The presence of Chrysanthemum stunt viroid (CSVd) in seed and pollen of diseased chrysanthemum was demonstrated. In seeds infected male parent from crosses in May, CSVd was transmitted to 6.7% of the progeny seedlings, whereas if the female parent was infected, CSVd transmission rate was between 46.9 and 75.7%. A relatively high incidence of 94.4 to 96.0% seed transmission occurred when both parents were infected. In seeds infected male parent from crosses in December, no progeny seedlings were infected with CSVd, whereas if the female parent did, CSVd transmission rate was 1.5%. When both parents were infected, 6.9% seed transmission was occurred. The seed transmission rate depended on the temperature when the crosses were made. CSVd was not detected in the non-infected female parent pollinated with infected pollen but was transmitted to the progenies. This is the first report of seed-borne transmission of CSVd in chrysanthemum.
Many flowering plants possess genetically controlled self -incompatibility (SI) system that prevents inbreeding and promotes outcrosses. SI is usually controlled by a single, multiallelic S-locus. In gametophytically controlled system, SI results when the S-allele of the pollen is matched by one of the two S-alleles in the style, while in the sporophytic system self-incompatible reaction occurs by the interaction between the pistil genotype and genotype of, not the pollen, but the pollen parent In the former system the self-incompatible phenotype of pollen is determined by the haploid genome of the pollen itself but in the latter the pollen phenotype is governed by the genotype of the pollen parent along with the occurrence of either to-dominant or dominant/recessive allelic interactions. In the sporophytic type the inhibition reaction occurs within minutes following pollen-stigma contact, the incompatible pollen grains usually failing to germinate, whereas in gametophytic system pollen tube inhibition takes place during growth in the transmitting tissue of the style. Recognition and rejection of self pollen are the result of interaction between the S-locus protein in the pistil and the pollen protein. In the gametophytic SI the S-associated glycoprotein which is similar to the fungal ribonuclease in structure and function are localized at the intercellular matrix in the transmitting tissue of the style, with the highest concentration in the collar of the stigma, while in the sporophytic SI deposit of abundant S-locus specific glycoprotein (SLSG).is detected in the cell wall of stigmatic papillae of the open flowers. In the gametophytic system S-gene is expressed mostly at the stigmatic collar the upper third of the style length and in the pollen after meiosis. On the other hand, in the sporophytic SI S-glycoprotein gene is expressed in the papillar cells of the stigma as well as in e sporophytic tape is cells of anther wall. Recognition and rejection of self pollen in the gametophytic type is the reaction between the ribonuclease in the transmitting tissue of the style and the protein in the cytoplasm of pollen tube, whereas in the sporophytic system the inhibition of selfed pollen is caused by the interaction between the Sycoprotein in the wall of stigmatic papillar cell and the tapetum-origin protein deposited on the outer wall of the pollen grain. The claim that the S-allele-associated proteins are involved in recognition and rejection of self pollen has been made merely based on indirect evidence. Recently it has been verified that inhibition of synthesis of S$_3$ protein in Petunia inflata plants of S$_2$S$_3$ genotype by the antisense S$_3$ gene resulted in failure of the transgenic plant to reject S$_3$ pollen and that expression of the transgenic encoding S$_3$ protein in the S$_1$S$_2$ genotype confers on the transgenic plant the ability to reject S$_3$ pollen. These finding Provide direct evidence that S-proteins control the s elf-incompatibility behavior of the pistil.
This study was carried out to clarify the cause of incompatibility in interspecific hybrid plant between Panax ginseng and p. quinquefolium. The floral structure of F,(p.g. x p.q.) hybrid was normal because the redundant anther was 0.2 mm longer than pistil in Fl hybrid and the size and structure of redundant carpel in F, hybrid were similar to P. ginseng and p. quiquefolium Pollens of $F_1$ hybrid did not germinate on stigma of P-quinquefolium but germinated well on stigma of P. ginseng. Pollen tube was able to penetrate styles completely and seed harvest rate was 16.8% in field. However on stigma of $F_1$ hybrid, Pollen did not germinate when P. ginseng was used as male Parent. In addition, the growth of pollen tube was halted on style and seed was not set when P qlfinquefoEi2a was used as male Parent. These suggest that the inhibitor of pollen germination present on stigma caused $F_1$ hybrid sterility. It took 5 hours for pollen grains to germinate, 12 hours to arrive at in trance of ovule, 16 hours to penetrate micropyles in Panax ginseng.
