• Tuberculosis and Respiratory Diseases
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• 제78권4호
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• pp.396-400
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• 2015

IgG4-related disease is an immune-mediated fibro-inflammatory disease, characterized by lymphoplasmacytic infiltration composed of IgG4-positive plasma cells of various organs with elevated circulating levels of IgG4. This disease is now reported with increasing frequency and usually affects middle-aged men. Massive pleural effusion in children is an uncommon feature in IgG4-related disease. Here, we report a case of a 16-year-old male patient with extensive IgG4-related disease presenting with massive pleural effusion, mediastinal mass, and mesenteric lymphadenopathy.

• Journal of Nutrition and Health
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• 제33권6호
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• pp.597-612
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• 2000

The aim of this study was to evaluate the effects of soyoligosaccharides on intestinal microflora transit time lipid profiles and immune responses. Forty-eight male rats of Sprague-Dawley strain were divided into six groups(SYS: soybean oil-sucrose SYO: soybean oil-oligo SES; esame oil-sucrose SEO:sesame oil-oligo BFS : beef tallow-sucrose BFO : beef tallow-oligo) soyoligo-groups(SYO, SEO, BEO) were fed the diet containing 5% soyoligosaccharides but control groups not fed them and all groups were fed 20% fats for 4 weeks. The number of fecal bifidobacteria tended to increase after soyoligosaccharides feeding. The significant increase was found in SEO group compared to SES in that of fecal bifidobacteria,. Stool wet weight dry fecal weight and stool water content were increased significantly in soyoligo-group(SYO, SEO, BFO) compared to control groups(SYS, SES, BFS) and gastrointestinal transit time(TT) tended to increase. Soyoligosaccharides lowered plasma cholesterol in SEO group significantly more than did other groups. soyoligo-groups(SYO, SEO, BFO) tended to decrease liver triglycerides compared to control groups. Plasma IgG concentration increased in beef tallow groups(BFS, BFO) than that in other dietary fat groups. Soyoligosaccharides and kinds of fats had effects on plasma C3 concentration and mitogenesis of the spleen cells. Soyoligosaccharides had no effects on plasma leptin and insulin concentration but kinds of fats did so that sesame groups were decreased significantly in plasma leptin concentration but increased significantly in plasma insulin concentration compared to other dietary fat groups. In conclusion dietary soyoligosaccharides changed the composition of the intestinal microflora beneficially with sesame oil and partially had effect on plasma and liver lipid profiles .(Korean J Nutrition 33(6) : 597∼612, 2000)

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제4권1호
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• pp.10-17
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• 2001

