• Journal of Korean Society of Forest Science
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• v.82 no.3
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• pp.254-259
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• 1993

For the production of mycoplasma-free jujube trees from mycoplasma-infected trees in vitro micrografting visas carried out using apical meristems of in vitro grown plantlets as scions. The rootstocks were hypocotyl segments of in vitro germinated seedlings of Zizyphus jujuba Mill. which is widely used as rootstocks in the field grafting. Ten percent of scions showed normal growth and grew into plants. The presence of mycoplasma was tested using transmission electron microscopy and fluorescence microscopy. Mycoplasma was not found in the tissues of scion parts and seedlings, whereas it was found in in vitro grown plantlets. This suggests that the production of mycoplasmaa-free jujube trees is possible by the in vitro micrografting technique.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.71-75
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• 2000

To clarify the possibility of plant production under red, green. blue, and red+blue using light emitting diodes (LEDs) and fluorescent lamps (control), the effects of light quality on the growth and morphogenesis of in vitro seedlings in Piatycodon grandiflorum were examined. The plantlets grown under the LEDs resulted in taller plants with greater stem than fluorescent lamps. The shortest shoot length, 3.8 cm, was observed in the control and the longest one, 13.4 cm, in the red light. But the shoot length was 5.6 cm under red LED with supplemental blue(red+blue light). This results indicate that red LED may be suitable, in proper combination with other wavelengths of light. The root length under red light was significantly smaller among the treatments. The plantlets grown under red+blue light had lower shoot dry weight, higher dry matter than other lights-grown plantlets. Among the various growth parameters measered, there was an indication that leaf area was controlled by the LEDs. Leaf area of a plantlets developing under green light was about 2.4 times wider than that of plantlets grown under red LED (10.1 $\textrm{cm}^2$ in area). The dry matter rate per plantlet among the treatments was greater in plantlets grown under the red/blue LEDs in comparison with that grown under other LEDs. Chlorophyll contents in plantlets grown under the red, green, blue and red/blue LEDs were 2%, 7% 20% and 10% lower, respectively, than those in plant grown under fluorescent lamps.

• Horticultural Science & Technology
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• v.31 no.4
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• pp.483-489
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• 2013

The abnormal seedlings, a common physiological anomalies, emerged during embryo rescue severely restricted grape breeding. To enhance the efficiency of the seedless grape breeding by reducing the production of abnormal seedlings in the course of embryo rescue, we investigated the effects of genotype, media type, embryo style, pre-chilling on the deformity rate of the abnormal seedlings during embryo rescue. The abnormal seedlings were firstly classified into seven categories based on their morphology. Our results indicated that the emergence of abnormal seedlings was highly dependent on the female parent genotype. Polyembryony was advantageous to diminish the number of abnormal plantlets and the germination rate of embryo was 100%. We also found that pre-chilling treatment could reduce the number of abnormal plantlets and promote the embryo germination. The abnormal plantlets were reduced significantly by the addition of $ZnSO_4$ $10{\mu}mol{\cdot}L^{-1}$ or mashed-banana $500mg{\cdot}L^{-1}$ to either embryo development or germination media. Transferring the abnormal seedlings onto the suitable fresh media in 4 weeks after embryo germination provided an effective way to transform them into normal seedlings.

• Korean Journal of Pharmacognosy
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• v.24 no.4
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• pp.304-308
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• 1993

A platelet activating factor(PAF) antagonist ginkgolides produced from Ginkgo biloba are well known for their potential usage in septic shock and other PAF related diseases. Even though they are extracted from the leaves and on occasion the root bark, the exact biosynthetic site and pathway have not proved yet. In order to locate the enzymes involved and elucidate the biosynthetic site of the compounds, embryo-derived aseptic intact plantlet and plantlet without root have been cultured on 0.3% active carbon-containing solid Murashige and Skoog's medium. The leaves from the six-week-old normal plantlet contained similar amount of ginkgolide B to that of outdoor plant leaves, while the plantlets without root had less than 30% of the ginkgolide B compared to the in vitro intact plantlets. The results suggest that the ginkgolides may be synthesized in the root and transported to the aerial part.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2005.11a
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• pp.190-190
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2006.06a
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• pp.29.2-30
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• 2006

