• Korean Journal of Plant Resources
• /
• v.16 no.1
• /
• pp.89-98
• /
• 2003

Pollens of six taxa in the genus Atractytis L. confused with Atractylodes DC. were investigated by the light and the scanning electron microscope. Pollen grains of six taxa in this genus were monads, 46.5-66.7$\mu\textrm{m}$ in length of polar axis, 43.6-60.47$\mu\textrm{m}$ in equatorial width. Aperture was tri-colporate, 19.6-29.1 $\mu\textrm{m}$ in colpus length,7.3-11.0$\mu\textrm{m}$ in colpus width and 6.4-10.6$\mu\textrm{m}$ in porus diameter. The exine was composed of three layers(foot layer, columellne, tectum), and 5.2-8.3 $\mu\textrm{m}$ in thickness. The surface sculpturing pattern was echinate, the spines were 1.4-5.8$\mu\textrm{m}$ in length and 6-33 per 20$\mu\textrm{m}$$^2$ in distribution numbers over the surface. Atractylis L. was not distinguished from Atractylodes DC. by pollen morphology, especially the pollens of Atractylis arabica, A. aristata, A. carduus, A. microcephala were very similar to that of Atractylodes. But its taxonomic significance was different, the taxa of Atractylodes DC. were divided into 2 types by only pollen size, but the treated taxa in this study were divided into various types by pollen size, shape of equatorial view, surface sculpturing and spine size. The characteristics of spine size and shape of equatorial view in A. cancellata and A. prolifera were very useful for consideration of evolutionary trends in this genus.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.49 no.5
• /
• pp.407-409
• /
• 2004

Thirty-nine out of eighty-five barley varieties/strains survived until heading stage in the saline experimental field $(0.03-0.05\%\;salt)$ and they were used for pollen study. Light and scanning electron microscopic observations revealed two distinctive types of barley pollens: one transparent and small in size and the other dark and larger. In addition, both types of pollens were stained with Alexander's stain and it was found that the smaller and transparent pollen was cytoplasm-devoid (CD) while the larger pollen was cytoplasm-rich (CR). Sixteen out of 39 barley varieties/lines grown in the saline soil had CR pollens, which were rarely observed in the barley plants grown in the non-saline soil. Moreover, it was observed that salt stress severely reduced seed setting in the varieties having degenerated pollens. These results suggest that salt stress affects the fertility of barley pollen. The sterile pollen was undersized and lack of cytoplasm probably due to abortion. Furthermore, a varietal difference existed in the response of pollen development to salt stress.

• Applied Microscopy
• /
• v.35 no.4
• /
• pp.74-83
• /
• 2005

The ontogeny of pollen wall in Panax ginseng was studied with transmission and scanning electron microscopy from early tetrad stage until pollen maturity. Initial indication of exine development is undulation of plasma membrane for the preparation of bacular mound. The first recognizable structure of the pollen wall is the cellulosic primexine which is formed outside of the plasma membrane while microspore tetrads are still surrounded by callose wall. As development proceeds, foot-layer and baculum differentiation, callose dissolution and exine formation were progressed. During this process, sporopollenin is deposited into the exine, and then endexine development was followed. The intine, innermost pollen wall layer, is developing form hypertrophic Golgi vesicles. The thickness of exine is very even on all along the pollen wall, but intine thickness of apertural region is thicker than that of nonapertural region. Mature pollen of ginseng is $20{\mu}m$ in size, tricolpate and shows fine reticulate sculpturing.

• Journal of Ginseng Research
• /
• v.21 no.2
• /
• pp.85-90
• /
• 1997

This study was carried out to clarify the cause of incompatibility in interspecific hybrid plant between Panax ginseng and p. quinquefolium. The floral structure of F,(p.g. x p.q.) hybrid was normal because the redundant anther was 0.2 mm longer than pistil in Fl hybrid and the size and structure of redundant carpel in F, hybrid were similar to P. ginseng and p. quiquefolium Pollens of $F_1$ hybrid did not germinate on stigma of P-quinquefolium but germinated well on stigma of P. ginseng. Pollen tube was able to penetrate styles completely and seed harvest rate was 16.8% in field. However on stigma of $F_1$ hybrid, Pollen did not germinate when P. ginseng was used as male Parent. In addition, the growth of pollen tube was halted on style and seed was not set when P qlfinquefoEi2a was used as male Parent. These suggest that the inhibitor of pollen germination present on stigma caused $F_1$ hybrid sterility. It took 5 hours for pollen grains to germinate, 12 hours to arrive at in trance of ovule, 16 hours to penetrate micropyles in Panax ginseng.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.47 no.1
• /
• pp.62-67
• /
• 2002

