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Wavelength selection by loading vector analysis in determining total protein in human serum using near-infrared spectroscopy and Partial Least Squares Regression

  • Kim, Yoen-Joo;Yoon, Gil-Won
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.4102-4102
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    • 2001
  • In multivariate analysis, absorbance spectrum is measured over a band of wavelengths. One does not often pay attention to the size of this wavelength band. However, it is desirable that spectrum is measured at only necessary wavelengths as long as the acceptable accuracy of prediction can be met. In this paper, the method of selecting an optimal band of wavelengths based on the loading vector analysis was proposed and applied for determining total protein in human serum using near-infrared transmission spectroscopy and PLSR. Loading vectors in the full spectrum PLSR were used as reference in selecting wavelengths, but only the first loading vector was used since it explains the spectrum best. Absorbance spectra of sera from 97 outpatients were measured at 1530∼1850 nm with an interval of 2 nm. Total protein concentrations of sera were ranged from 5.1 to 7.7 g/㎗. Spectra were measured by Cary 5E spectrophotometer (Varian, Australia). Serum in the 5 mm-pathlength cuvette was put in the sample beam and air in the reference beam. Full spectrum PLSR was applied to determine total protein from sera. Next, the wavelength region of 1672∼1754 nm was selected based on the first loading vector analysis. Standard Error of Cross Validation (SECV) of full spectrum (1530∼l850 nm) PLSR and selected wavelength PLSR (1672∼1754 nm) was respectively 0.28 and 0.27 g/㎗. The prediction accuracy between the two bands was equal. Wavelength selection based on loading vector in PLSR seemed to be simple and robust in comparison to other methods based on correlation plot, regression vector and genetic algorithm. As a reference of wavelength selection for PLSR, the loading vector has the advantage over the correlation plot since the former is based on multivariate model whereas the latter, on univariate model. Wavelength selection by the first loading vector analysis requires shorter computation time than that by genetic algorithm and needs not smoothing.

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Comparative Efficacy of Some Selected Plant Derived Biopesticides for the Control of Insect Pests of Cowpea (Vigna unguiculata (L.) Walp.) in Katsina State, Nigeria

  • Ahmed, B.I.;Onu, I.;Mudi, L.;Aliyu, M.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.52 no.2
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    • pp.183-197
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    • 2007
  • Field trial was conducted at the Research Farm of the Katsina State Agricultural and Rural Development Authority during the farming season of 2004 with a view to evaluate/determine the efficacy of some selected plant-derived biopesticides against the insect pests of cowpea as well as their effect on yield. The variety of the cowpea used was IT86D-719 and the plant derived biopesticides evaluated during the trial were chilli pepper, garlic, ginger, neem, sweetsop and tobacco. The experimental field was ploughed, harrowed and thereafter ridged before the commencement of the 2004 planting season. A total of twenty one (21) experimental plots were demarcated and arranged into seven treatment plots. The size of each plot was $5m{\times}4m$ while interspaces between adjacent plot and blocks were 1m and 2m, respectively. Results of the experiments showed that all the plant-derived biopesticide treatments were significantly (p>0.05) better than control treatment. The order of effectiveness of the treatments was tobacco (80-90%), sweetsop (75-85%), garlic (70-80), neem (72-78%), chilli pepper (60-70%) and ginger (30-50%). Furthermore, yield result corresponded positively with the effectiveness of the treatments. Results of the present finding suggest the use of tobacco, sweetsop and garlic as promising biopesticides in the control of cowpea insect pests.

English Bible Text Visualization Using Word Clouds and Dynamic Graphics Technology (단어 구름과 동적 그래픽스 기법을 이용한 영어성경 텍스트 시각화)

  • Jang, Dae-Heung
    • The Korean Journal of Applied Statistics
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    • v.27 no.3
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    • pp.373-386
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    • 2014
  • A word cloud is a visualization of word frequency in a given text. The importance of each word is shown in font size or color. This plot is useful for quickly perceiving the most prominent words and for locating a word alphabetically to determine its relative prominence. With dynamic graphics, we can find the changing pattern of prominent words and their frequencies according to the changing selection of chapters in a given text. We can define the word frequency matrix. In this matrix, rows are chapters in text and columns are ranks corresponding to word frequency about the words in the text. We can draw the word frequency matrix plot with this matrix. Dynamic graphic can indicate the changing pattern of the word frequency matrix according to the changing selection of the range of ranks of words. We execute an English Bible text visualization using word clouds and dynamic graphics technology.

The Effect of Stream Anion and River-Bed Materialson Aquatic Insects (계류수의 음이온과 하상재료가 수서곤충에 미치는 영향)

  • Seo, Mun Won;Chun, Kun-Woo
    • Journal of Forest and Environmental Science
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    • v.15 no.1
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    • pp.89-97
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    • 1999
  • This study was carried out to obtain basic data on the kinds of aquatic insects and their living conditions in the mountain stream. The investigation was done in Bongmyung stream. Experimental Forest, Kangwon National University on aquatic insects, anions and river-bed materials. The results are as follows. 1. At every plot surveyed, diversity index, richness index and evenness index of aquatic insects appeared higher at upper stream than at lower stream in erosion control dam. 2. Anion concentrations were almost the same in plots A, B, C, D and E, but plot F at the lower stream showed 1.5 to 89 times higher concentration than the others. 3. In river-bed materials analysed, particle diameter was bigger at the upper stream than at the lower stream. At the down stream of erosion control dam showed high pebble composition ratio. 4. The number of aquatic insects showed the negative relation with the anion concentration and the positive one with the size of river-bed materials. Especially, they were affected much by the distribution chart of boulder.

