• Journal of Korean Society of Forest Science
• /
• v.86 no.3
• /
• pp.391-398
• /
• 1997

Present study describes a method on the application of efficient tissue culture systems for the micro-propagation of juvenile and mature sawtooth oak(Quercus acutissima). Nodal segments with axillary buds were used as initial explant sources. WPM(Woody Plant Medium) was the best in growth and proliferation of shoot among the media tested. Although the single effect of zeatin revealed on two dorminant shoot elongation with normal growth until the elevation of levels up to 3.0mg/l, BAP($N^6$-benzyl amino purine) usually showed better response than zeatin on shoot multiplication and/or elongation. In addition, the incorporation of BAP and zeatin onto the culture media represents more effectiveness in shoot proliferation and its growth. Optimum concentrations of BAP and zeatin were 0.5 and 0.05~1.0mg/l, respectively. Ninety percent of the proliferated shoots was rooted on half-strength GD (Gresshoff and Doy, 1972) medium containing 0.5mg/l IBA(indole butyric acid) in 4 weeks after culture. More than 70% of the rooted plantlets survived after 5 months of transplanting into artificial soil mix containing equal amount of peatmoss and perlite. Among 27 plus tree clones which were grafted twice onto the juvenile rootstocks, only 4 clones revealed the possibility for shoot multiplication through tissue culture system. The capacity for the micropropagation using mature explant sources was highly depended on clonal differences compared with those of octet age. More than 90% of rooting ratio was obtained from the best responding clone. Among the 7 rooting media tested, GD medium was the best far rooting. The most effective rooting was obtained on half-strength GD medium containing 0.2 to 2.0mg/l IBA. More than 60% of rooted plantlets survived after 5 months of transplanting into the artificial soil mix.

• Journal of Korean Society of Forest Science
• /
• v.83 no.2
• /
• pp.205-210
• /
• 1994

This study was conducted to develop a method of mass production by cutting of superior genotypes of Larix leptolepis and to examine the effects of ortet age, clone, crown position, and an applied plant growth substance upon the rooting of cuttings. Four different ortet ages, 2, 8, 16 and 30 years and three levels of root-promoting substances, indole butyric acid(IBA), at 1000, 2000, and 5000ppm were employed. Greenwood cuttings were taken from mother trees in late July and rooted for three months in rooting medium containing peatmoss : vermiculite : perlite(1 : 1 : 1 by volume). with temperature controlled at $20-25^{\circ}C$, humidity by intermittent misting and light by partial shading in the greenhouse. Cuttings treated with 1000ppm IBA showed highest rooting, with 80%, 71%, 52%, 25% for 2-, 8-, 16-. 30-year-old ortet, respectively. Untreated cuttings showed rooting of 52%, 48%, 36%, 20% for the ortet age of 2, 8, 16, 30 years, respectively. The average number of adventitious roots decreased with increasing ortet age from 2 to 30 years, whereas IBA treatment increased the number of roots in all ages. A variation in rooting among 20 clones tested was observed. The 4 clones, Chungnam 6, and 7 and Chonbuk 1, and 9 showed good rooting of about 67%, while Kangwon 49 showed boor rooting of 40% at 1000ppm IBA treatment. When crown position was compared, cuttings taken from middle crown showed highest rooting of 60%, while cuttings from upper crown showed lowest rooting of 53%.

• Journal of Bio-Environment Control
• /
• v.29 no.3
• /
• pp.219-230
• /
• 2020

This study was conducted to determine the optimum medium and humidification period for the strawberry (Fragaria × ananassa Duch. cv. Maehyang) cutting seedling production. The cuttings were planted in coir (CO), rockwool (RW), phenolic foam (PF), and organic foam medium (OFM) with fogging treatment in 0, 3, 6, 9, or 12 days on February, 2019. And, as the field verification examination research, cuttings were planted in CO, RW, PF, and OFM, with misting treatment in 0, 6, 9, 12, or 15 days on June, 2019. In fogging treatments, rooting and survival rates of seedlings tended to increase with longer fogging periods, and rooting and survival rates were showed significantly higher in CO medium with more than 9 days of fogging periods. In misting treatments, rooting rate was significantly higher in CO and RW medium with more than 9 days of misting periods, and survival rate was significantly higher with more than 12 days of misting periods. There was no significantly difference by growth medium and humidification period in shoot and root growth. Therefore, when considering the rooting and survival rate the CO medium with 9 days of humidification period could be beneficial for the production of the 'Maehyang' strawberry cutting seedlings.

