• 한국식품과학회지
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• 제17권2호
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• pp.121-127
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• 1985

Ciguatoxin 구조 연구의 일환으로 파랑비늘돔 중에 서 독성분을 추출하였는데 Scarus sordidus만 근육과 내장에 독이 있었으나, S.frenatus, S.scaber and S.pectorlis에는 모두 독이 없었다. 실리스산컬럼에서 얻은 조독을 DEAE-셀루로즈 컬럼상에서 콜로로포름, 클로로포름-메타놀 (1 : 1) 및 메타놀로 전개하였더니 클로로포름 유출액(ST-1) 과 클로로포름-메타놀(1 : 1) 유출액에서 독성분이 발견되었고, 메타놀 유출액에서는 독성분이 검출되지 않았다. ST-1과 ST-2을 silica gel 60F-254에 spot하여 클로로포름-메타놀-물-식초산 (90 : 9.5 : 0.2 : 0.3)으로 전개하였더니, 그Rf값이 각각 0.60-0.75, 0.30-0.54였다. DEAE-셀루로즈 컬럼에서 얻은 ST-1을 재재크로마토그라피 하여도 역시 ST-1과 ST-2로 나누어졌다. 이렇게 얻은 ST-1과 ST-2는 세파텍스 LH-20에 흡착시켜, 클로로포름-메타놀(1 : 1) 용매로 전개할 때, 머무름시간이 모두 같았고, 역상 LiChrosorb RP-18를 이용한 HPLC상에서도 머무름시간이 동일하였다. 또 순수한 $ST-1\;(1.10\;{\mu}g)$ 그리고 이것을 1 N NaOH로 처리한 것 $(1.13\;{\mu}g)$을 활성도V인 알루미나 컬럼에 흡착시켜 클로로포름-메타놀 혼합액으로 전개하였더니 알카리처리를 하지 않는 경우는 $0.35\;{\mu}g)$가 클로로포름-메타놀의 9 : 1, 1 : 1 유출액에서, $(0.55\;{\mu}g)$가 메타놀-물 (1 : 1) 유출액에서 검출되었으나, 알카리처리를 한 경우는 처음 독성의 50%가 소실되어, 클로로포름-메타놀 (9 : 1) 유출액에서 $(0.21\;{\mu}g)$, 메타놀-물 (1 : 1) 유출액에서 $(0.80\;{\mu}g)$ 검출되었다. 하면 morey eel내장에서 얻은 독물질도 DEAE-셀루로즈에서 ST-1 과 ST-2로 나누어지며, 이 ST-1의 TLC, HPLC 및 알루미나 컬럼상의 거동이 파랑비늘돔에서 얻은 ST-1의 그것과 같으므로 scaritoxin으로 보고한 ST-1은 ciguatoxin의 형태인 less polar cigutoxin (LPCTX) 으로 생각된다.

• Advances in Traditional Medicine
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• 제9권4호
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• pp.335-338
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• 2009

Aerial parts of Sida rhombifolia Linn. (Malvaceae) were extracted successively using various solvents and screened for various parameters of antiarthritic activity such as adjuvant-induced arthritis, motor performance, and histopathological study. The ethanol and the aqueous extracts showed potent activity; further these extracts were fractionated by using column chromatography. The fraction ET1 isolated from ethanol extract showed the most potent antiarthritic activity.

• 농업과학연구
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• 제18권2호
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• pp.119-126
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• 1991

치이즈와 대두 지질의 양상을 비교하기 위하여 이들에 함유되어 있는 지질의 단편, 지질의 종류 및 총지질내의 지방산 조성을 column, thin-layer 및 gas chromatography로 분석하여 그결과를 검토하였다. 치이즈 중성지질, 당지질 및 인지질의 함량은 각각 96.2%, 1.1% 및 0.7%로 나타난 반면 대두의 중성지질, 당지질 및 인지질의 함량은 87.5%, 0.5% 및 4.3%로 나타났다. 치이즈의 총지질 및 중성지질의 주요 지질의 종류는 triglyceride, free fatty acid, cholesterol, diglyceride, monoglyceride 및 polar lipid 였으며 당지질과 인지 질내의 주요 지질의 종류는 triglyceride, diglyceride monoglyceride 및 polar lipid 인 반면 대두 지질 단편중의 지질의 종류는 대부분 triglyceride 였고, 일부 diglyceride, monoglyceride 및 polar lipid의 흔적이 나타났다. 치이즈의 총지질에서 나타난 주요 지방산은 C4:0, C6:0, C8:0, C10:0, C12:0, C14:0, C16:0, C16:1, C16:0, C18:1 및 C18:2 였고, 대두의 총지질에서 나타난 주요 지방산은 C18:0, C18:1, C18:2 및 C18:3였다. 치이즈의 총지질에서 소량의 C14:1, C15:0, C17:0 및 C20:0과 대두의 총지질에서 소량의 C4:0, C6:0 및 C10:0 지방산이 검출되었다.