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Forest Resources of the Korea Based on National Forest Inventory Data

  • Kim, Dong-Hyuk;Nor, Dae-Kyun;Jeong, Jin-Hyun;Kim, Sung-Ho;Chung, Dong-Jun
    • Journal of Forest and Environmental Science
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    • v.24 no.3
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    • pp.159-164
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    • 2008
  • Forest inventory is a commercial term meaning the preparation of detailed descriptive list of articles with number, quantity and value of each item included. Forest inventory deals with the measurement of trees and stands, the estimation of their volume, growth prediction, biomass, carbon stocks and the description tree characteristics, as well as the land upon which they are growing. National Forest Inventory Center (NFIC) in Korea conducts national forest inventory every 5 years to obtain accurate baseline data for national forest policy. The permanent sample plot data used in were collected by NFI. The objective of this study was to develop methods for quantifying forest resources at national scale based on $5^{th}$ National Forest Inventory (NFI) data in Korea. Forest land area decreased from 6.44 to 6.38 million ha between 1997 and 2007, continuing a slight downward trend in area beginning in the late 1990s. However forest resources of the Korea have continued improving in general condition and quality, as measured by increased average size and volume of trees. Growing-stock volume of the Korea increased from 17 to 123.79 cubic meter per ha between 1976 and 2007. The biomass in Korea was estimated to be 153.81 tons per hectare and carbon stocks in Korea was estimated to be 84.36 tons per hectare by NFI data. This information is important for government officials, public administration, the private business sector, and the researcher. Forest Inventory should be implemented in a way to be able to monitor and assess the forests continuously.

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The accuracy of linear measurements of maxillary and mandibular edentulous sites in conebeam computed tomography images with different fields of view and voxel sizes under simulated clinical conditions

  • Ganguly, Rumpa;Ramesh, Aruna;Pagni, Sarah
    • Imaging Science in Dentistry
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    • v.46 no.2
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    • pp.93-101
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    • 2016
  • Purpose: The objective of this study was to investigate the effect of varying resolutions of cone-beam computed tomography images on the accuracy of linear measurements of edentulous areas in human cadaver heads. Intact cadaver heads were used to simulate a clinical situation. Materials and Methods: Fiduciary markers were placed in the edentulous areas of 4 intact embalmed cadaver heads. The heads were scanned with two different CBCT units using a large field of view ($13cm{\times}16cm$) and small field of view ($5cm{\times}8cm$) at varying voxel sizes (0.3 mm, 0.2 mm, and 0.16 mm). The ground truth was established with digital caliper measurements. The imaging measurements were then compared with caliper measurements to determine accuracy. Results: The Wilcoxon signed rank test revealed no statistically significant difference between the medians of the physical measurements obtained with calipers and the medians of the CBCT measurements. A comparison of accuracy among the different imaging protocols revealed no significant differences as determined by the Friedman test. The intraclass correlation coefficient was 0.961, indicating excellent reproducibility. Inter-observer variability was determined graphically with a Bland-Altman plot and by calculating the intraclass correlation coefficient. The Bland-Altman plot indicated very good reproducibility for smaller measurements but larger discrepancies with larger measurements. Conclusion: The CBCT-based linear measurements in the edentulous sites using different voxel sizes and FOVs are accurate compared with the direct caliper measurements of these sites. Higher resolution CBCT images with smaller voxel size did not result in greater accuracy of the linear measurements.

The Structural Characterization of Recombinant Bovine Somatotropin Expressed in Escherichia coli (재조합 소성장호르몬의 구조적 특성)

  • 김정호;김훈주박은숙김준
    • KSBB Journal
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    • v.9 no.2
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    • pp.165-173
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    • 1994
  • In this paper we have described the structural characterization of recombinant bovine somatotropin produced in Escherichia coli. Recombinant bovine somatotropin consists of 191 amino acid residues with a calculated molecular weight of 21,802 Da. For fragmentation of recombinant bovine somatotropin, we have used trypsin, Staphylococcus aureus V8 pretease, CNBr, and mild acid hydrolysis method. Digestion and cleavage with these proteases and chemicals yielded peptides of various size for amino acid sequence determination. The N-terminal sequence analysis was carried out up to thirty residues. Because the design of the recombinant bovine somatotropin gene for expression was such that the coding sequence begins with an initiation codon, AUG, before Ala, the first amino acid of bovine somatotropin, we could expect the initial amino acid as N-formyl Met. But the first amino acid of this protein, expressed in E. coli cells as inclusion bodies, was Ala. And the amino acid composition of RP-HPLC purified recombinant bovine somatotropin was determined and no essencial difference was observed. The amino acid sequence of the recombinant bovine somatotropin was identical to that predicted from its recombinant gene. There was no processing or replacement of amino acid residues in recombinant bovine somatotropin expressed in E. coli. The hydropathy plot of recombinant bovine somatotropin revealed a hydrophobic region at the NH2-terminus and hydrophilic region at the COOH-terminus. The E. coli expression system is thought to be valuable for the expression of recombinant bovine somatotropin because protein was processed to remove the N-terminal Met residue by methionyl-aminopeptidase autonomously.

