• Food Science and Preservation
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• v.2 no.1
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• pp.173-183
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• 1995

This paper was carried out to investigate changes in chromatograms of polysacctatides and soluble pectins on Sephadex G-50 and non-cellulosic neutral sugars of polysaccharides isolated from cell wall of persimmon fruits treated with polygalacturonase and $\beta$-galactosidase in vitro. The chromatogram pattern of soluble pectins extracted from cell wall treated with $\beta$-galactosidase on Sephacryl S-500 column were similar to those of untreatment, but contents of soluble pectins treated with $\beta$-galactosidase were different from those of untreatment. The patterns of chromatograms In soluble pectins extracted from cell wall treated with polygalacturonase were more complex and lower molecular polymer than those of other cell wall-degrading enzyme treatments. Non-cellulosic neutral sugar of polysaccharides in fraction I of soluble material treated with polygalacturonase was rhamnose, those in fraction II were similar to those in fraction III and contents of arabinose, xylose and glucose were higher than contents of other non-cellulosic neutral sugars. Non-cellulosic neutral sugars of polysaccharides in fraction I in soluble material by $\beta$-galactosidase treatment were rhamnose, arabinose, galactose and mannose. Content of glucose of polysaccharides in fraction II was higher than that in fraction I . Non-cellulosic neutral sugars treated with mixed enzyme were rhamnose, fucose, arabinose, xylose, mannose, galactose and glucose. Compositions of non-cellulosic neutral sugars of polysaccharides in fraction I were similar to those in fraction II and III.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.5
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• pp.488-493
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• 1991

The characteristics of endogenous and exogenous polygalacturonase(PG) produced from L. acidophilus were investigated as one of the serial studies on the fermentation of Chinese cabbage kimchi using L. acidophilus. Apparent molecular weigth of endogenous and exogenous PG were estimated to be 420,000 and 500,000 daltons by the method of gel filtration and Vmax of the enzymes 8.0 and $0.3{\mu}mol$ galacturonic acid/ml/30min, respectively and Km of the enzymes were 4.0mg/ml. The optimum temperature, pH and salt concentration of the both enzymes were the same and appeared to $30^{\circ}C$, 5.5 and 2~3%, respectively. The activities of the endogenous and exogenous PG all were severely decreased by increasing of temperature to $90^{\circ}C\;from\;60^{\circ}C$ and its remained activities at $60^{\circ}C\;and\;90^{\circ}C$ were 50%, 58%, and 19%, 5%, respectively.