• Applied Microscopy
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• v.50
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• pp.10.1-10.6
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• 2020

Plant specimens for scanning electron microscopy (SEM) are commonly treated using standard protocols. Conventional fixatives consist of toxic chemicals such as glutaraldehyde, paraformaldehyde, and osmium tetroxide. In 1996, methanol fixation was reported as a rapid alternative to the standard protocols. If specimens are immersed in methanol for 30 s or longer and critical-point dried, they appear to be comparable in preservation quality to those treated with the chemical fixatives. A modified version that consists of methanol fixation and ethanol dehydration was effective at preserving the tissue morphology and dimensions. These solvent-based fixation and dehydration protocols are regarded as rapid and simple alternatives to standard protocols for SEM of plants.

• Food Science and Preservation
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• v.30 no.6
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• pp.999-1011
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• 2023

This study investigated the anti-inflammatory, trypsin activity, and antioxidant effects of 11 kinds of plant extracts to discover materials for developing optimal mixtures that improve inflammation and help digestion. Ziziphus jujuba Mill. (ZJ), Leonurus japonicus Houtt. (LJ), Scutellaria baicalensis (SB), Platycodon grandiflorum, and Aster scaber extracts had excellent anti-inflammatory effects by reducing excessive nitric oxide (NO) and tumor necrosis factor-α content in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The malt (MA), Pyrus pyrifolia Nakai (PP), Raphanus sativus L., Platycodon grandiflorum extracts among the 11 kinds of plant extracts had high trypsin activity. The antioxidant activity of the plant extracts was examined by the DPPH radical scavenging activity, and the SB, PE, JU, and MA extracts had high antioxidant activity. Therefore, PP, MA, ZJ, LJ, and SB were selected to develop optimal mixtures that improve inflammation and help digestion. The extract of plant mixture containing PP, MA, ZJ, LJ, and SB in the ratio 1:1:2:1:2 (w/w) significantly inhibited NO production than the extract of PP, MA, ZJ, LJ, and SB, respectively, in LPS-stimulated RAW 264.7 macrophages. The DPPH radical scavenging activity of the mixture extract was significantly higher than the extract of PP, MA, ZJ, and LJ, respectively.

• Food Science and Preservation
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• v.31 no.2
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• pp.298-306
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• 2024

This study evaluated the differentiation and protective effects of mixed plant-extract powder in C2C12 muscle cells. Cells were differentiated into myotubes in 2% horse serum (HS)-containing medium with mixed plant-extract powder (MPEP) for 6 days. Treatment with MPEP increased the expression of myogenin and myosin heavy chain (MHC) protein in cells compared with non-treated cells. Differentiated cells were pretreated with MPEP, and hydrogen peroxide (H₂O₂). Our results revealed that treatment with MPEP before H₂O₂ treatment increased cell viability and decreased H₂O₂-induced lactate dehydrogenase (LDH) and creatine kinase (CK). In addition, MPEP attenuated H₂O₂-induced upregulation of Bax, downregulation of Bcl-2, and activation of caspase-9 and -3. These results suggest the MPEP can stimulate C2C12 muscle cell differentiation into myotubes and observe the protective effect of mixed plant-extract powder against muscle oxidative stress. In conclusion, MPEP may be useful as a prevention and treatment material for skeletal muscle disease caused by age-related diseases.

• Journal of Conservation Science
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• v.33 no.5
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• pp.363-370
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• 2017

The aim of this study was to obtain information about two early-20th Century clothes, for which the "National Memorial Museum of Forced Mobilization under Japanese Occupation" has sought to receive preservation treatment. Optical microscopes and a scanning electron microscope were used to investigate the weaving of the clothes, and Fourier transform infrared spectroscopy(FT-IR) was used to investigate the fibers. Cloth A is believed to be a Japanese half sleeved inner wear(Hanjuban) used by women. Cloth B is believed to be working clothing that was checked by an Osaka plant. This was verified by a book written by the Japanese army. Both of the clothes were made mostly from cotton, although the inner wear also used viscose rayon on the neck collar. The button on the working wear was made of urea formaldehyde resin, an early precursor to plastic.