목 적: 성인의 경우 사회경제적 수준과 위생상태, 직업, 주거환경 등이 H. pylori 감염과 관련된다고 보고되고 있으나, 소아를 대상으로 한 H. pylori 감염에 대한 역학조사는 비교적 드물다. 본 소아과학교실에서는 H. pylori 감염에 영향을 주는 생활환경 요인을 알아보고자 신촌지역 초등학교 학생들을 대상으로 하여 생활환경과 H. pylori 양성률을 조사하였다. 방 법: 신촌지역 초등학생 571명을 대상으로 하여 H. pylori IgG 항체 양성유무와 생활환경에 대한 설문조사를 시행하였다. H. pylori IgG 항체가는 GAP IgG Helicobacter pylori kit (Bio-Rad Lab, USA)를 이용하여 ELISA법으로 측정하였고, 항체 가가 15 U/mL 이상이면 양성, 15 U/mL 미만인 경우 음성으로 판정하였다. 생활환경 조사는 설문지를 이용하였고, 출생지와 월수익, 주거환경, 가족수, 형제수, 소화성궤양 및 위암의 가족력 등을 조사하였다. 결 과: 1) 대상환아는 총 571명으로 평균 연령은 $9.6{\pm}1.8$ (6~13.6)세였고, 남녀비는 1.1：1이었다. 전체 환아의 H. pylori IgG 항체 양성률은 16.8% (96/571)였다. 연령별 H. pylori IgG 항체 양성률은 6세에서 10.4%, 7세 17.9%, 8세 11.1%, 9세 19.3%, 10세 16.5%, 11세 13.7%, 12세 이상에서 30.9%였으며(p=0.06), 성별 H. pylori IgG 항체 양성률은 남학생에서 16%, 여학생 18%였다(p=0.46). 2) 출생지별 H. pylori IgG 항체 양성률은 호남 8% (1/13), 서울 17% (66/381), 충청도 20% (1/5), 영남 20% (1/5), 경기도 24% (7/29), 강원도 75% (3/4)였다(p>0.05). 3) 소득수준별 H. pylori IgG 항체 양성률은 월소득 100만원 이하에서는 17%(10/58), 월소득 100~200만원에서는 16% (44/274), 월소득 200~500만원에서는 19% (22/117), 월소득 500만원 이상에서는 17% (2/12)였다(p=0.93). 4) 주거환경별 H. pylori IgG 항체 양성률은 아파트의 경우 25% (3/15), 단독주택의 경우 18% (64/358), 기타 17% (21/124)였다(p>0.05). 5) 가족수에 따른 H. pylori IgG 항체 양성률은 2명 이하 13% (10/79), 3명 15% (37/250), 4명 19% (18/96), 5명 이상 30% (17/57)로 가족수가 증가할수록 H. pylori IgG 항체 양성률이 유의하게 높았다 (p=0.03). 형제수별로는 1명인 경우 16% (68/420), 2명인 경우 18% (10/55), 3명 이상인 경우 33% (5/15)였다(p=0.27). 6) 위암 및 소화성궤양의 가족력 유무별 H. pylori IgG 항체 양성률은 통계학적으로 유의한 차이가 없었다(p>0.05). 7) 부모의 직업별 H. pylori IgG 항체 양성률은 통계학적으로 유의한 차이가 없었다(p>0.05) 8) 연령, 소득수준, 주거환경, 가족구성원 수, 위암 및 소화성궤양의 가족력에 따른 로지스틱 회귀분석을 시행한 결과 H. pylori IgG 항체 양성률과 가장 깊은 관계를 갖는 생활요인은 가족수의 증가였으며, 비교위험도는 1.5였다(p=0.003). 결 론: 서울 지역 초등학생의 H. pylori 양성률은 연령에 비례하여 증가하는 양상을 보였으며 평균 감염률은 16.7%였고, H. pylori 감염률에 영향을 미치는 유일한 생활환경적 요인은 가족 구성원의 수였으며 odds ratio는 1.5이었다. 우리 나라와 같이 비교적 대가족제도가 많은 생활환경에서는 가족구성원의 수가 H. pylori 감염률을 증가시키는 요인의 하나가 될 것으로 생각된다.

• 대한한의학회지
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• 제32권3호
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• pp.25-34
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• 2011

Objectives: We performed simultaneous determination of five constituents by HPLC in Samul-tang (SMT). Additionally, we investigated the immune-stimulatory potential of SMT on specific cellular and humoral immune responses in ovalbumin (OVA)-immunized mice. Methods: Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array (PDA) detection at 190-400 nm, were used for quantification of the five components of SMT. Mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. C57BL/6 mice were immunized intraperitoneally with OVA/alum ($100{\mu}g/200{\mu}g$) on days 1, 8, and 15. The extract of SMT (1000 mg/kg) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma. Results: Calibration curves were acquired with $r^2$>0.9999, and the relative standard deviation (RSD, %) for intra- and inter-day precision were both less than 3.5%. The recovery was in the range of 95.69-115.12%, with an RSD less than 6.0%. The contents of five components in SMT were 1.08-15.30 mg/g. SMT significantly enhanced Con A-induced splenocyte proliferation in OVA-immunized mice (p<0.01). Also, SMT significantly enhanced OVAspecific IgG, IgG1 and total IgM levels in plasma compared with the OVA-immunized group. Conclusions: The established method will be applied for the quantification of major components and immunestimulating activity in OVA-immunized mouse model of SMT.