Lee, Sun Yi;Kim, Seung Yu;Kim, Dae Young;Jeong, Ho Jeong;Rho, Il Rae
Journal of agriculture & life science
/
v.50
no.2
/
pp.45-52
/
2016
In order to select excellent cross parents for development new cultivar of low temperature tolerant, combining ability was conducted by 24 cross combinations obtained from crosses between 'Dahong', 'Gamhong', 'Maehyang', 'Seolhyang' as seed parents and 'Dahong', 'Gamhong', 'Maehyang', 'Sugyeong', 'Sunhong', 'Wongyo 3111' as pollen parents. The results showed that two cultivars of 'Dahong', 'Gamhong' were not suitable for seed parents. Because average fruit weight in case of 'Dahong' as a seed parent was the tendency to become the smallest in total cross combinations, survival ratio in case of 'Gamhong' as a seed parent was the lowest in total cross combinations. And fruit hardness in case of 'Sunhong' as a pollen parent was the tendency to low, incidence of malformed fruit in case of 'Sugyeong' as a pollen parent was the tendency to be increased. Therefore, two cultivars of 'Sunhong', 'Sugyeong' was also not suitable for pollen parents. But In case of 'Maehyang' and 'Seolhyang' as seed parents, germination percent and survival rate were relatively higher than other seed parents, fruit quality and yield had also excellent. Therefore, there could be selected to 'Maehyang' and 'Seolhyang' as seed parents and 'Maehyang', 'Seolhyang', 'Wongyo3111' as pollen parents. Especially, the crosses 'Maehyang' × 'Seolhyang', 'Seolhyang' × 'Maehyang', 'Seolhyang' × 'Wongyo3111' were suitable for cross combination for development new cultivar of low temperature tolerant to be demonstrated the excellence as cross parents in sugar contents, fruit weight, fruit hardness and yield.
One row of pollen parent to two rows of seed parent (1:2), 2:4 and solid (1:2) planting patterns (PP) were compared in seed parent densities of 3,500, 5,000 and 6,500 plants per 10 ares to determine effects of PP and plant density on growths of seed and pollen plants, and seed yield of seed parent of modified single cross corn hybrid. Planting pattern did not significantly affect agronomic characteristics of seed plant except ear number per 100 plants and seed yield which were greater in solid and 1:2 PP than in 2:4 PP. Significant PP x plant density interaction did not exist for agronomic characteristics of seed parent. In the seed parent, plant height and 100 kernel weight were not affected by plant density, but ear height, ear number per 100 plants, and kernel number per ear were linearly decreased with increased plant densities. Seed yield ranged from 330 to 460 kg per 10 ares and overall yield response to plant density was quadratic. Tassel length and spikelet number per tassel of the pollen parent were significantly affected by PP and plant density. Significant PP x plant density interaction existed for tassel length and spikelet number per tassel. Tassel length and spikelet number per tassel were greater in 1:2 and 2:4 PP compared to solid PP and were greatly reduced with increased plant densities in solid and 1:2 PP. The results indicated that 1:2 or 2:4 PP at around 5,000 plants per 10 ares for seed parent would be suitable for seed production of modified single cross com hybrid.
