• 제목/요약/키워드: pollen source

검색결과 27건 처리시간 0.024초

Occurrence of an invertase producing strain of Aspergillus niger LP5 isolated from longan pollen and its application in longan syrup production to feed honey bees (Apis mellifera L.)

  • Danmek, Khanchai;Ruenwai, Rawisara;Sorachakula, Choke;Jung, Chuleui;Chuttong, Bajaree
    • Journal of Ecology and Environment
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    • 제46권2호
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    • pp.136-143
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    • 2022
  • Background: In northern Thailand, the longan flower is the principal nectar source for honey production. Microorganisms play a critical function in the agricultural ecology. The morphological characteristics of fungal species found in longan pollen were studied. Aspergillus spp. were found to be invertase-producing strains and were employed in the longan syrup production process. The purpose of this study was to evaluate the effects of invertase-added longan syrup on the adult honey bee population numbers that were fed by this syrup for 16 weeks. Results: Different fungal species were found in longan pollen samples. Aspergillus was the main genus, with three predominant sections: Nigri, Flavi, and Terrei. Other isolated species were Trichoderma spp., Rhizopus spp., Neurospora spp., Chaetomium spp., Fusarium spp. and Penicillium spp. However, Aspergillus spp. is the only fungal species that produces the enzyme invertase. The invertase-producing strains belonging to the Aspergillus section Nigri were found to be A. niger LP5 with an optimum activity at pH 6.0 and 60℃. When A. niger LP5 invertase was used for longan syrup processing, the highest levels of glucose (3.45%) and fructose (2.08%) were found in invertase added longan syrup (C), while fresh (A) and boiled longan syrup (B) had lower contents of both sugars. The sucrose content was detected in (A) at 4.25%, while (B) and (C) were at 4.02% and 3.08%, respectively. An appropriate amount of sugar to feed and maintain the honey bee population was considered. The data showed no statistically significant differences between the two selected forms of longan syrup compared to the sugar syrup examined by the adult honey bee population. Conclusions: The main species of isolated fungi from longan pollen were Aspergillus spp. The discovery of an invertase-producing strain of A. niger LP5 has enabled its application for enzyme utilization in the invert sugar preparation process. The adult worker bee populations fed by longan syrup from both boiled and invertase-added sources showed an increasing trend. Artificial syrup made from longan fruit to feed honey bees when natural food sources are limited can be applied.

Effects of several factors on pollen germination in Platycodon grandiflorum

  • Kwon, Soo Jeong;Lee, Ui Gun;Moon, Young Ja;Cho, Gab Yeon;Woo, Sun Hee;Boo, Hee Ock;Koo, Jin-Woog;Kim, Hag Hyun
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.172-172
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    • 2017
  • Pollen germination and its' viability in bellflower hybrid system are of great importance. The present study was conducted to investigate the several factors underlying the pollen germination of Platycodon grandiflorum and obtain the basic data for effective artificial pollination for the production of sound specifies. The pollens of Platycodon grandiflorum started germination from one hour after planting, and the germination was actively progressed as time dependent manner. For lighting conditions, the germination of pollens under the light was faster by more than twice than that without the light. Furthermore, the germination was better in the high temperature rather than in the low temperature. The germination rate was higher in the $30^{\circ}C$. For the carbon source, the germination rate was better at the concentration of 15% regardless of the kinds. In particular, the highest value was observed with glucose. The germination rate was decreased substantially as the increasing with the higher pH. The dynamic germination of pollens was observed at the pH 5. With respect to the growth regulator, the higher concentration of NAA induced the higher the germination rate. $GA_3$ showed a good germination rate in $0.05mg{\cdot}L^{-1}$. Meanwhile, for kinetin, lower concentration increased the germination rate, unlike NAA. The higher concentrations of boric acid degraded the germination rate, and the addition of boric acid of $10mg{\cdot}L^{-1}$ demonstrated higher germination rate than the addition of other growth regulators. Notably, the addition of asparaginic acid exhibited the similar results in all test sectors regardless of concentration, whereas a little higher result was observed in the high concentration sector. Taken together, the results concluded that the boric acid was considered as one of the essential minerals that played an important role on the germination of pollens.

