• The Korean Journal of Mycology
• /
• v.22 no.3
• /
• pp.276-280
• /
• 1994

To obtain hybrids producing antagonisms and plant growth promoting effects by intergeneric nuclei transfer, the nuclei were isolated from the protoplasts of Trichoderma harzianum T95 and treated with colchicine. The nuclei were tranferred into protoplast of multi-auxotrophic Gliocladium virens G88 which cannot grow in minimal medium. The nuclei tranferred into protoplasts of G. virens G88 were selected on the regeneration minimal medium containing chloroneb as a haploid inducer. Low transfer frequency of 0.08% was observed with three chemical treatment, however no segregants were found in the intergeneric nuclei transfer. The various types of hybrids with different morphology were detected when different concentration of chloroneb were treated. These morphologies were classified as parental, recombinant and petite type.

• Journal of Ocean Engineering and Technology
• /
• v.32 no.5
• /
• pp.310-323
• /
• 2018

In order to protect lives and prevent large-scale injuries in the event of a fire on a ship or an offshore plant, most classification societies are strengthening their fire resistance designs of relevant cargo holds and accommodation compartments to keep flames from being transferred from a fire point to other compartments. Particularly in critical compartments, where flames should not propagate for a certain period of time, such as the A60 class division, both the airtightness and fire-resistant design of a piece passing through a bulkhead are subject to the Safety of Life at Sea Convention (SOLAS) issued by the International Maritime Organization (IMO). In order to verify the suitability of a fire-resistant design for such a penetrating piece, the fire test procedure prescribed by the Maritime Safety Committee (MSC) must be carried out. However, a numerical simulation should first be conducted to minimize the time and cost of the fire resistance test. In this study, transient thermal analyses based on the finite element method were applied to investigate the heat transfer characteristics of a bulkhead penetration piece for the A60 class compartment. In order to determine a rational bulkhead penetration piece design, the transient heat transfer characteristics according to the variation of design parameters such as the diameter, length, and material were reviewed. The verification of the design specification based on a numerical analysis of the transient heat transfer performed in this study will be discussed in the following research paper for the actual fire protection test of the A60 class bulkhead penetration piece.

• Korean Journal of Plant Resources
• /
• v.10 no.2
• /
• pp.200-210
• /
• 1997

The understanding on the plant genome is accelerated with the fast advance of molecular biological techniques. The molecular dissecting of the plant genome has made possible the precise genotyping the plants, which can be utilized for molecular breeding program. As well, the molecular cloning of genes interested can facilitate the process of gene transfer between intra-and inter-generic taxa. Moreover, the manipulation of the agronomically important QTL genes, which can be rarely performed by the conventional genetic methods, is also possible by the utilization of molecular markers. In addition to these genetical applications, molecular markers are useful in the areas of plant taxonomy and management of germplasm by fingerprinting analysis. This paper describes the theoretical aspects marker technologies and practical applications of each marker technique.

• Proceedings of the Botanical Society of Korea Conference
• /
• 1987.07a
• /
• pp.9-18
• /
• 1987

The plant breeding, a discipline of agricultural sciences, has greatly contributed to huan welfare in relieving food crisis by development of higher yielding, stronger resistant and better quality varieties. However, many conventional plant breeders, especially ones working for major crops, are facing exhaustion of useful genetic variability, which greatly limit the potentional of developing better cultivars. Therefore, the convectional plant breeders have been eagerly looking for new renovational methods in creating genetic varibility. It has been expected that biotechnology would provide the technique to create totally new genetic variability through gene transfer, chromosome manipulation and/or cell fusion. It is strongly suggested that very close interdisciplinary approaches between convectionla plant breeders and biotechnoligists is essentional for opening new era in developing better varieties.

• Journal of Plant Biotechnology
• /
• v.36 no.2
• /
• pp.144-148
• /
• 2009

The transfer of Mn-Superoxide Dismutase (SOD2) gene, complex gene (SA) of CuZnSOD and ascorbate peroxidase (APX), and NDP kinase 2 (NDPK2) gene into Korean 4 cultivars (cvs. Millenium White, Glory Blue, Glory Red, and Glory Purple) and 15 purebred lines of petunia was conducted using Agrobaterium-mediated technique. Two (Wongyo A2-16 and A2-36) of 15 purebred lines and one (cv. Glory Red) of 4 cultivars were effective for the transfer of SOD2 gene. The putative transgenic plants survived on the 2nd selection medium were 124. From PCR analysis, 118 (derived from 4 cultivars and 2 purebred lines) of 124 plants were confirmed to contain marker (npt II ) gene, while 58 of 118 plants did not have target genes. There were no plants with both npt II and SA genes. Twenty seven of 28 SOD2 transgenic plants were re-confirmed as transformants by Sothern analysis. SOD2 and NDPK2 genes were expressed in the transgenic petunias as the ratio of 77.8 to 100.0 % and 23.5%, respectively. T1 seeds were obtained from 36 acclimated transgenic plants (SOD2 34 plus NDPK2) in a glasshouse by self-pollination.