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Studies on the Structure of the Forest Community in Mt. Sokri(II) -Analysis on the Plant Community by the Classification and Ordination Techniques- (속리산 삼림군집구조에 관한 연구(II) Classification 및 Ordination 방법에 의한 식생분석 -)

  • 이경재;박인협;조재창;오충현
    • Korean Journal of Environment and Ecology
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    • v.4 no.1
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    • pp.33-43
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    • 1990
  • A survey of Popju Temple district. was conducted using 70 sample plots of 500$m^2$ size. The classification by TWINSPAN and DCA ordination were applied to the study area in order to classify them into several groups based on woody plants and environmental variables. By both techniques. the plant com-munity were divided into six groups by the altitude and soil moisture. The successional trends of tree species seem to be from Pinus densiflora, Sorbus alnifolia through Quercus serrata to Carpinus laxiflora and from P. densiflora, Fraxinus sieboldiana through Q. mongolica in the canopy layer, and from Lespedeza cyrtobotrya, Rhus trichocarpa, Zanthoxylum schnifolium through Rhododendron mucronulatum, Corylus sieboldiana, Lindera obtusiloba, Magnclia sieboldii to Euonymus sieboldianus in the understory and shrub layer. The species diversity of the plant community in the burnt plot was decreased by the forest fire but the importance values of Quercus species were increased in above plot.

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The Presence and Role of Intergranular Phase in Nd8Fe86-xNbxB6 (x = 0, 1, 2, 3) Nanocomposite Magnet Characterized by Mossbauer Spectroscopy

  • Han, Jong-Soo;Yang, Choong-Jin;Park, Eon-Byeung;Kim, Eng-Chan
    • Journal of Magnetics
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    • v.10 no.1
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    • pp.14-22
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    • 2005
  • Precisely refined Mossbauer study and nano structure observation revealed that intergranular phase formed between a-Fe and Nd₂Fe14B phase in NdFeNbB alloys plays a significant role on the magnetic properties. The intergranular interaction was characterized in term of Henkel Plot (δM plot), and hyperfine field, quardrupole splitting and isomer shift were refined to predict the presence and role of the intergranular phase. By the addition of Nb into Nd8Fe86B6 composition, coercivity was found to increase by 25% due to the refinement of average grain size of both the soft and hard magnetic phases which was decreased from 50 nm of virgin Nd/sub 8/Fe/sub 86/B/sub 6/ to 25 nm in Nd8Fe 85Nb₁B6 alloys. The role of Nb addition was confirmed to stabilize the Nd₂Fe14B lattice preventing from thermal vibration of the corresponding sites substituted Fe by Nb atoms in all sites in the Nd₂Fe14B lattice. The enhanced coercivity was originated from the exchange hardening of soft and amorphous phases surrounding the hard magnetic Nd₂Fe14B crystal.

A Study for Quality Improvement of Three-dimensional Body Measurement Data (3차원 인체치수 조사 자료의 품질 개선을 위한 연구)

  • Park, Sun-Mi;Nam, Yun-Ja;Park, Jin-Woo
    • Journal of the Ergonomics Society of Korea
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    • v.28 no.4
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    • pp.117-124
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    • 2009
  • To inspect the quality of data collected from a large-scale body measurement and investigation project, it is necessary to establish a proper data editing process. The three-dimensional body measurement may have measuring errors caused from measurer's proficiency or changes in the subject's posture. And it may also have errors caused in the process of algorithm expressing the information obtained from the three-dimensional scanner into numerical values, and in the course of data-processing dealing with numerous data for individuals. When those errors are found, the quality of the measured data is deteriorated, and they consequently reduce the quality of statistics which was conducted on the basis of it. Therefore this study intends to suggest a new way to improve the quality of the data collected from the three-dimensional body measurement by proposing a working procedure identifying data errors and correcting them from the whole data processing procedure-collecting, processing, and analyzing- of the 2004 Size Korea Three-dimensional Body Measurement Project. This study was carried out into three stages: Firstly, we detected erroneous data by examining of logical relations among variables under each edit rule. Secondly, we detected suspicious data through independent examination of individual variable value by sex and age. Finally, we examined scatter-plot matrix of many variables to consider the relationships among them. This simple graphical tool helps us to find out whether some suspicious data exist in the data set or not. As a result of this study, we detected some erroneous data included in the raw data. We figured out that the main errors are not because of the system errors that the three-dimensional body measurement system has but because of the subject's original three-dimensional shape data. Therefore by correcting some erroneous data, we have enhanced data quality.