• Animal Bioscience
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• v.36 no.1
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• pp.144-155
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• 2023

Objective: Adipocyte differentiation is regulated by a variety of functional genes and noncoding RNAs. However, the role of miRNAs in lipid deposition of goat white adipose tissue is still unclear. Therefore, this study revealed the miRNA expression profile in goat subcutaneous adipocytes by sRNA-seq. Methods: The miRNA expressed in goat subcutaneous preadipocytes and the mature adipocytes were sequenced by sRNA-seq. The differentially expressed miRNAs (DEm) were screened and gene ontology (GO) and Kyoto encyclopedia for genes and genomes (KEGG) analyses were performed. Gain-of-function and loss-of-function combined with oil red O staining, Bodipy staining, and quantitative reverse-transcription polymerase chain reaction (qPCR) were utilized to determine the effect of miR-133a-3p on adipocyte differentiation. Results: A total of 218 DEm were screened out. The target genes of these DEm were significantly enriched in GO items such as biological regulation and in KEGG terms such as FAK signaling pathway and MAPK signaling pathway. qPCR verified that the expression trend of miRNA was consistent with miRNA-seq. The gain-of-function or loss-of-function of miR-133a-3p showed that it promoted or inhibited the accumulation of lipid droplets, and CCAAT enhancer binding protein α (C/EBPα) and C/EBPβ were extremely significantly up-regulated or down-regulated respectively (p<0.01), the loss-of-function also led to a significant down-regulation of peroxisome proliferator activated receptor gamma (PPARγ) (p<0.01). Conclusion: This study successfully identified miRNAs expression patterns in goat subcutaneous adipocytes, and functional identification indicates that miR-133a-3p is a positive regulator of the differentiation process of goat subcutaneous adipocytes. Our results lay the foundation for the molecular mechanism of lipid deposition in meat-source goats from the perspective of miRNA.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.737-740
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• 2005

Soil moisture, through its dominance in the exchange of energy and moisture between the land and atmosphere, plays a crucial role in influencing atmospheric circulation. To identify the crucial role, it is a common agreement that knowledge of land surface processes and development of remote sensing techniques are of great important scientific issues. This research uses TRMM satellite C band (10.65 GHz) data to retrieve soil moisture on the Tibetan Plateau in Mainland China. Two retrieval schemes that are implemented include the t-(J) model and the R model. The latter one is developed based on a land surface process and radiobrightness (R) model for bare soil and vegetated terrain. Compared with the in situ ground measurements, the soil moisture retrieved from the R model and the t-(J) model with vegetation information obviously appear more accurate than that derived from bare soil model. Retrieved soil moisture contents from the two inversion models, R model and t-(J) model, have a similar trend, but the former appears to be superior in terms of correlation coefficient and bias compared with in situ data. In the future, we will apply the R model with the TRMM 10.65 GHz brightness temperature to monitor long-term soil moisture variation over Tibet Plateau.

• Journal of Veterinary Clinics
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• v.34 no.5
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• pp.366-369
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• 2017

A 30 kg, 6-year-old spayed female Samoyed dog was referred with a history of intermittent weight-bearing lameness in her right hindlimb for 3 weeks. The patient hadsurgery to correct a medial patellar luxation in the same limb 3 years prior. Based on the physical examination and radiographic findings, MPL and CCLR of the right hindlimb were diagnosed. Pre-surgical arthroscopy examination was performed, revealing a complete rupture of the cranial cruciate ligament, medial caudal meniscal tears and fibrotic cartilagechanges on the trochlear groove. An arthroscopy-assisted partial meniscectomy was used to repair themedial caudal meniscus. To correct the tibial plateau angle and medial patellar luxation, a tibial plateau leveling osteotomy (TPLO) was performed. A tibial tuberosity transposition (TTT) was performed to realignthe quadriceps mechanism with the trochlear block recession followed by soft tissue reconstruction. The post-surgical recovery was uneventful, and the patient was weight-bearing with normal ambulation on the repaired limb. There were no complications, and the implants were well positioned at the last follow-up. The clinical outcome of the caseindicates that combining TPLO with TTTis a good surgical option for treatingconcurrent CCLR and MPL.