The effect of directions (north to south vs east to west) of planting row, planting intensities (10/15, 15/15, 20/15, 25/15 cm/cm) and the number of rows consecutively (1,2, 3,4) for both the CMS and pollen parent in alternation, on the seed yield of CMS of rice was tested with a breeding line V20A/Iri342$\^$*8/. When the planting row was made in vertical direction to the wind direction during anthesis, the seed yield was higher than the other direction regardless the planting densities and number of rows consecutive. Seed yield increased as high as 46.9%. The higher planting intensities up to 10/15 cm/cm yielded the higher hybrid seeds. The highest yield was 81.2 kg/ha. The yield variation among plants within a plot and among the plants within a replication was relatively large. Increased pollen parent row numbers caused increased grain fertility per plant, but the seed yield was increased by reduced pollen parent row number. Reduced CMS parental row numbers caused increased grain fertility per plant, but the seed yield was increased by increased CMS parental row numbers. Considering the hybrid seed yield, parental seed yield and operational convenience, 4 rows of CMS and 1 row of pollen parent in alternate with 20/15 or 25/15 (cm/cm) planting densities seemed to be the rational layout.
Park, In-Sook;Suh, Dong-Hee;Hwang, Yoon-Jung;Park, Song-Kyung;Kang, Si-Yong;Rhee, Hye-Kyung;Lim, Ki-Byung
Korean Journal of Breeding Science
/
v.41
no.4
/
pp.451-455
/
2009
Lilium lancifolium, which is one of the Korean native lilies, possesses several useful genetic characteristics such as growth vigor, bulbil formation and resistance to Fusarium. Hybridization was performed to insert useful traits of Asiatic hybrid "Dreamland" as the male parent with upward direction and dark brown spotless on the petal into L. lancifolium as the female parent. The rate of pollen germination of L. lancifolium and "Dreamland" were 30% and 60%, respectively. The pollen germination ratio of $F_1$ progenies at flowering date was 25-57%, which is between the parents'pollen germination ratio. The individual flowering time was between 113 days and 131 days after planting. Of 99% of all plants, the leaf shape index was more than five, which means that the leaf shape of $F_1$ plants is more influenced by female than male parent. The flower color was separated into four types. 51.5% of the progenies showed orange and 44.2% showed light orange, respectively. The colors seemed to be highly influenced by female parents. Mixed colors of parents, orange yellow and light yellow orange, were 3.1%, 1.2%, respectively. Flowering directions were segregated by three different ways, upside, side, and downside. Side direction, which is medium type between female and male parent, was 73%. All progenies showed lots of spot on petals except one offspring. The percentage of bulbil formation on leaf axil was 49.4%. Among those, the maximum number of bulbil formation was 25. The mean number of bulbil formation per plant was five.
This study identified a new male-sterile germplasm of Chinese jujube, named male-sterile No. 2 (JMS2), and achieved controlled hybridization using that germplasm as the female parent. The anthers of JMS2 before flower bud opening became shrunken, dingy yellow and much smaller than normal ones, and they changed to brown after anthesis. No pollen was observed in anthers of JMS2 and its male-sterile trait remained stable over different years. A total of 1,642 fruits were obtained from ten intra- and interspecific cross combinations via controlled hybridization from 2008 to 2012 using JMS2 as the female parent. Of those, 27.3% produced seeds, on average (0-72.6%). The rate of fruit with seed (RFS) was significantly different between cross combinations or years. Compared to other cross combinations, the RFS in the combination of JMS2 ${\times}$ 'Xingguang' (a Chinese jujube cultivar with high resistance to jujube witches' broom disease) and JMS2 ${\times}$ 'Xing16' (a sour jujube genotype) remained high in different years and reached means of 48.7 and 58.1%, respectively. Finally, 150 plantlets were regenerated from immature embryos, and 51 of them were randomly selected and identified to be authentic hybrids using amplified fragment length polymorphism (AFLP) markers. This is the first report of hybrids obtained from a cross between Chinese jujube and sour jujube.