• Asian-Australasian Journal of Animal Sciences
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• 제14권8호
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• pp.1157-1163
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• 2001

First-litter gilts were used to determine how different dietary treatments during gestation affect the reproductive performance of gilts and immunity development of their piglets. Twenty-two crossbred $Landrace{\times}Yorkshire$ gilts were randomly assigned to three dietary treatments, Following conception, the gilts were fed experimental diets until farrowing occurred. The diet for treatment I was low protein diet (8% CP), treatment 2 had an additional supplement of 0.14% threonine that was added to the low protein diet, and treatment 3 was a control diet containing 12% CP. During gestation, net body weight gain of sows in treatment group 2 was higher than in treatment group 1 (p=0,075), However, during lactation there was no difference between all treatments groups on body weight loss and their live piglets at birth. Although milk IgG between treatments did not differ, treatment groups 2 and 3 were slightly higher than treatment group I was, Plasma IgG concentrations in piglets were however equal within all treatment groups at birth and at 7 days of age, at 21 days of age, it was higher in treatment group I than it was in the other two groups (p<0.01), Threonine supplementation to a low protein diet during gestation slightly increases milk IgG of sows, It is beneficial for piglets to acquire more passive immunity, but a suppressive effect was also noted on the endogenous IgG synthesis in piglets. A gestation diet of 8% CP for gilts can stimulate immuno-system of her piglets.

• 동의생리병리학회지
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• 제19권2호
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• pp.398-406
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• 2005

This study was performed to investigate the effect of oral administration of GSRE against the asthma. Asthma was induced to Balb/c mouse by i.p. injection and aerosol immunization with ovalbumin. It was observed the change of the eosinophil number in the BALF. Concentrations of IL-4, IL-5 in BALF and splenocyte were assessed by ELISA, IgG and IgE from serum were calculated by same method. Concentration of IL-4 in splenocyte was significantly decreased in GSRE group compared with control group. Concentrations of IL-5 from BALF and splenocyte were significantly decreased in GSRE group compared with control group, respectively. Level of IgE in serum was significantly decreased in GSRE group compared with control group, but not IgG. We found that the effect of GSRE extract in asthma was implicated in reductions of IL-4, IL-5 released from Th2 cell, and decreses of IgE, from plasma cell. These findings suggest that GSRE extract can produce anti-asthmatic effect, which may play a role in allergen-induced asthma therapy.

• 동의생리병리학회지
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• 제24권4호
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• pp.616-623
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• 2010

Three herbal formulas (Bangpungtongsung-san, Ohyaksungi-san, and Ojeok-san) for wind-cold and heat pain symptom were applied to investigate the immunological activities on antigen (Ag)-specific or Ag-non-specific immune responses in murine macrophage cell line (RAW 264.7) and ovalbumin (OVA)-immunized mice. This study was carried out in nitric oxide (NO) synthesis in RAW 264.7 cells and cellular proliferation in mouse splenocytes according to three herbal formulas. C57BL/6 mice were immunized intraperitonially with OVA/aluminium ($100\;{\mu}g/200\;{\mu}g$/mouse) on day 1, 8, and 15. Three herbal formulas were administrated to mice orally for 3 weeks from day 1. On day 22, OVA-, lipopolysaccharide (LPS)-, and concanavalin A (Con A)-stimulated splenocyte proliferation and antibodies (OVA-specific antibodies of the IgG, lgG1, and total IgM classes) in plasma were measured. Ohyaksungi-san increased NO synthesis in RAW 264.7 cells. Ojeok-san and Ohyaksungi-san significantly enhanced cellular proliferation by LPS and Con A in splenocytes from OVA-immunized mice (p<0.001). Three herbal formulas for wind-cold and heat pain symptom also significantly enhanced plasma OVA-specific IgG, IgG1, and total IgM levels compared with the OVA/Alum group. These results suggested that three herbal formulas for wind-cold and heat pain symptom could be used as stimulator of immune response.

