• 한국식품저장유통학회지
• /
• 제2권1호
• /
• pp.173-183
• /
• 1995

This paper was carried out to investigate changes in chromatograms of polysacctatides and soluble pectins on Sephadex G-50 and non-cellulosic neutral sugars of polysaccharides isolated from cell wall of persimmon fruits treated with polygalacturonase and $\beta$-galactosidase in vitro. The chromatogram pattern of soluble pectins extracted from cell wall treated with $\beta$-galactosidase on Sephacryl S-500 column were similar to those of untreatment, but contents of soluble pectins treated with $\beta$-galactosidase were different from those of untreatment. The patterns of chromatograms In soluble pectins extracted from cell wall treated with polygalacturonase were more complex and lower molecular polymer than those of other cell wall-degrading enzyme treatments. Non-cellulosic neutral sugar of polysaccharides in fraction I of soluble material treated with polygalacturonase was rhamnose, those in fraction II were similar to those in fraction III and contents of arabinose, xylose and glucose were higher than contents of other non-cellulosic neutral sugars. Non-cellulosic neutral sugars of polysaccharides in fraction I in soluble material by $\beta$-galactosidase treatment were rhamnose, arabinose, galactose and mannose. Content of glucose of polysaccharides in fraction II was higher than that in fraction I . Non-cellulosic neutral sugars treated with mixed enzyme were rhamnose, fucose, arabinose, xylose, mannose, galactose and glucose. Compositions of non-cellulosic neutral sugars of polysaccharides in fraction I were similar to those in fraction II and III.

• 한국식품영양과학회지
• /
• 제20권5호
• /
• pp.488-493
• /
• 1991

Lactobacilus acidophilus를 이용한 배추김치제조 연구의 일환으로 이 균이 생성하는 김치의 연화와 밀접한 관련이 있는 효소의 하나인 polygalacturonase(PG)의 성질을 균체내 및 균체외 PG로 나누어 조사하였다. Gel 여과법에 의하여 측정한 균체내외 PG의 개략적인 분자량은 각각 420,000 및 500,000dalton 이었으며, Km값은 다같이 4.0mg/ml이었고, Vmax는 각각 8.0 및 $0.3{\mu}mol$ galacturonic acid/ml/30min 이었다. 최적 pH는 균체내외 PG 다같이 5.5 부근이었으며, 최적온도는 $30^{\circ}C$, 최적 소금농도는 2~3%이었다. 효소활성은 $60^{\circ}C에서\;90^{\circ}C$로 높아질수록 크게 저하하였으며 $60^{\circ}C$에서의 잔존활성은 균체내 PG의 경우 50%, 균체외 PG의 경우 58% 이었고 $90^{\circ}C$에서는 균체내 PG는 5%, 균체외 PG는 19%로서 균체외 효소의 열안정성이 높았다.