• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1999.12a
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• pp.227-233
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• 1999

• Journal of the Korean Society of Tobacco Science
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• v.33 no.1
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• pp.8-15
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• 2011

Genus Nicotiana has 76 species including N. tabacum. These plants are used not only as a material for cigarette manufacturing but also as ornamental plant, medicinal plant, poisonous substance plant, and bug repellent plant. N. tabacum is used as a main material for cigarette manufacturing with N. rustica. N. sylvestris and N. alata is used as ornamental plants because of their beautiful flowers and N. rustica is used for bug repellent or pesticide because of its high concentration of nicotine. N. glauca, a tree tobacco, is used for bio-fuel production. N. tabacum is used as a popular model plant system for degeneration, regeneration, and transformation. N. benthamiana is also used as a model system for foreign gene expression by agroinfiltration. The transformation ability of tobacco plant is a good target for molecular farming. Hepatitis B virus envelop protein, E. coli heat-labile enterotoxin, diabetes autoantigen, and cholera toxin B subunit were produced using tobacco plants. Secondary metabolites of tobacco include nicotine, anabasine, nornicotine, anatabine, cembranoid, solanesol, linoleic acid, rutin, lignin and sistosterol, and they are used for various medicine productions which cannot be produced by organic synthesis for their complicated structures. In conclusion, we have to understand the applicability of tobacco plant in detail and study to enlarge the usage of the plants.

• The Plant Pathology Journal
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• v.23 no.3
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• pp.223-225
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• 2007

Many plant growth-promoting rhizobacteria (PGPRs) have been known for beneficial effects on plants including biological control of soilborne pathogens, induced systemic resistance to plant pathogens, phytohormone production, and improvement of nutrient and water uptake of plants. We developed a simple and rapid method for screening potential PGPR, especially phytohormone producing rhizobacteria, or for analyzing their functions in plant growth using cucumber seedling cuttings. Surface-sterilized cucumber seeds were grown in a plastic pot containing steamed vermiculite. After 7 days of cultivation, the upper part 2 cm in length of cucumber seedling, was cut and used as cucumber cuttings. The base of cutting stem was then dipped in a microcentrifuge tube containing 1.5ml of a bacterial suspension and incubated at $25^{\circ}C$ with a fluorescent light for 10 days. Number and length of developed adventitious roots from cucumber cuttings were examined. The seedling cuttings showed various responses to the isolates tested. Some isolates resulted in withering at the day of examination or in reduced number of roots developed. Several isolates stimulated initial development of adventitious roots showing more adventitious root hair number than that of untreated cuttings, while some isolate had more adventitious root hair number and longer adventitious roots than that of untreated control. Similar results were obtained from the trial with rose cuttings. Our results suggest that this bioassay method may provide a useful way for differentiating PGPR's functions involved in the development of root system.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.97-102
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• 2003

Lettuce (Lactuca sativa) was transformed with Agrobacterium tumefacience LBA4404 containing human granulocyte macrophage colony stimulating factor (hGM-CSF) gene to produce in cell suspension cultures. Cell suspension culture was established using callus from transgenic lettuce plant. Integration of hGM-CSF gene into plant chromosome was confirmed through genomic PCR and Southern blot analysis. In addition, Northern blot analysis indicated the expression of the introduced hGM-CSF gene in transformed lettuce. The recombinant hGM-CSF was expressed in transgenic cell cultures derived from transgenic plants as a yield of about 149.0 $\mu\textrm{g}$/L in culture filtrate, which was determined by ELISA. These results demonstrated that transformed lettuce cell suspension cultures could be used as a production system of therapeutic proteins such as hGM-CSF.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2019.11a
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• pp.42-43
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• 2019

In-situ production of PC (precast concrete) members can reduce costs by about 14.5% -21.6% compared to in-plant production due to the reduction of transportation costs, factory profits and overhead costs. However, in-situ production of PC members presents a variety of risks, including member production and yard area securing, and lead time for production within the installation period. To solve this, it is necessary be able to analyze and control and monitor the risk factors that influence in-situ production for PC member. The purpose of this study is to develop a dynamic simulation model for in-situ production and erection integrated management for PC members. For this study, risk factor identification, causal loop diagram, and dynamic simulation model construction were performed sequentially. The results of this study will be used as a basis for developing a risk management model for PC in-situ production.