• Journal of Korean Society of Forest Science
• /
• v.80 no.1
• /
• pp.1-8
• /
• 1991

Developmental micropropagation method and somatic embryogenesis for hybrid poplars, Populns ehrarnericana Eco28, P. nigra ${\times}$ P. moximowiczii 62-9, were established using nodule culture system. Calli of Eco28 and 62-9 clone were initiated from leaf explant on the medium with 0.5mg/l and 2.0mg/l 2, 4-D, respectively. Cell suspension culture was established from callus derived from leaf explant culture. When suspended on MS medium with optimal combination of BA and NAA fine nodules were obtained after 2 weeks of culture. For shoot regeneration, nodules were transferred into liquid and agar solidified medium. Numerous shoots were regenerated from nodules of 62-9 on liquid media. Organogenesis was effectively achieved on agar solidified regeneration media containing different concentrations of BA and adenine sulfate. Average numbers of 27 and 24 shoots per nodule were induced from 62-1 and Eco28 clones after 8 weeks of culture, respectively. In addition, somatic embryogenesis also occurred in the same regeneration medium. This procedure can be applied to vegetative propagation, utilization of somaclonal variation, production of secondary metabolite and materials of biotechnology research.

• Journal of the Korean Association of Geographic Information Studies
• /
• v.18 no.3
• /
• pp.89-101
• /
• 2015

Outbreaks of highly pathogenic avian influenza(HPAI) subtype H5N8 have occurred in Korea, January 2014 and it continued more than a year until 2015. And more than 5 million heads of poultry hads been damaged in 196 farms until May 2014. So, we studied the spatial, temporal and spatio-temporal patterns of the HPAI epidemics for understanding the propagation and diffusion characteristics of the 2014 HPAI. The results are expressed using GIS. Throughout the study period three epidemic waves occurred over the time. And outbreaks made three clusters in space. First spatial cluster is adjacent areas of province of Chungcheongbuk-do, Chungcheongnam-do and Gyeonggi -do. Second is Jeonlabuk-do Gomso Bay area. And the last is Naju and Yeongam in Jeollanam-do. Also, most of spatio-temporal clusters were formed in spatially high clustered areas. Especially, in Gomso Bay area space density and spatio-temporal density were concurrent. It means that the effective prevention activity for HPAI was carried out. But there are some exceptional areas such as Chungcheongbuk-do, Chungcheongnam-do, Gyeonggi-do adjacent area. In these areas the outbreak density was high in space but the spatio-temporal cluster was not formed. It means that the HPAI virus was continuing inflow over a long period.

• Horticultural Science & Technology
• /
• v.33 no.1
• /
• pp.149-154
• /
• 2015

A new Phalaenopsis cultivar 'SM 333' was bred by Sangmiwon Orchid, Korea, which produces young plants through tissue culture techniques. The new cultivar 'SM 333', showing the phenotype of multiflora with pink color and small, multibranching-type characteristics, was derived from crossing between Phalaenopsis 'Odoriko' and 'Be Tris'. An elite individual number '02-03-33' later termed 'SM 333' was selected among about 300 individual progenies, based on an intensive selection process covering vegetative and flowering distinctiveness over more than 2 years. In year 2004-2005, the 1st and 2nd characteristic analyses were carried out through performance and uniformity tests. 'SM 333' shows flower color that is bright clean pink (RHS # RP69D) and flower shape that is formal type with 5.0 and 5.8 cm in flower height and width, respectively. 'SM 333' is regarded as raceme flower type suitable for the small casual flower market. The leaves of 'SM 333' grow horizontally and about 20.8 cm in length and 6.5 cm in width. This cultivar also possesses no genetic variation, and is amenable to fast in vitro propagation and easy growth due to its vigorous growth habit. This 'SM333' was registered (Reg. # 2916) with Korea Seed & Variety Service (KSVS) on 1st December, 2009, and the plant breeder's right is currently controlled by Sangmiwon Orchid Company, Korea.

