• KSBB Journal
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• v.30 no.3
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• pp.103-108
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• 2015

Production of foreign proteins by transgenic plant cell cultures has several advantages such as post-translational modification, low risk of product contamination and low-cost production and purification. However, target proteins are degraded by extracellular proteases existing in the media. A solution to this problem is the use of perfusion culture and ion exchange chromatography for the application of integrated bioprocess using in situ recovery. With this method, production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was investigated in this study. First, optimization of cell concentration during the induction phase for the production of hGM-CSF was examined. As cell concentration increased, the level of hGM-CSF was decreased due to the presence of extracellular proteases. Induction using sugarfree media produced 33% more hGM-CSF. The effects of pH on the binding of hGM-CSF to cationic and anionic exchange resins were also investigated. In terms of stability, optimal pH was found to be 5~7. In the case of using buffer exchange when CM-Sepharose was used as a cationic exchange resin, optimal pH for binding was 4.8 and adsorption yield was 77%. When DEAE-Sepharose was used as an anionic exchange resin, it was 5.5 (74%). Without buffer exchange, optimal pH was 4.6 (84%). From these results, an integrated bioprocess using in situ recovery with simultaneous production and separation of foreign protein in transgenic plant cell suspension cultures was found to be feasible.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11c
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• pp.639-646
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• 2000

Hot air heater with light oil combustion is the most common heater for greenhouse heating in the winter season in Korea. However, since the heat efficiency of the heater is about 80%, considerable unused heat in the form of exhaust gas heat discharges to atmosphere. In order to capture this exhaust gas heat a heat recovery system for plant bed heating in the greenhouse was built and tested in the hot air heating system of greenhouse. The system consists of a heat exchanger made of copper pipes, ${\phi}\;12.7{\times}0.7t$ located inside the rectangular column of $330{\times}330{\times}900mm$, a water circulation pump, circulation plastic pipe and a water tame The total heat exchanger area is $1.5m^2$, calculated considering the heat exchange amount between flue gas and water circulated in the copper pipes. The system was attached to the exhaust gas path. The heat recovery system was designed as to even recapture the latent heat of flue gas when exposing to low temperature water in the heat exchanger. According to performance test it can recover 45,200 to 51,000kJ/hr depending on the water circulation rates of 330 to $690{\ell}$/hr from the waste heat discharged. The exhaust gas temperature left from the heat exchanger dropped to $100^{circ}C$ from $270^{circ}C$ by the heat exchange between the water and the flue gas, while water gained the difference and temperature increased to $38^{circ}C$ from $21^{circ}C$ at the water flow rate of $690{\ell}$/hr. And, the condensed water amount varies from 16 to $43m{\ell}$ at the same water circulation rates. This condensing heat recovery system can reduce boiler fuel consumption amount in a day by 34% according to the feasibility study of the actual mimitomato greenhouse. No combustion load was observed in the hot air heater.

• Proceedings of the Membrane Society of Korea Conference
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• 2002.07a
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• pp.1-12
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• 2002

Desalination by reverse osmosis (RO), which first entered commercial use in the 1970s, was initially mainly used for treating brackish water. Technological progress led to the development of an RO membrane enabling single-pass seawater desalination. Toyobo succeeded in developing a single-pass seawater desalination RO module composed of hollow fiber type membranes made of cellulose triacetate in 1978, and then in 1979 began production of the first commercially available double-element module. This double-element module has many advantages suitable for seawater desalination. It has high chlorine tolerance and high salt rejection, derived from the properties of the membrane material, and it is highly resistant to fouling and scaling matters due to the unique flow pattern and fiber bundle configuration. These advantages help to explain why the Toyobo double-element module has been used so successfully at the many seawater desalination plants around the world. Since the 1980s, large plants capable of desalinating several tens of thousands of cubic meters a day have sprung up around the Mediterranean and In the Middle East. The Jeddah RO Phase I Plant, which has a capacity of 56, 800m$^3$/day, went into operation in 1989. In 1994, the same sized Phase II Plant came on stream, giving the plant a huge total capacity of 113, 600m$^3$/day. The plant constructor Mitsubishi Heavy Industries, Ltd. (MHI), and the RO membrane manufacturer Toyobo Co., Ltd. In 1998, the world's largest RO seawater desalination plant in operation, which has a capacity of 128, 000m$^3$/day and is run by Saudi Arabia's Saline Water Conversion Corporation (SWCC), went into operation at Yanbu. RO seawater desalination technology has thus already reached the stage of full-scale commercial use. In order to encourage its wider use, however, RO desalination needs to be made more economical by lowering construction and water treatment costs. Toyobo has therefore developed a new economical RO desalination system by a recovery ratio of 60% using a high-pressure module with a high product flow rate. In 2000, Toyobo high recovery membrane module was selected for the largest seawater desalination plant in Japan, which has a capacity of 50, 000m$^3$/day.

