• Elastomers and Composites
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• 제41권4호
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• pp.231-237
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• 2006

부틸고무를 결합재로 사용하여 과산화수소 정량 바이오센서를 제작하고, 그것의 전기화학적 특성과 실용화 가능성을 살펴보았다. 전극은 본 실험실에서 연구되었던 실리콘 오일을 결합재로 사용한 동식물조직 바이오센서보다 수백 배 이상 큰 신호를 보여주었으며, $0.0{\sim}-1.00\;V$(vs. Ag/AgCl)의 넓은 퍼텐셜 영역에서 안정적으로 작동될 수 있었다. 또 신호전류는 실험 퍼텔셜 영역에서 전극전위에 따라 좋은 직선성을 보였다. 검출한계는 $3.0{\times}10^{-4}\;M$ 이었으며, Lineweaver-Burk 도시의 직선성은 효소가 전극 표면에 효과적으로 고정되어 있음을 확인시켜 주었다. 높은 감도에 의한 신호전류의 재현성과, 부틸고무의 결합력에 의한 전극물질의 기계적 안정성은 전극의 실용화 가능성을 보여 주었다.

• BMB Reports
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• 제54권10호
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• pp.516-521
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• 2021

Although arginase primarily participates in the last reaction of the urea cycle, we have previously demonstrated that arginase II is an important cytosolic calcium regulator through spermine production in a p32-dependent manner. Here, we demonstrated that rhaponticin (RPT) is a novel medicinal-plant arginase inhibitor and investigated its mechanism of action on Ca²⁺-dependent endothelial nitric oxide synthase (eNOS) activation. RPT was uncompetitively inhibited for both arginases I and II prepared from mouse liver and kidney. It also inhibited arginase activity in both aorta and human umbilical vein endothelial cells (HUVECs). Using both microscope and FACS analyses, RPT treatments induced increases in cytosolic Ca²⁺ levels using Fluo-4 AM as a calcium indicator. Increased cytosolic Ca²⁺ elicited the phosphorylations of both CaMKII and eNOS Ser1177 in a time-dependent manner. RPT incubations also increased intracellular L-arginine (L-Arg) levels and activated the CaMKII/AMPK/Akt/eNOS signaling cascade in HUVECs. Treatment of L-Arg and ABH, arginase inhibitor, increased intracellular Ca²⁺ concentrations and activated CaMKII-dependent eNOS activation in ECs of WT mice, but, the effects were not observed in ECs of inositol triphosphate receptor type 1 knockout (IP3R1^-/-) mice. In the aortic endothelium of WT mice, RPT also augmented nitric oxide (NO) production and attenuated reactive oxygen species (ROS) generation. In a vascular tension assay using RPT-treated aortic tissue, cumulative vasorelaxant responses to acetylcholine (Ach) were enhanced, and phenylephrine (PE)-dependent vasoconstrictive responses were retarded, although sodium nitroprusside and KCl responses were not different. In this study, we present a novel mechanism for RPT, as an arginase inhibitor, to increase cytosolic Ca²⁺ concentration in a L-Arg-dependent manner and enhance endothelial function through eNOS activation.

• 한국육종학회지
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• 제41권4호
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• pp.510-514
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• 2009

난 시장이 확대되고 있지만 일반대중이 선호하고 있는 동양계 심비디움 난은 대부분 수입 또는 산채에 의존하여 고가로 판매되고 있고, 서양계 심비디움은 외국 품종에 대한 로열티 지불 등의 문제가 대두되고 있다. 본 연구에서는 관상가치가 있고 대량증식이 가능하여 외국산 품종을 대체할 수 있는 소형 심비디움 난을 육성하고자 하였다. 동양계 및 서양계 심 비디움의 교배종인 "동양"의 바이러스 무병주를 생장점배양에 의해 기내에서 대량증식을 하던 중, 잎무늬 돌연변이 식물체를 선발하여 고정계통을 "대국"이라 명명하였다. "대국"은 잎의 가장자리에 백색이나 노란색 줄무늬가 있는 복륜 무늬종으로 광택이 있다. "대국"의 잎 길이는 약 40 cm, 엽폭은 1.6 cm, 엽수는 4~5장으로 동양에 비교하여 잎 및 초세가 작고 단아하다. 꽃에도 복륜무늬가 있으며 개화시기는 3~5월과 9~10월 연 2회이다. 신품종 "대국"은 잎무늬 줄무늬와 초세가 단정하여 분화용 엽예품으로 관상가치가 뛰어나며, 기존 동양계 심비디움 품종에 비교하여 조직배양 및 순화가 잘되고 생육이 왕성하여 값싸게 공급 가능한 품종이다.(품종특허 등록번호: 특허 제 0478734).