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GM 콩의 도입유전자 이동에 미치는 화분 매개충의 영향 (Influence of insect pollinators on gene transfer from GM to non-GM soybeans)

  • 이범규;김준형;손수인;권순종;박기웅;정영수;이시명
    • 농업과학연구
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    • 제42권3호
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    • pp.159-165
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    • 2015
  • The cultivation area and use of genetically modified (GM) crops have been increased continuously over the world and concerns about the potential risks of GM crops are also increasing. One of the major concern in risk assessment is the possible development of hybrids through interspecific and intergeneric crosses with related species. This study was conducted to investigate the pollinator have an influence on insect-mediated gene transfer from GM soybeans. Hybrid was induced from GM soybeans by honeybee and western flower thrips, and non-GM soybeans were used as pollen receptor. The analysis for gene-flow was conducted by herbicide selection, immunostrip test, and PCR analysis. In the result of the analysis, three hybrids were detected on the distance 15, 75, 105 cm from pollen source in western flower thrips treatment. In honeybee treatment, one hybrid was detected in the farthest distance (300 cm). These results suggested honeybee and western flower thrips have a possibility they can transfer the introduced gene from GM soybeans to non-GM soybeans.

옥수수 기능성 물질의 종류와 변이 (Functional Ingredients of Maize and Their Variation)

  • 김선림;최병한;박승의;문현귀
    • 한국작물학회지
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    • 제41권spc1호
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    • pp.46-68
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    • 1996
  • The objective of this report is to review the up to present papers including 79 references about functional ingredients in maize and their variations. Maize is a good source of such antioxidants as ${\alpha}-,\;{\gamma}-tocopherols,\;{\alpha}-,\;{\beta}-carotene,\;{\beta}-cryptoxanthin$, zeaxanthin, quercetin and chlorogenic acid. Unsaponifiable standard extracts of Zea mays L. (ZML) have a beneficial effect on various dental diseases. Flavonoids, flavonoid biosynthetic pathway and their controling genes were also reviewed in this report. Flavonoids are required for successful fertilization in maize pollen, and maysin has a potent antibiotic activities to earworm and fall armyworm in maize silks. Maize silks have been used in traditional medicine for diuretic, vascular diseases and diabetes, etc. and boiling water extracts of maize silk have hemodynamic effects, but their ingredients still remain obscure.

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SEM/EDX 분석법을 이용한 침착먼지에 대한 분석사례 (Analysis of Deposited Dust Particles using SEM/EDX)

  • 하은지;박정호
    • 한국환경과학회지
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    • 제30권3호
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    • pp.279-287
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    • 2021
  • In this study, the composition and morphology of deposited dust particles with size ranging from a few to tens ㎛ were investigated using SEM/EDX (scanning electron microscopy with energy dispersive x-ray spectrometer). Then deposited dust particles were classified into 8 groups: quartz, aluminosilicates, ca-rich, Fe/Ti oxide, carbon-rich, industrial particle, Fe-rich, and biogenic particle. The sources of deposited dust were high in the order of aluminosilicates 41% > biogenic 18% > Fe-rich 11% > quartz and C-rich 8% > industrial 7% > Fe/Ti oxide 5% > Ca-rich 1%. In particular, the ratio of biogenic particles was relatively high due to influence of pollen. The ratio of carbon-rich was 11% at YM site, 10% at MD site, and 4% at MO site, and the site close to the large emission source was high.

TL과 ESR 분석을 통한 일부 향신료, 장과류 및 화분가공추출물 검지 특성 연구 (Studies on the Applications of TL and ESR Methods for the Detection of Spices, Berry Fruits and Pollen Extract Product)