• Journal of Korean Society of Water and Wastewater
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• v.12 no.3
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• pp.30-40
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• 1998

Increasing water consumption produced sludge problems of the water treatment plants. The objective of this study is to investigate aluminium coagulants recovery n acidic and alkaline conditions. Water treatment plant sludge produced in Pusan Metropolitan City were tested for the aluminium extraction process. Experiment samples were obtained in summer from water treatment plants of Deoksan and Myongjang. Aluminium coagulants used in these plants during the test period were polyaluminium chloride(PAC), polyaluminium sulfate organic(PSO), polyaluminium sulfate silicate(PASS). Aluminium contents of water treatment sludge were in the range of 7.2~10.9% of the total solids. The recovery percentages for aluminium and iron by acidic extraction method was evaluated to 88% and 42% respectively. Extracted mass variation for other materials such as iron, manganese, total organic carbon was observed during the extraction operation. Alkaline extraction produced more than two times amount of total organic carbon than that in the acidic extraction process.

• Journal of Advanced Marine Engineering and Technology
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• v.23 no.5
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• pp.670-678
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• 1999

Heat recovery steam generator(HRSG) is a principal component of the combined cycle power plant (CCPP) which utilizes the waste energy of the gas turbine exhaust gas. A design of the HRSG is a keypoint to achieve high cycle efficiency with competitive cost. This paper presents a brief review on the design of a HRSG which covers the basic design parameters and their effects on the performance and the investment cost. Finally the concept of the optimum design point is presented according to the selection of a pinch point temperature difference and a steam pressure as an illustrated case.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11b
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• pp.378-387
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• 2000

A rice mill plant with a capacity of 2.5 ton/hr was constructed with automated facilities at Chonnam National University. A simulation model was developed with SLAM SYSTEM for evaluating and improving the rice mill plant. The developed model was validated in the views of hulling efficiency, milling efficiency, milled rice recovery, other materials produced, and bottlenecks in the processes. The results of hulling efficiency, milling efficiency, milled rice recovery in the simulation were, respectively, 81.1%, 89.5%, and 73.1%, while those of the actual mill plant were 81.5%, 90.2%, and 73.5%. The simulation results including the productivity of other materials(chaff, bran, broken rice, stone, etc) produced in the processes were almost similar with those of the actual process. In the simulation the bottlenecks were found out in the processes of separating brown rice and of sorting colored rice. These phenomenon also appeared in the actual process. It needed to increase the hourly capacity of the brown rice separator and the rice color sorter. As the developed model could well express the automated rice mill plant, it could be used for designing and improving rice mill plants. In addition, an alternative model needed to be developed for the system control more accurately and for increasing the rice quality.

• KSBB Journal
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• v.31 no.4
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• pp.284-290
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• 2016

In this research, recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) was produced by transgenic rice cells. RAmy3D promoter was used for overcome the limitation of low expression level in transgenic plant cells, and the secretion of target protein was accomplished by signal peptide. However, the RAmy3D promoter system which can be induced only by sugar starvation causes the decrease of cell viability. As a result, cell death promotes the release of protease which degrades the target proteins. The protein stability and productivity can be significantly influenced by proteolysis activity. Therefore, development of new strategies are necessary for the in situ recovery of target proteins from cell culture media. In this study, in situ recovery was performed by various strategies. Direct addition of Protein A resin with nylon bag leads to cell death by increased shear stress and decrease in production of hCTLA4Ig by protease. Medium exchange through modified flask could recover hCTLA4Ig with high cell viability and low protease activity, on the other hand, the productivity was lower than that of control. When in situ recovery was conducted at day 7 after induction in air-lift bioreactor, 1.94-fold of hCTLA4Ig could be recovered compared to control culture without in situ recovery. Consequently, in situ recovery of hCTLA4Ig from transgenic rice cell culture could enhance productivity significantly and prevent degradation of target proteins effectively.

