and top of the ovary were the most effective ones. No ovaries and ovules survived in cross pollination of $LC{\times}LG$. In self-pollination of LG and cross pollination of $LC{\times}LG$, pollens germinated on the stigma by 12 hours and reached to the ovules by 90 hours after pollination. However, in the cross pollination of $LC{\times}LG$, pollen tubes elongated very slowly until 48 hours after pollination and a lot of deformed tubes were observed in the styler canal having swelled tube tip and irregular heavy callose deposition. The pollination time after anthesis also affected on the survival rate of ovaries and ovules showing the best result when pollinated on three days after anthesis. Stigma exudate promoted slightly the enlargement of ovaries, but the survival rate of ovaries and ovules was not increased.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.1
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• pp.15-18
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• 1998

Receptivity of corn silk to pollen decreases with time. For effective pollination, it is important to study the receptivity of corn silks in relation to time elapsed after their emergence. Two commercial corn hybrids, 'Kwanganog' and 'Suwon 19', were tested for their silk growth and effects of delayed pollination on kernel development for 1 to 13 days after emergence of the first silk. Silks elongated rapidly for the first 3 days and then gradually decreased. Silks grew more than 30mm per day for the first 3 days and stopped growing on the 11 or 12th day after emergence of the first silk. Filled ear length decreased slowly for the first 8 days, and then decreased rapidly. Similar trends were observed for number of kernel rows and number of kernels per row or ear. The highest numbers of kernels per row or ear were observed when plants were pollinated 2-4 days after silking. These numbers decreased gradually up to 8 days, and then decreased sharply. This result indicates that 8 day-delayed pollination has no influence on kernel development. There were negative correlations between silk length and ear characteristics except kernel weight. Silk growth rate was positively correlated with filled ear length, row number, and kernel number. It might be assumed that delayed pollination by one week after the first silk emerged would not affect on kernel set.

• Journal of Korean Society of Forest Science
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• v.98 no.6
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• pp.696-702
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• 2009

Yellow poplar (Liriodendron tulipifera L.) is an insect-pollinated tree species with large, perfect flower, and its seed sets average only about 10 percent naturally. In its controlled pollination, pollination bags are usually taken to prevent unwanted pollination, but bagging is an expensive and time-consuming process. Therefore, this study was conducted to determine the need of pollination bag by estimating how much unintended pollination would occur when different cross methods were applied. Five different pollination methods were applied as follows: 1) natural open pollination (i.e. insect pollination) as a reference, 2) self-pollination; no removing reproductive organs with bagging, 3) open pollination; emasculated(removing sepal, petal and stamen) without bagging, 4) controlled pollination; emasculated with bagging and 5) controlled pollination; emasculated without bagging. Very low value of full seed rate (0.2%) was observed in method 3, it was suggested that removing stamen and petal restrict the activity of pollen vectors like bee. Difference in the full seed rate between method 4 and method 5 was not significant (27.9% versus 24.0%, respectively). Consequently, controlled pollination without bagging might be an alternative method for extensive breeding and mass production of seeds in yellow poplar.

• Journal of Bio-Environment Control
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• v.23 no.3
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• pp.256-261
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• 2014

This study was carried out to determine the effect of the artificial pollination on fruit set, seed formation and fruit quality in kiwifruit and to confirm the effective pollination time for optimal fruit set in the shelter greenhouse cultivation. Also, the effect of artificial pollination practice by the pollen diluent solution was tested. By artificial pollination, fruit set, seed formation, and fruit quality was improved both in the open field condition and in the shelter greenhouse cultivation. In order to find the effective pollination period (EPP), artificial pollination was carried out every day until 7 days after full bloom. Number of seeds and fruit set rate and fruit growth of kiwifruit were no significantly different until 4 days after full bloom (DAFB), but, those of kiwifruit from 5 DAFB significantly decreased. Consequently, the effective pollination period of kiwifruit in the shelter greenhouse was 4 days after full bloom. Also, artificial pollination by the pollen diluent solution was showed the similar fruit set and fruit quality compare to that by lycopodium powder. Accordingly, artificial pollination by the pollen diluent solution should be very effective practice for labor saving in kiwifruit cultivation.

• Journal of Plant Biology
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• v.27 no.3
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• pp.107-127
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• 1984

An anthecological study of Tilia in Korea was undertaken for six native and one introduced species. Flowers are protoandrous with anther and stigma opening during the day-time in section(sect) Anastraea and the night-time in sect Astrophilyra. Nectar production was coincided with anther and stigma opening. It is revealed that the primary role of floral bracts was attracting pollinators especially in sect Astrophilyra. The peak of stamen and stigma function, and the production of nectar and odor were closely related with pollinator attraction. Forthy-three species of insects in 30 families were identified for the pollination studies; bees were the most commonly observed visitors in sect Anastraea, while moths were the primary visitors in sect Astrophilyra. Pollination study indicate that it is section specific. But within a section, pollination is not species specific thus interspecific hybridization occurs.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.171-175
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• 2000

This study was carried out to obtain the basic informations on the flowering habit, pollination patterns and seed setting in Astragalus membranaceus. The first flowering appeared at the 20-22 node on main stem and there is the indeterminate inflorescence that the flowering progress developed toward the tip of the main stem and also toward the tips of branches. According to the flowering time, the number of peduncles were increased in early of Aug. Then, the number of bearing pods and that of seed setting were increased at that time. The length of peduncles with small flower was 6.6-7.3cm, and the number of small flowers were 13-17 per peduncles. The percentage of seed setting was 30-45% per peduncles and seeds were 4.8-5.3 per pod in peduncles. On 30 days after pollination, the pod was developed with the length about 3.8cm and the width about 8mm and the seeds were 7.3per pod. According to pollination treatment, wrapping sotted no seeds and the artificial pollination sotted seeds . about 5% in late of jul. to late of Aug. but increased about 13% of seed set after early of Sep. in A. membranaceus.