• Journal of the Korean Society of Manufacturing Technology Engineers
• /
• v.26 no.2
• /
• pp.243-250
• /
• 2017

A numerical study was conducted to calculate the cooling capacity variation of a power plant ACC (air-cooled condenser) caused by changes in operating conditions. A numerical model was developed using the ${\varepsilon}-NTU$ and finite volume method, containing 100 elements for a single low fin tube. The model was validated through a comparison of cooling capacity between the simulated values and manufacturer's data. Even though simple assumptions and previously presented heat transfer correlations were applied to the model, the prediction error was 1.9%. The simulated variables of the operating conditions were air velocity, air temperature, and mass flux. The analysis on the variation of thermal resistance along the tube showed that the water side thermal resistance was higher than the air side thermal resistance at the downstream end of the tube, indicating that the ACC capacity could be increased by applying technology to enhance in-tube flow condensation heat transfer.

• Journal of Mechanical Science and Technology
• /
• v.19 no.5
• /
• pp.1206-1215
• /
• 2005

The thermal stratification phenomena, frequently occurring in the component of nuclear power plant system such as pressurizer surge line, steam generator inlet nozzle, safety injection system (SIS), and chemical and volume control system (CVCS), can cause through-wall cracks, thermal fatigue, unexpected piping displacement and dislocation, and pipe support damage. The phenomenon is one of the unaccounted load in the design stage. However, the load have been found to be serious as nuclear power plant operation experience accumulates. In particular, the thermal stratification by the turbulent penetration or valve leak in the SIS and SCS pipe line can lead these safety systems to failure by the thermal fatigue. Therefore in this study an 1/10 scaledowned experimental rig had been designed and installed. And a series of experimental works had been executed to measure the temperature distribution (thermal stratification) in these systems by the turbulent penetration, valve leak, and heat transfer through valve. The results provide very valuable informations such as turbulent penetration depth, the possibility of thermal stratification by the heat transfer through valve, etc. Also the results are expected to be useful to understand the thermal stratification in these systems, establish the thermal strati­fication criteria and validate the calculation results by CFD Codes such as Fluent, Phenix, CFX.

• Transactions of the Korean Society of Pressure Vessels and Piping
• /
• v.7 no.4
• /
• pp.38-43
• /
• 2011

For the efficient and safe operation of nuclear power plant, evaluating quantitatively aging phenomenon of major components is necessary. Especially, typical aging parameters such as stresses and cumulative usage factors should be determined accurately to manage the lifetime of the plant facility. The 3-D finite element(FE) model is generated to calculate the aging parameters. Mechanical and thermal transfer functions called Green's functions are developed for the FE model with standard step input. The stress results estimated from transfer functions are verified by comparing with 3-D FE analyses results. Lastly, we suggest an effective fatigue evaluation methodology by using the transfer functions. The usefulness of the proposed fatigue evaluation methodology can be maximized by combining it with an on-line monitoring system.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.19 no.10
• /
• pp.2586-2594
• /
• 1995

Two phase flow phenomena are observed in many industrial facilities and make much importance of optimum design for nuclear power plant and various heat exchangers. This experimental study has been investigated the classification of the flow pattern, the local void distribution and convective heat transfer in swirl and non-swirl two phase flow under the isothermal and nonisothermal conditions. The convective heat transfer coefficients in the single phase water flow were measured and compared with the calculated results from the Sieder-Tate correlation. These coefficients were used for comparisons with the two-phase heat transfer coefficients in the flow orientations. The experimental results indicate, that the void probe signal and probability density function of void distribution can used into classify the flow patterns, no significant difference in voidage distribution was observed between isothermal and non-isothermal condition in non-swirl flow, the values of two phase heat transfer coefficients increase when superficial air velocities increase, and the enhancement of the values is observed to be most pronounced at the highest superficial water velocity in non-swirl flow. Also two phase heat transfer coefficients in swirl flow are increased when the twist ratios are decreased.

• Nuclear Engineering and Technology
• /
• v.52 no.5
• /
• pp.918-924
• /
• 2020

Film boiling is of great importance in nuclear safety as it directly influences the integrity of nuclear fuel in case of accidents involving loss of coolant. Recently, nuclear power plant safety under earthquake conditions has received much attention. However, to the best of our knowledge, there are no existing studies reporting film boiling in an oscillating system. Most previous studies for film boiling were performed on stationary systems. In this study, numerical simulations were performed for saturated film boiling of water on a horizontal surface under low frequencies to investigate the effect of system oscillation on film boiling heat transfer. A coupled level-set and volume-of-fluid method was used to track the interface between the vapor and liquid phases. With a fixed oscillation amplitude, overall, heat transfer decreases with oscillation frequency. However, there is a frequency region in which heat transfer remains nearly constant. This lock-on phenomenon occurs when the oscillation frequency is near the natural bubble release frequency. With a fixed oscillation frequency, heat transfer decreases with oscillation amplitude. With a fixed maximum amplitude of the additional gravity, heat transfer is affected little by the combination of oscillation amplitude and frequency.