  • 김규헌;손진혁;강윤정;박혜영;곽지영;이재황;박용춘;조태용;이화정;이상재;한상배
    • 방사선산업학회지
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    • 제7권1호
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    • pp.69-74
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    • 2013
  • This study examined radiation detection characteristics of spices (cumin, caper and turmeric), other small fruits (hut-gae berry and boxthorn), and pollen extract product. Each samples were irradiated at dose of 1, 5, and 10 kGy and analyzed by the thermoluminescence (TL) and electron spin resonance (ESR) methods. To compare between non-irradiated and irradiated food, all samples were irradiated with $^{60}Co$ gamma-ray source. In TL analysis, most of samples could be applied to detect irradiated foods because of showing TL ratio above 0.1. The glow curves examined by TL method were estimated in the range of $150{\sim}250^{\circ}C$ in irradiated samples. In ESR measurements, the intensity of ESR signal (single-line) increased as the increase of irradiation dose. In particular, the specific ESR signals of irradiation-induced radical were detected in hut-gae berry and pollen extract product. As a results, it is considered that TL and ESR methods can be used to detect both hut-gae berry and pollen extract product. But cumin, caper, turmeric and boxthorn irradiated with gamma ray could be detected only by TL method. It is concluded that TL and ESR methods are suitable for detection of irradiated samples and a combined method is recommendable for enhancing the reliability of detection results.

Physical and Chemical Characteristics of Sediments at Bam Islands in Seoul, Korea

  • Han, Mie-Hie;Kim, Jae-Geun
    • Journal of Ecology and Environment
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    • 제29권4호
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    • pp.389-398
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    • 2006
  • To examine sediment characteristics and find anthropogenic effects on riverine wetland ecosystems, paleoecological study was carried out at Bam islands in Seoul. Three hundred cm deep sediment cores were retrieved and dated with the lamination analysis method until 36 cm depth (1986). Sediments were divided into three zones based on the depth profiles of physico-chemical variables: below 160 cm depth (before 1968), between 160 and 40cm depths and above 40cm depth (after 1986). Physico-chemical characteristics were very variable between 160 and 40cm depths and this indicates unstable sedimentation environment. Even though heavy metal concentrations were relatively low, Cd and As contents have increased continuously. Dry mass accumulation rates during $1968{\sim}1986\;and\;1987{\sim}2003$ were 140 and $21\;kg\;m^{-2}\;yr^{-1}$, respectively. This was related to flooding intensity and duration. Bulk density, water content, loss on ignition, N, C, C/N ratio were very similar to other river delta but Ca, Na and K contents were 2 to 4 times higher than others. Heavy metal contents except Pb were lower or similar to those in other studied marshes in Korea. Heavy metal and Mg contents were correlated with each other and this suggests that the source of heavy metals be parent rock. From $^{13}C$ dating dates of organic materials in sediment, it is suggested that organic matter originated from the watershed and flooding intensity in the watershed might be responsible for the source of sediments. This study provides reference data for the comparison of sediment characteristics at islands in river and for the management of Bam islands.

The improvement effect of anti-inflammation of Aronia extract that fermented by Lactic acid bacteria isolated from the fermented seafoods

  • Lim, Jeong-Muk;Choi, Ui-Lim;Lee, Jeong-Ho;Moon, Kwang Hyun;Kim, Dae Geun;Jeong, Kyung Ok;Im, So Yeon;Oh, Byung-Taek
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2018년도 추계학술대회
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    • pp.111-111
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    • 2018
  • Aronia (black chokeberry), a species of berries is source to a very large number of bioactive compounds like polyphenols, flavonoids, anthocyanins, and tannins in comparison to any other species. Owing to its antioxidant, anti-carcinogenic, anti-aging and anti-inflammatory properties. Fermentation- a bioconversion process exploiting the biological metabolic reaction of micro-organisms, has several benefits like improving the efficacy and safety of physiologically active substances, generation of new functional material, improving the adsorption rate and many others. Antigens like pathogens, food, pollen etc., generate a protective immune response in body tissues, and the process be referred to as inflammation. However, this when excessive results in a condition referred to as refractory inflammatory disease, whose incidence is increasing in the recent times, especially amongst children. The current study intended to assess the anti-oxidant activity, presence of polyphenols and anti-inflammatory efficacy of Aronia extract fermented by Lactic acid bacteria isolated from fermented sea foods. Aronia fruits collected from Sunchang, Chonbuk were lyophilized for fermentation. So as to maximise the efficacy of the fermented Aronia extract, the quantitative effects of lactic acid bacteria species, composition of extraction solution, influence of temperature and time on antioxidant activity and total polyphenol contents were investigated using an experimental design. Anti-inflammatory activity was evaluated on NO and cytokine ($TNF-{\alpha}$, IL-6) production in LPS activated Raw 264.7 cells. Results indicated that antioxidant effect and total polyphenol contents were best improved in extract of Aronia fermented by P. pentosaceus. In addition, NO and cytokine ($TNF-{\alpha}$, IL-6) levels were decreased significantly after fermentation. Thus, it was found that the anti-inflammatory activity of Aronia greatly increased after fermentation process using P. pentosaceus.