• 대한수의학회지
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• 제56권3호
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• pp.167-176
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• 2016

We investigated whether maternal antibodies (mAbs) elicited by dams immunized with recombinant vaccine candidates against avian pathogenic Escherichia coli (APEC) can passively confer protective immunity to chicks. In the present study, pBP244 plasmids carrying selected antigens of APEC were transformed into Salmonella Typhimurium JOL912, which was used as a vaccine candidate against APEC. The hens were immunized with the vaccine candidates using prime or booster doses. The levels of IgG and sIgA specific to the selected antigens increased significantly following prime immunization. To evaluate the persistence of passively transferred mAbs, the levels of IgY and IgA were determined in egg yolks and whites, respectively. The eggs from the immunized group showed consistently increased levels of IgY and IgA until week 16 post-laying (PL) and week 8 PL, respectively, relative to the control group. The presence of mAbs was observed in chicks that hatched from the hens, and titers of plasma IgY were consistently raised in those from the immunized hens by day 14 post-hatching. Further, chicks from the immunized hens were protected from challenge with a virulent APEC strain, whereas those from non-immunized hens showed acute mortality.

• Tuberculosis and Respiratory Diseases
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• 제76권1호
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• pp.38-41
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• 2014

Immunoglobulin G4 (IgG4)-related disease is a newly recognized condition characterized by fibroinflammatory lesions with dense lymphoplasmacytic infiltration, storiform-type fibrosis and obliterative phlebitis. The pathogenesis is not fully understood but multiple immune-mediated mechanisms are believed to contribute. This rare disease can involve various organs and pleural involvement is even rarer. We report a case of IgG4-related disease involving pleura. A 66-year-old man presented with cough and sputum production for a week. Chest radiography revealed consolidation and a pleural mass at right hemithorax. Treatment with antibiotics resolved the consolidation and respiratory symptoms disappeared, but the pleural mass was unchanged. Video-assisted thoracoscopic surgery was performed. Histopathology revealed dense lymphoplasmacytic infiltration and storiform fibrosis with numerous IgG4-bearing plasma cells. The serum IgG4 level was also elevated. Further examination ruled out the involvement of any other organ. The patient was discharged without further treatment and there is no evidence of recurrence to date.

• 대한한의학방제학회지
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• 제13권1호
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• pp.145-159
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• 2005

Objective : Juglandis Semen has a function that to invigorate the lung and kidney. And It is commonly used as a supporting agent in the treatment of coughing and bronchitis. This study was performed to investigate the effect of oral administration of Juglandis Semen Extract (JSE) against the experimental asthma induced by ovalbumin. Methods : Asthma was induced to Balb/c mouse by i.p. injection and aerosol immunization with ovalbumin. It was observed the change of the cell number in the BAL fluid. Concentrations of IL-4, IL-5 in splenoc yte were assessed by ELISA, IgG and IgE from serum were calculated by same method. Results : 1. Number of macrophage in BAL fluid was significantly decreased in JSE group compared with control group, but not eosinophil and lymphocyte. 2. Levels of IgG and IgE in serum were significantly decreased in JSE group compared with control group, respectively. 3. Concentration of IL-4 in culture supernatant of splenocyte was significantly decreased in JSE group compared with control group, but there was no significant in IL-5. Conclusion : We found that the effect of JSE extract in asthma was implicated in reduction of IL4released from Th2 cell, and decreases of IgG and IgE from plasma cell. These findings suggest that JSE can produce anti-asthmatic effect, which may play a role in allergen-induced asthma therapy.