• 문화기술의 융합
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• 제4권4호
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• pp.167-171
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• 2018

알스트로메리아는 경제적으로 중요한 절화 작물 중 하나이다. 그러나 낮은 증식률과 번식기간이 길고 바이러스 질병 감염이 높기 때문에 대량증식이 어렵다고 알려져 있다. 따라서 기내 조직배양을 이용한 대량증식 시스템 쳬계 확립이 필요하다. 본 연구에서는 BA와 NAA 그리고 여러 가지 배지 고형제를 이용하여 알스트로메리아 최적의 생장 효율을 찾기 위하여 실험을 진행하였다. 먼저 BA 1.0 mg/L 와 NAA 0.1 mg/L를 첨가한 배지에서 무처리구에 비하여 신초 및 뿌리의 개수가 약 1.5배 증가하였고 비대한 신초와 뿌리를 나타내었다. 여러 가지 배지 고형제들 중 gelrite 0.25%를 첨가한 배지에서 타 처리구에 비해 최대 50% 향상된 길이를 나타내었고, 근경 개수 및 생체중 증가량에 있어서도 가장 높은 효율을 나타내었다. 본 연구결과를 토대로 알스트로메리아 신품종 개발 및 대량증식 시스템 체계 확립에 도움을 줄 것으로 기대된다.

• 한국축산식품학회지
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• 제38권6호
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• pp.1155-1159
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• 2018

Foot-and-mouth disease (FMD) is an infectious disease affecting pigs. The control of FMD in swine husbandry is very important because its outbreak results in a vast economic loss. FMD vaccination has effectively controlled FMD; however, it results in economic loss associated with the incidence of lesions in the pork meat at the injection site. The objective of this study was to investigate the effects of transdermal needle-free injection (NFI) of the FMD vaccine on the incidence of lesions at the injection site. Pigs (n=493) in the control group were vaccinated with the FMD vaccine using a commercial syringe needle, while 492 pigs in the transdermal NFI group received the FMD vaccine using a needle-free gas-powered jet injector. After the slaughter of the pigs, the incidence of lesions at the injection site of all pigs was checked by plant workers. The result of this study showed that the incidence of lesions in the pork ham from pigs vaccinated with NFI was 14.82% lower than that in control pigs (p<0.01). In addition, lesions generated in the NFI group were found just in the subcutaneous tissue. Therefore, the incidence of lesions at the injection site in pork from pigs vaccinated with the FMD vaccine can be effectively reduced by using transdermal NFI rather than a conventional syringe needle.

• 한국산업융합학회 논문집
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• 제22권1호
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• pp.61-69
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• 2019

All the parts of lotus, including the seed, rhizome, leaf, stalk, petal, anther, pericarp, and fruit receptacle, have been used in traditional medicine system as a health beneficial supplement. However the most usually used from lotus plant is only the root. Therefore in this study, it will be discussed more the utilization of other parts of the lotus, namely the flower of lotus. The petals and stamens of lotus actually are also rich in bioactive components such as flavonoids and alkaloids, are used in the treatment of tissue inflammation, cancer, skin disease, and also for us as antidotes. One of the biotechnological process that can be used to improve the nutritional content, sensory, and also antioxidant activities is fermentation process. The final product desired from the fermentation process in this study is vinegar. The microbial strain powder used is Uinkin fermented powder with three variations of fermentation. The variations given in this study were initial sugar 32%, 24%, and 14% with the same fermentation temperature, $35^{\circ}C$ for 3 months. The results obtained showed that the pH value and sugar content of products during the fermentation process were decreasing during the fermentation process, with total polyphenol content of $283.7{\pm}97.6mg/100g\;QAE$, and total flavonoid content of $3.3{\pm}0.0mg/100g\;QAE$. For the DPPH radical scavenging ability of the fermentation product also increased in a concentration dependent manner, with ORAC activity of the product showed a high activity of $20.7{\pm}0.41{\mu}M$ TE. Therefore, fermentation process can be the one of method for improving the product. The efficiency of lotus flower vinegar fermentation can be reached with an initial sugar condition of 25% (sample B).

• Horticulture, Environment, and Biotechnology : HEB
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• 제59권6호
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• pp.909-917
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• 2018

Quercetin and its glycoside derivatives were identified and quantified using high-performance liquid chromatograph (HPLC) and liquid chromatograph/mass spectrometer/mass spectrometer (LC/MS/MS) in the leaves, flowers, and fruits of 22 Malus genotypes. In all genotypes, small amounts of quercetin aglycone were present, with water-soluble glycoside forms were the most abundant in different Malus plant tissues, including quercetin-3-galactoside, quercetin-3-rutinoside, quercetin-3-glucoside, quercetin-3-xyloside, quercetin-3-arabinoside, and quercetin-3-rhamnoside. Among these six quercetin glycosides, quercetin-3-galactoside was the common form in Malus plants, except in the leaves and flowers of M. ceracifolia and M. magdeburgensis, and in the fruits of M. micromalus 'Haihong Fruit', where there was a higher concentration of quercetin3-glucoside. Among the different tissues tested, leaves contained the highest concentration of quercetin and its glycosides, while fruits contained the lowest concentrations of these compounds. Among the genotypes we analyzed, no specific genotype consistently contained the highest concentration of quercetin and its glycoside derivatives. M. domestica 'Honeycrisp' had the highest total compound concentration (approximately $1600mg\;kg^{-1}$), whereas M. hupehensis contained the lowest in its fruits. In contrast, the concentration of total quercetin and its glycosides was more than $5000mg\;kg^{-1}$ in the leaves of eight genotypes and greater than $2500mg\;kg^{-1}$ in the flowers of seven species. In general, the concentration of quercetin and its glycoside derivatives depended on the species and tissue type. These results may provide useful information for the evaluation and selection of edible Malus fruits and the materials for quercetin glycoside extraction.