• Proceedings of the Korea Concrete Institute Conference
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• 1998.04a
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• pp.111-116
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• 1998

This studies regarding development of product system on artificial aggregate using of Paper Sludge Ah that waste production at paper-making mill, expecially this paper describes development of product system using centrifugal mixer plant. The skills of this product system on artificial aggregate using of Ash can be spread Fly-Ash, powder and fine aggregate(under 5mm) of waste concrete reuse.

• Journal of Plant Biotechnology
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• v.47 no.2
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• pp.107-117
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• 2020

Flower industry is now growing due to the development of economy in many countries. Simultaneously, needs from consumers in flower market are varied widely. To satisfy the needs from consumers and deal with a variety of diseases from a lots of pathogens as well as climate change, new elite flower cultivars should be released in flower market. For this purpose, conventional and biotechnological techniques can be employed to make good cultivar. Therefore, this review describes the general overview of flower breeding techniques including cross-hybridization, mutation breeding and genetic transformation systems. Also, breeding systems for ornamentals derived from plant tissue culture such as embryo culture, in vitro fertilization, ovary/ovule culture and haploid production were reviewed. Furthermore, in this study recent development of the generation of new flower cultivars using marker-assisted breeding, plant transformation including particle bombardment and Agrobacterium tumefaciens as well as genome-editing technology were described. This review will be contributed to the development and releasement of new flower cultivars with horticulturally useful traits in the future.

• 제어로봇시스템학회:학술대회논문집
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• 1992.10b
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• pp.74-78
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• 1992

This paper describes an intelligent system to automatic evaluation of eddy current(EC) signal for Inspection of steam generator(SG) tubes in nuclear power plant. Some features of the intelligent system design in the proposed system are : (1) separation of representation scheme ,or event capturing knowledge in EC signal and for structural inspection knowledge in SG tubes inspection; (2) each representation scheme is implemented in different methods, one is syntactic pattern grammar and the other is rule based production. This intelligent system also includes an data base system and an user interface system to support integration of the hybrid knowledge processing methods. The intelligent system based on the proposed concept is useful in simplifying the knowledge elicitation process of the rule based production system, and in increasing the performance in real time signal inspection application.

• Plant Journal
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• v.12 no.2
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• pp.36-40
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• 2016

At the contract for power plant construction, the penalty appropriation on performance decrease is signed between ordering organization and construction firm. In this, the penalty cost signed must be reasonable value that both of ordering organization and construction firm can accept, therefore the methodology for penalty appropriation is very important. Cogeneration is a system that produces electricity and heat at the same time, therefore the penalty appropriation for cogeneration should be uncertain. Thermoeconomics analyzes various energy costs, however the relation of thermoeconomics and penalty cost may not be analyzed up to now. The aim of this study demonstrates that thermoeconomics can be applied to the penalty appropriation at the performance acceptance test. As the result of CGAM system, if the construction cost is $10,000,000, the value of $6,665,688 was appropriated to the electricity production performance and the value of $3,334,312 was appropriated to the heat production performance. Therefore if one percentage at the electricity production performance decreases, the penalty is $6,666, and one percentage at the heat production performance decrease, we can understand that the penalty is $3,334.

• Journal of Hydrogen and New Energy
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• v.35 no.4
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• pp.353-362
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• 2024

In this study, several studies were conducted on the construction of gas conversion process system for a pilot plant using a small-scale hydrogen liquefaction system. The pilot plant considered in this study includes a liquefier, a storage tank, an evaporator, a gas booster, and a gas storage tank. First, the suspected leak area of the container was checked using the sprayed method of helium gas. The small-scale hydrogen liquefaction system was designed based on the analysis results of the pre-cooling system and the liquefaction system. Additionally, the program was developed to maintain pressure within vessel for an automatic production of liquid hydrogen. The evaporator for liquid hydrogen was manufactured based on the designed analysis data, and the pollution of gas in the vessel was analyzed through a gas recovery line system.