• Journal of the Korea Organic Resources Recycling Association
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• v.23 no.2
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• pp.21-27
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• 2015

An introduction of landfill tax has been recently in issue in order to raise the financial resource for establishing waste resource-circulation society. The objectives of this study are to evaluate the plan of increasing energy recovery from waste at Incheon Metropolitan City, and to propose several points to be considered in terms of introducing the landfill tax. There are a lot of problems that impede energy recovery from waste at Incheon Metropolitan City, such as high-calorific waste from decrease of food waste to the municipal incineration plant, metals and batteries in the standard plastic garbage bag, etc. Alternative policies to solve these major problems have been drawn.

• Journal of Biosystems Engineering
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• v.20 no.1
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• pp.47-57
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• 1995

Rice Processing Complex(RPC) have being constructed with a rice mill plant and a facility of drying and storage to overcome problems caused by UR and to produce good quality of rice. An optimal design of a rice mill plant was required to successfully construct and operate it. The development of a simulation model was essential to the design of a rice mill plant. So, all the objectives of this study were to develop a simulation model for the design of a rice mill plant and to develop and evaluate the rice mill system. In this study the simulation model was developed to design a rice mill plant using SLAMSYSTEM, one of simulation languages. The results of this study were as followings. 1. A simulation model was developed with SLAMSYSTEM to represent the processes of a rice mill plant. The simulation model was used to design a rice mill pilot plant with the capacity of 0.5 ton per hour. The rice mill pilot plant was analyzed by the model with alternatives. 2. In the simulation the rice mill system was much influenced by the separating efficiency of a brown rice separator. Especially, the bottleneck of grain flow occurred at the buffer tank for brown rica. separator under 50% separating efficiency of brown rice separator. Hence, as the alternative simulation was conducted under 60% , 70% separating efficiency of brown rice separator, the bottleneck of the system could be minimized at the 60% separating efficiency of brown rice separator. 3. In the alternative simulation the bottleneck of the system was minimized under the hulling capacity of 1 t/h and 60% separating efficiency of brown rice separator with the capacity of 1 t/h. Under such a condition the max. weight of waiting entities at buffer tanks was less 250kg. So, the capacities of the buffer tanks were determined in the basis of simulation results. 4. The milled rice recovery and head rice recovery of the milling system were 74% and 92% in the simulation, respectively. These results of simulation almost corresponded to those of actual rice mill plants. The developed simulation model could be well applied to design a rice mill plant.

• 한국신재생에너지학회:학술대회논문집
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• 2010.11a
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• pp.129.2-129.2
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• 2010

As a part of the government renewable energy policy, KOWEPO is constructing 300MW IGCC plant in Taean. IGCC plant consists of gasification block, air separation unit and power block, which performance test is separately conducted. Overall performance test for IGCC plant is peformed to comply with ASME PTC 46. Major factors affected on the overall efficiency for IGCC plant are external conditions, each block performance(gasification, ASU, power block), water/steam integration and air integration. Performance parameters of IGCC plant are cold gas efficiency, oxygen consumption, sensible heat recovery of syngas cooler for gasification block and purity of oxygen, flow amount of oxygen and nitrogen, power consumption for air separation unit and steam/water integration among the each block. The gas turbine capacity applied to the IGCC plant is 20 percent higher than NGCC gas turbine due to the low caloric heating value of syngas, therefor it is possible to utilize air integration between gas turbine and air separation unit to improve overall efficiency of the IGCC plant and there is a little impact on the ambient condition. It is very important to optimize the air integration design with consideration to the optimized integration ratio and the reliable operation. Optimized steam/water integration between power block and gasification block can improve overall efficiency of IGCC plant where the optimized heat recovery from gasification block should be considered. Finally, It is possibile to achieve the target efficiency above 42 percent(HHV, Net) for 300MW Taean IGCC plant by optimized design and integration.