• 한국식품영양과학회지
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• 제29권1호
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• pp.120-127
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• 2000

Deep-fat frying is a common cooking practice. There has been considerable concern regarding the mutagenic and carcinogenic potential of thermally oxidized oils. Studies on deep-fried foods so far have revealed not much on the mutagenicity of the oils in the foods. Therefore, in the present study, it was attempted to investigate the mutagenicity ofthe thermally oxidized safflower oil. Oil was heated in a home-fryer at a temperature of 180$\pm$3$^{\circ}C$ for 48 hours. Oil samples were taken at 0, 8, 16, 24, 32, 40 and 48 hours of heating, respectively. Each sample was used to study the changes in peroxide value (POV), acid value (AV), iodine value (IV), conjugated dienoic acid (CDA) content, %, and fatty acid composition. Another series of samples were fractionated into non-polar and polar fractions by column chromatography. The mutagenicity of the samples taken from the thermally oxidized oils, as well as the non-polar and polar fractions of the thermally oxidized oils, was investigated with the Ames test. The Ames test was carried out with and without metabolic activation. Bacterial tester strains used in the present study were the histidine auxotrophic strains of Salmonella typhimurium TA100, TA1535 and TA102 were used for the detection of base pair mutations, and TA98 and TA1537 for frame shift mutations. Each series of samples was dissolved in tetraphydrofuran (inhibitor-free) and tested at doses ranging from 0.05 to 5 mg/plate. None of the oil samples taken during the 48 hour oxidation period showed any mugagenic activity. This was the case, even after the activaton with 59 mix. Also, none of the polar and non-polar fractions showed any mutagenic activity on all the strains tested.

• Archives of Pharmacal Research
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• 제17권6호
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• pp.458-461
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• 1994

A new high-performance liquid chromatographic method with column switching has been onto a Bondapak phenyl/corsil $(37-50{\;}{\mu}m)$ precolumn and polar plasma components were washed with 0.06 M borate burffer. After valve switching, the concentrated drug were eluted in the back-flush mode and separated on a ${\mu}-Bondapak$ C18 column with acetonitrilke-phosphate buffer as the mobile phae. The method showed excellent precision, accuracy and speed with detection limit of $0.01{\;}{\mug}/ml^{-1}$. Total analysis time per smaple was less than 20 min and the coefficients of variation for intra and inter-assay were less than 5.635. This method has been successfully applied to plasma smaples from eats after oral administration of omeprazole.

• Journal of the korean society of oceanography
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• 제35권1호
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• pp.34-45
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• 2000

Spatial distributions of phytoplankton biomass and nutrients were examined to investigate the magnitude of phytoplankton blooms along the marginal ice zone (MIZ) in the northwestern Weddell Sea during austral summer of 1995. High phytoplankton biomass was associated with the MIZ in the study area. Vertical stability induced by meltwater appears to be the most important factor controlling phytoplankton biomass distribution. Nitrate concentrations are significantly depleted within the upper water column at the phytoplankton biomass maximum. The time required to attain the observed nutrient depletion was calculated from phytoplankton biomass and nitrate depletion, which ranges from 27 to 68 days in transect 4 and from 33 to 145 days in transect 3. Phytoplankton production was also calculated from nitrate depletion and time-scales of nitrate depletion, which varies from 272 to 1752 mg C m$^{-2}$ day$^{-1}$ in transect 4 and from 327 to 2648 mg C m$^{-2}$ day$^{-1}$ in transect 3. In the Southern Ocean where primary productivity shows large temporal and spatial variations, the productivity measurement from nutrient depletion can provide an average rate of primary production during phytoplankton bloom.

• 한국식품영양과학회지
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• 제20권5호
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• pp.473-478
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• 1991

After the glycolipid(GL) and phospolipid (PL) of flint corn during a growth process were fractionated by silicic acid column chromatography (SACC), the GL and PL of polar lipids were separated by thin layer chromatography (TLC)and quantitative by TLC scanner and the fatty acid composition in polar lipids were determined by gas chromatography (GC). Of the GL in polar lipids were separated by TLC, monoglycosyl diacylglycerol (8.3~29.2%) was the major component, and monoglycosyl ceramide, monoglycosyl sterol were also found as minor components. Of the PL, phosphatidyl choline (27.2~29.5%) and phosphatidyl inositol (42.9~79.1%) were the major components, and phosphatidyl ethanolamine and cardiolipin were also persont in the PL. The major fatty acids in the GL were oleic(27.1~37.1%), linoleic (13.2~35.7%) and palmitic (22.5~25.6%) acids. The major fatty acids in the PL were palmitic (46.5~52.3%), heptadecanoic (23.0~25.1%) and oleic(7.2~14.6%) and GL contained a higher percentage of unsa-turated fatty acids, but PL presented that of the saturated fatty acids.