• Journal of Microbiology and Biotechnology
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• 제31권9호
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• pp.1272-1280
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• 2021

With changes in human dietary patterns, the proportion of high-fat and high-cholesterol foods in the daily diet has increased. As a result, the incidence rate of cholelithiasis is increasing rapidly. Many studies have reported on the crucial role that the intestinal microflora plays in the progression of gallstones. Although the whole herb of Lysimachia christinae, a traditional Chinese medicine, has long been extensively used as a remedy for cholelithiasis in China, its effects on the intestinal microflora remain unknown. Hence, in this study, we investigated the ability of the aqueous extract of L. christinae (LAE) to prevent cholesterol gallstones (CGSs) in model animals by affecting the intestinal microflora. The effects of LAE on body weight, serum lipid profile, visceral organ indexes, and histomorphology were studied in male C57BL/6J mice, which were induced by a lithogenic diet. After the 8-week study, CGSs formation was greatly reduced after LAE treatment. LAE also reduced body weight gain and hyperlipidemia and restored the histomorphological changes. Moreover, the intestinal microflora exhibited significant variation. In the model group fed the lithogenic diet, the abundances of the genera unclassified Porphyromonadaceae, Lactobacillus and Alloprevotella decreased, but in contrast, Akkermansia dramatically increased compared with the control check group, which was fed a normal diet; the administration of LAE reversed these changes. These results imply that L. christinae can be considered an efficient therapy for eliminating CGSs induced by a high-fat and high-cholesterol diet, which may be achieved by influencing the intestinal microflora.

• Journal of Ginseng Research
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• 제45권1호
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• pp.58-65
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• 2021

Background: Panax ginseng, as one of the most widely used herbal medicines worldwide, has been studied comprehensively in terms of the chemical components and pharmacology. The proteins from ginseng are also of great importance for both nutrition value and the mechanism of secondary metabolites. However, the proteomic studies are less reported in the absence of the genome information. With the completion of ginseng genome sequencing, the proteome profiling has become available for the functional study of ginseng protein components. Methods: We optimized the protein extraction process systematically by using SDS-PAGE and one-dimensional liquid chromatography mass spectrometry. The extracted proteins were then analyzed by two-dimensional chromatography separation and cutting-edge mass spectrometry technique. Results: A total of 2,732 and 3,608 proteins were identified from ginseng root and cauline leaf, respectively, which was the largest data set reported so far. Only around 50% protein overlapped between the cauline leaf and root tissue parts because of the function assignment for plant growing. Further gene ontology and KEGG pathway revealed the distinguish difference between ginseng root and leaf, which accounts for the photosynthesis and metabolic process. With in-deep analysis of functional proteins related to ginsenoside synthesis, we interestingly found the cytochrome P450 and UDP-glycosyltransferase expression extensively in cauline leaf but not in the root, indicating that the post glucoside synthesis of ginsenosides might be carried out when growing and then transported to the root at withering. Conclusion: The systematically proteome analysis of Panax ginseng will provide us comprehensive understanding of ginsenoside synthesis and guidance for artificial cultivation.

• 화훼연구
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• 제26권4호
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• pp.166-178
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• 2018

In this study, the principal objective was to investigate the effect of light quality and vessel ventilation on the growth and development, physiology, activities of antioxidant enzymes, and contents of mineral nutrients of carnation (Dianthus caryophyllus L.) 'Marble Beauty'. Single node cuttings stuck into the plant growth regulator (PGR)-free MS medium in containers covered with caps with or without a ventilation filter were cultured first four weeks under white and then additional four weeks under either white (control), blue, red, or red + blue light emitting diodes (LEDs) for 56 days. Interestingly, a ventilated culture condition not only reduced the percentage of the hyperhydricity, but also increased the total chlorophyll content (Chl a + Chl b) of the plantlets as compared to the non-ventilated condition. In addition, blue LEDs produced plantlets with the greatest number of shoots and red LEDs produced plantlets with the greatest shoot length. The quality of plantlets was improved under a ventilation condition. Besides, under a ventilated condition, red + blue LEDs raised APX activity, and blue LEDs not only raised the activity of the CAT, but also increased tissue contents of such elements as K, Ca, Mg, Zn, Mn and Fe. The red LEDs increased contents of B and Si under a ventilated condition, and Na accumulation under a non-ventilated condition. Thus, including blue or red LEDs as the light source in a ventilated culture condition will produce plantlets of carnation 'Marble Beauty' in vitro with improved quality.