• Mycobiology
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• v.46 no.2
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• pp.122-128
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• 2018

Arbuscular mycorrhizal fungi (AMF) are well-known for their ability to improve plant growth and help plants withstand abiotic stress conditions. Unlike other fungi and bacteria, AMF cannot be stored, as they are obligate biotrophs. Long-term preservation of AMF spores is challenging and may lead to the loss of viability and efficiency. This study aimed to understand the effect of prolonged subculture of AMF species on the growth and glomalin-related soil protein (GRSP) from red pepper (Capsicum annuum L.). AMF spores were mass-produced using different techniques and subcultured in pots with sorghum sudangrass as the host plant for 3 years. Experimental soil samples were collected from natural grassland. Five different AMF inocula were used in triplicate as treatments. After 70 days of growth, red pepper plants were harvested and plant dry weight, plant nutrient content, mycorrhizal colonization, AMF spore count, and soil glomalin content were determined. AMF-treated plants displayed higher dry weight than controls, with only fruit dry weight being significantly different. Similarly, significant differences in phosphorous and potassium contents of the above-ground plant parts were observed between mycorrhizal and control treatments. In addition, soil GRSP content was significantly higher in plants inoculated with Rhizophagus sp. and Gigaspora margarita. The increased plant growth and GRSP content suggest that AMF can be maintained for 3 years without losing their efficiency if subcultured regularly with different symbiotic host plants.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.51-52
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• 2017

"Plant genetic resources for food and agriculture" means any genetic material of plant origin of actual or potential value for food and agriculture. "Genetic material" means any material of plant origin, including reproductive and vegetative propagating material, containing functional units of heredity. (Internal Treaty on Plant Genetic Resources for Food and Agriculture, ITPGRFA). The "Nagoya Protocol on Access to Genetic Resources and the Fair and Equitable Sharing of Benefits Arising from their Utilization (ABS) to the Convention on Biological Diversity (shortly Nagoya Protocol)" is a supplementary agreement to the Convention on Biological Diversity. It provides a transparent legal framework for the effective implementation of one of the three objectives of the CBD: the fair and equitable sharing of benefits arising out of the utilization of genetic resources. The Nagoya Protocol on ABS was adopted on 29 October 2010 in Nagoya, Japan and entered into force on 12 October 2014, 90 days after the deposit of the fiftieth instrument of ratification. Its objective is the fair and equitable sharing of benefits arising from the utilization of genetic resources, thereby contributing to the conservation and sustainable use of biodiversity. The Nagoya Protocol will create greater legal certainty and transparency for both providers and users of genetic resources by; (a) Establishing more predictable conditions for access to genetic resources and (b) Helping to ensure benefit-sharing when genetic resources leave the country providing the genetic resources. By helping to ensure benefit-sharing, the Nagoya Protocol creates incentives to conserve and sustainably use genetic resources, and therefore enhances the contribution of biodiversity to development and human well-being. The Nagoya Protocol's success will require effective implementation at the domestic level. A range of tools and mechanisms provided by the Nagoya Protocol will assist contracting Parties including; (a) Establishing national focal points (NFPs) and competent national authorities (CNAs) to serve as contact points for information, grant access or cooperate on issues of compliance, (b) An Access and Benefit-sharing Clearing-House to share information, such as domestic regulatory ABS requirements or information on NFPs and CNAs, (c) Capacity-building to support key aspects of implementation. Based on a country's self-assessment of national needs and priorities, this can include capacity to develop domestic ABS legislation to implement the Nagoya Protocol, to negotiate MAT and to develop in-country research capability and institutions, (d) Awareness-raising, (e) Technology Transfer, (f) Targeted financial support for capacity-building and development initiatives through the Nagoya Protocol's financial mechanism, the Global Environment Facility (GEF) (Nagoya Protocol). The Rural Development Administration (RDA) leading to conduct management agricultural genetic resources following the 'ACT ON THE PRESERVATION, MANAGEMENT AND USE OF AGRO-FISHERY BIO-RESOURCES' established on 2007. According to $2^{nd}$ clause of Article 14 (Designation, Operation, etc. of Agencies Responsible for Agro-Fishery Bioresources) of the act, the duties endowed are, (a) Matters concerning securing, preservation, management, and use of agro-fishery bioresources; (b) Establishment of an integrated information system for agro-fishery bioresources; (c) Matters concerning medium and long-term preservation of, and research on, agro-fishery bioresources; (d) Matters concerning international cooperation for agro-fishery bioresources and other relevant matters. As the result the RDA manage about 246,000 accessions of plant genetic resources under the national management system at the end of 2016.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.04a
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• pp.109.2-110
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• 2003