Suh, Dong Hee;Hwang, Yoon Jung;Park, In Sook;Park, Song Kyoung;Chung, Jae-Dong;Lim, Ki Byung
FLOWER RESEARCH JOURNAL
/
v.16
no.2
/
pp.118-123
/
2008
For effective production of interspecific hybrids of Lilium and valuable new cultivars, we used Lilium Asiatic hybrid 'Tiny Ghost' as the female parent, and Oriental group, and Longiflorum group and Martagon as the male parents. Percentage pollen germination in Oriental hybrid 'Sorbonne' was the highest with 64%. As results of using normal style pollination and cut style pollination methods, all seed sets were formed in normal style pollinated treatment, except in 'Aktiva'. Pollen germination percent of Longiflorum hybrid 'Norina' was higher than that 'Gelria'. However, all crossings using 'Norina' failed to set seeds. Corresponding fertilization ratio was different from genetic compatibility, even in the same group. From the crossing using 'Aktiva' as the male parent one bulb was obtained derived from one embryo sac. And using 'Sorbonne' as the male parent, one plantlet was obtained after four ovule cultures. Crossing using 'Gelria' as the male parent five embryos, 15 embryo sacs, and seven ovules per one ovary were obtained. Among them, 18 plantlets germinated, with the germination percent of 66.7%. Crossings using L. hansonii, Martagon group, as the male parent resulted in 40% germination from five ovules.
Cheung, Hyon Pae;Chon, Sang Kun;Kim, Mal Sook;Kim, Chung Suk
Journal of Korean Society of Forest Science
/
v.45
no.1
/
pp.51-61
/
1979
The chromosome behavior and it's synapsis in the meiosis of pollen mother cell were studied on Populus alba L. as a female parent tree, Populus glandulosa Uyeki as a male parent tree and their hybrid, Populus alba x glandulosa. 1. At metaphase I, the number of nuclear plates with early separation chromosome were observed with the lowest proportion of 11.0% in Populus glandulosa and with the highest proportion of 13.0% in Populus alba${\times}$glandulosa. 2. At metaphase II, early separation chromosomes appeared with the frequency of 11.0% in Populus alba${\times}$glandulosa. But the frequency was not different with those of the parental trees. 3. At anaphase I, lagging chromosomes appeared with some high rate of 11.6% in Populus alba${\times}$glandulosa and yet the number of chromosome bridges in populus alba x glandulosa almost were not different with the partental trees. 4. At anaphase II, lagging chromosomes appeared with some high frequency of 10.2% in Populus alba${\times}$glandulosa and the chromosome bridges in Populus glandulosa appeared with the highest frequency in all studied trees. 5. The frequency of abnormal pollen sporad was the highest value of 8.2% in Populus alba${\times}$glandulosa among the studied trees. With the results, it might be assured that the chromosome segregation and it's synapsis behaved normally in Populus alba, Populus glandulosa and Populus alba x glandulosa, and so all the studied trees could produced normal pollens.
본 웹사이트에 게시된 이메일 주소가 전자우편 수집 프로그램이나
그 밖의 기술적 장치를 이용하여 무단으로 수집되는 것을 거부하며,
이를 위반시 정보통신망법에 의해 형사 처벌됨을 유념하시기 바랍니다.
[게시일 2004년 10월 1일]
이용약관
제 1 장 총칙
제 1 조 (목적)
이 이용약관은 KoreaScience 홈페이지(이하 “당 사이트”)에서 제공하는 인터넷 서비스(이하 '서비스')의 가입조건 및 이용에 관한 제반 사항과 기타 필요한 사항을 구체적으로 규정함을 목적으로 합니다.
제 2 조 (용어의 정의)
① "이용자"라 함은 당 사이트에 접속하여 이 약관에 따라 당 사이트가 제공하는 서비스를 받는 회원 및 비회원을
말합니다.
② "회원"이라 함은 서비스를 이용하기 위하여 당 사이트에 개인정보를 제공하여 아이디(ID)와 비밀번호를 부여
받은 자를 말합니다.
③ "회원 아이디(ID)"라 함은 회원의 식별 및 서비스 이용을 위하여 자신이 선정한 문자 및 숫자의 조합을
말합니다.
④ "비밀번호(패스워드)"라 함은 회원이 자신의 비밀보호를 위하여 선정한 문자 및 숫자의 조합을 말합니다.
제 3 조 (이용약관의 효력 및 변경)
① 이 약관은 당 사이트에 게시하거나 기타의 방법으로 회원에게 공지함으로써 효력이 발생합니다.