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식물형태학에서 사용하는 종자식물의 생식구조에 관한 한글 용어의 분석 (Analysis of Some Korean Terminologies on the Reproductive Structures of Seed Plants in Plant Morphology)

  • 이규배
    • 통합자연과학논문집
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    • 제1권3호
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    • pp.195-209
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    • 2008
  • Some Korean terminologies on reproductive structures of seed plants in plant morphology, written incorrectly in many books, were analysed to propose accurately expressed terminologies. 31 books in areas such as general biology, plant biology, plant morphology, and biological dictionaries and glossaries were selected to analyse the accuracy of the terminologies for reproductive structures in gymnosperms, e.g., cone or strobilus, seed (or ovulate) cone and pollen cone, and conifer(s) or coniferous plants, and for flower structures in angiosperms, e.g., corolla, anther, filament, pistillate (or female) flower and staminate (or male) flower, apocarpous, and syncarpous. The definition and etymology of the terminologies were traced in 4 textbooks of plant anatomy and 2 dictionaries of biology and botany written in English. On the basis of the definition, etymology, and principles for terminology formation according to the International Organization for Standardization (ISO 704:2000), reasonably expressed Korean terminologies were proposed. All of the 8 terminologies examined in this study were included in the glossary of biological terminologies, published by the Korean Association of Biological Sciences in 2005, and designated as an editorial source for science and biology textbooks for middle and high schools by Ministry of Education in 2007. However, the only 1 of the 8 terminologies described in the glossary were consistent with the proposed expression in the present study. These inconsistencies indicated the need for a reassessment of this glossary of biological terminologies. The newly proposed terminologies would facilitate mutual understanding between teachers and students of plant biology.

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Morphological variables restrict flower choice of Lycaenid butterfly species: implication for pollination and conservation

  • Mukherjee, Subha Shankar;Hossain, Asif
    • Journal of Ecology and Environment
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    • 제45권4호
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    • pp.305-312
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    • 2021
  • Background: Butterflies make an important part for plant-pollinator guild. These are nectar feeder or occasionally pollen feeder and thus proboscis of the butterfly species are considered as one of the most important variable in relation to the collection of food from plants. In butterfly-plant association, nectar source is principally determined by quality of nectar, corolla length, and nectar quantity. For the butterfly, nectar uptake is determined by proboscis length because flowers with long corolla restrict butterfly species containing shorter proboscis. Empirical studies proved that butterfly species with high wing loading visit clustered flowers and species with low wing loading confined their visit to solitary or less nectar rich flowers. The present study tries to investigate the flower preference of butterfly species from Lycaenidae family having very short proboscis, lower body length, lower body weight and wing span than the most species belonging from Nymphalidae, Pieridae, Papilionidae, and Hesperiidae. Results: Butterflies with shorter proboscis cannot access nectar from deeper flower. Although they mainly visit on less deeper flower to sucking nectar, butterflies with high wing loading visits clustered flowers to fulfill their energy requirements. In this study, we demonstrated flower choice of seven butterfly species belonging to Lycanidiae family. The proboscis length maintains a positive relationship with body length and body weight. Body length maintains a positive relationship with body weight and wing span. Wing span indicate a strong positive relationship with body weight. This study proved that these seven butterfly species namely Castalius rosimon (CRN), Taracus nara (TNA), Zizinia otis (ZOT), Zizula hylax (ZHY), Jamides celeno (JCE), Chilades laius (CLA), and Psuedozizeeria maha (PMA) visit frequently in Tridax procumbens (TPR), Ocimum americanum (OAM) and Syndrella nodiflora (SNO). The species do not visit Lantana camara (LCA) and Catharanthus roseus (CRO) plants. Conclusion: The present study proved that butterfly species visits frequently in Tridax procumbens (TPR), Ocimum americanum (OAM) but less frequently in Syndrella nodiflora (SNO). So, that study determined the butterfly species helps in pollination of these herbs that in turn helps the conservation of these butterfly species.