• FLOWER RESEARCH JOURNAL
• /
• v.17 no.4
• /
• pp.285-290
• /
• 2009

This experiment was conducted to investigate the propagation system by seed to check growth, flowering, and making seed of three-year-old cactus of potted Mammillaria goldii. M. theresae, and M. pseudopeclinata. Plant height and width of them was 2.5~2.8 cm, and 2.8~3.1 cm, and seed formation of Mammillaria goldii and M. theresae had internal body taking130 to 135 days to ripening seeds, however, M. pseudopectinata had externals taking just 26 days to ripening seeds. The mean seeded pod from April to June of M. goldii was 50.0% and the number of seeds in a pod was 25.3. In M. theresae, the mean seeded pod from April to June was 57.3% and the number of seeds in a pod was 35.0. However, the highest rate of seeded pod on May of M. pseudopectinata was 75.0% and the number of seed in a pod was 66.0. The sib crossing was much more ripening seed fertilization than that of self cross ing in the Mammillaria. The self crossing was formed from 12.0% to 20.0% of seeded pod, from 12.0 to 16.0 number of seed in a pod. However, sib crossing obtained 58.0% of seeded pod, 30 seeds in a pod of M. goldii, 65.0% seeded pods and 40 seeds in a pod of M. theresae, and 75.0% seeded pod, 68 seeds in a pod of M. pseudopectinala as the highest of them.

• FLOWER RESEARCH JOURNAL
• /
• v.18 no.3
• /
• pp.179-185
• /
• 2010

This study was carried out to investigate the optimal culture conditions for gametophytes growth and sporophytes regeneration of Pyrrosia linearifolia in order to provide for the masspropagation system foundation of Pyrrosia linearifolia using their life cycle. Among many different media, 2MS medium was most effective in prothallus proliferation. Prothallus growth was promoted as the total concentration of nitrogen sources increased, and the best result was observed on 120 mM nitrogen. The best concentration of sucrose was 3%. The addition of 5~20 mM IAA, NAA, BA and kinetin promoted the propagation of prothallus. But 2iP demonstrated the most inhibitory effect on prothallus proliferation. Gametophytes shaking-cultured with liquid medium showed similar growth with solid medium and normal formation of reproductive organs. Shoot regeneration was most effective on 1/8MS medium, but growth was promoted on 1/2MS medium. For promotion of shoot regeneration and growth, the suitable concentrations of sucrose and $NaH_2PO_4$ were 1% and $50{\sim}100mg{\cdot}mL^{-1}$ in 1/8MS medium, respectively.

• Journal of agriculture & life science
• /
• v.45 no.4
• /
• pp.73-80
• /
• 2011

This study was conducted to elucidate the best cutting condition in propagation of Juniperus chinensis var. sargentii in respect of survival rate, rooting rate, and early growth after cutting via process of cutting time, cutting media, cutting type and growth regulators. As for cutting time, rooting rate was 68.3% on April, which was higher than 39.5% on May and 32.5% on June. The best cutting media was stream sand of rooting rate 72.7%. Those of mixed soil and decomposition were 40.1% and 27.5% each. The effect of growth regulators was different according to the kind of cutting media. There was no effect on stream sand media which showed the highest rooting rate, while it was very effective on the media of decomposition of granite, which rooting rate showed the lowest. Hardwood as cutting materials showed somewhat higher survival and rooting rate compared to semi-hardwood. The cutting with hardwood condition to stream sand on April, it is regarded to be the most effective condition for Juniperus chinensis var. sargentii to increase survival and rooting rate. Therefore in this experiment it is considered that the best season cutting is April, and the most useful media and plant materials stream sand, and hardwood respectively.

• Korean journal of applied entomology
• /
• v.60 no.4
• /
• pp.423-429
• /
• 2021

The ovipositional characteristics of the endangered species Prosopocoilus astacoides blanchardi was investigated for indoor mass-rearing. This insect required 3 weeks or more for mating after emergence when introduced at the 1:1 ratio of male to female. Among temperatures studied at 16, 20, 24, 28, and 32℃, the pre-ovipositional period of P. astacoides blanchardi was longest at 16℃ with an average of 109 days after emergence, while it was shortest at 32℃ with an average of 59.4 days. The number of eggs laid at 24℃ was highest with an average of 40.6 per female. The average lifespan of adults at 16℃ was 208.8 days for males and 263.9 days for females, while at 32℃ 96.1 days for males and 130.3 days for females. The number of laid eggs was dependent on insect rearing chamber size. The oviposition-trees did not significantly (P > 0.05) affect the number of laid eggs and ovipositional period. The results of this study could be used as rearing data for the restoration and mass propagation of the endangered species P. astacoides blanchardi.