② 당 사이트는 이 약관을 개정할 경우에 적용일자 및 개정사유를 명시하여 현행 약관과 함께 당 사이트의
초기화면에 그 적용일자 7일 이전부터 적용일자 전일까지 공지합니다. 다만, 회원에게 불리하게 약관내용을
변경하는 경우에는 최소한 30일 이상의 사전 유예기간을 두고 공지합니다. 이 경우 당 사이트는 개정 전
내용과 개정 후 내용을 명확하게 비교하여 이용자가 알기 쉽도록 표시합니다.
제 4 조(약관 외 준칙)
① 이 약관은 당 사이트가 제공하는 서비스에 관한 이용안내와 함께 적용됩니다.
② 이 약관에 명시되지 아니한 사항은 관계법령의 규정이 적용됩니다.
제 2 장 이용계약의 체결
제 5 조 (이용계약의 성립 등)
① 이용계약은 이용고객이 당 사이트가 정한 약관에 「동의합니다」를 선택하고, 당 사이트가 정한
온라인신청양식을 작성하여 서비스 이용을 신청한 후, 당 사이트가 이를 승낙함으로써 성립합니다.
② 제1항의 승낙은 당 사이트가 제공하는 과학기술정보검색, 맞춤정보, 서지정보 등 다른 서비스의 이용승낙을
포함합니다.
제 6 조 (회원가입)
서비스를 이용하고자 하는 고객은 당 사이트에서 정한 회원가입양식에 개인정보를 기재하여 가입을 하여야 합니다.
제 7 조 (개인정보의 보호 및 사용)
당 사이트는 관계법령이 정하는 바에 따라 회원 등록정보를 포함한 회원의 개인정보를 보호하기 위해 노력합니다. 회원 개인정보의 보호 및 사용에 대해서는 관련법령 및 당 사이트의 개인정보 보호정책이 적용됩니다.
제 8 조 (이용 신청의 승낙과 제한)
① 당 사이트는 제6조의 규정에 의한 이용신청고객에 대하여 서비스 이용을 승낙합니다.
② 당 사이트는 아래사항에 해당하는 경우에 대해서 승낙하지 아니 합니다.
- 이용계약 신청서의 내용을 허위로 기재한 경우
- 기타 규정한 제반사항을 위반하며 신청하는 경우
제 9 조 (회원 ID 부여 및 변경 등)
① 당 사이트는 이용고객에 대하여 약관에 정하는 바에 따라 자신이 선정한 회원 ID를 부여합니다.
② 회원 ID는 원칙적으로 변경이 불가하며 부득이한 사유로 인하여 변경 하고자 하는 경우에는 해당 ID를
해지하고 재가입해야 합니다.
③ 기타 회원 개인정보 관리 및 변경 등에 관한 사항은 서비스별 안내에 정하는 바에 의합니다.
제 3 장 계약 당사자의 의무
제 10 조 (KISTI의 의무)
① 당 사이트는 이용고객이 희망한 서비스 제공 개시일에 특별한 사정이 없는 한 서비스를 이용할 수 있도록
하여야 합니다.
② 당 사이트는 개인정보 보호를 위해 보안시스템을 구축하며 개인정보 보호정책을 공시하고 준수합니다.
③ 당 사이트는 회원으로부터 제기되는 의견이나 불만이 정당하다고 객관적으로 인정될 경우에는 적절한 절차를
거쳐 즉시 처리하여야 합니다. 다만, 즉시 처리가 곤란한 경우는 회원에게 그 사유와 처리일정을 통보하여야
합니다.
제 11 조 (회원의 의무)
① 이용자는 회원가입 신청 또는 회원정보 변경 시 실명으로 모든 사항을 사실에 근거하여 작성하여야 하며,
허위 또는 타인의 정보를 등록할 경우 일체의 권리를 주장할 수 없습니다.
② 당 사이트가 관계법령 및 개인정보 보호정책에 의거하여 그 책임을 지는 경우를 제외하고 회원에게 부여된
ID의 비밀번호 관리소홀, 부정사용에 의하여 발생하는 모든 결과에 대한 책임은 회원에게 있습니다.