• Journal of the Korean Arthroscopy Society
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• v.1 no.1
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• pp.102-107
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• 1997

Discoid meniscus has been well known to a frequent anomaly of meniscus and much reported in diagnosis and treatment. But, associated X-ray findings have not been known well. We reported its significance of X-ray findings in discoid meniscus. From May 1995 to May 1997, 31 cases of discoid meniscus were compared with 51 cases of nondiseoid meniscus both confirmed by arthroscopy, with simple X-my findings. Both groups were evaluated by X-ray findings in view of lateral joint space widening, peripheral spur of lateral joint space, sclerotic change of lateral tibial plateau, concave lateral tibial plateau, flattening of lateral femoral condyle, hypoplasia of lateral femoral condyle and high riding fibula. The results of analysis were as follows: 1. Widening of lateral joint space, sclerotic change of lateral tibial plateau and cupping of lateral tibial plateau were statistically significant in discoid meniscus. 2. X-ray findings in discoid meniscus were not associated with patient's injury history and symptom duration. 3. Other X-ray findings were not related in discoid meniscus significantly. X-ray findings with patient's history and physical examination arc helpful in the diagnosis of discoid meniscus.

• Journal of the Korean Chemical Society
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• v.29 no.6
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• pp.646-650
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• 1985

RNA degradation by riboinuclease A (EC 3.1.27.5) was inhibited by spermine. As the concentration of spermine was increased, the ribonuclease activity was decreased gradually until it reached a plateau. Under the same conditions, the viscosity of the RNA increased, as the spermine concentration was increased until it reached a plateau in the same manner as the profile of the spermine-dependent ribonuclease activity. The inhibitory effect of spermine on the susceptibility of RNA to the ribonuclease could be relieved by denatured calf thymus DNA, but not by the native DNA. The data here indicate the possibility that the suppress of the RNA susceptibility for the ribonuclease by spermine is brought about by the spermine-induced intermolecular aggregation of the RNA molecules.

• Journal of Korean Academy of Nursing Administration
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• v.21 no.5
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• pp.530-541
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• 2015

Purpose: This study was designed to construct a structural equation model and identify the effects and relationships of self-efficacy, career plateau, job embeddedness, organizational commitment and intent to turnover for nurses. Also, an attempt was made to derive a hypothetical model from these factors and to verify whether the model has validity as a means of explaining and anticipating turnover intention of nurses. Methods: Data were collected from six randomly selected hospitals: a university hospital located in J city, Kyungnam and five general hospitals located in two cities (cities B and C), each having 400 to 720 beds. Also, 318 nurses were surveyed. Data were analyzed using SPSS 21.0 and AMOS 18.0 program. Results: Job embeddedness and organizational commitment were identified as the most likely paths to turn over intention. Also, organizational commitment had the highest direct effect on turnover intention followed by job embeddedness and then self-efficacy, but career plateau had an indirectly effected turnover intention. Conclusion: As turnover intention was found to be greatly affected by organizational commitment and job embeddedness, methods to improve organizational commitment and job embeddedness should be actively developed to reduce turnover intention among nurses.

• Korean Journal of Metals and Materials
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• v.50 no.10
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• pp.703-710
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• 2012

The effects of the ordering reaction on high temperature mechanical behavior is investigated by tensile tests at $2{\times}10^{-2}/s-3.3{\times}10^{-5}/s$ up to $745^{\circ}C$. The tensile deformed region is examined by differential scanning calorimeter (DSC), TEM, and high resolution neutron diffraction (HRPD). The results showed that a plateau of tensile strength appeared at $150-500^{\circ}C$ whereas the elongation minimum occurred at about $600^{\circ}C$. This suggests that the occurrence of a plateau does not cause the elongation minimum. The temperature of the elongation minimum decreases with the strain rate. HRPD results show a lattice contraction in the tensile deformed specimen at the temperature of the plateau occurring region. The plateau of tensile strength, the lattice contraction, and the occurrence of serration appeared in the same temperature region.