An efficient system of rice microspore culture could contribute to the production of genetically modified rice. The microspores were isolated by mechanical or shed methods. The number of microspores per 100 anthers isolated at uninucleate stage was higher than (or similar to) those at binucleate stage in isolation method with pestle or spatular, but microspore divisions were not easily observed on both stages. On the other hand, pollen division in shed pollen culture was observed more frequently at uninuclear than at binuclear stage. Cold pretreatment at 1$0^{\circ}C$ for 10 days resulted in the best multicellular division to produce microcalli at 12.5% efficiency in shed microspores. Heat shock at 33$^{\circ}C$ for one hour before or after pollen shedding enhanced cell division and callus formation. Out of twelve green regenerants, two were haploids and ten were diploids based on the chromosome analysis of root tips. The size of stoma was 12$^{m}$ m in haploids and 15 ${\mu}{\textrm}{m}$ in diploids determined by scanning electron microscope (SEM).

• Korean Journal of Plant Resources
• /
• v.20 no.2
• /
• pp.193-200
• /
• 2007

Pollen grains of seven taxa on the family Pyrolaceae, known to be distributed in Korea (Pyrola dahurica(Andreses) Kom., P. incarnata (DC.) Fisch. ex Kom., P. japonica Klenze ex Alefeld, P. minor L., P. renifolia Max., Orthilia secunda (L.) House, Chimaphila japonica Miq.), were examined by the light and the scanning electron microscope in order to evaluate their taxonomic significances. The pollen units of genus Pyrola, Orthilia, Chimaphila were tetrad, monad, polyad respectively. This characteristics was good character for delimiting the genus, and Orthilia secunda (L.) House was more proper scientific name than Pyrola secunda L. based upon this character. Pollen size, tricolporate length, surface sculpturing pattern were different slightly among the treated taxa, and surface sculpturing patterns of Pyrola dahurica, P. incarnate, P. renifolia, Chimaphiza japonica were different from previous reports. Evolutionary trends of pollen grains in Pyrolaceae were inferred from the pollen units and aperture shapes.

• Journal of Plant Biology
• /
• v.22 no.1_2
• /
• pp.45-51
• /
• 1979

From 32 angiosperm species, pollen surface characters such as sculpture pattern, roughness, oil abundance and stickiness were subjectively scored, and compared with size and weight of the grains, and pollination mechanisms. There was a linear relationship between size and weight of the grains. Animal-pollinated grains showed significantly higher roughness, oil abundance and stickiness than wind-pollinated grains. In the former, the grain size was highly correlated with roughness and oil abundance. No such relationship was found in the latter. It was suggested, therefore, that the large the animal-pollinated grains, the greater is the stickiness required, where the stickiness depends on the surface soil abundance and roughness. No functional significance of the sculpture pattern could not be detected in this study.

• KSBB Journal
• /
• v.17 no.6
• /
• pp.582-585
• /
• 2002

In an effort to use plant biotechnology for the production of biopharmaceuticals, pollens collected from lily (Lilium longiflorum) were grown in vitro and transformed with a PCR-amplified 1.7 kb cDNA encoding human tissue plasminogen activator (tPA) using Agrobacterium via a vacuum infiltration process. Western blotting showed that transgenic lily pollen tubes selected on kanamycin for 16 hrs expressed a tPA protein with a size similar to the human standard, suggesting their possible use as a disposable host for rapid foreign protein production.

• Journal of Plant Biology
• /
• v.35 no.3
• /
• pp.205-210
• /
• 1992

Size>; and sculpture pattern of pollen grains in seven species and two varieties of the genus Lilium in Korea were examined by LM and SEM, and their evolutionary trends were discussed. Lilium species in Korea can be divided into two groups based on pollen morphology: one is small reticulate group with small pollen grains and lumens, which includes L. concolor var. pulchellum and L. callosum; the other is large reticulate group with large pollen grains and lumens, which includes remaining taxa. It is thought that small reticulate group is more primitive than large reticulate group on the basis of tectum coverage. According to the above results and some morphological characters of salles and perianth segments, L. concolor var. pulchellum and L. callosum are very closely related, and they would be treated under the same subsection.ection.

• Journal of Korea Foresty Energy
• /
• v.19 no.1
• /
• pp.38-48
• /
• 2000

This study was initiated to investigate similarity and intraspecific variation in pollen morphology of korean subgenus. lepidobalanus Endl, of the genus Quercus L. The parameters measured were polar axis length (PL), equatorial width (EW), colpus length (CL) and copus width (CW), and PE (PL/EW) retios were calculated. The cluster analysis based on these variables of this result showed that the similarity between Q. acutissima Carruther and Q. variabilis Blume was the highest. Wide variation in the pollen grain size of Quercus dentata Thunb. ex Murray might imply, the existence of polyploid and/or aneuploid forms. Studies of ploidy levels within species are recommended for Lepidobalanus species.