• Journal of Apiculture
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• v.33 no.4
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• pp.239-250
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• 2018

We investigated the pollination activity of honeybees (Apis mellifera L.) in terms of different climatic conditions in the greenhouse cultivation of watermelons (Citrullus lanatus L.) during winter. The aim of the study was to search a climatic condition which effectively can be use honeybees as pollinators during the flowering season of watermelons in winter or early spring. The average climatic conditions inside the greenhouse during the bee activity time (BAT)-between 10:00 and 16:00 in mid-Februarywere a temperature of $30.4^{\circ}C$, relative humidity of 53.7%, illuminance level of 22,728.4lx, and UV level of $0.233mW/cm^2$. Bee traffic and foraging activity were at their greatest at 10:00 and tended to decrease with time. Male watermelon flowers typically dehisced between 10:00 and 12:00. Climatic conditions were significantly correlated with bee activities, including bee traffic and foraging activity. Bee activities were positively correlated with temperature, illuminance level, and UV level but negatively correlated with relative humidity. Temperature had the greatest effect on honeybee behavior. Among the foraging honeybees, the number of high-flying bees that did not pollinate flowers showed a strong positive correlation with temperature, and the number of bees landing on the flowers showed a positive correlation with the UV level. The temperature range inside greenhouses at which the pollination activities of honeybees can be maintained efficiently during winter watermelon pollination was found to be $21{\sim}25^{\circ}C$.

• Korean Journal of Medicinal Crop Science
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• v.2 no.1
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• pp.81-85
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• 1994

This experiment was carried out to investigate the fertilization and the size of mature female and male gametophytic parts of Pulsatilla koreana after artificial pollination. The size of pollen is $26.5{\mu}m$ at the time of anther dehiscence, that is, about $3{\sim}4$ days after pollination. Synergid nucleus, egg nucleus, and polar nucleus are 10.0, 15.0, and $32.5{\mu}m$ respectively at the time of completing egg apparatus formation, that is, about 2 days after pollination. Poller tubes germinate on stigma about 10 hours, passing lower part of style about 30 hours, penetrating into micropyle about 35 hours after pollination. Sperm nucleus penetrates into polar nucleus about 40 hours and egg cell about 48 hours after pollination. But, there seems to be different among the individuals. Multinuclei and multinucleoli are formed in egg cell, synergid, and polar nucleus about the time of fertilization. Proembryo is formed about 4 days, being changed to large globular form about $6{\sim}8$ days after pollination. Endosperm nuclei divide into free nuclei after fertilization and change to cotyledon in gymnosperm. There seems to be same phenomena in Pulsatilla koreana.

• Journal of Species Research
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• v.4 no.2
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• pp.145-151
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• 2015

Morphological changes of flowers and insect visitors were observed to investigate pollinator of Caesalpinia decapetala. The flowers of C. decapetala are protandrous. Functionally, the flower is changed from male to female. As a male, pollen grain is released after anther dehisced while style is immature. After completed pollen grain release, the style starts to lengthen. It helps the stigma to easily touch the carpenter bee's thorax covered with pollen grain. At this time, flower functions as a female. The majority of taxa and individuals observed were Hymenoptera. The most frequent visitor was the Xylocopa appendiculata circumvolans, carpenter bee. Carpenter bees exhibited only typical pollinator behavior among flower visitors, with touching reproductive organs and seeking nectar at the same time. The pollination behavior is as follows. Soon after carpenter bees perceived guide mark, they foraged rightward and grasped style and stamens with legs and they inserted proboscis into standard petal to seek nectar. With this behavior, the pollen grains of the male flower transfer to the ventral thorax of the carpenter bee. As the carpenter bee moves to another female flower, the deposited pollen grains are delivered to the stigma.

• Korean journal of applied entomology
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• v.46 no.2
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• pp.287-293
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• 2007

The pollinating activity of bumblebee, Bombus ignitus was investigated at the blossom of Peach [Prunus persica (L.) Batsch] planted in vinyl house. The major time of foraging activity by B. ignitus was between 08:00 and 7:00, and the peak time of pollinating activity by B. ignitus was 11 :00 in the peach vinyl house from February 28 to March 5 in 2002. The rate of fruit set and the rate of marketable fruit by foraging activity of B. ignitus were much better than artificial pollination, and the effect of labor reduction by the pollinating activities of B. ignitus released against th ε artificial pollination was took remarkable profits over 93%. Therefore, the foraging activity of B. ignitus in peach vinyl house brought satisfactory results among the rate of fruit set, the rate of marketable fruit and the effect of labor reduction against the artificial pollination.