• Parasites, Hosts and Diseases
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• v.59 no.3
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• pp.311-317
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• 2021

The present study reports a rare case of Taenia saginata infection, which was initially diagnosed as acute cholecystitis in a Tibetan patient at the Qinghai-Tibetan Plateau pastoral area, China. A 45-year-old female was initially diagnosed with acute cholecystitis at a hospital in China. She had a slight fever, weight loss and constipation and complained of pain in the upper abdomen and left back areas. Increase of monocyte, eosinophil and basophil levels were shown. Taenia sp. eggs were detected in a fecal examination. An adult tapeworm approximately 146 cm in length, whitish-yellow color, was collected from the patient after treatment with traditional Chinese medicine. The adult tapeworm had a scolex and proglottids with genital pores. The scolex was rectangular shape with 4 suckers and rostellum without hooklet. The cox1 gene sequence shared 99.5-99.8% homology with that of T. saginata from other regions in China. The patient was diagnosed finally infected with T. saginata by morphological and molecular charateristics.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.10
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• pp.5078-5090
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• 2013

This study was to identify the relationship among small-medium sized hospitals nurse's career plateau, self-efficacy, job embeddedness and turnover intention. The study was conducted through a survey on 217 nurses in five small-medium sized hospitals in J city. The data were collected from August 5, 2013 to August 20, 2013. The data were analyzed by SPSS/WIN 18.0 program. The mean score of turnover intention was 3.07. The positive correlations between career plateau and turnover intention were found, but the negative correlation between self-efficacy, job embeddedness and turnover intention were found. The significant factors influencing turnover intention were job embeddedness, present clinical career, career plateau and work unit. To decrease turnover intention, therefore, nursing managers need to develop the nurse retention program for increasing job embeddedness and apply to nursing field.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.3
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• pp.309-314
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• 2006

The present investigation was undertaken to study the milkability characteristics of Holstein cows. Out of 6,660 initial records 4,607 records were finally included in the analysis after deleting some records with an abnormal range of values for the traits considered. These 4,607 Holstein cows belonged to a total of 122 herds located in a province in Korea. The 'LactoCorder' instrument was used to electronically record the milkability traits at the milking parlor. A total of 19 traits were studied which were broadly classified into milk yield, milking speed, milking time and the electrical conductivity related traits. The SAS 9.1 statistical software was used to carry out analyses. The average maximum milk flow per minute was 3.21 kg/min, while the average milking speed during the main milking process (DMHG) had a mean value of 2.30 kg/min. The total milk yield was 14.14 kg, 62% of which was milked during the first three minutes of the milking. The average total milking time was 8.23 min. Among the three phases of the main milking process, the time of stable milk flow had the longest time (2.97 min) followed by the time at the decline phase (2.62 min). The average time taken to reach the plateau phase was 1.08 min, which can still be reduced further through improved managemental practices. Among milk yield traits, milk yield during the first two (MG2) and three (MG3) minutes of milking had high positive correlation with milking speed traits and negative correlations with almost all the milking time traits except time of incline in milk flow from 0.5 kg/min till the attainment of the plateau phase (tAN). Milking speed traits had negative correlations with total milking time, time at main milking process, time at plateau and with the time at decline. Since there was medium to high negative correlation between the milking speed and the time at plateau, there is a need for selection of cows which have intermediate milking speed so that it could require less milking time and also the optimum (higher and longer) plateau time. Proper pre-stimulation and avoiding over milking will help in further reducing the milking time and thus will add to the net profit of the farmer.