• Horticultural Science & Technology
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• v.30 no.2
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• pp.201-207
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• 2012

This study was conducted to investigate the effect of supporting material and ionic strength of the MS medium on growth of strawberry plantlets cultured in vitro for the rapid mass production. Explants of $Fragaria$ $ananassa$ 'Houkouwase' in vitro were planted in the agar or Tosilee (commercial plug medium) medium as the supporting material and supplied with 1/4 MS, 1/2 MS or basal (as the control) MS medium in an autotrophic micropropagation. Plant height and root length were significantly greater when they were cultured in 1/2 MS medium as compared to those grown in the agar medium. Also, shoot fresh and dry weights, and leaf area in the 1/2 MS medium were greater than in 1/4 MS or basal MS medium. When plantlets were cultured in Tosilee medium and fed with the basal MS medium, plant height, root length, shoot fresh and dry weights, root fresh and dry weights, and leaf area were promoted and greater than those in plantlets cultured in the agar medium.

• Korean Journal of Plant Resources
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• v.12 no.2
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• pp.139-144
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• 1999

The present study was carried out to determine the effects of lighting directions and light intensities on the growth of in vitro plantlets of strawberry, potato and lily. The growth of plantlets was affected by two different lighting systems, downward and sideward lighting system. There were no difference for the plantlets of strawberry regardless of lighting directions. However, leaf area, fresh weight and dry weight have increased as the light intensity increased. Also, plant height and root length on node culture of potato decreased at high intensity of the sideward lighting, while thickness of stem and root was thicker, fresh weight and dry weight were heavier, and leaf area was increased. Also, bulblet formation on scale culture of lily has been abundant, and fresh weight and dry weight were increased. Thus, the sideward lighting system which loaded three stories for culture vessels with raising light intensity into culture vessels instead of conventional downward lighting system promoted growth and raised the efficiency of production of high quality microplant.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.678-686
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• 1997

This experiment was carried out to develop the mass propagation system for producing plug plantlets using stem cuttings of virus-tree microtubers in potato. Cocopeat, vermiculite, perlite and peatmoss were combined and used as plug nursery media to find out the best combination suitable for the growth of seedlings derived from microtubers. Seedling growth was favored in high temperature (above 2$0^{\circ}C$) and a long-day photoperiod(above 16 hours) condition, while stolons and microtubers formed in outdoor condition. Shoot and root multiplication was not affected by NAA 10mg /1 or IAA 10mg /1 treatment. At the early growth stage of plug plantlets, the number of leaves and roots and the length of root increased significantly when nodes from the upper (near to apex) part of shoots rather than from basal part were taken. But after transplanting, these differences among these characters were not observed. At ninety days after transplanting the plug plantlets in spring time, plant was around 70 to 80cm in height, and the number of stolons and tubers were ten and seven, respectively.

• Korean Journal of Agricultural Science
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• v.40 no.4
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• pp.271-276
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• 2013

This experiment was carried out to determine optimal culture conditions for the production of tubers of Calla (Zantedeschia elliottiana 'Golden Affair' and 'Black Magic') in Korea highland. In vitro produced plantlets and tuberlets of Calla 'Golden Affair' and 'Black Magic' were planted plastic film greenhouse and grown for 100, 120, 140 days, with different night temperature treatments ($0{\sim}10^{\circ}C$ : no heating, 10, $15^{\circ}C$). In both cultivars, tuber size(tuber diameter, tuber height) and tuber weight increased with increasing cultivation period when the night temperature was maintained at $10^{\circ}C$. The largest tuber diameter in vitro produced plantlets was 5.8cm in 'Black Magic' and 3.2cm in 'Golden Affair', and daily tuber growth rate was 1.110g in 'Black Magic' and 0.092g in 'Golden Affair' under the culture conditions. Consequently we think that tuber harvest date was Oct. 30 and night temperature was $10^{\circ}C$ and no heating that was proper method of tuber production. However we had selection of $10^{\circ}C$ treatment for tuber production because it appeared freezing damage occasionally in highland late in October.