③ 회원은 당 사이트 및 제 3자의 지적 재산권을 침해해서는 안 됩니다.
제 4 장 서비스의 이용
제 12 조 (서비스 이용 시간)
① 서비스 이용은 당 사이트의 업무상 또는 기술상 특별한 지장이 없는 한 연중무휴, 1일 24시간 운영을
원칙으로 합니다. 단, 당 사이트는 시스템 정기점검, 증설 및 교체를 위해 당 사이트가 정한 날이나 시간에
서비스를 일시 중단할 수 있으며, 예정되어 있는 작업으로 인한 서비스 일시중단은 당 사이트 홈페이지를
통해 사전에 공지합니다.
② 당 사이트는 서비스를 특정범위로 분할하여 각 범위별로 이용가능시간을 별도로 지정할 수 있습니다. 다만
이 경우 그 내용을 공지합니다.
제 13 조 (홈페이지 저작권)
① NDSL에서 제공하는 모든 저작물의 저작권은 원저작자에게 있으며, KISTI는 복제/배포/전송권을 확보하고
있습니다.
② NDSL에서 제공하는 콘텐츠를 상업적 및 기타 영리목적으로 복제/배포/전송할 경우 사전에 KISTI의 허락을
받아야 합니다.
③ NDSL에서 제공하는 콘텐츠를 보도, 비평, 교육, 연구 등을 위하여 정당한 범위 안에서 공정한 관행에
합치되게 인용할 수 있습니다.
④ NDSL에서 제공하는 콘텐츠를 무단 복제, 전송, 배포 기타 저작권법에 위반되는 방법으로 이용할 경우
저작권법 제136조에 따라 5년 이하의 징역 또는 5천만 원 이하의 벌금에 처해질 수 있습니다.
제 14 조 (유료서비스)
① 당 사이트 및 협력기관이 정한 유료서비스(원문복사 등)는 별도로 정해진 바에 따르며, 변경사항은 시행 전에
당 사이트 홈페이지를 통하여 회원에게 공지합니다.
② 유료서비스를 이용하려는 회원은 정해진 요금체계에 따라 요금을 납부해야 합니다.
제 5 장 계약 해지 및 이용 제한
제 15 조 (계약 해지)
회원이 이용계약을 해지하고자 하는 때에는 [가입해지] 메뉴를 이용해 직접 해지해야 합니다.
제 16 조 (서비스 이용제한)
① 당 사이트는 회원이 서비스 이용내용에 있어서 본 약관 제 11조 내용을 위반하거나, 다음 각 호에 해당하는
경우 서비스 이용을 제한할 수 있습니다.
- 2년 이상 서비스를 이용한 적이 없는 경우
- 기타 정상적인 서비스 운영에 방해가 될 경우
② 상기 이용제한 규정에 따라 서비스를 이용하는 회원에게 서비스 이용에 대하여 별도 공지 없이 서비스 이용의
일시정지, 이용계약 해지 할 수 있습니다.
제 17 조 (전자우편주소 수집 금지)
회원은 전자우편주소 추출기 등을 이용하여 전자우편주소를 수집 또는 제3자에게 제공할 수 없습니다.
제 6 장 손해배상 및 기타사항
제 18 조 (손해배상)
당 사이트는 무료로 제공되는 서비스와 관련하여 회원에게 어떠한 손해가 발생하더라도 당 사이트가 고의 또는 과실로 인한 손해발생을 제외하고는 이에 대하여 책임을 부담하지 아니합니다.
제 19 조 (관할 법원)
서비스 이용으로 발생한 분쟁에 대해 소송이 제기되는 경우 민사 소송법상의 관할 법원에 제기합니다.
[부 칙]
1. (시행일) 이 약관은 2016년 9월 5일부터 적용되며, 종전 약관은 본 약관으로 대체되며, 개정된 약관의 적용일 이전 가입자도 개정된 약관의 적용을 받습니다.