The neuroprotective effect of solvent fraction of native edible plant MK-104 in the mice administered with kainate was evaluated using behavioral sign, neuronal injuries and biomarkers of oxidative stress. Mice, ICR male, were administered with the BFME through a gavage for 4 days consecutively, and on the 3rd day, kainate (450 mg/kg) was i.p. administered. The fraction(400 mg/kg) delayed the onset time of neurobehavioral change (p<0.01), reduced the severity of convulsion and lethality (p<0.05), and restored the level of GSH and lipid peroxidation in brain to control value. A similar protective action was also expressed by fraction-I (200 mg/kg), which showed a prominent protection against the neuronal damage in hippocampal CA1 and CA2 regions (p<0.01) caused by kainate injection. of TBARS value. Based on these results, BFME-I is suggested to contain a functional agent to prevent against oxidative stress in the brain of mice.

• Journal of Plant Biology
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• v.11 no.4
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• pp.21-29
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• 1968

It may be fully known that Korea is the main producing country of pine tree form the pan-Asian viewpoint of the geographical distribution of the pine tree. Through the study on the reason why the pine tree became a tree of the maximum cover degree in Korea and the observation of natural preservation status of all sorts of trees in the DMZ area for nearly 20 years after the Korean Armistice, it has come to know that almost all of the pine tree were banished. This phenomenon has been resulted by the ecological characteristics of the pine tree itself which has no sprouting power at all, war disasters, deforestation and incendiary fire that have been steadily occurred until now since the time of Armistice.

• Journal of Environmental Impact Assessment
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• v.21 no.3
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• pp.431-443
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• 2012

Wind holes or air holes, from which cool air blows out during the summer, but mild air comes out during the winter, have provided the phytogeographically important refugia for cryophilous or cold-loving boreal flora during the Holocene period. At present, wind holes are serving as a faraway disjunctive habitat for Pleistocene relict glacial floristic elements, and present an invaluable information to reconstruct the natural history. Present work aims to collate the nationwide distribution and relevant DB on the potential wind holes of Korea based upon media and literature sources, along with geographical informations, such as place name, topographic map, environmental geographical information, flora, monitoring data of Korea National Arboretum, and field survey data. Geographical information on sixty nine wind hole sites have compiled and analyzed on the basis of flora and presence of fossilized periglacial landforms, such as talus, block field, and block stream, and sixteen sites have thereafter carefully selected and scrutinized through field surveys. To maintain a sustainability of wind hole ecosystem, including their original landform, micro-meteorological phenomena and plant community therein as a refugia or habitat for relict plant species of Pleistocene glacial period, appropriate restrictions and preservation measures are required.

• Food Science and Preservation
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• v.13 no.3
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• pp.279-284
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• 2006

This study was carried out to investigate the qualities of Saururus chinensis Baill. rhizomes and it content of active ingredient accenting to the storage methods. As the storage period increased the rates of weight loss, rotten rhizomes and the number of sprouts increased. Refrigerator storage resulted in the lowest weight reduction and sprouts rates, while storage in soil-filled box in a store-house was most effective in reducing spoilage rate. Five lignans from rhizome were determined by HPLC Retention time ranged $18{\sim}36$ minutes and showed saucernetin sauchinone, manassantin A, saucerneol D, and manassantin B in that order. Regardless of storage methods, the lignan content was lower after 120 days than after 30 days of storage, and increased manassantin B, manassantin A, saucernetin sauchinone, and saucerneol D in that order.