• Ocean and Polar Research
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• 제32권3호
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• pp.321-329
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• 2010

Photosynthetic pigments, nutrients, and hydrographic variables were examined in order to elucidate the spatio-temporal variation of water column structure and its effect on phytoplankton community structure in the western channel of the Korea Strait in fall 2006 and spring 2007. High phytoplankton biomass in the spring was associated with high salinity, implying that nutrients were not supplied by coastal waters or the Yangtze-River Diluted water (YRDW) with low salinity. Expansion of the Korea Strait Bottom Cold Water (KSBCW) and a cold eddy observed during the spring season might enhance the nutrient supply from the subsurface layer to the euphotic zone. Chemotaxonomic examination showed that diatoms accounted for 60-70% of total biomass, followed by dinoflagellates. Nutrient supply by physical phenomena such as the expansion of the KSBCW and the occurrence of a cold eddy appears to be the controlling factors of phytoplankton community composition in the Korea Strait. Further study is needed to elucidate the mechanisms by which the KSBCW is expanded, and its role in phytoplankton dynamics.

• Ocean and Polar Research
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• 제26권2호
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• pp.113-120
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• 2004

The abundance, biomass and distribution of phytoplankton, bacterioplankton and heterotrophic protists in the Bering Sea were investigated from July to August 1999. Chlorophyll a concentrations in the surface waters ranged from 0.16 to $3.79{\mu}g\;l^{-1}$ Nano-phytoplankton were found to constitute from 63 to 98% of the total phytoplankton biomass, and were clearly the dominant primary producers. The biomass of bacterioplankton in the surface layers varied from 1.46 to $20.2{\mu}g\;C\;l^{-1}$ and accounted for 30% of the total phytoplankton biomass. The biomass of bacterioplankton integrated over a depth of 0 to 100m averaged 65.4% of the total phytoplankton biomass. The surface biomass of heterotrophic protists ranged from 1.2 to $27.4{\mu}g\;C\;l^{-1}$, and was within the same order of magnitude as that of bacterioplankton. Of the total biomass of heterotrophic protists in the upper 100m of the water column, 65% was attributed to protists in the nano-size class. The results of this study suggest that bacteria and nano-protists are important components of the planktonic community in the Bering Sea during the summer season. The abundance of bacterioplankton and planktonic protists decreased from the western to northeastern and eastern regions of the Bering Sea. The abundance of these organisms also decreased with depth. The available evidence suggests that variation in the abundance and distribution of these organisms may be affected by water currents and vertical temperature variation in the Bering Sea.

• Journal of the Korean Wood Science and Technology
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• 제30권4호
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• pp.80-86
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• 2002

졸참나무 (Quercus serrata), 맹종죽 (Phyllostachys pubescens) 및 소나무 (Pinus densiflora)로부터 제조한 미정제 초액의 휘발성 성분을 고상(固相) 미량추출(solid phase microextraction; SPME)법을 이용하여 분석하였다. 이들 초액 중의 휘발성화합물 분석을 위하여 극성 (CBP 20) 및 무극성 (CBP 1) 칼람을 사용하였으며, 이로부터 총 264개의 피크를 검출하였다. 이들 성분들 중 주요 화합물은 2-butanone, acetic acid, guaiacol, phenol, 4-ethyl guaiacol, cresol류, 4-ethyl phenol, 그리고 syringol이었다. 무극성 칼람을 사용함에 의하여 7개의 성분, 즉, 1-hydroxy-2-butanone, ethisolide, furfuryl acetate, 1-(2-furanyl)-1-propane, 1,2-dimethoxybenzene, 1,2-dimethoxybenzyl alcohol, phenyl acetate를 새로이 동정할 수 있었다. 이 성분들 중 페놀류가 주성분이었으며, 휘발성 성분의 49~65%를 차지하였다. 죽초액의 경우 페놀류의 비율은 다른 두 목초액보다 낮았다. 그러나 중성화합물류 및 유기산류는 소나무 및 졸참나무로부터 제조한 목초액보다 그 비율이 더 높았다. 따라서 이들 죽초액과 목초액 간의 훈취의 차이는 목초액의 서로 다른 휘발성분의 함유량의 차이에 기인한다고 생각된다.